Veterinary Medicine Commons^™

Investigation Of Molecular Detection Rate And Associated Risk Factors Of Psittacine Beak And Feather Disease Virus In Psittaciformes In Iran, Mojtaba Khosravi, Shoreh Alian Samakkhah, Rahem Khoshbakht

Turkish Journal of Veterinary & Animal Sciences

Psittacine beak and feather disease virus (PBFDV) is a significant viral pathogen affecting the development of beak and feather cells, leading to feather abnormalities in avian species. This study aimed to investigate the molecular detection rate of PBFDV among Psittaciformes in Iran and to evaluate the influence of factors such as sex, species, season, and bird origin on PBFDV prevalence. Feather samples were collected from 1335 Psittaciformes, including Agapornis roseicollis, Pyrrhura molinae, Myiopsitta monachus, Aratinga solstitialis, Nymphicus hollandicus, Psittacula krameri, Psittacus erithacus, Poicephalus senegalus, Eos bornea, and Agapornis fischeri, and subjected to polymerase chain reaction (PCR) for PBFDV detection. The …

Experimental Sars-Cov-2 Infection Of Elk And Mule Deer, Stephanie M. Porter, Airn E. Hartwig, Helle Bielefeldt-Ohmann, Jeffrey M. Marano, J. Jeffrey Root, Angela M. Bosco-Lauth

United States Department of Agriculture Wildlife Services: Staff Publications

To assess the susceptibility of elk (Cervus canadensis) and mule deer (Odocoileus hemionus) to SARS-CoV-2, we performed experimental infections in both species. Elk did not shed infectious virus but mounted low-level serologic responses. Mule deer shed and transmitted virus and mounted pronounced serologic responses and thus could play a role in SARS-CoV-2 epidemiology.

Antimicrobial Tolerance In Salmonella: Contributions To Survival And Persistence In Processing Environments, Tomi Obe, Aaron S. Kiess, Ramakrishna Nannapaneni

Poultry Science Faculty Publications and Presentations

Salmonella remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While these chemicals have been effective, Salmonella has shown the ability to survive and persist in poultry processing environments. This can be credited to its microbial ability to adapt and develop/acquire tolerance and/or resistance to different antimicrobial agents including oxidizers, acids (organic and inorganic), phenols, and surfactants. Moreover, there are several factors in processing environments that can limit the efficacy of these antimicrobials, thus allowing survival and persistence. This mini-review examines the antimicrobial activity of common disinfectants/sanitizers used …

Effects Of Environmental Enrichments On Welfare And Hepatic Metabolic Regulation Of Broiler Chickens, Seong W. Kang, Karen D. Christensen, Michael T. Kidd, Sara K. Orlowski

Poultry Science Faculty Publications and Presentations

The aims of this study were to find suitable environmental enrichment (EE) and evaluate the combined effect of two EEs, variable light intensity (VL) lighting program and EH, on mental health and hepatic metabolic regulation in commercial broilers. To find the advantageous EEs for broilers, three different EEs (board, hut, and ramp) were tested in trial 1. EEs were placed and the engagement of birds to EEs, dustbathing behavior, and daily physical activity were observed. Birds treated with huts showed higher engagement than the board- or ramp-treated birds (p < 0.05). The results of dustbathing behavior and daily physical activity indicated that the environmental hut (EH) is the most favorable enrichment for broilers. In the second trial, to test the effect of EHs on mental health and hepatic metabolic conditions, the brain and liver were sampled from the four treatment birds (20 lx_Con, 20 lx_Hut, VL_Con and VL_Hut) on day 42. The lower expression of TPH2 (tryptophan hydroxylase 2) of VL_Hut birds than those of VL_Con and 20 lx_Hut treated birds suggests the combining effect of EHs with the VL lighting program on the central serotonergic homeostasis of broilers. Reduced expressions of TH (tyrosine hydroxylase), GR (glucocorticoid receptor), BDNF (brain-derived neurotrophic factor) of VL_Hut treated birds compared to those of VL_Con and 20 lx_Hut birds suggest lower stress, stress susceptibility, and chronic social stress in VL_Hut treated birds. The expression of CPT1A (carnitine palmitoyl transferase 1) increased over three-fold in the liver of VL_Con birds compared to 20 lx_Con birds (p < 0.05). EHs treatment in VL birds (VL_Hut) significantly decreased CPT1A but not in 20 lx birds (20 lx_Hut). The expression of ACCα (acetyl-CoA carboxylase alpha) was significantly decreased in VL_Con birds compared to 20 lx_Con birds. There was no significant difference in the hepatic FBPase (fructose-1,6-bisphosphatase), GR, and 11β-HSD1 (11 β-hydroxysteroid dehydrogenease-1) expression between 20 lx_Con and VL_Con birds, but EHs significantly stimulated GR in 20 lx_Hut birds, and stimulated FBPase and 11β-HSD1 expression in the VL_Hut birds compared to 20 lx_Con birds, suggesting that the VL lighting program reduced fatty acid synthesis and increased fatty acid β-oxidation in the broilers’ liver and VL_Hut improved the hepatic de novo glucose production. Taken together, the results suggest that the stimulated voluntary activity by EHs in the light-enriched broiler house improved mental health and hepatic metabolic function of broilers and may indicate that the improved hepatic metabolic function contributes to efficient nutritional support for broilers.

