Veterinary Preventive Medicine, Epidemiology, and Public Health Commons^™

Evaluation Of Ankom F58 Filter Bags Compared To Beakers For Analysis Of Neutral Detergent Fiber, Sawyer R. Kappel, Rebecca L. Sjostrand, J. C. Macdonald

Nebraska Beef Cattle Reports

Feed ingredient, feed refusals, duodenal and fecal samples were analyzed to compare two methods of determining neutral detergent fiber. All samples were weighed into Ankom F58 fiber bags and Analyzed using an Ankom 2000 automated fiber Analyzer. Results were then compared to the Van Soest beaker method. The fiber values determined from both methods were within 3.5% of one another, with the beaker method being consistently greater compared to the Ankom method, except for fecal samples. Variability in fiber estimates for ingredients, feed refusals, and feces translated to substantial inconsistency in estimated neutral detergent fiber digestibilities among treatments. It is …

Forage Evaluation Of Crested Wheatgrass, Kelton C. Adair, K. H. Jenkins Pas, Andrea K. Watson, James C. Macdonald

Nebraska Beef Cattle Reports

An experiment evaluated the forage value of crested wheatgrass (CWG) harvested throughout the grazing season near Sidney, Nebraska over a two-year period (2019-2020). The purpose of the evaluation was to determine forage quality and rumen undegradable protein (RUP) content to help producers with supplementation decisions for cattle grazing monoculture CWG pastures. In vitro dry matter disappearance quadratically decreased from 54% in May to 37% in September of 2019, with no changes throughout 2020, averaging 43%. In both years, crude protein (CP) decreased throughout the growing season while rumen undegradable protein (RUP) increased (as % CP). Digestible RUP was less than …

Effects Of Strategic Supplementation On Return To Management And Performance Of Yearling Cattle, Kaylee E. Wheeler, Dean Dustin, Jay Parsons, M. E. Drewnoski, K. H. Jenkins Pas

Nebraska Beef Cattle Reports

A three-year experiment evaluated the effects of two supplementation strategies on yearling cattle performance and producer returns to management. Yearling cattle grazed on crested wheatgrass pastures and were supplemented either throughout the entire grazing season, only during the latter part of the grazing season, or not supplemented at all. The supplemented yearlings received 3.5 lb of dried distillers grains with solubles 6 days/week. On average non-supplemented yearlings had an average daily gain of 1.1 lb/d and providing supplement increased average daily gain by 0.5 lb/d. Yearlings supplemented during the latter part of the season had similar performance to yearlings supplemented …

Timing Of Implant Use In The Backgrounding System, Kelton C. Adair, Kylie M. Butterfield, Zach E. Carlson, Braden C. Troyer, Jiehua Xiong, Mitchell M. Norman, James C. Macdonald, Galen E. Erickson, M. E. Drewnoski, Brandon L. Nuttelman

Nebraska Beef Cattle Reports

A two-year study utilized 240 weaned steers each year in a 3-phase yearling production system: winter backgrounding, summer grazing, and finishing. The objective of the study was to determine the effects of winter rate of gain (LOW or HIGH) and implant strategy during the winter backgrounding and summer grazing periods on compensatory gain, animal performance, and carcass characteristics. Calves received 1 of 3 implant strategies: NONE, SINGLE (Revalor-G during summer grazing), and MULTIPLE (Ralgro during winter backgrounding and Revalor-G during summer grazing). All cattle received a Revalor-XS during the finishing phase. Implant strategy and rate of gain during the winter …

Artificial Insemination Of Beef Heifers With Multi-Sire Sexed Semen, Dempster Christenson, John Maddux, Jordan M. Thomas, R. Funston

Nebraska Beef Cattle Reports

This study observed pregnancy outcomes resulting from artificial insemination of beef heifers with multi-sire sexed semen. It was hypothesized, pregnancy rates resulting from multi-sire sexed semen would be increased compared to pregnancy rates from prior studies using single-sire sexed semen. Producers looking to maximize the proportion of calves of one sex may consider the technique. Ultrasound determined pregnancy rate of heifers given multi-sire sexed semen was 65%, which is 12% greater than the average pregnancy rate reported in previous studies using single-sire sexed semen. After accounting for date of birth however, the adjusted AI conception rate was calculated to range …

Effect Of Increasing Energy 30 Days Prior To Artificial Insemination In Beef Heifers, Landon F. Tadich, Rachel E.S. Rogers, Jim R. Teichert, R. Funston

