Veterinary Preventive Medicine, Epidemiology, and Public Health Commons^™

A Comparison Of Cost And Quality Of Three Methods For Estimating Density For Wild Pig (Sus Scrofa), Amy J. Davis, David A. Keiter, Elizabeth M. Kierepka, C. Slootmaker, Antoinette J. Piaggio, James C. Beasley, K. M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

A critical element in effective wildlife management is monitoring the status of wildlife populations; however, resources to monitor wildlife populations are typically limited. We compared cost effectiveness of three common population estimation methods (i.e. non-invasive DNA sampling, camera sampling, and sampling from trapping) by applying them to wild pigs (Sus scrofa) across three habitats in South Carolina, U.S.A where they are invasive. We used mark-recapture analyses for fecal DNA sampling data, spatially-explicit capture-recapture analyses for camera sampling data, and a removal analysis for removal sampling from trap data. Density estimates were similar across methods. Camera sampling was the least expensive, …

Technical Limitations Associated With Molecular Barcoding Of Arthropod Bloodmeals Taken From North American Deer Species, Erin M. Borland, Daniel A. Hartman, Matthew W. Hopken, Antoinette J. Piaggio, Rebekah C. Kading

United States Department of Agriculture Wildlife Services: Staff Publications

Accurate species-level identification of the source of arthropod bloodmeals is important for deciphering blood feeding patterns of field-collected specimens. Cytochrome c oxidase I (COI) mitochondrial gene sequencing has been used for this purpose; however, species resolution can be difficult to obtain from certain vertebrate genera, including Odocoileus. Sanger sequencing of mitochondrial genes was employed to identify the bloodmeal source of wild-caught mosquitoes trapped in Greeley, Colorado. Initial sequencing of the COI gene of mitochondrial DNA in bloodmeals was inadequate for species-level resolution of bloodmeals from deer in the genus Odocoileus, with current databases returning low fidelity matches to …

Additive And Non-Additive Responses Of Seedlings To Simulated Herbivory And Drought, Kasey Barton, Aaron B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Drought is a global threat, increasing in severity and frequency throughout tropical ecosystems. Although plants often face drought in conjunction with biotic stressors, such as herbivory or disease, experimental studies infrequently test the simultaneous effects of drought and biotic stress. Because multiple simultaneous stressors may have non-additive and complex effects on plant performance, it is difficult to predict plant responses to multiple threats from research examining one stress at a time. Using an experimental approach in the greenhouse, we investigated potential non-additivity in seedling growth and survival to simulated drought and herbivory across a phylogenetically diverse pool of ten Hawaiian …

The Changing Triad Of Plague In Uganda: Invasive Black Rats (Rattus Rattus), Indigenous Small Mammals, And Their Fleas, Russell E. Enscore, Nackson Babi, Gerald Amatre, Linda Atiku, Rebecca J. Eisen, Kim M. Pepin, Rommelle Vera-Tudela, Christopher Sexton, Kenneth L. Gage

United States Department of Agriculture Wildlife Services: Staff Publications

Rattus rattus was first reported from the West Nile Region of Uganda in 1961, an event that preceded the appearance of the first documented human plague outbreak in 1970. We investigated how invasive R. rattus and native small mammal populations, as well as their fleas, have changed in recent decades. Over an 18-month period, a total of 2,959 small mammals were captured, sampled, and examined for fleas, resulting in the identification of 20 small mammal taxa that were hosts to 5,109 fleas (nine species). Over three-fourths (75.8%) of captured mammals belonged to four taxa: R. rattus, which predominated inside huts, …

Rnai: Applications In Vertebrate Pest Management, Katherine Horak

United States Department of Agriculture Wildlife Services: Staff Publications

Sequence-directed inhibition of protein synthesis by RNAi has potential as a means to control pest wildlife. Species specific by design, RNAi reduces impacts on nontarget species and the environment. Additional research advancing the field of RNAi-based management of vertebrate pest wildlife is timely.