Investigation Into Cardiacmyhc-Α 334–352-Specific Tcr Transgenic Mice Reveals A Role For Cytotoxic Cd4 T Cells In The Development Of Cardiac Autoimmunity, Meghna Sur, Mahima T. Rasquinha, Kiruthiga Mone, Chandirasegaran Massilamany, Ninaad Lasrado, Channabasavaiah B. Gurumurthy, Raymond A. Sobel, Jay Reddy

Jay Reddy Publications

Myocarditis is one of the major causes of heart failure in children and young adults and can lead to dilated cardiomyopathy. Lymphocytic myocarditis could result from autoreactive CD4+ and CD8+ T cells, but defining antigen specificity in disease pathogenesis is challenging. To address this issue, we generated T cell receptor (TCR) transgenic (Tg) C57BL/6J mice specific to cardiac myosin heavy chain (Myhc)-α 334–352 and found that Myhc-α-specific TCRs were expressed in both CD4⁺ and CD8⁺ T cells. To investigate if the phenotype is more pronounced in a myocarditis-susceptible genetic background, we backcrossed with A/J mice. At the fourth …

Gene Editing Of Pigs To Control Influenza A Virus Infections, Taeyong Kwon, Bianca L. Artiaga, Chester D. Mcdowell, Kristin M. Whitworth, Kevin D. Wells, Randall S. Prather, Gustavo Delhon, Mark Cigan, Stephen N. White, Jamie Retallick, Natasha N. Gaudreault, Igor Morozov, Juergen A. Richt

School of Veterinary and Biomedical Sciences: Faculty Publications

Proteolytic activation of the hemagglutinin (HA) glycoprotein by host cellular proteases is pivotal for influenza A virus (IAV) infectivity. Highly pathogenic avian influenza viruses possess the multibasic cleavage site of the HA which is cleaved by ubiquitous proteases, such as furin; in contrast, the monobasic HA motif is recognized and activated by trypsin-like proteases, such as the transmembrane serine protease 2 (TMPRSS2). Here, we aimed to determine the effects of TMPRSS2 on the replication of pandemic H1N1 and H3N2 subtype IAVs in the natural host, the pig. The use of the CRISPR/Cas 9 system led to the establishment of homozygous …

Nuclear Phosphoproteome Reveals Prolyl Isomerase Pin1 As A Modulator Of Oncogene-Induced Senescence, Rodrigo Mohallem, Uma K. Aryal

Purdue University Libraries Open Access Publishing Fund

Mammalian cells possess intrinsic mechanisms to prevent tumorigenesis upon deleterious mutations, including oncogene-induced senescence (OIS). The molecular mechanisms underlying OIS are, however, complex and remain to be fully characterized. In this study, we analyzed the changes in the nuclear proteome and phosphoproteome of human lung fibroblast IMR90 cells during the progression of OIS induced by oncogenic RASG12V activation. We found that most of the differentially regulated phosphosites during OIS contained prolyl isomerase PIN1 target motifs, suggesting PIN1 is a key regulator of several promyelocytic leukemia nuclear body proteins, specifically regulating several proteins upon oncogenic Ras activation. We showed that PIN1 …

Naturally Derived Phenethyl Isothiocyanate Modulates Induction Of Oxidative Stress Via Its N-Acetylated Cysteine Conjugated Form In Malignant Melanoma, Sotiris Kyriakou, Nikoletta Demosthenous, Tom Amery, Kyle J. Stewart, Paul G. Winyard, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Phenethyl isothiocyanate (PEITC) is a secondary metabolic product yielded upon the hydrolysis of gluconasturtiin and it is highly accumulated in the flowers of watercress. The aim of the current study was to assess the role of a naturally derived PEITC-enriched extract in the induction of oxidative stress and to evaluate its anti-melanoma potency through the regulation of its metabolism with the concurrent production of the N-acetyl cysteine conjugated by-product. For this purpose, an in vitro melanoma model was utilized consisting of human primary (A375) cells as well as metastatic (COLO-679) malignant melanoma cells together with non-tumorigenic immortalized keratinocytes (HaCaT). …

A Potentially Cat-Astrophic Missed Diagnosis: Systemic Autoimmune Disease, Or Something More Sinister?, Cameron Smith, Do, Mph, Amy Dechet, Md

Poster Presentations

No abstract provided.

