Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand,
2024
Chulalongkorn University, College of Public Health Science
Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan
The Thai Journal of Veterinary Medicine
The presence of extended-spectrum beta-lactamase and colistin resistance E. coli in food-producing animals is a public health concern. This study was conducted using a rigorous methodology to survey the prevalence of ESBL-producing and colistin-resistant E. coli from pig farms in the central part of Thailand. A total of 519 samples were collected from fecal, feed, and waste in 53 farms located in central Thailand. All samples were inoculated onto selective agar, and species identification was performed using biochemical assays and MAlDI-TOF MS. Antimicrobial susceptibility was determined by broth microdilution, and ESBL production was confirmed by the combination disc diffusion method. …
A Preliminary Study Of Lasia Spinosa Thw. Feed Supplementation Improving Semen Quality Of Subfertile Purebred Boars,
2024
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand and Faculty of Veterinary Science, Prince of Songkla University, Songkhla, Thailand
A Preliminary Study Of Lasia Spinosa Thw. Feed Supplementation Improving Semen Quality Of Subfertile Purebred Boars, Supitcha Kaewma, Junpen Suwimonteerabutr, Nutthee Am-In, Saritvich Panyaboriban, Mongkol Techakumphu, Theerawat Swangchan-Uthai
The Thai Journal of Veterinary Medicine
Poor semen quality is a significant issue among imported purebred boars in Thailand, affecting both semen quality itself and reducing productivity at AI stations. The present study investigated the effects of dietary supplementation with Lasia spinosa Thw. (LST) on improving boar semen quality. A group of boars experiencing poor semen quality were monitored, and three were selected due to their persistent low semen profiles for the supplementation experiment. Dried LST powder was prepared in small bags at a dosage of 4 g per 100 kg body weight and added to the regular feed for daily oral administration over an 8-week …
Quantitative Evaluation Of Different Fixatives For Masson Trichrome Staining In Canine Intestinal Tissue: An Image Analysis-Based Comparative Study,
2024
Chulalongkorn University
Quantitative Evaluation Of Different Fixatives For Masson Trichrome Staining In Canine Intestinal Tissue: An Image Analysis-Based Comparative Study, Siriluck Juntausa, Kongkiat Srisuwatanasagul, Kittipot Kongsonthana, Punnarat Vibulchan, Sayamon Srisuwatanasagul
The Thai Journal of Veterinary Medicine
Neutral buffer formalin (NBF) has been widely used as a fixative for histological staining for ages. However, for some special staining, such as Masson trichrome staining, it has been proposed that the suitable fixative was Bouin’s solution. The purpose of the present study was to compare the different fixatives and post-fixative protocols on the quality of Masson trichrome staining. The canine intestines (n = 10), which were the duodenum and colon, were used as the candidate organs since they comprised of various tissue components. The fixatives and staining protocols were divided into four groups, which were Groups 1 and 2; …
Preventing And Managing Equine Gastric Ulcer Syndrome,
2024
Utah State University
Preventing And Managing Equine Gastric Ulcer Syndrome, Kylie Bell, Karl Hoopes
All Current Publications
Current industry trends regarding equine management practices largely contribute to the development of equine gastric ulcer syndrome (EGUS). Research on effective medical management exists; however, we lack studies on the efficacy of equine-welfare-minded solutions. This fact sheet reviews current literature, including diagnosing, treating, and preventing EGUS. It also provides a general overview of the natural equine, promoting a greater understanding of the connection between strategies to prevent and manage EGUS.
