Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells,
2017
TÜBİTAK
Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells, Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk
Turkish Journal of Veterinary & Animal Sciences
Primary goat synovial cells were developed to be cocultivated with caprine arthritis encephalitis virus (CAEV)-infected monocytes. Two CAEV-seropositive goats and one CAEV-seronegative goat were confirmed for viral particles in the blood using the PCR method. Thereafter, heparinized blood samples from the three goats were collected and isolated for monocytes, which were further cultivated to differentiate into macrophages. Cultivated macrophages of 7-10 days old were cocultivated with the primary goat synovial cells for 7 days. Microscopic examination was performed every 2 days for multinucleated syncytial formation, and immunofluorescence assay was applied using monoclonal antibody against CAEV on day 7 to detect …
Effects Of Massive Ovulation On Oxidation State And Function Of The Ovaries In Laying Hens,
2017
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Effects Of Massive Ovulation On Oxidation State And Function Of The Ovaries In Laying Hens, Yu Chang, Jinghai Feng, Minhong Zhang, Liwen Jiang, Lili Zhai, Xiaolan Yang
Turkish Journal of Veterinary & Animal Sciences
Modern laying hens show fast reduction in egg production rate after 60 weeks of age, which is associated with the decline in ovarian function. The aim of this study was to examine the influence of massive ovulation on the oxidation state and ovarian function in hens. In total, 48 Jing Hong hens aged 17 weeks were randomly divided into high ovulation (HO) and low ovulation (LO) groups. The LO hens were exposed to 6 h of light per day (6 L:18 D) up to 32 weeks to delay the initiation of egg production and then were switched to 16 L:8 …
Influence Of Supplementing Diet With Microalgae (Schizochytrium Limacinum)On Growth And Metabolism In Lambs During The Summer,
2017
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Influence Of Supplementing Diet With Microalgae (Schizochytrium Limacinum)On Growth And Metabolism In Lambs During The Summer, Eki̇n Sucu, Duygu Udum, Nazmi̇ye Güneş, Önder Canbolat, İsmai̇l Fi̇lya
Turkish Journal of Veterinary & Animal Sciences
The current experiment was conducted to examine the impact of a supplement of microalgae (Schizochytrium limacinum) on the performance, rumen fermentation, and blood metabolites in lambs during summer. Forty lambs were used in a 49-day experiment. The lambs were group-fed either a basal diet (alfalfa hay and concentrate, n = 20) or the basal diet along with 5 g/day microalgae (n = 20). Feed intakes were recorded daily and body weight (BW) was measured weekly. Overall, microalgae feeding increased (P < 0.05) the BW and average daily gain. There were no significant differences for average feed intake or feed efficiency (P > 0.05). Microalgae feeding decreased rumen pH (P < 0.05) and tended to reduce proportion of acetate (P < 0.1), while it increased total rumen volatile fatty acid concentration (8.6%; P < 0.01) and proportions of propionate (13.9%, P < 0.01) and valerate (P < 0.01; 26.5%) compared with the control animals. Microalgae feeding increased (P < 0.05) blood glucose (98.47 vs. 84.97 mg/dL) and insulin (64.14 vs. 29.26 ng/mL), whereas it lowered total cholesterol concentrations in blood (62 vs. 58 mg/dL, P < 0.1) compared with the control animals. The results of this study indicate that microalgae supplement influences productivity and enhances dietary energy utilization in lambs during the summer.
