Veterinary Medicine Commons^™

The Stereological And Morphometrical Investigation Of Pars Lumbalis Of The Spinal Cord Innew Zealand Rabbit, Berna Duman, İftar Gürbüz

Turkish Journal of Veterinary & Animal Sciences

In the study, 12 (6 females, 6 males) healthy adult New Zealand rabbits were used to examine the morphometrical andstereological values. White matter, gray matter and total segment volume of the segments in the pars lumbalis were calculated byCavalieri’s principle. The findings were grouped according to sexand statistically evaluated. As a result, 38% of the length of the spinalcord in rabbits belonged to the pars lumbalis. The morphometric parameters were not statistically different between male and femalerabbits (p > 0.05). The segment with the biggest volume was L5. White matter’s greatest volume was the L4 segment. The volume of graymatter was …

Determining The Effects Of Different Bypass Fat Supplementation On Performance, Milkyield, And Milk Composition Of Anatolian Buffaloes, Sezer Öz, Seher Küçükersan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to explore the effects of animal bypass fat (ABF) and palm bypass fat (PBF) added to the rations of Anatolianbuffaloes on dry matter consumption, feed conversion ratio, milk yield, some milk components, and parameters. A total of 21 heads ofbuffalo in the early lactation period; milk yield averages, lactation periods, lactation numbers, live weights, and ages were selected anddivided into three experimental groups, each consisting of 7 buffaloes. The experiment was conducted according to the crossover trialpattern. There was no statistically significant difference between the groups in terms of dry matter consumption and feed conversionratio (p > 0.05). …

Measurement Of 8-Hydroxy-2′-Deoxyguanosine In Serum And Cerebrospinal Fluid Of Horses With Neuroaxonal Degeneration And Other Causes Of Proprioceptive Ataxia, Megan Palmisano, Jeaneen Kulp, Susan Bender, Darko Stefanovski, Mary Robinson, Amy Johnson

Faculty, Staff and Student Publications

BACKGROUND: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses.

HYPOTHESIS: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses.

ANIMALS: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November …

Measurement Of 8-Hydroxy-2′-Deoxyguanosine In Serum And Cerebrospinal Fluid Of Horses With Neuroaxonal Degeneration And Other Causes Of Proprioceptive Ataxia, Megan Palmisano, Jeaneen Kulp, Susan Bender, Darko Stefanovski, Mary Robinson, Amy Johnson

Faculty, Staff and Student Publications

BACKGROUND: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses.

HYPOTHESIS: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses.

ANIMALS: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November …

Efficacy Of Bacillus-Based Probiotic And Nspase Supplementation On Growth Performance And Gut Health Of Broilers Fed High Level Of Palm Kernel Meals Under High Stocking Density, Nurul Imawati

Chulalongkorn University Theses and Dissertations (Chula ETD)

Palm kernel meal (PKM) is a by-product of the oil palm industry with significant potential in poultry feed, although its high content of anti-nutritional factors limits its utilization. It is hypothesized that dietary supplementation with fiber-degrading enzymes and probiotics may enhance the use of PKM. This study investigated the effects of Bacillus-based probiotics (PRO) and non-starch polysaccharide enzymes (NSPase, mannanase, and xylanase) on broiler performance and gut health when fed a high level of PKM under high stocking density (HSD, Experiment 1) and normal stocking density (NSD, Experiment 2). In Experiment 1, 4,480 mixed-sex Ross308 broilers were allocated to eight …

The Development Of A Lateral Flow Immunochromatographic Strip Test For Evaluation Of Specific Antibody Levels Against Porcine Epidemic Diarrhea Virus In Pig Milk, Patumporn Jermsutjarit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea virus (PEDV) causes severe watery diarrhea in pigs of all ages, but it is particularly devastating in neonatal piglets, where it can result in 100% mortality. Therefore, lactogenic immunity is crucial for protecting against PEDV infection and reducing its virulence. One method for controlling PEDV outbreaks involves using feedback as a protocol, which entails inoculating pregnant sows with infected-PEDV intestines from piglets to enhance lactogenic immunity against PEDV. Interestingly, high serial passage of PEDV has been shown to reduce the virus's virulence. To understand the effects of serial passage, the PEDV was serially passaged both in vitro …

