Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022,
2024
Faculty of Veterinary Science
Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lumpy skin disease (LSD) is a vector-borne viral disease of cattle caused by the Lumpy skin disease virus (LSDV). LSD is considered as an emerging infectious disease in cattle in Asia and poses a significant threat to cattle production across Africa, Europe, and Asia. This study aimed to describe the epidemiological characteristics of LSD outbreaks in beef cattle in Thailand and to conduct a molecular survey of LSDV infection in beef cattle from May 2021 to December 2022. The study included 594 beef cattle from 257 farms affected by the LSD outbreaks in 28 provinces. We collected hair samples (n=492) …
Isolation, Molecular Characterization, And Epidemiological Analysis Of An Emerging Avian Metapneumovirus Subgroup B In Us Poultry,
2024
South Dakota State University
Isolation, Molecular Characterization, And Epidemiological Analysis Of An Emerging Avian Metapneumovirus Subgroup B In Us Poultry, Sumit Jangra
Electronic Theses and Dissertations
Avian metapneumovirus (aMPV) is one the most economically important poultry respiratory pathogens belonging to the family Pneumoviridae and genus Metapneumovirus, which can affect both domestic and wild birds. aMPVs are classified into four subgroups (A, B, C, and D) based on the level of genetic variations and antigenic differences. aMPV causes turkey rhinotracheitis (TRT) and swollen head syndrome (SHS) in turkeys and chickens respectively. Recently, outbreaks of respiratory disease in meat-type birds and a significant decline in egg production with respiratory signs in breeders were reported from different states in the USA. Isolating aMPV is challenging as it is a …
Comparison Of Nonlinear Models For Predicting Live Weight Growth Curves In Lambproduction Of Kıvırcık And Karacabey Merino,
2024
TÜBİTAK
Comparison Of Nonlinear Models For Predicting Live Weight Growth Curves In Lambproduction Of Kıvırcık And Karacabey Merino, Vasfi̇ye Kader Esen, Orhan Karadağ, Cengi̇z Elmaci, Seli̇m Esen
Turkish Journal of Veterinary & Animal Sciences
To achieve sustainable lamb production, it is crucial to determine the optimal slaughter age for maximum economic efficiencyand have a comprehensive understanding of breed characteristics. This study focuses on evaluating different nonlinear models todetermine the optimal slaughter age for Karacabey Merino and Kıvırcık lambs, renowned for their early development and superiormeat quality, respectively. The dataset comprised age-related live weight data from 98 lambs, including 36 female and 15 male Kıvırcıklambs, as well as 33 female and 14 male Karacabey Merino lambs. Six models, namely, negative exponential, Brody, Gompertz, logistic,Bertalanffy, and Richards were utilized to analyze the data. The model parameters …
Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs,
2024
Faculty of Veterinary Science
Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs, Apichart Prapattanakullawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen responsible for substantial economic losses in the global swine industry, adversely affecting both reproductive and respiratory health. In Thailand, early PRRSV infections in suckling and nursery pigs are commonly observed, necessitating the implementation of early vaccination strategies. However, early vaccination in young piglets has been controversial due to concerns about interfering maternal-derived antibodies (MDA) and the competency of the piglets' immune system. The objective of this study is to evaluate the efficacy of a modified-live virus (MLV) PRRS vaccination in early-age piglets from PRRSV-positive farms by assessing immune responses …
Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok,
2024
Faculty of Veterinary Science
Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok, Thawanrut Kiatyingangsulee
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to 1) determine the prevalence of ESBL-producing and fluoroquinolone-resistant Salmonella in pork, 2) investigate their genomic characteristics, and 3) estimate the risk of consumer exposure. Pork samples were collected from 58 markets across 19 districts in Bangkok. The study focused on third-generation cephalosporin resistance mediated by ESBLs and fluoroquinolone resistance, representing horizontal and vertical gene transfer, respectively. Out of 793 pork samples, 339 (42.8%) were positive for Salmonella, yielding 531 isolates from 33 serotypes. The most common were Salmonella Rissen (22.6%), Sinstorf (16.8%), and Derby (10.2%). Antimicrobial susceptibility testing showed 28.2% of isolates were ESBL producers and …
Efficacy Of Cell-Free Supernatants Based Nano-Application From Thai Probiotic Bacteria As An Alternative Preservative In Meat Against Foodborne Pathogens,
2024
Faculty of Veterinary Science
Efficacy Of Cell-Free Supernatants Based Nano-Application From Thai Probiotic Bacteria As An Alternative Preservative In Meat Against Foodborne Pathogens, Thin Zar Soe
