Forensic Science and Technology Commons^™

Illegal Gambling And Its Operation Via The Darknet And Bitcoin: An Application Of Routine Activity Theory, Sinyong Choi, Kyung-Shick Choi, Yesim Sungu-Eryilmaz, Hee-Kyung Park

International Journal of Cybersecurity Intelligence & Cybercrime

The Darknet and Bitcoins have been widely utilized by those who wish to anonymously perform illegal activities in cyberspace. Restricted in many countries, gambling websites utilize Bitcoin payments that allow users to freely engage in illegal gambling activities with the absence of a formal capable guardian. Despite the urgency and limited knowledge available to law enforcement regarding this issue, few empirical studies have focused on illegal gambling websites. The current study attempts to examine the characteristics and operations of online gambling websites on both the Darknet and Surface Web, which allow Bitcoin payments. The findings suggest that both websites on …

An Empirical Study To Determine The Role Of File-System In Modification Of Hash Value, Kumarshankar Raychaudhuri, M. George Christopher

International Journal of Cybersecurity Intelligence & Cybercrime

In digital forensics, maintaining the integrity of digital exhibits is an essential aspect of the entire investigation and examination process, which is established using the technique of hashing. Lack of knowledge, while handling digital exhibits, might lead to unintentional alteration of computed hash, rendering the exhibit unacceptable in the court of Law. The hash value of a physical drive does not solely depend upon the data files present in it but also its file-system. Therefore, any change to the file-system might result in the change of the disk hash, even when the data files within it remain untouched. In this …

A Reverse Digital Divide: Comparing Information Security Behaviors Of Generation Y And Generation Z Adults, Scott M. Debb, Daniel R. Schaffer, Darlene G. Colson

International Journal of Cybersecurity Intelligence & Cybercrime

How individuals conceptualize their accountability related to digital technology. There may also be age-based vulnerabilities resulting from personal perceptions about the importance of engaging in best-practices. However, age may not be as critical as experience when it comes to implementation of these behaviors. Using the Cybersecurity Behaviors subscale of the Online Security Behaviors and Beliefs Questionnaire (OSBBQ), this study compared the self-reported cybersecurity attitudes and behaviors across college-aged individuals from Generation Y and Generation Z. Data were derived from a convenience sample of predominantly African-American and Caucasian respondents (N=593) recruited from two public universities in Virginia, USA. Four of the …

Book Review: The Cyber Risk Handbook By Domenic Antonucci, Stanley Mierzwa

International Journal of Cybersecurity Intelligence & Cybercrime

No abstract provided.

Investigating The Potential And Pitfalls Of Ev-Encapsulated Micrornas As Circulating Biomarkers Of Breast Cancer, Brian M. Moloney, Katie E. Gilligan, Doireann P. Joyce, Clodagh P. O'Neill, Killian P. O'Brien, Sonja Khan, Claire L. Glynn, Ronan M. Waldron, Ciarán M. Maguire, Emma Holian, Erin Naughton, Mohamed Elhadi, Andrea B. Grealish, Carmel Malone, Emma Mcdermott, Peter Dockery, Thomas Ritter, Adriele Prina-Mello, Michael J. Kerin, Róisín M. Dwyer

Forensic Science Publications

Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship …

Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn

Forensic Science Publications

Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher’s attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications …

The Costs And Benefits Of Forensics, Brandon L. Garrett

Faculty Scholarship

Supreme Court Justice Louis Brandeis famously wrote that states can be laboratories for experimentation in law and policy. Disappointingly, however, the actual laboratories that states and local governments run are not a home for experimentation. We do not have adequate information about either the costs or the benefits of forensic testing or allocation of resources. Increased spending and expansion of crime laboratories has perversely accompanied growing backlogs. Poor quality control has resulted in a series of audits and even closures of crime laboratories. In response to these problems, however, some laboratories and some entire states have developed new approaches toward …

Justice In Forensic Algorithms, Brandon L. Garrett

Faculty Scholarship

No abstract provided.

