Digital Commons Network^™

Dimerization Of Dopamine (D2) And Adenosine (A2a) Receptors, Elena Vukovic, Jackson M. Macatol

Williams Honors College, Honors Research Projects

This experiment will aim to explore the heterodimerization of Dopamine (D2) and Adenosine (A2A) receptors in cells when bound to different ligands. Borroto-Escuela et al. (2018) investigated the dimerization of the A2AR-D2R in rats when administered with cocaine, and they found that disrupting the A2AR-D2R complex restores the cocaine reward system in rats. This was a strong influence as to why we chose to investigate A2AR-D2R dimerization in Chinese hamster ovary (CHO) cells.

One goal that we have for this experiment is to combine our current laboratory skills with new laboratory techniques. There are constantly new techniques in labs, and …

The Use Of Dynamic Light Scattering To Determine Mineral Precipitation In Bacteria., Isabella Manning

Williams Honors College, Honors Research Projects

Particulate calcium carbonate (PCC) is an important filler in industrial products, including paints, which are obtained through mining and sintering (heating to 1,100°C), which is responsible for up to 2% of global emissions. A potential solution to reduce these emissions is the use of calcium carbonate produced by bacteria from atmospheric , through a process called microbially induced carbonate precipitation (MICP). One of the primary uses of PCCs is in paint, requiring them to be a specific size (~700nm). When we have been collecting samples of PCCs produced by bacteria, which demonstrate clumping, making them too large for use in …

Uncovering A Natural History Mystery At The University Of Akron, Gary M. Holliday, Lara Roketenetz

Proceedings from the Document Academy

Students were engaged in a combination of detective work, biology, archives, art, and education/outreach to prepare a collection of taxidermied birds donated to the University of Akron by the Rhodes family in the early 1900s for public access and exhibition. Students had the opportunity to learn from leading experts regarding the historical significance of biological collections, proper preservation protocols, digitizing and archival practices, and the urgency of science education and communication for a public audience. Many campus and community partners were involved, including experts from local museums and nature centers.

Attitudes Toward Transgenic Corn Usage Among Amish & Conservative Mennonite Farmers In Ohio, Scot Long

Journal of Amish and Plain Anabaptist Studies

Mass adoption and planting of genetically modified corn are part of the larger industrialized agricultural production system in the United States. Amish and conservative Mennonite farmers in the Holmes County settlement region offer an alternative production system often characterized by lower usage of chemical inputs, greater implementation of crop rotation, and significantly higher usage of hybrid versus GMO field corn. Moreover, the rationale among Amish/Mennonite farmers toward adoption of GMO (based on “convenience”) or rejection of GMO (based on “too many unknowns”) stems both from cultural diffusion of neighboring farms as well as variable need for nonfarm income. This article …

Potential Effects Of Amynthas Agrestis Invasion On Woody Understory Flora In The Cvnp, Christian Mammana

Williams Honors College, Honors Research Projects

Ohio forests are threatened by the invasive ecosystem engineer A. agrestis. A. agrestis invasion typically co-occurs with the ecosystem engineer, Odocoileus virginianus, where their impacts may synergize. To determine the direct effects of A. agrestis invasion, fenced plots across the Cuyahoga Valley National Park that excluded deer were utilized. The species richness, Shannon diversity and evenness of woody understory flora was measured in each plot. Mustard extraction was used to determine earthworm abundance. Correlations between abundance and measured variables were used to highlight potential invasion effects. Abundance and species richness was found to have a significant, positive correlation (p = …

How Will The Expanding Agriculture Business Impact Wetland Graminoids?, Travis Darago

Williams Honors College, Honors Research Projects

As human population increases in the years to come so too will the agriculture business. We already know that the use of chemicals such as fertilizers and other agriculture chemicals aid to promote accelerated crop growth so that we can harvest the most food for the most people. There is less research on the effects that the runoff of these chemicals have on wetland environments that are downstream from farmland. I would like to explore how some of these agricultural chemicals, like ammonium, will affect the growth of different types of wetland graminoids. I will be looking at Poa polustris, …