Intradermal Vaccination With A Phytoglycogen Nanoparticle And Sting Agonist Induces Cytotoxic T Lymphocyte-Mediated Antitumor Immunity,
2024
Purdue University
Intradermal Vaccination With A Phytoglycogen Nanoparticle And Sting Agonist Induces Cytotoxic T Lymphocyte-Mediated Antitumor Immunity, Juan F. Hernandez-Franco, Imran M. Jan, Bennett D. Elzey, Harm Hogenesch
Purdue University Libraries Open Access Publishing Fund
A critical aspect of cancer vaccine development is the formulation with effective adjuvants. This study evaluated whether combining a cationic plant-derived nanoparticle adjuvant (Nano-11) with the clinically tested STING agonist ADU-S100 (MIW815) could stimulate anticancer immunity by intradermal vaccination. Nano-11 combined with ADU-S100 (NanoST) synergistically activated antigen-presenting cells, facilitating protein antigen cross-presentation in vitro and in vivo. Intradermal vaccination using ovalbumin (OVA) as a tumor antigen and combined with Nano-11 or NanoST prevented the development of murine B16-OVA melanoma and E.G7-OVA lymphoma tumors. The antitumor immunity was abolished by CD8+ T cell depletion but not by CD4+ T cell depletion. …
Intraluminal Tracheal Stenting In Dog With Tracheal Collapse : A Case Report,
2024
Thonglor Pet Hospital Rama IX
Intraluminal Tracheal Stenting In Dog With Tracheal Collapse : A Case Report, Ratiporn Tantisak, Siriluck Luckanahasaporn, Ardong Atiptamvaree, Tamolwan Anyamaneecharoen, Kamonchanok Lamsaman, Suphat Soeratanapant, Poondej Charunmethee
The Thai Journal of Veterinary Medicine
A 12-year-old female neutered dog was referring to the pet hospital for the chief complaint of syncopic episodes 3 times/day. The dog had history of severe goose-honking non-productive cough and tachypnea. The dog got worsen despites medical management was consist of antibiotic, mucolytic, bronchodilator, glucocorticoids and nebulization. The dog was not response to medication then develop to apnea due to obstructive breathing. The radiographs diagnosis from private clinic was redundant trachea with narrowing intrathoracic trachea. The surgical treatment was considered. The tracheobronchoscopy was confirmed Tracheal collapse at mid cervical to cranial thoracic part. Stenting was a treatment options in life-threatening …
Application Of Metoclopramide As Gastroprokinetic In Koi Carp (Cyprinus Carpio) With Positive Buoyancy Disorders,
2024
Chulalongkorn University
Application Of Metoclopramide As Gastroprokinetic In Koi Carp (Cyprinus Carpio) With Positive Buoyancy Disorders, Pornpawit Tanpichai, Thanaboon Katechaimongkol, Patharapol Piamsomboon, Thanida Haetrakul
The Thai Journal of Veterinary Medicine
A two-year-old Koi carp or fancy carp (Cyprinus carpio) with a symptom of recurrent positive buoyancy was admitted to the Veterinary Medical Aquatic Animal Research Center of Excellence (VMARCE), Faculty of Veterinary Science, Chulalongkorn University. Radiographic examination with positive contrast media revealed gas accumulation in the gastrointestinal tract without obstruction; subsequently, idiopathic intestinal pseudo-obstruction was suspected. The anti-flatulence drug simethicone was administered, but the fish did not respond to the treatment. Therefore, metoclopramide, as the medical trial at a dosage of 0.5 mg/kg, was intramuscularly injected once daily for four consecutive days. The fish convalesced with neutral buoyancy …
Cardiac Biomarkers For Monitoring Canine Heartworm Disease: A Comparative Study Of N-Terminal Pro B Type Natriuretic Peptide And Cardiac Troponin I Levels,
2024
Chulalongkorn University
Cardiac Biomarkers For Monitoring Canine Heartworm Disease: A Comparative Study Of N-Terminal Pro B Type Natriuretic Peptide And Cardiac Troponin I Levels, Sariya Asawakarn, Kittipong Tachampa, Piyanan Taweethavonsawat
The Thai Journal of Veterinary Medicine
Canine heartworm disease is a global mosquito-borne parasitic illness that affects dogs. The main species of filarids responsible for this disease is Dirofilaria immitis. This article specifically explores the use of cardiac biomarkers, such as N-terminal pro B type natriuretic peptide (NT-proBNP) and cardiac troponin I (cTnI), for diagnosing and monitoring canine heartworm disease. These biomarkers can indicate myocardial injury and heart failure, particularly in chronic infections. The study compares the levels of NT-proBNP and cTnI in dogs with heartworm disease, both clinically diagnosed cases and those without symptoms. The results reveal that cTnI is elevated in both cases, …
Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs,
2024
Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University
Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Pasakorn Brikshavana, Sumit Durongphongtorn
The Thai Journal of Veterinary Medicine
Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds, leading to clinical signs and physical capacity impairment. The degree of stenotic nares was reported to be related to BAOS severity in French bulldogs. This study aimed to compare clinical signs and physical capacity in French bulldogs before and after BAOS surgery using modified Roedler questionnaires and a 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study (non-BAOS control (n=7), non-severe stenotic nares BAOS dogs (n=8), and severe stenotic nares BAOS dogs (n=12). BAOS surgery included staphylectomy using bipolar …
Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation,
2024
Faculty of Veterinary Medicine, Stara Zagora
Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation, Radka S. Garnoeva
The Thai Journal of Veterinary Medicine
The operative technique for medial patellar luxation (MPL) is dependent on the degree of accompanying bone deformities. The most frequent abnormalities: the shallow trochlear groove and tibial tuberosity medialisation are corrected surgically with trochleoplasty and tibial tuberosity transposition, respectively. The aim of the present study was to determine the distance between the tibial tuberosity and the trochlear groove (TT-TG) on two-dimensional axial computed tomography (CT) scans and three-dimensional multiplanar reconstruction images from 21 stifles from healthy dogs and 15 stifles from dogs with grade 2 MPL and to compare the performance of both methods. Two axial CT scans of the …
Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand,
2024
The International Graduated Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing
The Thai Journal of Veterinary Medicine
Gastrointestinal infectious diseases caused by bacteria have been a major problem in the swine industry, including Thailand. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis – an obligate intracellular bacterium. PPE has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied to the seroprevalence of pathogen-antibodies and the timing of L. intracellularis infection when introduced to the herd. This present study showed the current serological status and the seroprevalence of antibodies against L. intracellularis in large-scale Thai swine …
Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors,
2024
Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine
Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış
The Thai Journal of Veterinary Medicine
Mammary tumors are the most common neoplasm in dogs, with a high mortality rate. The development of canine mammary tumors (CMT) is multifactorial, but different incidence rates between breeds suggest the effects of genetic risk factors. Many CMT-associated candidate genes were reported, mainly involved in cell cycle control. This study aims to investigate p27 tumor suppressor gene polymorphisms in dogs with mammary tumors and analyze the association between variations and CMTs. For this purpose, case and control groups were formed from 22 dogs diagnosed with malignant mammary tumors and 10 dogs with healthy mammary glands. The whole canine p27 gene …
Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells,
2024
Srinakharinwirot University
Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti
The Thai Journal of Veterinary Medicine
Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …
Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023),
2024
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand
Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023), Shanaporn Leelakajornkit, Natchanon Dumniem, Permsak Wataganara, Siwa Maison, Suppawiwat Ponglowhapan
The Thai Journal of Veterinary Medicine
Surgical sterilization stands as a key solution to address the overpopulation of dogs and cats, with numerous influencing factors impacting the success of population control efforts, including species, gender, and financial conditions. The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or COVID-19, in 2020 has significantly altered human life and impacted pet animals. Lockdown restrictions were implemented in various countries, including Thailand, affecting the cumulative data of neutered animals, which was subsequently analyzed in this study. There were 337,590 dogs and cats from 2009 to 2023, and the data were systematically categorized by years, species, and gender. …
Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports,
2024
Circus animal hospital
Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports, Takuma Imai
The Thai Journal of Veterinary Medicine