Postmortem Interval Estimation Based On Eukaryotic Community Associated With Soil Under Decomposing Porcine Remains, Amanda M. Haase

Master of Science in Forensic Science Directed Research Projects

Recent studies have shown that bacteria associated with soils under a carcass change significantly both temporally and spatially. These studies were done in both laboratory and field conditions, but with limited replications. In addition, limited information exists on temporal and spatial changes in eukaryotic community structure associated with soil under decomposing remains. This study was designed to fill in these gaps and expand on previous studies to improve postmortem interval (PMI) estimation techniques as well as to identify cadaver decomposition islands (CDI) based on eukaryotic community structure associated with soil under vertebrate remains. To accomplish this goal, soil samples were …

From The Legal Literature: Automating Police, Francesca Laguardia

Department of Justice Studies Faculty Scholarship and Creative Works

No abstract provided.

Statistical Assessment Of The Significance Of Fracture Fits In Trace Evidence, Evie K. Brooks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fracture fits are often regarded as the highest degree of association of trace materials due to the common belief that inherently random fracturing events produce individualizing patterns. Often referred to as physical matches, fracture matches, or physical fits, these assessments consist of the realignment of two or more items with distinctive features and edge morphologies to demonstrate they were once part of the same object. Separated materials may provide a valuable link between items, individuals, or locations in forensic casework in a variety of criminal situations. Physical fit examinations require the use of the examiner’s judgment, which rarely can be …

Detection Of Bacterial Lipopolysaccharides Of Escherichia Coli By Direct Analysis In Real Time/Time-Of-Flight Mass Spectrometry, Dakotah M. Biller

Master of Science in Forensic Science Directed Research Projects

Lipopolysaccharides (LPS) are a major component of the outer membrane in Gram-negative bacteria species and are responsible for toxicity caused by organisms such as Escherichia coli (E.coli). Conventional methods for characterizing LPS utilize mass spectrometry, particularly matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and liquid chromatography mass spectrometry (LC-MS). Although these are successful in analyzing intact LPS molecules, they can be time-consuming. Other mass spectrometry techniques, such as direct analysis in real time/time-of-flight mass spectrometry (DART-TOF-MS) could also be helpful in the rapid analysis of LPS molecules without sample extraction procedures. This research focuses on the application …

The Utilization Of Sex Hormone Antibodies For Screening And Separation Of Trace Biological Mixtures, Kristin N. Jones

Master of Science in Forensic Science Directed Research Projects

Touch or trace evidence consists of epidermal cells deposited by contact with items such as handled objects, touched surfaces, or worn clothes. This type of evidence has surpassed most other sample types submitted to forensic labs and typically consists of low quantities of DNA and multiple contributors. In this study epithelial skin cells, i.e., “touch/trace evidence,” were used as they are estimated to constitute approximately half of the casework evidence items submitted for DNA analysis. For the optimization of antibody staining, male and female skin epithelial samples from donors were incubated and hybridized with antibodies of various concentrations of Alexa …

Development Of An Untargeted Gas Chromatography-Mass Spectrometry (Gc/Ms) Method For The Detection Of Drugs In Wastewater, Samuel A. Miller

Master of Science in Forensic Science Directed Research Projects

Monitoring current illicit drug trends and consumption rates of pharmaceuticals using a non-invasive collection technique is needed to address the present drug use and the growing drug epidemic. Reliance on self-reporting drug use surveys is not always a practical measure of illicit drug use. Wastewater analysis has been used globally as a targeted method for monitoring the consumption of specific illicit drugs. Current existing analytical techniques for wastewater analysis focus on the use of targeted liquid chromatography-mass spectrometry (LC-MS) based techniques. Few gas chromatography (GC) procedures exist for wastewater analysis, and those that do concentrate their methods on a single …

Development Of A Quantitative Pcr (Qpcr) Based Method For Studying Temporal Dna Degradation In Waterlogged Bone, Isis Thornton