The Development Of A Differentiating Of Infected From Vaccinated Animals (Diva) Elisa To Detect Antibody Against Senecavirus A, Parin Watcharavongtip

Chulalongkorn University Theses and Dissertations (Chula ETD)

Senecavirus A (SVA) is the causative agent of porcine idiopathic vesicular disease (PIVD), a condition clinically indistinguishable from other vesicular diseases in pigs, such as foot and mouth disease (FMD). This similarity complicates differential diagnosis, impacting the global swine industry, including Thailand. Rapid and accurate diagnostic methods are essential for effective disease control. Therefore, developing a highly sensitive and specific diagnostic tool for detecting SVA infection is crucial for distinguishing it from other vesicular diseases and controlling its spread. In this study, we conducted a retrospective analysis of SVA emergence in diagnostic samples from 2010 to 2021 in Thailand, with …

Development Of An Endometrial Cell Culture System Of Thai Swamp Buffalo (Bubalus Bubalis) For Studying The Endometrial-Embryo Interactions, Ruchikon Jongsuwanwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Developing and optimizing endometrial sampling techniques are critical for advancing reproductive management and research in cattle and swamp buffalo. This PhD thesis aims i) to design a novel endometrial sampling device for cows and swamp buffaloes, ii) to develop a minimally invasive endometrial cell sample collection technique in cows and buffaloes to prepare endometrial cell culture, iii) to validate the developed mixed endometrial cell culture system retrieved by brush cytology techniques in cows and swamp buffaloes and iv) to utilize the minimally invasive endometrial cell sampling technique for investigation of reproductive disease in ruminant herds. The research is consequently structured …

Proteomic Analysis Of Saliva, Serum And Tissue In Feline Mammary Tumors Using Maldi-Tof Mass Spectrometry And Lc-Mass Spectrometry/Mass Spectrometry, Weejarin Paphussaro

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline mammary carcinomas (FMC) are commonly found in female cats. Early detection is necessary to prevent both local and distant metastasis and increase survival times. However, the current protein biomarkers of FMC are limited. Several biomarkers of cancers have been discovered by mass spectrometry (MS)-based proteomics, such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and liquid chromatography-mass spectrometry/ mass spectrometry (LC-MS/MS) which high sensitivity and accuracy for identified biomarker in various cancer. The purpose of this study was to identify protein expression in saliva, serum and tissue in FMC using MALDI-TOF MS and LC-MS/MS. Results of …

Molecular Characterization And Immunohistochemical Investigation Using Anti-Capsid Protein Antibody Of Canine Circovirus Associated With Respiratory Diseases In Thai Dogs, Wichan Dankaona

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine circovirus (CanineCV) is an emerging virus linked to various diseases in dogs, especially respiratory and gastrointestinal ailments. This thesis focuses on molecular characterization and histopathological investigation of CanineCV in dogs from Thailand, aiming to provide insights into the role of virus in canine respiratory diseases. The study involved genomic-based surveillance of CanineCV from nasal and oral swabs of infected dogs by employing polymerase chain reaction (PCR) assay. Additionally, the in situ hybridization (ISH) technique was used to identify and localize the viral genome within infected tissues. The CanineCV recombinant capsid gene (rCap) and protein (rCap) were synthesized and characterized, …

Impacts Of Pcv3 And Pcv2 On Reproductive Performance In The Vietnamese Industrial Sow Herds, Tien Tran Nam

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine circovirus (PCV) 2 is a globally prevalent virus causing negative impacts on the swine industry. Controlling PCV2 with vaccines is a mandatory requirement in the farming procedures depending on the prevalence of the virus and farm management system including vaccination program. It should be noted that PCV2d management in the studied farms appeared under control via the effective vaccination program. Since the first descriptions of PCV3 in the US, many publications on the viral pathogenesis and effects on susceptible animals have been conducted, but complete consensus has not been reached. Therefore, an alternative approach to studying the virus is …