Evaluation Of Diagnostic Methods For The Detection Of Pestiviruses In Clinical Samples,
2017
TÜBİTAK
Evaluation Of Diagnostic Methods For The Detection Of Pestiviruses In Clinical Samples, Peli̇n Tuncer Göktuna, Kadi̇r Yeşi̇lbağ
Turkish Journal of Veterinary & Animal Sciences
There are several commonly used diagnostic methods to detect pestiviruses for routine diagnostic purposes. In the present study, we aimed to compare virus isolation-indirect immunoperoxidase monolayer assay (VI-IIPMA), antigen capture ELISA (ACE), and RT-PCR for the detection of pestiviruses in clinical samples. Out of 246 samples tested (11 serum, 119 swab, 116 tissue), 28 samples (11.39%) were positive and 218 (88.61%) were negative using the VI-IIPMA method. Using ACE, 70 samples (28.46%) were positive and 176 (71.54%) were negative. Finally, using RT-PCR analysis, we detected 19 (7.72%) positive and 227 (92.28%) negative samples. Inconsistencies were observed among results of the …
Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni
Turkish Journal of Veterinary & Animal Sciences
Bovine parainfluenza virus type 3 (BPIV3) is a leading respiratory pathogen of cattle and is often implicated as a causative pathogen of bovine respiratory disease complex (BRDC). Three genotypes (BPIV3a-c) have been tentatively classified based on genetic and phylogenetic analyses. A BPIV3c isolate, designated NX49, was isolated using the Madin-Darby bovine kidney cell culture from cattle in western China, where feedlot cattle with severe respiratory disease symptoms were observed. Hemagglutination assay demonstrated that NX49 had low agglutination activity against guinea pig erythrocytes, whereas SD0835, the first BPIV3c strain reported in China, showed high agglutination activity. Phylogenic analysis indicated that NX49 …
Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples,
2017
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Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples, Ni̇lgün Ünal, Şi̇nasi̇ Aşkar, Murat Yildirim
Turkish Journal of Veterinary & Animal Sciences
The present study was performed to isolate and identify Enterococcus spp. from broiler cloacal samples to species level, to determine their resistance patterns to various antibiotics, and to detect vancomycin resistance genes. Cloacal samples of broilers collected from slaughterhouses were inoculated in Slanetz and Bartley agars with and without vancomycin (6 μg/mL). Antibiotic resistance/susceptibility testing of the isolated and identified enterococci was performed by using the disk diffusion test. Multiplex PCR was used to identify the species and to detect vancomycin resistance genes. The majority of the isolated enterococci was Enterococcus faecium (60.43%, n = 142) and Enterococcus faecalis (33.62%, …
Typification Of A Fresh Goat Cheese Of Mexico By Path Models,
2017
TÜBİTAK
Typification Of A Fresh Goat Cheese Of Mexico By Path Models, Emmanuel J. Ramírez-Rivera, José M. Juárez-Barrientos, Jesús Rodríguez-Miranda, Pablo Díaz-Rivera, Lorena G. Ramón-Canul, José A. Herrera-Corredor, María I. Hernández-Serrano, Erasmo Herman Y Lara
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to typify goat cheese and determine the relationship among climatic factors, production systems, milk quality, and their impact on the physicochemical, instrumental, and sensory properties and consumer preference by path models. Using the regression partial least square technique coupled to multiple factor analysis, two models were established. These models showed that the production system directly affected the physicochemical quality and indirectly affected instrumental and sensory parameters, which explained the preference and classification of the cheeses. The proposed methodology is expected to generate useful information enabling the establishment of potential fresh goat cheese produced in …
Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) Species In Thewestern Black Sea Region Of Turkey, New Records For The Turkish Fauna, Bi̇lal Di̇k, Özge Kuçlu, Rahi̇le Öztürk