Aged 539 Internship Report: Improving Cal Poly Rodeo Horses' Health And Fitness, Faith Star Mahallati

Agricultural Education: Graduate Internship Reports

This study was conducted to help enhance the overall health and fitness of the horses on the Cal Poly Rodeo Team. Data was collected before and after implementing the StallGrazer feeders to reduce the risk of sand colic and improve feed efficiency. Data was collected and analyzed before and after the weekly barrel racing jackpot series to enhance horse fitness. The StallGrazer feeders reduced the risk of sand clear and forage waste and the barrel racing jackpots enhanced the fitness and performance of the horses in the test group.

8th Cauthen Seminar (2024) Program, Cauthen-Seminar

Veterinary Science Presentations

Contained here is the complete program for the 8th Annual Cauthen Seminar held at the Gluck Equine Research Center.

The Cauthen seminar series was started in 2017 as a tribute and memorial to respected and beloved farrier and teacher, “Tex” Cauthen, developed to encourage the sharing of best practices, technology, research, experience, and insight among all communities in the equine industry, from owners, farriers, track managers, veterinarians, and jockeys.

The annual series has been hosted at the Gluck Equine Research Center at the University of Kentucky in Lexington, KY along with a live broadcast as a Zoom webinar, offered free …

2024 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow- calf Nutrition, Reproduction, and Management

• Impact of Increasing Level of Milk Production on Cow- Calf Performance in Nebraska Sandhills
• Artificial Insemination of Beef Heifers with Multi- Sire Semen
• Effect of Heifer Percent Mature Body Weight at Breeding on Heifer Performance, Calf Production, and Subsequent Pregnancy Rates
• Effect of Glucogenic Feed Additive on Reproductive Performance in Young Postpartum Range Cows
• The Effect of Late Gestation Supplementation Strategy on Cow- Calf Performance in March- Calving Mature Cows
• The Impact of Pre- partum Supplementation Strategy on Cow- Calf Performance in May- Calving Mature Cows
• The Effect of Postpartum Supplementation Strategy on Performance …

Predicting Live Body Wight Of Yearling Beef Heifers Using 3d Imaging, Dalton J. Anderson, Yijie Xiong, Andrea K. Watson, J. Travis Mulliniks

Nebraska Beef Cattle Reports

This study was conducted to determine the accuracy of using 3D imaging technology as a method to predict shrunk body weight (BW) of growing yearling beef heifers. Red Angus x SImmental heifers (n = 69, BW = 726 ± 62 lbs; 12 months of age) were utilized for data collection. A time-of-flight depth camera (Azure Kinect, Microsoft) was used to collect depth videos as heifers walked out of the chute. Ideal image frames were identified from videos and used to determine the body volume of each heifer. Prediction of BW using images produced an R² (estimate of model fit) …

Impact Of Varying Inclusion Of Modified Distillers Grains Plus Solubles Compared To Constant Inclusion On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca Mcdermott, Mitch M. Norman, J. C. Macdonald, Rick A. Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of varying inclusion of modified distillers grains plus solubles on a weekly basis with two inclusions of grass hay on the performance of finishing steers. no interaction was observed between modified distillers grains plus solubles inclusion (0%, 25% constant, or 25% varying from 15-35%) and hay inclusion (6% or 12%). When evaluating the effect of hay inclusion on performance, cattle fed 6% grass hay had less dry matter intake than those fed 12% grass hay, and there was a tendency for gains to be greater for cattle fed 6% grass hay. Adding …

Impact Of Feeding New Fractioinated Distillers Grains (Fiber Plus Syrup) On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca L. Mcdermott, Mitch M. Norman, James C. Macdonald, Rick Stock, Ryan A. Mass, Galen E. Erickson