Despite the potential diverse applications of RNAi technology in vertebrates (fertility control, invasive species eradication, and pest species control to protect human health and agriculture), little progress has been made in applying RNAi to these classes of animals. A single proof-of-concept study using RNAi to control sea lampreys combined with recent advances in RNAi delivery have opened …

Avian Influenza A Virus Associations In Wild, Terrestrial Mammals: A Review Of Potential Synanthropic Vectors To Poultry Facilities, J. Jeffrey Root, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

The potential role of wild mammals in the epidemiology of influenza A viruses (IAVs) at the farm-side level has gained increasing consideration over the past two decades. In some instances, select mammals may be more likely to visit riparian areas (both close and distant to farms) as well as poultry farms, as compared to traditional reservoir hosts, such as waterfowl. Of significance, many mammalian species can successfully replicate and shed multiple avian IAVs to high titers without prior virus adaptation and often can shed virus in greater quantities than synanthropic avian species. Within this review, we summarize and discuss the …

Improving The Accessibility And Transferability Of Machine Learning Algorithms For Identification Of Animals In Camera Trap Images: Mlwic2, Michael A. Tabak, Mohammad S. Norouzzadeh, David W. Wolfson, Erica J. Newton, Raoul K. Boughton, Jacob S. Ivan, Eric Odell, Eric S. Newkirk, Reesa Y. Conrey, Jennifer Stenglein, Fabiola Iannarilli, John Erb, Ryan K. Brook, Amy J. Davis, Jesse Lewis, Daniel P. Walsh, James C. Beasley, Kurt C. Vercauteren, Jeff Clune, Ryan S. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Motion-activated wildlife cameras (or “camera traps”) are frequently used to remotely and noninvasively observe animals. The vast number of images collected from camera trap projects has prompted some biologists to employ machine learning algorithms to automatically recognize species in these images, or at least filter-out images that do not contain animals. These approaches are often limited by model transferability, as a model trained to recognize species from one location might not work as well for the same species in different locations. Furthermore, these methods often require advanced computational skills, making them inaccessible to many biologists. We used 3 million camera …

Tissue Tropisms Of Avian Influenza A Viruses Affect Their Spillovers From Wild Birds To Pigs, Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Wild aquatic birds maintain a large, genetically diverse pool of influenza A viruses (IAVs), which can be transmitted to lower mammals and, ultimately, humans. Through phenotypic analyses of viral replication efficiency, only a small set of avian IAVs were found to replicate well in epithelial cells of the swine upper respiratory tract, and these viruses were shown to infect and cause virus shedding in pigs. Such a phenotypic trait of the viral replication efficiency appears to emerge randomly and is distributed among IAVs across multiple avian species and geographic and temporal orders. It is not determined by receptor binding preference …

Using Bioenergetics And Radar-Derived Bird Abundance To Assess The Impact Of A Blackbird Roost On Seasonal Sunflower Damage, Bonnie A. Clark, Page E. Klug, Phillip M. Stepanian, Jeffrey F. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Methods aimed at reducing avian damage to agricultural crops are routinely implemented in situations where efficacy can be assessed by quantifying blackbird (Icteridae) abundance relative to environmental variables and extrapolating to ensuing crop damage. Concomitantly, Weather Surveillance Radar (WSR) data may have potential to enhance crop damage mitigation through improved monitoring of nuisance wildlife populations. We used WSR to derive daily abundance estimates of blackbirds at a fall roost in North Dakota, USA from 2012 to 2019. We integrated these estimates with previously developed bioenergeticseconomic models to estimate local sunflower (Helianthus annuus) damage. The greatest losses usually occurred during …

Historic And Contemporary Use Of Catfish Aquaculture By Piscivorous Birds In The Mississippi Delta, Paul C. Burr, Jimmy L. Avery, Garrett M. Street, Bronson K. Strickland, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Piscivorous birds are the primary source of catfish (Ictalurus spp.) depredation at aquaculture facilities in northwestern Mississippi. Of particular concern is the Double-crested Cormorant (Phalacrocorax auritus), which can cost aquaculture producers millions of dollars annually through the depredation of cultured fish. Historical research conducted in the early 2000s estimated cormorant use of aquaculture ponds in the region, but aquaculture area has decreased by more than 70% since those estimates were made. With less aquaculture available, we predicted cormorant densities on aquaculture would be greater today than historically. Applying a similar methodology as in historical studies, we used …