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to detect biting midges of the Culicoides (Diptera: Nematocera: Ceratopogonidae) fauna in the Western Black Sea Region of Turkey between June and August 2014. In this study, 34,047 Culicoides samples were captured and 35 Culicoides species were identified. C. obsoletus (Meigen), 1818 was found to be the most dominant species. It was followed by C. picturatus Kremer & Deduit, 1961; C. punctatus (Meigen), 1804; and C. pulicaris (Linnaeus). All of the Culicoides species detected in the study were reported for the first time in the Western Black Sea Region. C. alazanicus Dzhafarov, 1961 and C. …
Some Reproductive, Lactation, And Kid Growth Characteristics Ofkilis Goats Under Semiintensive Conditions,
2017
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Some Reproductive, Lactation, And Kid Growth Characteristics Ofkilis Goats Under Semiintensive Conditions, Mahmut Keski̇n, Sabri̇ Gül, Osman Bi̇çer, İrfan Daşkiran
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine some reproductive and kid growth characteristics as well as some lactation traits of Kilis goats raised under semiintensive conditions in Kilis Province in Turkey. The animal material of the study comprised 502, 698, and 753 heads of Kilis goats in 2013, 2014, and 2015, respectively. At the end of study, multiple birth rates were calculated between 31.5% and 35.7% during the years. Lactation length and lactation milk yield were determined to be between 207.1 ± 0.81 and 232.4 ± 0.65 days and between 315.7 ± 4.16 l and 375.6 ± 3.4 L, respectively …
Modelling Beef Consumption In Turkey: The Ardl/Bounds Test Approach,
2017
TÜBİTAK
Modelling Beef Consumption In Turkey: The Ardl/Bounds Test Approach, Hamza Erdoğdu, Hasan Çi̇çek
Turkish Journal of Veterinary & Animal Sciences
The study aimed to examine the short-run and long-run relationship between beef consumption and beef prices, chicken meat prices, and per capita income for the period of 1994?2014 in Turkey by employing the ARDL/bounds test approach. After deciding on the presence of cointegration between the related variables, a parsimonious VECM model was estimated to conduct the structural analyses of the impulse response function and variance decomposition. The results of the bounds test suggest a long-run equilibrium relationship between beef consumption and its selected determinants. In addition, the empirical findings indicate that chicken meat prices and per capita income level have …
Electroencephalographic Electrode Montage In Goats: Topographical, Radiological, Andphysiological Assessment,
2017
TÜBİTAK
Electroencephalographic Electrode Montage In Goats: Topographical, Radiological, Andphysiological Assessment, Arun Kumar Madan, Sunil Kumar Rastogi, Arup Kumar Das, Jayant Pandurang Korde, Ishwar Singh, Girijesh Kumar Singh
Turkish Journal of Veterinary & Animal Sciences
The paucity of information on assortment of EEG leads in goats has hampered analysis of brain regional activity during different physiological, pathological, and ethological conditions in this species. Hence, the present study was designed to develop a montage with suitable combinations of EEG electrode arrays in goats. Ten specimens of freshly slaughtered goat heads (crossbreed of Jamunapari) were procured and preserved at -20 °C. Frozen samples were sliced sagittally and transversely to corroborate the link between intended topographic landmarks for EEG leads over the scalp and underlying provinces of the brain. Based on these observations, nine sites for EEG electrode …
The Treatment Of Supracondylar And Diaphyseal Femoral Fractures Incats Using Intramedullary Two-Way Stacked Kirschner Wire Application, Kemal Altunatmaz, Murat Karabağli, Di̇dar Aydin, Özlem Güzel, Ebru Eravci Yalin, Ümi̇t Uğurlu, Defne Joan Şadalak, Hi̇mmet Eki̇ci̇
Turkish Journal of Veterinary & Animal Sciences
Femoral fractures in cats are treated using pin and wires, lag screws, Rush pins, intramedullary threaded pins, interlocking pins, clamp rod internal fixators, and plates or plate-rods. In this study, 14 cats brought to the surgery clinic between the years 2010 and 2015 for fracture, due to either a road traffic accident or falling from a height, were evaluated. Following clinical and radiological examination of the cases, the type and location of the fracture, details of the applied fixation method, and postoperative clinical and radiological results were assessed. Localization of the fractures was as follows: 4 comminuted diaphyseal (1 segmental), …