Immunohistochemistry Of The Zebrafish Retina, Alison Ruf

Williams Honors College, Honors Research Projects

The retina is made up of many different essential parts that work together to allow for vision of many different species. Some of these crucial cells and proteins include ganglion cells, bipolar cells, photoreceptors, synaptic markers, such as VGLUT, and receptors that bind glutamate. The basis of this project is to use immunohistochemistry to identify these important aspects of the retina in zebrafish via monoclonal and polyclonal antibodies. The goal being to compare specific cells in a control group retina to an experimental group of zebrafish.

Intuitive Eating Behaviors Among A Sample Of Food Secure And Food Insecure College Students, Morgan Stocker, Christin Seher Phd, Rdn, Ld

Williams Honors College, Honors Research Projects

Intuitive Eating (IE) is a food mindset focusing on honoring hunger and fullness, while rejecting the diet mentality. Research demonstrates a clear link between IE and health outcomes across many populations, including college students. Despite a growing body of literature, we still know very little about what complexities food insecurity may present for individuals trying to adopt IE behaviors. This study builds off prior research to investigate IE behaviors in food insecure and food secure students through a Qualtrics survey utilizing the Intuitive Eating Scale-2 (IES-2) and the United States Department of Agriculture (USDA) Six-Item Short Form to determine food …

"Demeter" Soil Monitoring System, Ryan Matthews, Rachel Rummer, Temilolu Fayomi, Alex Fuller

Williams Honors College, Honors Research Projects

The purpose of this project is to develop a soil monitoring system that can remotely sense and relay soil conditions back to a user. The deMETER soil probe, Demeter is the Greek goddess of the harvest, is designed to aid hobbyist gardeners, small-scale farms, and nurseries to monitor their dynamic soil conditions and maximize their harvest. The probe is a self-powered system that can monitor the moisture and essential nutrients of the soil profile to determine which areas should receive water and fertilizer. This would significantly cut water and fertilizer waste. The solution will include an embedded system with sensors …

Chemogenetic Induced Tinnitus Model, Cara Bunner

Williams Honors College, Honors Research Projects

This project will use chemogenetic drug activation in the inferior colliculus (IC) to formulate a new tinnitus model in animals. Gq-signaling DREADD [AAV8-CaMIIKa-HDM2(Gq)-mCherry] will be used to selectively activate glutamatergic neurons in the mouse, then the brain will be dissected to obtain the IC. The IC will be immunohistochemically stained to visualize the AAV mCherry, the glutamatergic neuron distribution, and the GABAergic neuron distribution within the IC. Thus, the findings of this study will better our understandings of the pathological mechanisms of tinnitus.

Conservation Of Lapping Mechanism In Marsupials, Angela Brkic

Williams Honors College, Honors Research Projects

The oral behavior of drinking includes interactions between the tongue, jaw, and liquid being ingested to provide hydration to the animal. Different mechanisms may be used to ingest liquid such as lapping, licking, or sucking. The objective of the study was to compare the mechanism of lapping across marsupial species through comparison of the length of gape cycle, and the amplitude of jaw pitch and tongue protraction between the two marsupial species, Didelphis virginiana and Monodelphis domestica. I hypothesized that there would be no differences in any of the variables for the lapping mechanism between species. Previous work demonstrates …

Glaucoma Alters The Morphology Of Mouse Melanopsin-Containing Retinal Ganglion Cells, Kristin Keenan

Williams Honors College, Honors Research Projects

Melanopsin ganglion cells are intrinsically photosensitive ganglion cells in the mammalian retina. These cells mediate aspects of non-image forming vision to help regular circadian rhythm. Glaucoma is an eye disease involving an increase in intraocular pressure that has been linked to irregular circadian rhythm. This project will investigate the impact glaucoma has on these melanopsin ganglion cells at different pathological timepoints. This will be performed by using dissection, immunohistochemistry, and computer imaging methods to visualize, trace, and measure the cells’ morphologies in both control and glaucomatous retinas at different timepoints of disease to analyze if glaucoma has a significant effect …

