Digital Commons Network^™

The Kinematics Of Salamander Underwater Walking On Smooth And Rough Substrate, Sophie Cressman

Williams Honors College, Honors Research Projects

The transition from aquatic to terrestrial locomotion was a landmark event in the evolutionary history of vertebrates. In order to better understand how early tetrapods were able to transition to land, salamanders have proven to be a useful animal model. With current research focusing heavily on the anatomy, physiology, and kinematics defining salamander terrestrial walking capabilities, this experiment was designed to extract information about the kinematics of salamander walking underwater. Underwater walking of salamanders was analyzed on smooth and rough substrates to provide more insight into the early vertebrate water-to-land transition. Average salamander stride duration, time in stance phase, time …

Sports Nutrition Newsletters, Michelle Dilling

Williams Honors College, Honors Research Projects

The purpose of my project is to teach athletes that nutrition is important to their performance, to introduce them to new topics, and to separate fact from fiction by citing evidence-based research. Additionally, the purpose of my project is to design a template for this newsletter that can be used to continue publications.

Inferring Species Origin Through Virtual Histology: A Comparison Of Third Metapodials From Homo Sapiens And Ursus Americanus Using Micro-Computed Tomography, Hannah Stephen

Williams Honors College, Honors Research Projects

Human and black bear metacarpals and metatarsals, or the hand and foot bones and bear paws, are well documented on a gross anatomical level. The macrostructural data comparing the two species is useful in distinguishing the species unless fragmented bone is found. In this case, histological data is used for clearer and more accurate comparisons but, research that compares the metapodials of black bears and humans on a microstrutural level is scarce. This study aimed to aid in forensic science identification scenarios where fragmented bone is discovered by providing the differences between black bear and human metacarpals and metatarsals on …

Mutations In Rhodopsin Transmembrane Helix 5 And Dimerization Interface, Margaret Pinkevitch

Williams Honors College, Honors Research Projects

G protein coupled receptors (GPCRs) are membrane proteins with many functional roles. This family of receptors is expansive, with several subfamilies. Here, a member of subfamily A, rhodopsin (Rho) is studied. Mutations in rhodopsin have been linked to the disease retinitis pigmentosa (RP). Some of these mutations have been found to disrupt the dimerization/oligomerization of Rho at the TM4/TM5 proposed dimerization interface, causing pathogenesis of RP. In this study, two mutations of transmembrane helix 5 were investigated for changes in dimerization and stability, cell trafficking, and signaling. It was found that the V209M and F220C mutations did not result in …

Detection Of Semi-Polar Vitamins And Nutrients In Biological Samples, Jennifer Janovick

Williams Honors College, Honors Research Projects

Metabolomics is used to examine metabolite fluctuations in biological systems and enables the diagnosis of metabolic disorders and the identification of new therapeutic targets.¹ Liquid chromatography-mass spectrometry (LC-MS) was utilized to develop a new method using a T3 column to separate small molecule nutrients and vitamins in a tissue sample. Initial tests of column performance used standard solutions; the amount of metabolites identified and the elution profile of these metabolites was examined. Both the standard solution and sample of brain tissue were tested with the final method developed. Six metabolites were identified in the standard and 31 were identified …

Quantifying Cortical And Cancellous Bone Volume: A Computed Tomography Approach, Hannah Rutkowski

Williams Honors College, Honors Research Projects

Human identification primarily uses long bones of the body such as femora and tibiae which have a high cortical bone amount, these are thought to contain the highest amount of DNA. However, current research shows this is not the case, cancellous bone could contain more DNA in the porous spaces than dense cortical bone. This study aims to measure the variation in the amount of cortical and cancellous bone taken from sampling sites of seven individuals from ten different skeletal elements: femur, tibia, middle rib, calcaneus, first cuneiform, patella, third metacarpal, third metatarsal, first distal phalanx, and cervical vertebra. This …

The Effects Of Aqueous Nitrate On Freshwater Crayfish, Erik Hartman

Williams Honors College, Honors Research Projects

Globalization and a rising need for greater production from the agricultural system have increased the use of fertilizer by farms across the entire country. The purpose of this experiment was to observe the effects of aqueous nitrate (commonly found in many fertilizers) on the metabolic rate and hemolymph lactate concentration of freshwater crayfish. After exposure, there was no observed change in metabolic rate, but there was a significant decrease in hemolymph lactate concentration in the groups exposed to nitrate.