Master of Science in Forensic Science Directed Research Projects

Human activities are often centered around the presence of water, thus it is not surprising that there are many water-related human deaths. Accumulated degree days (ADD), and other aquatic variables may affect DNA retrieval from waterlogged bone. Calcium and collagen in bone can inhibit the PCR necessary to produce an STR profile; the current solution is a time-consuming organic extraction. While there are examples of research on DNA degradation in terrestrial bone over time, there has been little work done on submerged bone samples and they are usually limited to case studies. The major aim of this study was to …

Expanded Testing Of An Integrated Qpcr Mixture Screening Assay Using High Resolution Melt Curve Analysis And Support Vector Machine Modeling, Andrea L. Williams

Master of Science in Forensic Science Directed Research Projects

In the conventional forensic DNA workflow, the number of contributors in a sample is unknown until the final step of STR analysis. We propose a high-resolution melt curve (HRM) mixture screening assay, which uses support vector machine (SVM) modeling of melt morphologies of D5S818 and D18S51 amplicons integrated into a common qPCR-based human DNA quantification kit, to differentiate between single-source samples (and their genotypes) and mixtures at an earlier stage in the DNA workflow (quantification). Previously, using data generated from whole melt curves, 87.5% of single-source samples and 100% of 1:1 mixture samples (2 contributors) classified accurately.

In this study, …

The Effect Of Time And Temperature On The Quality Of Latent Fingerprints On Incandescent Lightbulbs, Varying Donors Age And Sex, Kinaysha M. Collazo Maldonado

Master of Science in Forensic Science Directed Research Projects

Fingerprints are used as a means of identification, but there are no established methodologies to determine time since deposition of latent fingerprints by visual means alone. This research considered the influence of age and sex on the quality of recovered latent prints from lit and unlit lightbulbs from 1 to 10 days, using accumulated degree hours (ADH) to account for both heat and time simultaneously. Two male and two female donors (one of each aged40 years) were used. A thermal imaging camera was used to monitor the lightbulbs top and middle regions, which were significantly different (p≤0.05) for the experimental …

Effective Laundering Conditions For Ignitable Liquid Decontamination Of Fire Scene Clothing Used By The Bureau Of Alcohol, Tobacco, Firearms, And Explosives, Rebecca K. Whitney

Master of Science in Forensic Science Directed Research Projects

Ignitable liquids such as gasoline and diesel fuel are common accelerants encountered by Certified Fire Investigators (CFIs). These ignitable liquids can be identified by gas chromatography-mass spectroscopy; the presence of particular peaks in appropriate abundances and patterns indicates the presence of compounds found in ignitable liquids. The Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF) issues clothing to its CFIs for wear during fire scene investigation. Previous research has identified a method to decontaminate the tools used by CFIs, but no explicit protocol to decontaminate scene clothing has been determined. This study investigated the use of Tide Ultra Stain Release, …

Discrimination Of Colorless Fibers By Uv-Vis Microspectrophotometry And Microspectrofluorimetry, Kialani Killinger

Master of Science in Forensic Science Directed Research Projects

Fiber evidence is frequently encountered in forensic casework, and part of a typical fiber analytical scheme involves the detailed study of the color and other physical properties, optical properties, and chemical composition of the fibers in question. Microspectrophotometry (MSP) is commonly used to provide objective color measurements of fibers, eliminating subjectivity that may be present in visual color examinations. MSP can produce color measurements over the ultraviolet (UV) and visible (VIS) regions of the electromagnetic spectrum, as well as emission spectra from fluorescence measurements. In this research, colorless fibers were analyzed by MSP, using both transmission measurements in the UV-VIS …

Analysis Of Pellet Patterns And Gsr Produced From Judge Taurus Revolvers, Donald Poon

Master of Science in Forensic Science Directed Research Projects

The Taurus Judge is a five-shot revolver designed to fire both 410 Bore shotshells and caliber 45 (Long) Colt cartridges. It is marketed as a self-defense firearm by Taurus. As of 2010, the Taurus Judge was “quickly becoming a popular personal defense firearm”.¹ Although it has become somewhat popular, the Taurus Judge and its various models have not had many distance determination studies completed and there is still a dearth of information on how these handguns compare with shotguns when looking at range of fire determinations. This study focused on: how barrel length affected pellet spread patterns produced by …