Thromboelastographic Evaluation Of Hemostatic Functionin Dogs With Dilated Cardiomyopathy,
2017
TÜBİTAK
Thromboelastographic Evaluation Of Hemostatic Functionin Dogs With Dilated Cardiomyopathy, Zeki̇ Yilmaz, Meri̇ç Kocatürk, Oya Eralp İnan, Pinar Levent
Turkish Journal of Veterinary & Animal Sciences
This study aimed to evaluate the hemostatic function by thromboelastography (TEG) and determine if there was an association between the changes in hemostatic parameters and serum cardiac troponin I (cTnI) in dogs with dilated cardiomyopathy (DCM). DCM was diagnosed by echocardiography in 19 dogs. Ten healthy dogs were selected as controls. Myocardial injury was confirmed by increased serum cTnI levels. Coagulation was assessed by TEG evaluating clot kinetics (reaction time [R] and kinetic time [K]), clot strengthening (alpha [α] angle and G value), platelet function (maximum amplitude [MA] and coagulation-index [CI]), clot stability (LY30, the percentage of lysis 30 min …
Metabolic Indicators For Early Pregnancy In Zebu And Crossbreddairy Cows Reared In A Subtropical Climate,
2017
TÜBİTAK
Metabolic Indicators For Early Pregnancy In Zebu And Crossbreddairy Cows Reared In A Subtropical Climate, Muzamil Abdulah, Tushar Kumar Mohanty, Mukesh Bhakat, Arumugam Kumaresan, Tapas Kumar Patbandha, Ambadas Rakhamaji Madkar, Ashok Kumar Mohanty
Turkish Journal of Veterinary & Animal Sciences
To identify metabolic indicators in blood and urine for early pregnancy in dairy cows (32 crossbred and 12 purebred zebu cows), blood and urine samples were collected on days 0, 15, 20, 24, and 30 postbreeding. The plasma progesterone (P4) concentration was significantly (P < 0.05) higher in pregnant cows than in nonpregnant cows after day 15. The plasma blood urea nitrogen (BUN) concentration remained significantly (P < 0.05) higher in pregnant cows on day 15. The plasma concentrations of cholesterol, glucose, protein, and nonesterified fatty acid (NEFA) did not differ between pregnant and nonpregnant cows. The urine protein concentration was significantly higher (P < 0.05) in pregnant zebu cows compared to nonpregnant zebu cows. Plasma P4 was significantly (P < 0.001) and positively associated with urine glucose levels in nonpregnant crossbred cows. In nonpregnant zebu cows, the correlation between P4 was positive (P < 0.05) with plasma cholesterol, glucose, urine glucose, and protein. In pregnant zebu cows, P4 was negatively associated with plasma BUN and positively (P < 0.05) associated with both plasma and urine glucose levels. Thus, urinary proteins can be used as a noninvasive tool for early nonpregnancy confirmation in cows with very high accuracy at around 24 days after artificial insemination.
Evidence Of Foot-And-Mouth Disease Virus Excretion In The Milk Ofapparently Healthy Vaccinated Buffaloes In Islamabad, Pakistan, Hafiziftikhar Ahmed, Umer Farooq, Aamer Bin Zahur, Khalid Naeem, Asma Latif, Hamid Irshad
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples (n = 200) were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD (78%; 156/200) in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% (19/156) of the …
Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya
Turkish Journal of Veterinary & Animal Sciences
The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …
Slaughter And Carcass Characteristics Of Herik Male Lambs Raised Under A Finishing System,
2017
TÜBİTAK
Slaughter And Carcass Characteristics Of Herik Male Lambs Raised Under A Finishing System, Mustafa Uğurlu, Bülent Teke, Fi̇li̇z Akdağ, Mustafa Salman, Bülent Eki̇z, İsmai̇l Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the slaughter and carcass characteristics of Herik lambs finished under intensive husbandry conditions. Twenty single male lambs, ten of which had long, semifat tails and ten of which had short, round, and fat tails, all with an average live weight of 20 kg, were used in the study. Concentrate feed and fresh water were given ad libitum and alfalfa hay as forage was given in the amount of 300 g/lamb per day. The lambs were slaughtered when they reached a final weight of 42 kg. The average hot and cold dressing percentages, …
Effective Use Of Probiotic-Glyconutrient Combination As An Adjuvant To Antibiotic Therapy For Diarrhea In Rearing Dairy Calves,
2017
TÜBİTAK
Effective Use Of Probiotic-Glyconutrient Combination As An Adjuvant To Antibiotic Therapy For Diarrhea In Rearing Dairy Calves, Gilberto Lopez Valencia, Octavio Zapata-Ramirez, Lilia Núñez-González, Victor Núñez-Benítez, Heriberto Landeros- López, Maria Lopez-Soto, Alberto Barreras Serrano, Victor Gonzalez-Vizcarra, Alfredo Estrada-Angulo, Richard Zinn, Alejandro Plascencia
Turkish Journal of Veterinary & Animal Sciences