Nebraska Beef Cattle Reports

Fractionation processes are being explored to supply higher protein distillers grains for premium markets. Resulting distillers grains after a fraction is isolated will still be marketed as a cattle feed so knowing the impact on performance is important. This study evaluated feeding wet or dry conventional distillers grains with wet and dry fractionated distillers (fiber plus syrup) fed at 0, 20, or 40% of diet DM. When compared to the corn control, intake and gain increased for each of the four distillers types were fed, but feed conversion was poorer (i.e., increased) for dry distillers grains and dry fiber plus …

Comparison Of Corn Silage And Earlage In Finishing Diets When Fed As A Roughage On A Neutral Detergent Fiber Basis, Garrett N. Lemon, Rebecca Mcdermott, Alfredo Dicostanzo, Galen E. Erickson, Pablo L. Loza

Nebraska Beef Cattle Reports

The finishing trial was conducted to evaluate the ability of earlage to serve as a roughage source compared to corn silage when both sources were balanced on an NDF basis. Steers were fed in a randomized block design with a 2x2 treatment arrangement, with one factor being NDF source (corn silage or earlage) and the other factor being inclusion ("normal" amount of roughage provided by corn silage or earlage as the only grain source and roughage source). No significant interactions were observed between roughage source and inclusion level on steer performance. Steers fed corn silage and grain had greater intakes …

Nebraska Producer Perceptions On The Role And Implications Of Negotiations In Fed Cattle Transactions, Taralee Hudson, Elliott James Dennis, Brad D. Lubben, James C. Macdonald

Nebraska Beef Cattle Reports

A survey of ten feelot operators, as identified through the Nebraska Department of Agriculture's (NDA) 2019-2020 Cattle Feeder's Directory was conducted to identify the important concepts related to negotiated transactions and price discovery within the fed cattle market. The surveyed feedlots account for approximately 10 percent of the cattle on feed identified within the directory, on a one time capacity basis. Conducted in April of 2023 via phone conversation, the questions were related to 1) negotiated cash transactions, 2) the bidding process, and 3) thinning cash trade. Such results lend insight into the marketing practices that currently dominate the fed …

Evaluation Of Gas Emissions From Cattle On Different Diet Adaptation Strategies Using Either Forage Or Ramp, Cindy D. Mansfield, Rebecca Mcdermott, Braden C. Troyer, Mitchell M. Norman, Maggie Youngers, Andrea K. Watson, Rick Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

A 173-day finishing experiment was conducted to evaluate the effects of feeding RAMP (Cargill Corn Milling, Blair, NE) during diet adaptation compared to a traditional forage adaptation program on methane and carbon dioxide emissions, animal performance and carcass traits in beef steers. Cattle were monitored using a calorimetry emission barn to quantify production of methane and carbon dioxide during step 1 of grain adaptation and at two subsequent times while fed a common finishing diet. Feeding RAMP reduced methane by 12% during the initial diet (step 1) compared to a traditional diet that contained 43% forage. When cattle were fed …

Rethinking Corn Residue: Effects Of Grain Yield On Quality And Quantity Of Residue, Kaylee E. Wheeler, Jessica Miller, Sadie Ference, Stephan Geu, Bill Struckmeyer, Amanda Easterly, Cody F Creech, Brian Maust, Daren D. Redfearn, Jay Parsons, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A two-year experiment evaluated the effects of corn grain yield on the resulting quality and quantity of corn residue. Among the wide variety of corn hybrids, locations, and growing conditions, observed grain yields ranged from 120 to 350 bushels per acre. As corn yield increased, the total pounds of residue increased. However, the amount of reside relative to grain decreased. Within the residue, the proportion of leaf increased from 10 to 28% as corn yield increased while husk remained unchanged at 13%. Overall, the yield of leaf and husk per bushel of corn grain was not affected by grain yield …

Survey Of Current Management Practices And Evaluation Of Their Impact On Nutrient Content Of Small Cereal Silage In Nebraska, Alexa R. Johnson, Mary E. Drewnoski, Daren D. Redfearn, Susan Vanderplas, Jenny Rees, Ben Beckman, Erin Laborie, Todd Whitney, Kim Clark, Brad Schick, Gary Lesoing, Conner Biehler

Nebraska Beef Cattle Reports

The nutrient content of small cereal silage from 16 producers in Nebraska was measured at harvest and post-fermentation. At packing, 42% of the samples were below the target dry matter of 30-35%. Samples with dry matter percentage below 30% had a significant increase in the loss of energy (total digestible nutrient) content of the silage. The wetter silage appeared to have increased rates of clostridial fermentation as indicated by production of butyric acid. These data suggest that moisture management is a challenge and increased attention to ensuring the target dry matter content is achieved before packing could improve the quality …