Nebraska Beef Cattle Reports

A 3-yr study utilized 300 Angus-based, spring-born heifers to evaluate postweaning heifer development systems on gain and reproductive performance. Three groups of heifers were developed over the winter development period either grazing winter range or fed a dry lot diet targeted to 1.5 lb/d of gain in order to achieve 65% of their mature body weight at breeding. Thirty days prior to artificial insemination, heifers grazing winter rand entered the dry lot and were fed this same diet, one group of dry lot heifers remained on this diet, and the other received increased energy in the form of wet corn …

Including Gene Edited Sires In Genetic Evaluations, Leticia P. Sanglard, Garret M. See, Matthew L. Spangler

Nebraska Beef Cattle Reports

A simulation study investigated and provided potential solutions to practical issues that could arise from including gene-edited sires in routine genetic evaluations. Gene-editing is a technique for adding, deleting, or replacing nucleotides in the genome. Editing nucleotides controlling important socioeconomic traits (e.g., growth, carcass, disease susceptibility) is expected to improve rates of genetic gain. However, targeted alterations of the genome can affect the relationship among individuals and, consequently, introduce bias in Expected Progeny Differences. The current study illustrated that, indeed, Expected Progeny Differences for the progeny of edited sires were underestimated. Consequently, these animals would be less likely to be …

Comparison Of Semi-Confined And Pasture-Based August Calving Beef Cow Systems, Hannah F. Speer, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

Limited pasture availability and increased pasture rental rates have generated a need to evaluate alternative cow-calf production systems. The current study compared cow and calf performance in two August-calving cow systems that combined corn residue grazing with 1) perennial forage grazing and hay or 2) summer drylot feeding and fall cover crop grazing. Differences in pregnancy rates between systems within year were not observed; however, the effect of production system on cow body condition and calf body weight at different time points varied across years. Overall, cow and calf performance were not negatively impacted in the drylot/cropland system, suggesting that …

Impact Of Planning Horizon Length On Breeding Objectives And Resulting Selection Decisions, Hunter F. Valasek, Bruce L. Golden, Matthew L. Spangler

Nebraska Beef Cattle Reports

The weighting of traits in a selection index depends on varying circumstances that can difer among beef producers (e.g. economic factors, breeding systems). The determination of planning horizon is an additional variable that can differ among producers that represents the impact of genetic selection decisions over a gradient of time. A web-based economic index construction platform (iGENDEC) was used in the current study to investigate the implications of planning horizon on relative emphasis values of traits within the breeding goal and potential differences in sire selection decisions. General-purpose indexes were created for three breeding systems under six different …

Interspecific Oral Rabies Vaccine Bait Competition In The Southeast United States, Wesley C. Dixon, Jacob E. Hill, Richard Chipman, Amy J. Davis, Amy Gilbert, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

The United States Department of Agriculture’s National Rabies Management Program (NRMP) has coordinated the use of oral rabies vaccination (ORV) to control the spread of raccoon rabies virus variant west of the Appalachian Mountains since 1997. Working with state and local partners, the NRMP deploys ORV baits containing a rabies vaccine, primarily targeting raccoon populations (Procyon lotor). Bait competition between raccoons and non-target species may limit the effectiveness of ORV programs, but the extent of bait competition remains poorly quantified, particularly in the southeastern United States. We placed placebo ORV baits in bottomland hardwood (n = 637 baits) and upland …

Quantifying Residual Feed In A Fence-Line Feedlot Bunk Using Depth Camera Imaging Techniques, Jean Niwenshuti, Galen E. Erickson, Tami M. Brown-Bandl, Eric T. Psota, Yijie Xiong

Nebraska Beef Cattle Reports

Feed bunk management requires intensive labor and relies on manual observation to estimate the amount of residual feed in the bunk. Alternative and innovative technologies were used to estimate the weight of residual feed in a concrete fence-line bunk using a depth camera. Depth cameras capture the distance between the camera and the object in their field of view. This study used a time-of-flight depth camera (Azure Kinect, Microsoft) to estimate the weight of residual feed in a partial fence-line concrete bunk using 11 common feed ingredients. The camera was fastened approximately 3.3 ft above the center of the bunk …

Extending Melengestrol Acetate Treatment From Fourteen To Eighteen Days In Beef Heifers, Dempster Christenson, Jordan M. Thomas, Daniel J. Kelly, R. Funston