The chemotherapy for canine cutaneous epitheliotropic T cell lymphoma (CETL) might cause unpleasant side effects or fail to produce positive results. We attempted a different approach with a more tolerable treatment option. We administered a high dose of oclacitinib (1.6 mg/kg/twice daily) to a patient diagnosed with CETL through histopathological examination and immunocytochemical staining. The patient had previously shown a poor response to chemotherapy and severe side effects to steroids. Within one month of starting oclacitinib treatment, we observed significant improvement in clinical symptoms, followed by improved appetite, increased activity, and weight gain after 2.5 months. However, subsequently, the patient's …
A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog,
2024
Near East University, North Cyprus
A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur
The Thai Journal of Veterinary Medicine
A young male Dachshund dog who came to the animal hospital was diagnosed with conjunctivitis on clinical examination. Methicillin-resistant Staphylococcus pseudintermedius was identified as a result of culture and bacteriological examination of the conjunctival swab, and then antimicrobial susceptibility test. Methicillin resistant S. pseudintermedius identified with the VITEK-II Compact device was also confirmed by molecular methods so that susceptible antibiotics were selected for the treatment of the patient and reported. In accordance with the antimicrobial susceptibility results, the patient was treated with an eye drop preparation containing neomycin. This is the first report of a canine conjunctivitis caused by multidrug-methicillin …
Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog,
2024
College of Veterinary Medicine, Kyungpook National University
Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog, Sang-Kwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
A 3-year-old mixed-breed dog presenting with a recurrent maxillofacial tumor was diagnosed with recurrent rhabdomyosarcoma. Computed tomography and magnetic resonance imaging revealed a left maxillofacial mass, left cervical lymph node enlargement, epidural mass dorsal to the 6th lumbar vertebra compressing the cauda equina, soft-tissue mass ventral to the 6th lumbar vertebra, multiple subcutaneous nodules and signal changes in the paravertebral muscles. The left maxilla, lumbar spine and ilium exhibited diffuse signal changes and contrast enhancement upon magnetic resonance imaging. This is, to our knowledge, the first reported case of bone marrow and epidural-space involvement of a canine rhabdomyosarcoma.
Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay,
2024
Chulalongkorn University
Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay, Kriengwich Limpavithayakul, Somsak Pakpinyo
The Thai Journal of Veterinary Medicine
Mycoplasma synoviae antibodies detection using rapid serum agglutination (RSA) assay and enzyme-linked immunosorbent assay (ELISA) is considered as the suitable screening tool in chicken flocks. Both RSA assay and ELISA have performed an ability to detect an antibody formation during mycoplasma infection due to their sensitivity in detecting both IgM and IgY. However, the IgY is the major antibody isotype transferred from the hen to the newly hatched broiler chick via egg yolk. The amount of maternal IgY circulating in the chicks’ serum is approximately 30% of the IgY level present in the hen’s bloodstream. This study was conducted to …
High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy,
2024
Sichuan Agricultural University
High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy, Ziyao Zhou
The Thai Journal of Veterinary Medicine
Laminectomy is usually used in spinal cord dysfunction like intervertebral disc herniation. High-speed pneumatic surgical drill is the most common surgical instrument for canine laminectomy operation. However, since the finical problem, the professional instrument has a low installation rate in animal hospitals in developing country. In this study, we hypothesized that dental drill may replace pneumatic surgical drills in dorsal laminectomy and hemilaminectomy, observed if dorsal laminectomy and hemilaminectomy can been successfully operated by dental drill, and compared the canine dorsal laminectomy and hemilaminectomy. Operations were performed in L2-L3 of lumbar vertebra among 12 Chinese rural dogs (6 for dorsal …
Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid,
2024
Mahasarakham University
Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich
The Thai Journal of Veterinary Medicine
The study aimed to investigate the effect of tannic acid (TA) on total antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H2O2), nitric oxide (NO), and the viability of broiler blood cells (BBC) exposed to high ambient temperatures. The BBC from a broiler chicken was exposed to temperatures ranging from 41.5°C to 46°C and incubated with TA at concentrations of 0 (the positive control group, TPCG), 5, 10, 15, and 20 μg/mL. Results showed that MDA, H2O2, and NO levels of the control group (TCG) which BBC was maintained at 41.5°C were significantly lower than TPCG. The viability of TPCG was …