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study evaluates whether the use of cell-free supernatants (CFS) derived from Thai probiotic bacteria as natural preservatives in meat products, provides a safer alternative to chemical preservatives. The objectives include developing a nano-encapsulation technique to enhance the stability and efficacy of CFS, and assessing the effectiveness of nano-encapsulated CFS (Nano-CFS) in maintaining meat safety and quality during storage and evaluating the antibacterial efficacy of CFS and (Nano-CFS) against common foodborne pathogens, determining the minimal inhibitory and bactericidal concentrations (MIC and MBC). The CFS was prepared from the Thai probiotic strains. Antibacterial activity was determined using agar well diffusion and …
Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle,
2024
Faculty of Veterinary Science
Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle, Wannapol Buthasane
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Asian king vulture (Sarcogyps calvus, AKV), an ecologically important forest scavenger, is an Old World vulture in the family Accipitridae. It is classified as a critically endangered species, with no genomic data currently available. This dissertation aimed to assemble a high-quality reference genome to enhance understanding of its genetic background, scavenging adaptations and genetic diversity within Thailand’s captive population. A hybrid genome assembly combining Pacific Biosciences (PacBio) long-read and MGI short-read sequencing was generated from DNA extracted from the whole blood of a female individual. Comparative genomic analyses with New World vultures (NWVs) and other Accipitridae species revealed distinct …
The Comprehensive Study Of The Reproductive Cycle With A Special Focus On The Vestibular Microbiome In Captive Asian Elephants (Elephas Maximus),
2024
Faculty of Veterinary Science
The Comprehensive Study Of The Reproductive Cycle With A Special Focus On The Vestibular Microbiome In Captive Asian Elephants (Elephas Maximus), Yuqing Yang
Chulalongkorn University Theses and Dissertations (Chula ETD)
This dissertation aimed to advance the understanding of reproductive physiology and health in Asian elephants, providing critical insights and innovative methodologies for reproductive management. Chapter II established a foundational framework for normal reproductive cycling, detailing the luteal and follicular phases, and introduced a simplified, practical body condition score (BCS) method as a diagnostic tool for assessing general health and welfare. It highlighted unresolved reproductive issues, such as abnormal cycling, and revealed that progesterone peaks, with the majority (53.57%), and the onset of the follicular phase predominantly occurred during the rainy season. Monitoring ovulation and maintaining a standard BCS during the …
Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination,
2024
Faculty of Veterinary Science
Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination, Myzatul Hanis Zahiyah Yusof
Chulalongkorn University Theses and Dissertations (Chula ETD)
Arcobacter butzleri is an emerging zoonotic pathogen associated with gastrointestinal illnesses in humans, yet standardized antimicrobial susceptibility testing (AST) methods and interpretive breakpoints for this microorganism remain unavailable. Broth microdilution, despite being endorsed by the CLSI-VAST subcommittee, is often impractical in several laboratories. Therefore, this study aimed to evaluate agar dilution as a more feasible alternative to broth microdilution. A total of 415 A. butzleri isolates from various meat sources in Bangkok, Thailand were confirmed by PCR and tested against ciprofloxacin, erythromycin, gentamicin, and tetracycline. AST was conducted using broth microdilution under aerobic conditions for 24 hours as the reference …
Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats,
2024
Faculty of Veterinary Science
Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats, Run Sakulsirajit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Chronic kidney disease (CKD) is a one of the most common disorder in cats, particularly in aging populations. Early detection and accurate monitoring are crucial for effective disease management. This study aims, first, to evaluate the ultrasonographic parameters, including renal cortical thickness (RCT), medullary thickness (MT), renal length (RL), abdominal aorta diameter (Ao) and ratios, including RCT/Ao, RCT/MT, RCT/RL, MT/Ao and RL/Ao compared between clinically healthy and CKD cats. Second, to evaluate the correlations of ultrasonographic parameters with blood renal functions including blood urea nitrogen (BUN), creatinine (Cr) and symmetric dimethylarginine (SDMA) and also correlation with age, body weight (BW) …
Complete Genome Analysis Of African Swine Fever Virus Strains In Thailand,
2024
Faculty of Veterinary Science
Complete Genome Analysis Of African Swine Fever Virus Strains In Thailand, Anwesha Prakash
Chulalongkorn University Theses and Dissertations (Chula ETD)