Nebraska Beef Cattle Reports

This study compared estrus synchronization, estrus response, and artificial insemination pregnancy rates of beef heifers fed melengestrol acetate the normal 14-day period or extended to an 18-day period using the melengestrol acetate -prostaglandin- heat detect and timed artificial insemination protocol. Therefore, the purpose of this study was to evaluate if extending melengestrol acetate feeding by 4 days increases the number of heifers ovulating, which would result in greater estrus response and pregnancy rate in the whole herd. Early estrus response following prostaglandin administration occurred in heifers who averaged an earlier estrus response after melegestrol acetate withdrawal but extending the melengestrol …

First Bovine Vaccine To Prevent Human Schistosomiasis - A Cluster Randomised Phase 3 Clinical Trial, Allen G. Ross, Donald A. Harn, Delia Chy, Marianette Inobaya, Jerric R. Guevarra, Lisa Shollenberger, Yuesheng Li, Donald P. Mcmanus, Darren J. Gray, Gail M. Williams

Biological Sciences Faculty Publications

Objective

Schistosomiasis is a neglected tropical parasitic disease caused by blood flukes of the genus Schistosoma. Schistosoma japonicum is zoonotic in China, the Philippines, and Indonesia, with bovines acting as major reservoirs of human infection. The primary objective of the trial was to examine the impact of a combination of human mass chemotherapy, snail control through mollusciciding, and SjCTPI bovine vaccination on the rate of human infection.

Methods

A 5-year phase IIIa cluster randomized control trial was conducted among 18 schistosomiasis-endemic villages comprising 18,221 residents in Northern Samar, The Philippines.

Results

Overall, bovine vaccination resulted in a statistically significant decrease …

The Use Of A Habitat Quality Stress Index To Evaluate Stress As An Analog For Proximate Fitness In The American Crow Within A Matrix Of Landcover Characteristics To Assess Its Potential Contribution To Disease Etiologies, Theodore Lee Grabarz

Antioch University Dissertations & Theses

All organisms occur within spatial and temporal environments to maximize proximate fitness (health) and thus life history outcomes. Previous work has examined the temporal and behavioral aspects of proximate fitness on life history outcomes particularly regarding highly perturbed environments (i.e., climate and land use change, resource extraction, agricultural erosion, etc.). My work focuses on the less examined spatial aspect of these perturbed environments. More specifically, this dissertation examines habitat selection and quality as the basis for understanding stress response (negative and positive feedback mechanisms) to environmental stressors within the larger context of regional or gamma (ɣ) biodiversity. Through the lens …

Straight From The Coyote’S Mouth: Genetic Identification Of Prey Through Oral Swabs Of Predators, Julie K. Young, Amanda M. Mast, James A. Walton, Torrey Rodgers, Antionette J. Piaggio, Daniel R. Taylor, Karen E. Mock

United States Department of Agriculture Wildlife Services: Staff Publications

Human-carnivore conflicts often involve the depredation of domestic livestock. These depredation events are rarely observed, yet mitigation typically involves identifying the species or individual involved for removal or relocation. We tested a molecular method to identify individuals involved in depredation events using mouth swabs to determine if prey DNA could be detected, and for how long. We fed mule deer Odocoileus hemionus meat to captive coyotes Canis latrans and swabbed their mouths at five predetermined intervals between 2–72 h after consumption of the deer meat. We assessed two different molecular forensic methods to analyze the saliva swabs: qPCR for species …

Movements And Resource Selection Of Wild Pigs Associated With Growth Stages Of Corn, Bethany A. Friesenhahn, Randall W. Deyoung, Michael J. Cherry, Humberto L. Perotto-Baldivieso, Kurt C. Vercauteren, Nathan P. Snow

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) are one of the most successful invasive species globally and are often implicated in agricultural damage. This damage is expected to increase as ranges of wild pigs expand, impacting the human food supply and increasing costs of food production. Our objective was to evaluate movement behaviors of wild pigs relative to resource availability and landscape features in an agriculture-dominated landscape, with a goal of informing management practices for reducing damage to corn. We monitored hourly movements of adult wild pigs relative to corn crops using GPS collars during the 2019 and 2020 growing seasons (Feb–Sept) in …