Sporadic Appearance Of Paralytic Spongiform Neurodegeneration In A Colony Of Inbred Mice Is Associated With Cns Retrovirus Expression, Magdalene Durbak

Williams Honors College, Honors Research Projects

Abstract

Exogenous and endogenous retroviruses (RV) have been known to induce vacuolar central nervous system neuropathology. Two mice which were juvenile inbred Rocky Mountain White (IRW), showed signs of spontaneous degenerate motor neuron disease. Signs included wasting, unkempt fur, adduction reflex upon tail elevation, kyphosis, and stilted gait by postnatal day 18 (P18). Brains and spinal cords were examined with H&E staining and stained with a broadly reactive retrovirus antibody. When compared to brains of mice with normal vacuolar pathology as well as mice infected with prototypic non-neurovirulent RV, Fr57E and prototypic neurovirulent RV FrCasE, the subject showed less severity …

Varying Water Stress In Mimulus Ringens, Sara Stiles

Williams Honors College, Honors Research Projects

A wetland plant’s ability to tolerate flooding is important to determining where that plant can grow. Previous studies have shown the optimal flood tolerance of Mimulus ringens is between -2cm and -6cm (Fraser & Karnezis, 2005). This experiment expands on these previous experiments by testing variation in water levels instead of maintaining the water levels at one height throughout the experiment. The hypothesis of the experiment is that Mimulus ringens that have variation in water levels will show signs of better growth than plants with a constant water level. Contrary to predictions, the results showed the final height, number of …

Altering Oligomerization Of Epha2 Via Mutations In The Intracellular Domain, Ryan W. Lingerak

Williams Honors College, Honors Research Projects

Eph receptor tyrosine kinases (RTKs) are activated by membrane-bound ligands called ephrins. Eph RTKs are divided into two subclasses, each activated by a specific classes of the ligand ephrin. The overexpression of Eph receptors is correlated to cancer cell metastasis in several different types of cancers. Studies with the EphA2 extracellular domain (ECD) and ephrinA1 ligand have shown that upon binding of ephrin to the receptor, EphA2 undergoes increased oligomerization and activation. This indicates that oligomerization is intimately connected to kinase activity. High resolution crystal structures of the EphA2 ECD have revealed some details of these ligand bound oligomers, as …

Functionalizing Titanium Implants With Bioactive Peptides To Increase Osseointegration, Neil Bernard

Williams Honors College, Honors Research Projects

Orthopedic surgeries have continued to increase, but the material of choice remains constant: titanium. Titanium has become the benchmark implant material due to its durability and non-immunogenic properties. However, while high rates of success are correlated with joint replacement surgery, certain patients with predisposed diseases such as diabetes or arthritis may have increased complications.¹ This can be due to lack of osseointegration due to decreased bone formation and mineralization,² which will lead to increased rates of infection or aseptic loosening of the implant from the bone. ³ Current methods to alleviate these complications include invasive revisional surgeries, which …

Platelet Rich Plasma Treatment Application For Multiple Purposes, Eric M. Bendel

Williams Honors College, Honors Research Projects

Platelet Rich Plasma (PRP) treatments, a relatively new practice, are used for a variety of medical and clinical treatments. More recently, an increasing number of case studies and research has focused on the efficacy and use of PRP to treat injuries, and potential cosmetic benefits. This article is a compilation of numerous case studies regarding diverse uses of PRP in order to better understand the uses as well as efficacy of the treatment. The applications include: atrophic acne scarring³, orthopedic care², Hepple stage V osteochondral lesion of the talus⁵, chronic non-healing ulcers⁶, …