African Swine Fever Virus (ASFV) is a large double-stranded DNA virus responsible for the highly contagious and lethal African Swine Fever (ASF), affecting the global porcine industry. The virus has a genome length of 170-194 kb and is broadly categorized into the left variable region (LVR), the right variable region (RVR), and the central conserved region (CCR). In Thailand, ASF was officially confirmed in January 2022 and identified as genotype II, which is primarily responsible for the current Eurasian swine pandemic. To date, due to the absence of a globally accepted vaccine against ASFV, culling and quarantine remain the primary …
Canine Cricoid Cartilage And Tracheal Dimensions Observed On Computed Tomographic Image,
2024
Faculty of Veterinary Science
Canine Cricoid Cartilage And Tracheal Dimensions Observed On Computed Tomographic Image, Tanya Kasemsuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Inhalation anesthesia is commonly used for general anesthesia due to its effectiveness. During the procedure, an endotracheal tube (ET) is essential for maintaining anesthetic depth, delivering anesthetic agents, and providing sufficient oxygen to reduce the risks of morbidity and mortality. Mispositioning and inappropriate ET size can lead to complications like hypoxemia, hypercapnia, and airway trauma. Commercially available ET tubes are designed for general use and are not specifically optimized for different cephalic types, leading to the objective of this study, which was to investigate the differences in anatomical dimensions of the cricoid cartilage and trachea and ET location among brachycephalic, …
Effect Of Four Non-Slipping Floor Mat Types On The Stifle Joint Range Of Motion During Walking And Trotting In Small Breed Dogs With Medial Patellar Luxation Grade 2 And Normal Stifle Dogs,
2024
Faculty of Veterinary Science
Effect Of Four Non-Slipping Floor Mat Types On The Stifle Joint Range Of Motion During Walking And Trotting In Small Breed Dogs With Medial Patellar Luxation Grade 2 And Normal Stifle Dogs, Panisara Wongsirichatchai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The two dimension kinematic motion analysis was an accessible tool for clinical approach for assist gait analysis and joint mobility in veterinary clinical field. Thermographic imaging also considered as an adjunctive non-invasive technique to detects changed superficial temperature from the physiological associated with inflammation or musculoskeletal conditions especially in joints, tendon, or muscle. A comparing controlled experiment investigated the effects of various ceramic flooring surfaces on gait, stifle joint mobility, and post-activity inflammatory response following activity in small breed dogs with and without grade 2 medial patellar luxation (MPL).Sixteen small breed dogs were enrolled, including normal stifle dogs (n=7) and …
Validation Of A Pseudotype-Based Serum Neutralization Test Forpeste Des Petits Ruminants Virus (Pprv) Specific Antibody In Goat Sera,
2024
Faculty of Veterinary Science
Validation Of A Pseudotype-Based Serum Neutralization Test Forpeste Des Petits Ruminants Virus (Pprv) Specific Antibody In Goat Sera, Suphatcha Mahawattana
Chulalongkorn University Theses and Dissertations (Chula ETD)
Peste des petits ruminants (PPR) is a transboundary viral disease in small ruminants, caused by a morbillivirus called peste des petits ruminants virus (PPRV). Recently, FAO and WOAH have set the goal to eradicate the disease in 2030. To maintain disease vigilance, the Department of Livestock Development conducts regular screenings for anti-PPRV antibodies using a commercially available competitive ELISA (cELISA). However, limitation from its window of uncertainty could lead to inconclusive result. In this study, a recently developed pseudotype-based neutralization test was validated against a large number of known positive and negative samples. In comparison with the cELISA, from 416 …
Proteomic Analysis Of Canine Reproductive Protein Profiles In Relation To Stage Of Oocyte Development,
2024
Faculty of Veterinary Science
Proteomic Analysis Of Canine Reproductive Protein Profiles In Relation To Stage Of Oocyte Development, Larindhorn Udomthanaisit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The domestic dog has a unique reproductive physiology in which oocytes are ovulated at an immature stage and complete nuclear maturation within the oviduct approximately 48–72 hours after ovulation. This thesis aimed to investigate proteomic changes in both serum and oviductal secretions associated with oocyte development, using a combination of natural and hormonally induced ovulation models. To evaluate dynamic changes in serum proteins across the estrous cycle, samples from healthy bitches in four stages—anestrus, proestrus, estrus, and diestrus—were analyzed, revealing 38 significantly altered proteins. Among these, eight exhibited stage-specific expression patterns, including HES2, ADGRA3, and SWI5 in anestrus; polycystin receptors …
Alternative Approaches To Replace Antibiotics Use In Boar Semen Extenders And Their Effects On Reproductive Performance,