Invasive Species Control And Resolution Of Wildlife Damage Conflicts: A Framework For Chemical And Genetically Based Management Methods, Larry Clark, John Eisemann, John Godwin, Katherine Horak, Kevin Oh, Jeanette R. O'Hare, Antoinette J. Piaggio, Kim M. Pepin, Emily W. Ruell

United States Department of Agriculture Wildlife Services: Staff Publications

Vertebrate wildlife damage management relates to developing and employing methods to mitigate against damage caused by wildlife in the areas of food production, property damage, and animal or human health and safety. Of the many management tools available, chemical methods (e.g., toxicants) draw the most attention owing to issues related to environmental burden, species specificity, and humaneness. Research and development focusing on RNA interference and gene drives may be able to address the technical aspects of performance goals. However, there remain many questions about regulation, environmental risk, and societal acceptance for these emerging biological technologies. Here we focus on the …

Repellent Surface Applications For Pest Birds, Shelagh T. Deliberto, James C. Carlson, Hailey E. Mclean, Caroline S. Olson, Scott J. Werner

United States Department of Agriculture Wildlife Services: Staff Publications

Common pest birds in the United States include the non-native European starling (Sturnus vulgaris), house sparrow (Passer domesticus), and the pigeon (Columba livia domestica), as well as native birds including Canada geese (Branta canadensis) and gull species (Laridae). Large concentrations of pest birds can create human health hazards and monetary losses due to consumption of crops, depredation, and fecal contamination and accumulation. Fecal contamination hazards include the potential spread of zoonotic diseases including antimicrobial-resistant zoonoses and human injury due to the accumulation of fecal material on walking surfaces. Additionally, fecal accumulation causes …

Black Vulture Conflict And Management In The United States: Damage Trends, Management Overview, And Research Needs, Bryan M. Kluever, Morgan Pfeiffer, Scott C. Barras, Brett Dunlap, Lee A. Humberg

United States Department of Agriculture Wildlife Services: Staff Publications

Contrary to rapid declines of many vulture (Accipitridae, Cathartidea) species worldwide, black vulture (Coragyps atratus) populations are increasing and expanding their range in North America. Vultures exhibit complex behaviors and can adapt to any human-dominated landscape or land use. These traits, combined with population growth and range expansion, have contributed to increased human–vulture conflicts. Our goal was to summarize the current status and trends in human–black vulture conflicts (hereafter human– vulture conflicts), review available management strategies, identify knowledge gaps, and provide recommendations to enhance management and understanding of this species and the associated conflicts. We found human–vulture conflicts …

Review Of Ospreys: The Revival Of A Global Raptor, By Alan F. Poole. 2019., Lisa Fields, Brian E. Washburn

United States Department of Agriculture Wildlife Services: Staff Publications

For the past three decades, Alan Poole’s (1989) Ospreys: A Natural and Unnatural History has provided a solid foundation for those seeking a primer on the basic biology and ecology of Ospreys (Pandion haliaetus). Poole’s summary of knowledge about Ospreys was written at a time when these amazing birds were recovering from their DDTinduced population crash, but this important book has long been out of print. In the intervening decades, much has been learned regarding Ospreys. In his new book, Ospreys: The Revival of a Global Raptor, Poole guides the reader through a tour of Ospreys’ worldwide distribution and population …

Population Increases Of Large Birds In North America Pose Challenges For Aviation Safety, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

There is a strong correlation between bird mass and the likelihood of aircraft damage during a bird–aircraft collision. Thus, the U.S. Federal Aviation Administration (FAA) has established airworthiness standards related to bird mass for engines, airframes, and windshields. Most standards use large (1.8 kg) and medium (1.1 kg) birds as benchmarks (the empennage and certain large turbofan engines use a 3.6-kg bird). There are 20 large (≥1.8 kg) and 16 medium (1.1–1.7 kg) bird species in North America with ≥20 strikes reported for civil aircraft (FAA National Wildlife Strike Database), 1990 to 2018. I analyzed the population changes of these …

One Welfare, The Role Of Health Professionals, And Climate Change, Anne Fawcett

Animal Sentience

Wiebers & Feigin argue that the COVID-19 crisis is a call for humanity to rethink our relationships with animals and the environment. This One Welfare approach has implications for the role of health care professionals and demands that we address climate change.