Detection And Economic Impact Related To Bovine Respiratory Disease, Shrink, Andtraveling Distance In Feedlot Cattle In Northwest Mexico, Jose Luis Rodríguez-Castillo, Gilberto Lopez Valencia, Francisco Javier Monge Navarro, Gerado Enrique Medina Basulto, Sawako Hori-Oshima, Sergio Arturo Cueto-González, Alfonso De La Mora-Valle, Luis Mario Muñoz-Del Real, Luis Tinoco-Gracia, Tomas Benjamin Rentería-Evangelista
Turkish Journal of Veterinary & Animal Sciences
Bovine respiratory disease (BRD) is the main reason for economic loss in feedlots. A cross-sectional study was conducted in order to detect the pathogens involved in BRD. A total of 88 animals with respiratory signs were sampled for diagnosis using PCR. The detected pathogens were bovine respiratory syncytial virus (80.6%), Mannheimia haemolytica (79.5%), Pasteurella multocida (68.1%), parainfluenza 3 virus (23.8%), bovine herpes virus-1 (20.4%), and bovine viral diarrhea virus (11.3%). The average number of treatments applied per animal was 1.3 with an estimated treatment cost of 16 USD per treatment/animal. Animals receiving more than one treatment averaged costs exceeding 38 …
An Investigation Of Antibacterial Effects Of Steroids,
2017
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An Investigation Of Antibacterial Effects Of Steroids, Abdullah Doğan, Sali̇h Otlu, Özgür Çelebi̇, Pinar Aksu, Ali̇ye Gülmez Sağlam, Ali̇ Nazmi̇ Can Doğan, Nesli̇han Mutlu
Turkish Journal of Veterinary & Animal Sciences
In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterol on some bacteria were investigated. In the research, standard strains of the gram-positive bacteria Bacillus subtilis (lab isolate) and Staphylococcus aureus (ATCC 6538) and the gram-negative bacteria Escherichia coli (ATCC 25922), Pasteurella multocida (ATCC 12945), and Pseudomonas aeruginosa (ATCC 9027) were used. A modified broth dilution method was applied for determining the minimal inhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions and as a medium. At the end of the study, the MIC of corticosterone …
Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts,
2017
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Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts, Babu Lal Jangir, Raj Bahadur Bind, Pawan Kumar, Ramesh Somvanshi
Turkish Journal of Veterinary & Animal Sciences
Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus (BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphology and association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which revealed that growths were either single or multiple, variable in shape and size, and present at different body sites. Histopathologically, these were categorized as papilloma, fibropapilloma, and fibroma. Polymerase chain reaction (PCR) revealed the presence of …
Diagnosis And Treatment Of Umbilical Cord-Derived Tetanus In Neonatal Calves,
2017
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Diagnosis And Treatment Of Umbilical Cord-Derived Tetanus In Neonatal Calves, Nuri̇ Altuğ, Nazmi̇ Yüksek, Abdullah Karasu, Fatma İlhan, Ebubeki̇r Ceylan, İsmai̇l Hakki Eki̇n, Sezai̇ Arslan
Turkish Journal of Veterinary & Animal Sciences
In this study, umbilical cord-derived neonatal tetanus in calves was identified in Turkey. Four calves with tetanus-specific history and clinical findings were used. Blood samples were taken before and after treatment, and clinical findings were recorded. A tetanus-specific treatment procedure was performed. However, the calves died from not responding to treatment. Pretreatment white blood cell, peripheral blood polymorphonuclear leukocyte, creatine kinase (CK), and lactate dehydrogenase (LDH) levels were determined to be high. Aspartate aminotransferase, alanine aminotransferase, CK, and LDH activities were found to increase after treatment compared to before treatment. Gram-positive terminal spore-forming bacilli were observed in bacterioscopic examination of …