2024
Faculty of Veterinary Science
Alternative Approaches To Replace Antibiotics Use In Boar Semen Extenders And Their Effects On Reproductive Performance, Congbang Ngo
Chulalongkorn University Theses and Dissertations (Chula ETD)
Artificial insemination (AI) with antibiotic-free alternatives in boar semen extenders is becoming essential for promoting environmentally sustainable practices in the global swine industry. Comprising 4 parts, this thesis aimed to investigate the boar fecal and seminal microbiomes in relation to semen quality and probiotic supplementation in an antibiotic-free feeding program. In addition, the characteristics of semen doses treated with alternative methods and their effects on the reproductive performance of sows inseminated with these semen doses are examined. (i) To investigate the fecal and seminal microbiomes of boars in relation to semen quality and the effects of transitioning from antibiotics to …
Molecular Investigation And Genetic Characterization Of Hemotropic Mycoplasma Spp. And Potential Role Of Flea Vectors In Goats In Thailand,
2024
Faculty of Veterinary Science
Molecular Investigation And Genetic Characterization Of Hemotropic Mycoplasma Spp. And Potential Role Of Flea Vectors In Goats In Thailand, Muhammad Shair Zaman Ghauri
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate the identification of flea species infesting goats and to study the molecular prevalence and genetic characterization of hemotropic mycoplasmas in goats and fleas. A total of 500 blood and 79 flea samples were collected from goat farms across seven provinces in Thailand between January 2017 and October 2023. Among the 500 goats examined, 6.6% were infested with fleas, which were morphologically identified and molecularly characterized using mitochondrial (cox-I, cox-II) and nuclear (ITS1, EF-1α) markers as Ctenocephalides orientis. Additionally, 14,315 bp fragment of the C. orientis mitochondrial genome was analyzed, revealing a close evolutionary relationship with …
Selection Of Lactic Acid Bacteria Probiotics From Thai-Chick And Their Efficacy In Restoring Gut Microbiome And Salmonella Reduction In Broiler Challenged With Salmonella Typhimurium,
2024
Faculty of Veterinary Science
Selection Of Lactic Acid Bacteria Probiotics From Thai-Chick And Their Efficacy In Restoring Gut Microbiome And Salmonella Reduction In Broiler Challenged With Salmonella Typhimurium, Benjamas Khurajog
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial resistance (AMR), food safety, and sustainable poultry farming are critical global concerns driving the exploration of probiotics as alternatives to antibiotics. This study focused on the isolation, characterization, and evaluation of lactic acid bacteria (LAB) strains and the performance of two probiotics—ProCU (a blend of locally isolated LAB strains) and TOP GUT (a commercial Clostridium butyricum product)—in mitigating Salmonella infection, improving growth performance, and modulating the gut microbiota and resistome in broiler chickens. From 91 LAB isolates identified in chicken feces across Thailand, five strains (Ligilactobacillus salivarius BF12 and Pediococcus acidilactici BF9, BF14, BYF20, and BYF26) exhibited strong tolerance …
Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs,
2024
Faculty of Veterinary Science
Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs, Panumas Kongpanna
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study evaluated the effects of dietary supplementation with a multi-strain probiotic containing Bacillus subtilis (5 × 107 CFU/g) and Lactobacillus plantarum (1 × 109 CFU/g) on reproductive performance in multiparous sows and growth performance in weaned piglets. In Experiment 1, 300 sows were assigned to control or probiotic diets across five parity groups (2, 3, 4, 5 and 6 to 9) using a 2 × 5 factorial design. Experiment 2 involved 120 weaned piglets randomly allocated to four dietary treatments: control, antibiotic, enzyme, or probiotic. Measurements included reproductive and litter performance, colostrum IgG, milk composition, and piglet growth, digestibility, …
Detection Of Brafv595e Gene Of Canine Urothelial Carcinoma Using Real-Time Pcr,
2024
Faculty of Veterinary Science
Detection Of Brafv595e Gene Of Canine Urothelial Carcinoma Using Real-Time Pcr, Monticha Kitnitchee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine urothelial carcinoma (CaUC) is the most common malignant tumor of the urinary bladder in dogs. This cancer has aggressive behavior and it is challenge for diagnosis. Most CaUC cases are diagnosed from the obvious clinical signs as the late to advance stage. In addition, therapeutic strategies for this stage are almost non-responsive for the eradication and prevention of recurrent disease. Therefore, non-invasive early diagnosis is required for urothelial carcinomas in both human and animals including dogs, which have similar cancer biology to human. This study aimed to evaluate diagnostic accuracy by BRAFV595E detection using real-time PCR (qPCR) from various …
