Natural Mycoplasma Bovis Infection Associated With Epithelioid Granulomatous Caseonecrotic Bronchiolitis At A Progressed Disease Stage In Calves,
2022
Chulalongkorn University
Natural Mycoplasma Bovis Infection Associated With Epithelioid Granulomatous Caseonecrotic Bronchiolitis At A Progressed Disease Stage In Calves, Mathurot Suwanruengsri, Ryoko Uemura, Uda Zahli Izzati, Takuya Kanda, Naoyuki Fuke, Masahiro Yasuda, Takuya Hirai, Ryoji Yamaguchi
The Thai Journal of Veterinary Medicine
Mycoplasma bovis (M. bovis) is a pathogen that especially effects the respiratory system of cattle. We focused on the histopathological changes in the lungs caused by M. bovis because the lesions in the bronchi or bronchioles and alveoli are quite different in natural infections. Thirty-five lung samples from 2-to 12-month-old Japanese Black calves with respiratory symptoms were collected and examined for bacteria presence and pathological changes using immunohistochemistry (IHC). Using the loop-mediated isothermal amplification method, 18 of the 35 samples were found to be positive for M. bovis, which was isolated and identified. In 7 of the samples, only M. …
Comparison Of An Intra-Articular Suture Technique And A Lateral Suture Technique In Toy Breed Dogs With Cranial Cruciate Ligament Rupture,
2022
Chulalongkorn University
Comparison Of An Intra-Articular Suture Technique And A Lateral Suture Technique In Toy Breed Dogs With Cranial Cruciate Ligament Rupture, Sirun Tuek-Um, Chaiyakorn Thitiyanaporn, Phitchaya Phongwirat, Naris Thengchaisri
The Thai Journal of Veterinary Medicine
Cranial cruciate ligament rupture (CCLR) can result in inflammation, pain, lameness and osteoarthritis. Because there is no isometric point for surgical stabilization for CCLR, the anatomical replacement of the ruptured cranial cruciate ligament with synthetic material may provide better outcomes. This retrospective study compared short-term post-operative outcomes between an intra-articular suture technique and a lateral suture technique for treating CCLR in toy breed dogs. Twenty dogs with unilateral CCLR were divided into two groups. The lateral suture technique was a lateral circumfabellar tibial suture with monofilament nylon and the intra-articular suture was performed by passing a suture through the femoral …
Naturally Occurring Peripheral T-Cell Lymphoma Not Otherwise Specified (Ptcl-Nos) With Systemic Dissemination In A Beagle,
2022
Chulalongkorn University
Naturally Occurring Peripheral T-Cell Lymphoma Not Otherwise Specified (Ptcl-Nos) With Systemic Dissemination In A Beagle, Ploen Nganthavee, Phuttipan Channgam, Tanit Kasantikul, Nlin Arya, Sirintra Sirivisoot, Tuddow Thaiwong, Zachary Millman, Rungrote Osathanon
The Thai Journal of Veterinary Medicine
A 6-year-old intact female Beagle dog presented with acute vomiting. Abdominal ultrasound showed abdominal lymphadenopathy, ascites and a hyperechoic mass within the pancreas. Thoracic radiographs and echocardiography revealed cardiomegaly with pericardial effusion. The dog died following rapid deterioration and a subsequent necropsy revealed a multilobulated white-to-tan mass within the pancreas, attaching to the omentum, stomach and left adrenal gland. The heart was enlarged and infiltrated by similar coalescing off-white irregular masses. Histologically, the masses consisted of infiltrative sheets of large T lymphocytes replacing the normal architecture of the heart along with the lymph nodes, pancreas, stomach, omentum, left adrenal gland, …
Investigation Of Bacterial Pathogens In Milk From Mastitic Dairy Cattle By Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry,
2022
Chulalongkorn University
Investigation Of Bacterial Pathogens In Milk From Mastitic Dairy Cattle By Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry, Gokben Ozbey, Baris Otlu, Yusuf Yakupogullari, Betul Celik, Elif Seren Tanriverdi, Neslihan Kelestemur, Tarik Safak, Ali Risvanli, Anil Persad, Emma Sproston
The Thai Journal of Veterinary Medicine
The scope of the present study was to assess the use of Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry (MS) as a quick technique for the identification of bacterial species in mastitis. In this study, milk samples from each udder quarter from a total of 250 dairy cattle were aseptically collected and tested. The samples were grouped into California Mastitis Test (CMT) positive, CMT negative and clinical mastitis. The samples were streaked on blood agar and the bacterial isolates were analysed using MALDI-TOF MS. Using MALDI-TOF MS, certain species such as Staphylococcus chromogenes (44/188, 23.4%), Aerococcus viridans (40/188, 21.3%) …
Inflammatory Mediator Gene Expression Of The Kidneys Induced By Daboia Siamensis Venom And Its Components,
2022
Chulalongkorn University