Use Of Ultrasonography For Evaluation Of Response To Chemotherapy In Canine Multicentric Lymphoma, Somchin Sutthigran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine multicentric lymphoma (CML) is a common hematopoietic neoplasm in dogs that initially responds well to treatment but often relapses due to chemotherapy resistance. Evaluating the treatment response is a critical factor in helping veterinary oncologists plan and adjust chemotherapeutic protocols. This study aims to investigate the use of basic diagnostic imaging techniques, such as ultrasound (US), advanced imaging, such as computed tomography (CT) and hematologic and blood chemistry parameters, to enhance the evaluation of treatment response for this disease. This prospective observational study included 95 lymphomatous lymph nodes (LLNs) from 15 dogs with CML and 60 normal lymph nodes …

Vaccination Against Streptococcus Iniae And Bacillus Probiotic For Health Enhancement In Juvenile Barramundi (Lates Calcarifer), Pornpawit Tanpichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Juvenile Asian seabass (Lates calcarifer) with an average body weight of 10 ± 0.7 g were used to investigate the efficacy of an inactivated Streptococcus iniae vaccine and the probiotic Bacillus subtilis. Fish were intraperitoneally vaccinated with 1012 CFU fish−1 of formalin-killed S. iniae (V) or sham vaccinated (S) with sterile normal saline solution (NSS). Both groups were fed with Bacillus probiotic (5 × 109 CFU kg-1 diet) for 6 weeks. The protective efficacy of the vaccine against S. iniae examined at 4, 10 and 20 weeks post-vaccination showed that vaccinated (V) and vaccinated and probiotic (VP) groups had no …

The Impact Of Cow Behaviors During Transition Period On Reproductive And Milk Productive Performance, Aqeel Raza

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates the impact of behavioral patterns during the transition and early postpartum periods on reproductive and lactation outcomes in tropical dairy cows, using the AfiTag-II biosensor to monitor activity, rest time, rest per bout, and restlessness ratio. Data from 298 prepartum and 347 postpartum Holstein Friesian cows, categorized by lactation number (1, 2, and ≥3), was analyzed. K-means clustering on 227 lactation records identified distinct behavioral groups, facilitating the assessment of reproductive traits through Cox regression and lactation curves using the Wood model. First-lactation cows had shorter prepartum rest durations (568.8 ± 5.4 min/day) compared to higher-parity cows, …

Genetic Characteristics Of Salmonella Carrying Plasmid-Associated Antimicrobial Resistance And Virulence Genes Isolated From Pigs, Pork, And Humans In The Bordering Provinces Between Thailand And Some Land Neighboring Countries, Siraphatson Wetchasirigul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Antimicrobial resistance (AMR) is a major public health threat. The issue has disproportionately affected in low- and middle-income countries including Thailand and its neighboring counties where high rates of resistant Salmonella have been continuously reported in pigs, pork, and humans. Recognizing the significant role of R plasmids in AMR distribution, knowledge on virulence genes associated plasmids is still limited. The frequency and patterns of virulence factors are still not routinely tracked. This study aimed to characterize Salmonella enterica isolates carrying plasmid-associated virulence and AMR genes from pig, pork, and human isolates in border provinces of Thailand, Lao PDR, Cambodia, and …

Spatial Analysis Of Socioeconomic Factors At Found Locations Of Orphaned Mammals In Champaign County, Illinois, Usa, Colleen Elzinga, Stephanie Heniff, William Sander, William Marshall Brown, Csaba Varga, Samantha Sander

Human–Wildlife Interactions

Young mammals make up a significant portion of animals brought into wildlife rehabilitation centers. Unfortunately, some of these presumed orphaned individuals are inappropriately removed from their native habitat. As such, there is a need for educational community outreach centered on the proper identification of orphaned wildlife that benefit from human intervention, while maximizing limited resources of rehabilitation facilities. This study evaluated found locations of common mammalian orphans presenting from Champaign County to the University of Illinois Wildlife Medical Clinic, Illinois, USA, between 2015 and 2020. The found locations of the orphans (99 Virginia opossums [Didelphis virginiana], 80 common …