Mimicking The Namib Desert Beetle With Hydrophilic And Hydrophobic Surfaces, Tyler Bracewell

Williams Honors College, Honors Research Projects

The Namib Desert beetle, Stenocara gracilipes, condenses fog that rolls in off the west coast of Namibia to provide itself with the water it needs for the day. By lifting its abdomen into the air to collect the water vapor, it condenses on hydrophilic bumps that are about 500μm in diameter and are spaced apart about 500-1500μm. Once the water reaches a critical size, the droplet rolls off the hydrophilic bump and onto the flat hydrophobic surface of the elytra. Since the beetle is angled with its abdomen in the air, the water rolls down to its mouth. A …

Western Diet In Adulthood, Timing Of Menarche, And Economic Status Among Northeast Ohioans, Kailey Maroni, Anne Wiley Phd, Nicole M. Burt Phd, Randall J. Mitchell Phd

Williams Honors College, Honors Research Projects

I studied the age at first menstruation as a predictor of Western diet consumption among adults at The University of Akron and in Cleveland. I used stable carbon and nitrogen isotope compositions of human hair, as well as surveyed consumption of foods important to a Western diet (red meat, sugar, and dairy), to understand whether participants aligned with a Western dietary pattern. Age at survey and adult economic status were investigated as potential confounding variables. My data analysis and conclusions for this project has been completed and approved by my advisor and committee. To maintain collaborators' ability to publish a …

Garden Bot: Autonomous Home Garden Weed Removal Robot, Brendon Lovejoy, Robert Connolly, Isaac Lucas, Stevan Veselinov

Williams Honors College, Honors Research Projects

With frequent weeding being a tedious chore and an essential task for a successful garden, there is need for an automated method of handling this routine. Existing technologies utilize computer vision, GPS, multiple units and other tools to remove weeds from garden plots. However, these solutions are often complex and expensive, suited for large agricultural plots in contrast to small-scale home gardens. In addition, many of these technologies, along with manual tillers and cultivators suited for home use, are unable to perform weeding within rows of crops in a process known as intra-row weeding. The Garden Bot is an autonomous, …

Will Covid-19 Be Eradicated?, Matthew Kieke

Williams Honors College, Honors Research Projects

In this paper I want to define the meaning of “eradication”, explain the history of various diseases that have been eradicated, and compare and contrast them to covid-19, to answer the question of “Will covid-19 be eradicated”?

Autonomous Coffee Brewing System, Lillian Gonzalez, Landon Miller, Tyler Moff, Benjamin Vitu

Williams Honors College, Honors Research Projects

Coffee consumers want an affordable, high-quality cup of coffee that takes minimal time to brew and does not excessively contribute plastic to landfills. A fully autonomous system which utilizes whole coffee beans, water, and filters to brew coffee was designed to meet this need. This system accepts user requests which specify the brew’s portion size, water temperature, and brew strength, as well as the time which the system should begin the brewing process. Occasional maintenance to empty the system’s built-in waste receptacle or resupply the system with water, coffee beans, or filters is the only user intervention required. This report …

The Effects Of Average Annual Temperature On Flowering Times And Flower Count, Angela Copploe

Williams Honors College, Honors Research Projects

Over a course of 15 years, three different species of flowers were examined to see the relationship between the increase in average annual temperature, flowering time, and flower count.