Preserved Consciousness In The Absence Of A Cerebral Cortex, The Legal And Ethical Implications Of Redefining Consciousness And Its Neural Correlates: A Case For A Subcortical System Generating Affective Consciousness, Shadia Kawkabani

Williams Honors College, Honors Research Projects

Historically, the scientific and medical communities have taken a corticocentric view on consciousness, emphasizing the need for a cortex in producing the conscious experience. The preserved consciousness observed in hydranencephalic children and decorticated rats suggests that some form of consciousness may be produced by a subcortical network. The brainstem, a phylogenetically ancient and conserved brain structure, could serve as the major integrative machinery to produce this form of consciousness, which is called affective consciousness—the evolutionary antecedent to the reflective consciousness that allows humans to reflect on their experiences. The functional convergence of the brainstem with the amygdala, motor system, and …

A Correlation Study Of The Nest Incubation Period And Hatchling Development And Success Rate For Olive Ridley Sea Turtles (Lepidocheyls Olivacea) Turtle Conservation: Playa Junquillal, Costa Rica, Natalie O'Brien

Williams Honors College, Honors Research Projects

Listed as vulnerable by the International Union for the Conservation of Nature (IUCN) the Olive Ridley sea turtle contributes to the stability of an ecosystem by transferring nutrients and energy to both the terrestrial and oceanic environment [1]. Costa Rica holds some of the most prominent nesting beaches for Olive Ridley sea turtles in the World. Collection and relocation of surveyed nests to monitored hatcheries increases the success rate significantly [2]. Realistically, nest and hatchling protection against land-based threats such as poaching, predation and beach erosion is a practical and accessible conservation measure. Facilitated by access to the sea turtle …

Preliminary Investigations Into Endospanin Expression In Zebrafish, Brittany Gray

Williams Honors College, Honors Research Projects

This study investigated endospanin expression in zebrafish by performing western blots to identify which tissues expressed endospanin. A feeding study was also conducted to see if endospanin expression varied in fed versus unfed fish. After tissues were dissected, protein assays were performed on homogenized tissues and then gels were run using gel electrophoresis. The primary antibody rabbit anti-endospanin peptide was used on the finished blots. Chemiluminescence was then used to visualize the blots using GeneSnap software. Endospanin expression was evident in heart, liver, and ovary tissues. The feeding study showed that endospanin expression in liver tissue was not significantly different …

A Chronology Of Diet And Habitat Use In The Florida Manatee (Trichechus Manatus Latirostris): Insights From Stable Isotope Analysis, Malia Smith

Williams Honors College, Honors Research Projects

The Florida Manatee (Trichechus manatus latirostris) is a threatened species that primarily feeds on marine and estuarine seagrasses. Over the past several years, the seagrass coverage in the Indian River Lagoon has varied due to human influence and algal blooms. This led to an unusual number of manatees dying and potentially, to remaining manatees shifting their diets. It can be difficult to decipher why or how diets shift, however, creating a timeline using stable isotopes can help address this challenge. Red blood cells from live Florida Manatees were analyzed for stable carbon, nitrogen, and sulfur isotope ratios. All …

Photosynthetic Activity Under Low Light, Danijela Lonco

Williams Honors College, Honors Research Projects

Are bacteria capable of undergoing oxygenic photosynthesis under low-light conditions inside of caves? Inside of an Australian cave, Chlorophyll f was recently discovered. This chlorophyll pigment was found to demonstrate the capability for photosynthesis, suggesting that Chlorophyll f extends the spectrum of usable light needed for oxygenic photosynthesis (Lars, 2015). Due to the findings in this study, a team went to Carlsbad Caverns to see if there were any bacteria capable of undergoing oxygenic photosynthesis under low light.