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Rhodobacter Capsulatus Porphobilinogen Synthase, A High Activity Metal Ion Independent Hexamer, David W. Bollivar, Cheryl Clauson, Rachel Lighthall, Siiri Forbes, Bashkim Kokona, Robert Fairman, Lenka Kundrat, Eileen K. Jaffe
Rhodobacter Capsulatus Porphobilinogen Synthase, A High Activity Metal Ion Independent Hexamer, David W. Bollivar, Cheryl Clauson, Rachel Lighthall, Siiri Forbes, Bashkim Kokona, Robert Fairman, Lenka Kundrat, Eileen K. Jaffe
David Bollivar
Background: The enzyme porphobilinogen synthase (PBGS), which is central to the biosynthesis of heme, chlorophyll and cobalamins, has long been known to use a variety of metal ions and has recently been shown able to exist in two very different quaternary forms that are related to metal ion usage. This paper reports new information on the metal ion independence and quaternary structure of PBGS from the photosynthetic bacterium Rhodobacter capsulatus. Results: The gene for R. capsulatus PBGS was amplified from genomic DNA and sequencing revealed errors in the sequence database. R. capsulatus PBGS was heterologously expressed in E. coli and …