Functionalization Of Chitosan Based Microparticles For In Vitro 3d Culture Of Human Liver Cells, William Imes

Williams Honors College, Honors Research Projects

Previous work involving 3D culture of human liver cells with fluorinated chitosan based microparticles has shown that their incorporation provides needed structural cues that the culture of cells alone does not provide, such as increased gas transport. However, using bare microparticles to grow large 3D cellular structures is not practical as they tend to collapse before any meaningful research can be done on them. It is proposed that this is because of the lack of extracellular matrix (ECM)components within pure cell culture in vitro, which in their absence cannot adequately facilitate biochemical communication and adhesion between cells. It is …

Effect Of An Acl Tear On Bone Density And Muscle Mass Contralaterally, Rebecca Beitko, Carly Anenson

Williams Honors College, Honors Research Projects

The research question that this experiment aims to answer is: "How does bone density and muscle mass of the upper and lower leg differ contralaterally in individuals who have had an anterior cruciate ligament (ACL) tear?" This project will examine how an ACL injury affects the bone density and muscle mass of both the upper and lower leg by comparing these values in the injured vs. the uninjured leg. We will acquire bone density and muscle mass values using a DEXA scan (x-ray imaging). In comparing the contralateral values, we will be able to identify if individuals who have suffered …

Sequential Block Copolymers Of Polyisobutylene And Polyolefins, Manwel Labib

Williams Honors College, Honors Research Projects

A two-step approach will be utilized to synthesize random polyolefin multiblock-copolymers. This strategy takes advantage of a,w-terminated telechelic polyolefins. Polypropylene (iPP)/polyethylene (PE) are picked because of their strength, and polyisobutylene (PIB) because of its hemocompatibility. This copolymer is theorized to make for robust bio-inert plastic, which can be used for surgical mesh implants.

Biodegradation Of Rubber Particles In Soil: Using Acclimated Bacteria Isolated From Kansas Soil To Degrade Cryogrinds In Slurry, Shane Graham

Williams Honors College, Honors Research Projects

This study investigated the viability of bioremediating rubber cryogrind using enriched indigenous bacteria. To begin the experiment, soils from three highway roadside locations in Kansas, KS 96 and West, KS 400 and 143^rd, and 199^th, were collected and transported to the lab to be studied. An initial soil characterization was run on the soil samples using distilled (DI) water mixture and 0.01 M CaCl₂ to assess conductivity. The soils were tested to gather a baseline of the relationship between pH and conductivity and the impact of its distance from the roadside. Bacteria were isolated from …

Effect Of Glaucoma On Melanopsin Retinal Ganglion Cells In Mice, Abigail Mcmullen

Williams Honors College, Honors Research Projects

Within the mammalian retina, there are melanopsin-containing ganglion cells. These intrinsically photoreceptive cells help control behavioral aspects of vision as well as to aid in circadian rhythms. In this project, it will be determined whether or not glaucoma has an effect on these cells, and what that effect is, from different points in the disease. Glaucoma is an eye disease that damages the retinal ganglion cells and eventually causes them to die, also it can be linked to irregular circadian rhythm. Many techniques will be used to prepare this project but computer imaging will be the primary method for determining …

The Influence Of Hydrogen Peroxide On The Enrichment Of Fe(Iii) Reducing Bacteria From Acid Mine Drainage, Susami Seth

Williams Honors College, Honors Research Projects

It is hypothesized that the ocean of Europa, a Jupiter moon, hosts bacteria on its oceanic floor. Understanding how Fe(III) reducing bacteria (FeRB) from AMD utilize organic materials within its surrounding environment outlines how FeRB could thrive and tolerate extreme conditions. FeRB are known to tolerate metals and highly reactive oxidants species (ROS), but in this experiment, H₂O₂ was the experimental factor to further test FeRB tolerance. H₂O₂ is a common ROS and is damaging to living material such as proteins, DNA, and RNA. A range of H₂O₂ concentrations were fed …

Synthesis Of Rna Nucleotides Under Probable Prebiotic Conditions, Ryan Stimson

Williams Honors College, Honors Research Projects

RNA being composed of multiple covalently linked nucleotides is thought to have been a precursor to life circa 4.3-3.8 billion years ago. Non-enzymatically formed adenosine monophosphate (AMP), more specifically, is a vitally important subtopic of the self-assembly of the first RNA sequence. The goal of this study was to synthesize AMP non-enzymatically under benign conditions that are likely to have existed on early Earth. In this experiment, 3’,5’-cAMP was successfully formed using wet-dry cycles at 80°C paired with the minerals zeolite beta, hydroxyapatite, and aerosil 300 in the presence of adenosine, urea, and pyrophosphate. A nuclear magnetic resonance spectrometer was …