Mapping Of Diabetes Susceptibility Loci In A Domestic Cat Breed With An Unusually High Incidence Of Diabetes Mellitus, Lois Balmer, Caroline Ann O’Leary, Marilyn Menotti-Raymond, Victor David, Stephen O’Brien, Belinda Penglis, Sher Hendrickson, Mia Reeves-Johnson, Susan Gottlieb, Linda Fleeman, Dianne Vankan, Jacquie Rand, Grant Morahan

Research outputs 2014 to 2021

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Genetic variants that are associated with susceptibility to type 2 diabetes (T2D) are important for identification of individuals at risk and can provide insights into the molecular basis of disease. Analysis of T2D in domestic animals provides both the opportunity to improve veterinary management and breeding programs as well as to identify novel T2D risk genes. Australian-bred Burmese (ABB) cats have a 4-fold increased incidence of type 2 diabetes (T2D) compared to Burmese cats bred in the United States. This is likely attributable to a genetic founder effect. We investigated this …

A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler

United States Department of Agriculture Wildlife Services: Staff Publications

Antimicrobial resistance is a threat to agricultural production and public health. In this proof-of-concept study, we investigated predicting antimicrobial sensitive/resistant (S/R) phenotypes and host sources of Escherichia coli (n = 128) based on differential fatty acid abundance. Myristic (14:0), pentadecanoic acid (15:0), palmitic (16:0), elaidic (18:19) and steric acid (18:0) were significantly different (α = 0.05) using a two-way ANOVA for predicting nalidixic acid, ciprofloxacin, aztreonam, cefatoxime, and ceftazidime S/R phenotypes. Additionally, analyses of palmitoleic (16:1), palmitic acid (16:0), methyl palmitate (i-17:0), and cis-9,10-methyleneoctadecanoic acid (19:0Δ) showed these markers were significantly different (α = 0.05) between isolates obtained from cattle …

Male Burmese Pythons Follow Female Scent Trails And Show Sex-Specific Behaviors, Shannon A. Richard, Eric A. Tillman, John S. Humphrey, Michael L. Avery, M. Rockwell Parker

United States Department of Agriculture Wildlife Services: Staff Publications

Animals communicate with potential mates using species-specific signals, and pheromones are powerful sexual signals that modify conspecific behavior to facilitate mate location. Among the vertebrates, snakes are especially adept in mate searching via chemical trailing, which is particularly relevant given that many snake species are invasive outside their native ranges. Chemical signals used in mate choice are, thus, potentially valuable tools for management of invasive snake species. The Burmese python (Python bivittatus) is an invasive snake in the Florida Everglades where it is negatively impacting native fauna. In this study, we sought to: (i) determine if males can follow conspecific …

Variation In Host Home Range Size Decreases Rabies Vaccination Effectiveness By Increasing The Spatial Spread Of Rabies Virus, Katherine M. Mcclure, Amy T. Gilbert, Richard B. Chipman, Erin E. Rees, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Animal movement influences the spatial spread of directly transmitted wildlife disease through host-host contact structure. Wildlife disease hosts vary in home range- associated foraging and social behaviours, which may increase the spread and intensity of disease outbreaks. The consequences of variation in host home range movement and space use on wildlife disease dynamics are poorly understood, but could help to predict disease spread and determine more effective disease management strategies.
2. We developed a spatially explicit individual-based model to examine the effect of spatiotemporal variation in host home range size on the spatial spread rate, persistence and incidence of rabies virus …