Thirty Holstein calves presented with infectious diarrhea during the second week following birth were utilized to evaluate the effects of a standardized product containing a combination of probiotics, ß-glucans, and glyconutrients (GLY) in support of antibiotic therapy during the early rearing phase (day 7 to day 45 following birth). Treatments were 1) antibiotic therapy (trimethoprim and sulfas; ANTIB), and 2) ANTIB plus 5 g/calf per day GLY. Treatments did not affect diarrhea duration, averaging 16.7 days. However, GLY support decreased (P < 0.05) the mortality rate and increased (P < 0.05) live weight gain. Increased weight gain was attributable to enhanced (P < 0.05) consumption of calf starter. Supplemental GLY increased (P < 0.05) serum glucose and lowered (P < 0.05) serum urea nitrogen, nonesterified fatty acids, and cortisol concentration. We conclude that daily provision of 5 g of a standardized product containing probiotics, ß-glucans, and glyconutrients to diarrheic calves under antibiotic therapy will promote health and enhance early growth performance.
Detoxification Of Mitochondrial Oxidants And
Apoptotic Signaling Are Facilitated By
Thioredoxin-2 And Peroxiredoxin-3 During
Hyperoxic Injury,
2017
Children's Health Research Center, Sanford Research, Sioux Falls, South Dakota
Detoxification Of Mitochondrial Oxidants And Apoptotic Signaling Are Facilitated By Thioredoxin-2 And Peroxiredoxin-3 During Hyperoxic Injury, Benjamin J. Forred, Darwin R. Daugaard, Brianna K. Titus, Ryan R. Wood, Miranda J. Floen, Michelle L. Booze, Peter F. Vitiello
School of Veterinary and Biomedical Sciences: Faculty Publications
Mitochondria play a fundamental role in the regulation of cell death during accumulation of oxidants. High concentrations of atmospheric oxygen (hyperoxia), used clinically to treat tissue hypoxia in premature newborns, is known to elicit oxidative stress and mitochondrial injury to pulmonary epithelial cells. A consequence of oxidative stress in mitochondria is the accumulation of peroxides which are detoxified by the dedicated mitochondrial thioredoxin system. This system is comprised of the oxidoreductase activities of peroxiredoxin-3 (Prx3), thioredoxin-2 (Trx2), and thioredoxin reductase-2 (TrxR2). The goal of this study was to understand the role of the mitochondrial thioredoxin system and mitochondrial injuries during …
Maternal Inflammation At Mid-Gestation In Pregnant Rats Impairs Fetal Muscle Growth And Development At Term,
2017
University of Nebraska-Lincoln
Maternal Inflammation At Mid-Gestation In Pregnant Rats Impairs Fetal Muscle Growth And Development At Term, C. N. Cadaret, K. A. Beede, E. M. Merrick, T. L. Barnes, J. D. Loy, D. T. Yates
School of Veterinary and Biomedical Sciences: Faculty Publications
Intrauterine growth restriction (IUGR) is a leading cause of perinatal morbidity and mortality. Low birth weight resulting from preterm birth and/or IUGR is an underlying factor in 60–80% of perinatal death worldwide, and is particularly common in developing countries (UNICEF, 2008). Furthermore, studies have linked IUGR and the associated fetal malnutrition to increased incidence of metabolic syndrome in adult life (Barker et al., 1993; Godfrey and Barker, 2000). The “thrifty phenotype hypothesis” developed by David Barker (Hales et al., 1991) states that IUGR-associated fetal malnutrition forces the fetus to spare nutrients by altering tissue-specific metabolism in order to survive. In …
A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling,
2017
University of Illinois at Urbana-Champaign
A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling, Ponnuraj Nagendraprabhu, Sushil Khatiwada, Sabal Chaulagain, Gustavo A. Delhon, Daniel L. Rock
School of Veterinary and Biomedical Sciences: Faculty Publications
Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤30 min post infection). ORFV119 NF-κB inhibitory activity was found unimpaired upon translation inhibition, suggesting that virion ORFV119 alone is responsible for early interference in signaling. A C-terminal LxCxE motif in ORFV119 enabled the protein to interact with the retinoblastoma protein (pRb) a multifunctional protein best known for its tumor suppressor activity. Notably, experiments using a recombinant virus containing an ORFV119 …
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle,
2017
TÜBİTAK
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …
The Comparison Of The Effects Of Intraarticular Injections Of Bovine Amnioticfluid And Hyaluronic Acid On Cartilage Tissue In An Experimental Osteoarthriticrabbit Model: Histopathological And Immunohistochemical Results, Murat Tanrisever, Hati̇ce Eröksüz, Sai̇t Bulut
Turkish Journal of Veterinary & Animal Sciences