Addition Of Molasses, Corn Steep Liquor, And Rice Polish As Economical Sources To Enhance The Fungal Biomass Production Of Wheat Straw By Arachniotus Sp., Faisal Shahzad, Muhammad Abdullah, Abdul Shakoor Chaudhry, Abu Saeed Hashmi, Jalees Ahmed Bhatti, Makhdum Abdul Jabbar, Hafiz Muhammad Ali, Tauseef Rehman, Farah Ali, Mian Muhammad Khubaib Sattar, Fayyaz Ahmed, Irfan Irshad
Turkish Journal of Veterinary & Animal Sciences
This study was planned to see the additive effects of various industrial residues including molasses, rice polish, and corn steep liquor for the bioprocessing of wheat straw (WS) through solid-state fermentation (SSF) using Arachniotus sp. as a fermenting agent. The performance of fungal-based WS (seed culture) was evaluated in terms of the favorable changes in crude protein (CP) contents. WS (5 g) was mixed with our selected carbon and nitrogen sources in a basal medium containing optimized salt concentrations to attain the desired level of water and nutrients. An increased (P < 0.0001) CP value was observed at a 25:1 C:N ratio and maximum fungal protein contents (13.40%) were found by mixing 0.4 mL of molasses, 0.8 g of rice polish, and 3.0 mL of corn steep liquor in treated WS at a setting time of 48 h in SSF. It was seen that the growth rate of Arachniotus sp. was greatly improved by the addition of our selected amounts of carbohydrate and nitrogen sources. Hence, our current results support the use of these economical byproducts for cheap biomass production.
Control Of Fermented Sausage, Salami, Sausage, And Hamburger Meatballs Produced In Meat Production Facilities Applying The Iso Food Security System For Food Pathogens, Nuray Gamze Yörük, Ahmet Güner
Turkish Journal of Veterinary & Animal Sciences
In this study, fermented sausage, salami, sausage, and hamburger meatballs produced by 6 different companies were assessed for pathogen contamination. These firms were either International Organization for Standardization (ISO) 22000-compliant or non-ISO 22000 compliant. Samples were taken 4 times over 4 seasons. The total mesophilic aerobic quantities of Bacillus cereus, Clostridium perfringens, and Staphylococcus aureus, along with the presence of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7, were tested in each sample. In 96 samples, Salmonella spp. (3.12%), L. monocytogenes (17.7%), and E. coli O157:H7 (4.16%) were detected. From the ISO-certified firms, Salmonella spp. were detected in one meatball …
The Effects Of Heparin Concentration, Storage Time, And Temperature On The Values Of Hematological Parameters In Cyprinus Carpio, Malgorzata Witeska, Justyna Biardzka, Joanna Kniaz
Turkish Journal of Veterinary & Animal Sciences
The results of hematological analyses may be affected by preanalytical factors such as sampling procedure, type and concentration of anticoagulant, or conditions and time of blood storage. In the present study the effects of various concentrations of heparin (0-200 IU/cm3) and different times (2, 24, or 48 h) and temperatures (4 or 22 °C) of blood storage on the values of hematological parameters in Cyprinus carpio were evaluated. Increase in heparin concentration resulted in a decrease in frequency of erythroblasts, a reduction of leukocyte and thrombocyte counts, and a decrease in oxidative metabolic activity of phagocytes. Storage for 2 h …
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens,
2017
TÜBİTAK
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens, Sirin Theerawatanasirikul, Nunyarat Koomkrong, Autchara Kayan, Chaiwat Boonkaewwan
Turkish Journal of Veterinary & Animal Sciences
The intestinal barrier includes physical and chemical components for preventing the invasion of pathogenic and toxic agents. The aim of this study was to investigate small intestinal morphology, antimicrobial peptides, and tight junction (TJ) distribution among broilers, Thai Betong chickens, and native Thai Praduhangdum chickens. Intestinal samples from 40 chickens of each breed were collected. The results of the histological and morphological examination revealed that the duodenum of all breeds had the maximum villus height compared to the other parts. The intestinal tract of both Betong and Praduhangdum chickens was low in the number of mucin and goblet cells. In …