Penn State Extension Services And Plain People: An Inside Perspective On A Trust-Based Relationship, Phillip Martin

Journal of Amish and Plain Anabaptist Studies

This perspective on Penn State Extension Services and plain people is based on my personal experiences as a plain person, in which I interacted with Extension Services first as a farmer, and then (while working on a doctorate) as a part of the Extension system. Penn State Extension started over a century ago and was deliberate in reaching out to plain (conservative Anabaptist) farmers since the beginning, which led to a history of trust-based cooperation. For all these successes there remain challenges to effective cooperation with certain plain individuals and subgroups. I suggest these challenges are broadly similar to those …

Hotline Number To Reach And Offer Agricultural Information To Plain Anabaptists During The Covid-19 Workplace Closures, Emily Shoop, Carly Becker, Nathan Glenn Briggs, Danielle Smarsh

Journal of Amish and Plain Anabaptist Studies

The Penn State Extension Animal Systems Team developed a toll-free hotline phone number to convey short educational messages to maintain contact and outreach to Pennsylvania’s animal producing Amish, Mennonite, and other plain Anabaptist populations. During the COVID-19 pandemic, Penn State Extension’s programming went largely online via webinars, online courses, and emails. This change in programming excluded a large sector of Pennsylvania farmers who do not use or have access to the internet and, as such, were liable to miss important best management practice reviews and timely updates in animal agriculture. The Animal Systems Hotline offered callers the choice to listen …

Agricultural Support For The Old Colony Mennonites Of Belize: A Collaborative Effort Between North American Old Order Mennonites And A Cornell Extension Educator, Judson Reid

Journal of Amish and Plain Anabaptist Studies

The Old Colony Mennonites of Belize practice a conservative form of Anabaptism where technology, dress and lifestyle choices are informed by church standards. Members live in a spatially contiguous Colony, which creates land pressure over time as population increases. The development of new Colonies is necessary for the Mennonites to participate in agricultural livelihoods. The Indian Creek Colony of Belize has experienced agricultural and economic hardships since its inception in the 1989 due to land costs, crop failures and debt structure. Old Order Mennonites of North America developed an outreach effort to assist with on-farm production of vegetable crops to …

Providing Agricultural Information To Amish And Mennonite Farmers: Creative Adaptations To Wisconsin Extension Programs And Communications, Richard Halopka

Journal of Amish and Plain Anabaptist Studies

This service provider report taps into the experience of a longtime Extension Educator in Wisconsin. Pulling from his work in Clark County, WI, where Amish and Old Order Mennonites have been particularly important to the local agricultural economy, he provides examples of how Extension can modify programming to meet the needs of plain producers. Topics of interest to Amish and Mennonite farmers include nutrient management, forage quality, and farm safety. Areas of program modification include approaches to communication, training, and support in ways that are culturally sensitive to church restrictions. In-person training and informational voicemail recordings have been particularly useful …

Effective Communication And Programming When Working With Amish Farmers: Reflections From A Wisconsin Agriculture Educator, Vance Haugen

Journal of Amish and Plain Anabaptist Studies

The author, an agricultural educator who worked with the Amish in Wisconsin for over 30 years, discusses his outreach efforts, which have been focused on managed grazing, a method well suited to Amish producers. Managed grazing offers agronomic, economic, and ecological benefits. A key educational tool for communicating managed grazing practices is the pasture walk, and the author relates lessons learned from these events. The communal nature and focus on farmer-to-farmer information exchange is well received by Amish producers. It is helpful if pasture walk scheduling is done well in advance and is focused on farmer-based problems with farmer-based solutions …