In this experimental study, effects of bovine amniotic fluid and hyaluronic acid were compared on an osteoarthritic model induced in rabbit stifle joints. The study was performed on 21 rabbits undergoing anterior cruciate ligament transaction for establishing osteoarthritis. Four weeks later, the rabbits were divided into 3 equal groups of 7 animals each. The first group of rabbits received intraarticular injection of 0.5 mL of bovine amniotic fluid (BAF) three times at 1-week intervals. The second group of rabbits received intraarticular injection of 0.5 mL of hyaluronic acid (HA) three times at 1-week intervals. The third group of rabbits served …
Development And Evaluation Of Time-Temperature Integrator Formonitoring High Temperature Thawing Of Frozen Buffalo Meat,
2017
TÜBİTAK
Development And Evaluation Of Time-Temperature Integrator Formonitoring High Temperature Thawing Of Frozen Buffalo Meat, Vishnuraj Mangalathu Rajan, Kandeepan Gurunathan, Vivek Shukla
Turkish Journal of Veterinary & Animal Sciences
Research was undertaken to develop an enzyme-substrate-based time-temperature integrator (TTI) for monitoring higher temperature thawing of frozen buffalo meat. Alpha-amylase and iodine-starch complex were standardized for enzyme-substrate combinations and process optimization and packed in 2 × 2 cm LDPE bags. The meat samples were packed in polystyrene trays with cling film overwrap and frozen at -18 ± 1 °C. Each sample had a sticker-type TTI attached to the surface of the package. The frozen samples were subjected to temperature abuse at 25 ± 1 °C and 37 ± 1 °C, keeping a control at 4 ± 1 °C. On higher …
The Effect Of Whole Body Vibration On Equine Hoof Growth,
2017
Otterbein University
The Effect Of Whole Body Vibration On Equine Hoof Growth, Sharlee Lowe
Undergraduate Honors Thesis Projects
Equine foot pain can be a result of many conditions and disease processes, all of which lead to a loss of function in the horse. When the horse loads the foot, pressure applied to the frog translates to the digital cushion and lateral cartilages, compressing the blood vessels, perfusing the area, and circulating blood back up the leg; this constant flow of blood stimulates growth of the hoof wall. Whole body vibration plates are thought to result in rapid muscle contractions and subsequently, increased circulation through the foot. An increase in hoof growth could prove whole body vibration beneficial as …
The Effects Of Chemical Reagents And Physical Environment On The In Vitro And In Vivo Properties Of Adipose-Derived Multipotent Stromal Cells Isolated From Different Species,
2017
Louisiana State University and Agricultural and Mechanical College
The Effects Of Chemical Reagents And Physical Environment On The In Vitro And In Vivo Properties Of Adipose-Derived Multipotent Stromal Cells Isolated From Different Species, Wei Duan
LSU Doctoral Dissertations
Adult adipose derived mesenchymal stromal cells (ASCs) have been characterized in various species. Many factors may affect ASC fate and ASCs from different species may have different response to these factors. The first study was to identify the differences of the canine ASCs isolated from subcutaneous and infrapatellar adipose tissues, and evaluate the impact of cryopreservation on the cells. Based on paired comparisons of fresh and cryopreserved ASCs, cryopreserved ASCs had lower CD29 and CD44 protein expression and lower proliferation rates. The cryopreserved ASCs had relative lower mitochondria in the cytoplasm compared to the fresh ASCs regardless of tissue sources. …
Association Of Porcine Cryptorchidism With Dna Parentage Test, Testicular Protein Expression And Testicular Proteomics,
2017
Faculty of Veterinary Science
Association Of Porcine Cryptorchidism With Dna Parentage Test, Testicular Protein Expression And Testicular Proteomics, Nathamon Yimpring
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cryptorchidism is one of the most common congenital abnormalities observed in boars. One of the important obstacles to produce good quality pork is cryptorchidism. Pigs with cryptorchidism cause defects in spermatogenesis, low conception rate and/or infertility. This odor can be the cause of economic loss because consumers reject to buy meat with boar taint and flavor. In fattening farm, multi-sire mating system is normally used. Molecular genetic techniques allow the possibility of testing on genetic and parentage control. This research aimed to study i. To optimize microsatellite markers and develop the DNA fingerprint technique used to identify the parentage of …
Role Of Skeletal Paracrine Signals In The Proliferation And Chondrogenic Differentiation Of Interzone Cells,
2017