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm

Turkish Journal of Veterinary & Animal Sciences

Genetic diseases play an increasingly important role in cattle breeding as breeding technologies such as artificial insemination has an inborn risk of disseminating deleterious genes, especially from high merit breeding sires. Cholesterol deficiency (CD) is a metabolic disorder first recognized in Holstein cattle in 2015. The condition is characterized by chronic diarrhea and unthriftiness in calves being homozygous for the defective allele. The prevalence of carriers of CD in Turkish cattle is at present unknown and therefore 700 heifers and cows of different breeds, including 300 Holsteins were genotyped. Of the tested Holsteins, 2.0% turned out to be carriers of …

Validation Of A Chimeric Proteins-Based Elisa For The Diagnosis Of Brucellosis In Kazakhstan, Aitbay Bulashev, Saule Eskendirova, Bakytkali Ingirbay, Alfiya Syzdykova, Fariza Zhagipar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to compare the suitability of three chimeric recombinant outer membrane proteins of Brucella (rOmps 19 + 25, 19 + 31, and 25 + 31) for the diagnosis of bovine and sheep brucellosis using an indirect ELISA (i-ELISA). The diagnostic properties of these proteins in an i-ELISA were studied using blood serum samples from cattle and sheep with known positive and negative results for brucellosis based on the rose Bengal plate test (RBT), complement fixation test (CFT), and a commercial ELISA (c-ELISA) kit, as well as using animal sera from fresh foci of infection with unknown reactivity. rOmps19 …

Effect Of Thermo-Insulated Kid Hutch On Location Preference, Growth Performance, And Blood Haemato-Biochemical Indices Of Winter-Born Goat Kids (Capra Hircus), Ajoy Das, Dilip Kumar Mandal, Asish Debbarma, Muthupalani Karunakaran, Anupam Chatterjee, Debajyoti Sarkar

Turkish Journal of Veterinary & Animal Sciences

This study assessed the effect of thermo-insulated kid hutch (TIKH) on location preference, body surface temperature, growth performance, and blood haemato-biochemical indices of winter-born Black Bengal goat kids. Does were maintained in the same shed, born kids (n = 12) were divided into two groups along with mothers to begin experiments from day 1 of birth. The treatment group (TIKH) of kids was given a TIKH to which kids had free access, but not the adults. However, in the control group (CONT) kids were reared without TIKH. The floor surface temperature of TIKH was significantly (p < 0.05) higher at night as compared to goat shed in December (4.28%), January (3.98%), and February (7.72%). The interior roof surface of TIKH had 25.12%, 33.55%, 31.85%, 10.99%, and 4.37% higher (p < 0.01) temperatures at night compared to the roof surface temperature of the goat shed in December, January, February, March, and April, respectively. The presence of kids inside the TIKH at the age of 1–15 days was the greatest in morning (80.5%) and evening (72.2%). Between the ages of 16–-60 days, the preference of kids for the TIKH varied from 69.2% to 54.2% in the morning and from 70.0% to 66.7% in the evening. However, at the age of 61–75 days, kids’ preference for TIKH gradually declined in the morning (11.6%) and evening (19.9%) due to the increased inside temperature. Kids weaning weight was 23.25% higher (p < 0.05) in the TIKH group as compared to that of CONT. The average daily gain (0–30 days) was higher (41.34%; p < 0.05) in kids in the TIKH compared to the CONT shed. The haemato-biochemical profiles of kids were similar in both groups except for blood serum glucose, which was greater (p < 0.05) in kids of the TIKH than that of CONT shed. In conclusion, the provision of TIKH improved the growth and overall performance of winter-born Black Bengal goat kids during pre and postweaning periods.

Determining The Quality Of Sugar Beet Pulp, Lenox, And Ryegrass Silages Used In Feeding Dairy Cattle, In Comparison To Corn Silage, Fatma Özkan, Suphi̇ Deni̇z

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the quality of sugar beet pulp (Beta vulgaris L.), lenox (Brassica rapa L.), and ryegrass (Lolium multiflorium cv. caramba) silages used in feeding dairy cattle, in comparison to corn silage. Whole corn was harvested at the dough stage, lenox was harvested at the end of flowering, and ryegrass was harvested at the beginning of spike. No additives were added to the corn silage, but 5% molasses was added to the sugar beet pulp, ryegrass, and lenox silages that have low levels of easily degradable sugar. Additionally, 5% sainfoin was added to the sugar …