Burmese Pythons In Florida: A Synthesis Of Biology, Impacts, And Management Tools, Jacquelyn C. Guzy, Bryan G. Falk, Brian J. Smith, John David Willson, Robert N. Reed, Nicholas G. Aumen, Michael L. Avery, Ian A. Bartoszek, Earl Campbell, Michael S. Cherkiss, Natalie M. Claunch, Andrea F. Currylow, Tylan Dean, Jeremy Dixon, Richard Engeman, Sarah Funck, Rebekah Gibble, Kodiak C. Hengstebeck, John S. Humphrey, Margaret E. Hunter, Jillian M. Josimovich, Jennifer Ketterlin, Michael Kirkland, Frank J. Mazzotti, Robert Mccleery, Melissa A. Miller, Matthew Mccollister, M. Rockwell Parker, Shannon E. Pittman, Michael Rochford, Christina Romagosa, Art Roybal, Ray W. Snow, Mckayla M. Spencer, J. Hardin Waddle, Any A. Yackel Adams, Kristen M. Hart

United States Department of Agriculture Wildlife Services: Staff Publications

Burmese pythons (Python molurus bivittatus) are native to southeastern Asia, however, there is an established invasive population inhabiting much of southern Florida throughout the Greater Everglades Ecosystem. Pythons have severely impacted native species and ecosystems in Florida and represent one of the most intractable invasive-species management issues across the globe. The difficulty stems from a unique combination of inaccessible habitat and the cryptic and resilient nature of pythons that thrive in the subtropical environment of southern Florida, rendering them extremely challenging to detect. Here we provide a comprehensive review and synthesis of the science relevant to managing invasive …

Effects Of Corn Supplementation On Serum And Muscle Microrna Profiles In Horses, Clarissa Carver, Jason E. Bruemmer, Stephen Coleman, Gabriele Landolt, Tanja Hess

United States Department of Agriculture Wildlife Services: Staff Publications

Laminitis associated with equine metabolic syndrome causes significant economic losses in the equine industry. Diets high in non-structural carbohydrates (NSC) have been linked to insulin resistance and laminitis in horses. Nutrigenomic studies analyzing the interaction of diets high in NSCs and gene expression regulating endogenous microRNAs (miRNA) are rare. This study's objectives were to determine whether miRNAs from dietary corn can be detected in equine serum and muscle and its impacts on endogenous miRNA. Twelve mares were blocked by age, body condition score, and weight and assigned to a control (mixed legume grass hay diet) and a mixed legume hay …

Using Drones To Detect And Quantify Wild Pig Damage And Yield Loss In Corn Fields Throughout Plant Growth Stages, Bethany A. Friesenhahn, Lori D. Massey, Randy W. Deyoung, Michael J. Cherry, Justin W. Fischer, Nathan P. Snow, Kurt C. Vercauteren, Humberto L. Perotto‐Baldivieso

United States Department of Agriculture Wildlife Services: Staff Publications

Presently, there are an estimated 6.9 million wild pigs (Sus scrofa) in the U.S., which cause over US$1 billion in damage to agriculture, environmental impacts, and control costs. However, estimates of damage have varied widely, creating a need for standardized monitoring and a method to accurately estimate the economic costs of direct wild pig damage to agriculture. The goal of our study was to integrate remotely sensed imagery from drones and crop harvest data to quantify wild pig damage in corn fields. We used drones with natural color (red, green, blue) cameras to monitor corn fields at different …

Intracellular Diversity Of Wnv Within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns Of Polyinfection, Dalit Talmi-Frank, Alex D. Byas, Reyes Murrieta, James Weger-Lucarelli, Claudia Rückert, Emily N. Gallichotte, Janna A. Yoshimoto, Chris Allen, Angela M. Bosco-Lauth, Barbara Graham, Todd A. Felix, Aaron C. Brault, Gregory D. Ebel

United States Department of Agriculture Wildlife Services: Staff Publications

Arthropod-borne virus (arbovirus) populations exist as mutant swarms that are maintained between arthropods and vertebrates. West Nile virus (WNV) population dynamics are host-dependent. In American crows, purifying selection is weak and population diversity is high compared to American robins, which have 100- to 1000-fold lower viremia. WNV passed in robins leads to fitness gains, whereas that passed in crows does not. Therefore, we tested the hypothesis that high crow viremia allows for higher genetic diversity within individual avian peripheral blood mononuclear cells (PBMCs), reasoning that this could have produced the previously observed hostspecific differences in genetic diversity and fitness. Specifically, …