University of Kentucky
Role Of Skeletal Paracrine Signals In The Proliferation And Chondrogenic Differentiation Of Interzone Cells, Parvathy Thampi
Theses and Dissertations--Veterinary Science
Articular cartilage in mammals has a limited intrinsic capacity to repair structural injuries and defects, a fact that contributes to the chronic and progressive nature of osteoarthritis. Current treatment modalities do not enable articular cartilage to achieve a complete and permanent restoration of normal structure and function with large or partial thickness lesions. In contrast to mammals, Mexican axolotl salamanders (Ambystoma mexicanum) have demonstrated the remarkable ability to spontaneously and completely repair even large joint cartilage lesions, an intrinsic healing process that involves interzone cells in the intraarticular space. Further, when interzone tissue is transplanted into critical sized …
Biology And Detection Of Pregnanes During Late Gestation In The Mare,
2017
University of Kentucky
Biology And Detection Of Pregnanes During Late Gestation In The Mare, Michelle Arelia Ann Wynn
Theses and Dissertations--Veterinary Science
Progesterone in the mare declines to almost undetectable concentrations in late gestation. It’s metabolized into several pregnanes, some circulating at very high concentrations. Although the function of many pregnanes remains unclear, 5α-dihydroprogesterone and allopregnanolone are bioactive. Measurements of pregnanes in late gestation are typically by immunoassay, although results are confounded by cross-reactivity with related pregnanes. Conversely, liquid chromatography tandem mass spectrometry (LC-MS/MS) allows differentiation of individual pregnanes. The purposes of these studies were: 1) to evaluate the ability of a 5α-reductase inhibitor, dutasteride, to alter pregnane metabolism and pregnancy outcome, 2) to evaluate changes in target pregnanes in late gestation …
An Investigation Into Specific Seminal Plasma Proteins And Their Effect On The Innate Immune Response To Breeding In The Mare,
2017
University of Kentucky
An Investigation Into Specific Seminal Plasma Proteins And Their Effect On The Innate Immune Response To Breeding In The Mare, Carleigh Elizabeth Fedorka
Theses and Dissertations--Veterinary Science
The mare experiences a transient innate immune response to breeding, the resolution of which is crucial for optimal fertility. The majority of mares are able to modulate this inflammation in a timely fashion, but a subpopulation exists which fail to do so and are considered susceptible to persistent breeding-induced endometritis (PBIE). Seminal plasma has been shown to modulate aspects of this inflammation. Recently, two seminal plasma proteins have garnered interest for their immune modulating properties: cysteine-rich secretory protein-3 (CRISP-3) and lactoferrin. These proteins have been found to alter the binding between sperm and neutrophils based on sperm viability in vitro …
Analysis Of Humoral Immune Responses In Horses With Equine Protozoal Myeloencephalitis,
2017
University of Kentucky
Analysis Of Humoral Immune Responses In Horses With Equine Protozoal Myeloencephalitis, Catherine-Jane Angwin
Theses and Dissertations--Veterinary Science
Equine protozoal myeloencephalitis (EPM), caused by the protozoan parasite Sarcocystis neurona, is one of the most important neurological diseases of horses in the Americas. While seroprevalence of S. neurona in horses is high, clinical manifestation of EPM occurs in less than 1% of infected horses. Factors governing the occurrence and severity of EPM are largely unknown, although horse immunity might play an important role in clinical outcome. We hypothesize that EPM occurs due to an aberrant immune response, which will be discernable in the equine IgG subisotypes a, b, and (T) that recognize S. neurona in infected diseased horses versus …
Inflammation And Insulin Dysregulation In The Horse,
2017
University of Kentucky
Inflammation And Insulin Dysregulation In The Horse, Sarah E. Elzinga
Theses and Dissertations--Veterinary Science
Insulin dysregulation (ID) in the horse is receiving increasing attention as a serious health concern, in particular due to its association with the potentially career or life ending condition, laminitis. The role of inflammation and the immune system in ID as well as its associated health concerns has also been raised. However, the involvement of inflammation in and mechanisms behind ID in the horse remain unclear. Therefore, our overall hypothesis was that due in part to changes in their gut microbiota and plasma lipidome, horses with ID have changes in circulating proinflammatory markers, in particular in response to glycemic challenge, …