Inflammatory Mediator Gene Expression Of The Kidneys Induced By Daboia Siamensis Venom And Its Components, Suchitra Khunsap, Sunutcha Suntrarachun, Orawan Khow, Jureeporn Noiphrom
The Thai Journal of Veterinary Medicine
Phospholipase A2 (PLA2) and metalloprotease (MP) from Daboia siamensis (Ds) venom are believed to contribute to renal tissue toxicity. Imbalance of the cytokine system induced by snake envenomation causes tissue injury and cell damage which leads to acute toxic kidney injury. A better understanding of the pathological mechanism involved in renal injury after venom administration is needed. In the present study, we aimed to investigate inflammatory mediator genes (IL-2, IL-10, TNFα, IFN, and COX-2) in mouse kidney tissue after venom injection. Quantitative real time PCR was performed to detect the cytokine gene profiles. Beta actin was used as a house-keeping …
Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr,
2022
Chulalongkorn University
Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul
The Thai Journal of Veterinary Medicine
The objectives in this study was to investigate the prevalence, seasonal variation and antimicrobial resistance of Salmonella isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR. During May 2018 to April 2019, 445 swab samples were randomly collected from pork (n = 258), beef (n = 81) and poultry meat (n = 106) in three seasons at three retail big markets in Vientiane capital, Lao PDR, Thongkhankham, Lao-Aussi, and Nongchan markets. All samples were isolated and identified for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 procedure. The isolates were tested for antimicrobial susceptibility using …
Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China,
2022
Chulalongkorn University
Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China, Jing Yang, Ming Li, Tianfei Yu
The Thai Journal of Veterinary Medicine
Muscovy duck parvovirus (MDPV) is fatal for young ducklings of less than 3 weeks of age and has caused severe economic loss in duck production in many countries and regions. We report here on a complete genome sequence of a recombinant MDPV strain YL08 isolated from an ill Muscovy duck in 2008 at Qiqihar, Heilongjiang province, China. This genome, MDPV YL08, was 5061 nucleotides long and retained the two ORFs, which are typical in the Parvovirinae family. The genome of YL08 strain has a putative recombination breakpoint on its VP1 gene. This study has helped our understanding of the genetic …
The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines,
2022
Chulalongkorn University
The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert
The Thai Journal of Veterinary Medicine
The PRRS virus and PED alpha-coronavirus are the two main pig pathogens in the world. The current strategies to control these diseases are inadequate. The recombinant chimeric swine PKR-Apaf-1 protein (rcPAP) consists of the Human Immunodeficiency Virus Trans-activator transcription (HIV-TAT) domain, the dsRNA-binding domain of porcine Protein kinase R (PKR) gene and the caspase recruitment domain (CARD) domain of porcine Apoptotic Protease-Activating Factor-1 (Apaf-1) gene. In general, the rcPAP can detect viral dsRNA in virally infected cells and induces apoptosis and kills the virus-infected cells. In this study, the reducing viral-load efficacy of rcPAP (produced from bacteria) against PRRS virrus …
Trace Elements, Oxidative Stress Biomarkers And Hemato-Biochemical Indices Of Goat Kids Affected With Nutritional Dependent Locomotor Disturbances,
2022
Chulalongkorn University
Trace Elements, Oxidative Stress Biomarkers And Hemato-Biochemical Indices Of Goat Kids Affected With Nutritional Dependent Locomotor Disturbances, Issam Eljalii, Taha Fouda, Wael El-Deeb, Abdulaziz Almujalli, Sabry M. El-Bahr
The Thai Journal of Veterinary Medicine
This study aimed to investigate the biomedical significant of trace elements, oxidative stress and hemato-biochemical indices in the diagnosis of locomotor disturbances of affected goat kids. Blood samples were collected from 22 goat kids affected with locomotor disturbances. An equal number of healthy goat kids was used as a control. Clinical examination showed anemia and an incoordination in affected animals compared to the control. Malondialdehyde (MDA), creatine kinase (CK) and aspartate aminotransferase (AST) values were significantly higher in affected animals than in the control, while superoxide dismutase (SOD) and reduced glutathione (GSH) values were significantly lower. Concentrations of copper (Cu), …
The Relationship Of Abortion, Dog Management And Neospora Caninum In Dairy Herds In Taiwan,
2022
Chulalongkorn University
The Relationship Of Abortion, Dog Management And Neospora Caninum In Dairy Herds In Taiwan, Hsu-Hsun Lee, Chen-Chih Chen, Yi-Lun Tsai, Shyh-Shyan Liu, Po-An Yueh, Huan-Yu Hsu, Ai-Mei Chang
The Thai Journal of Veterinary Medicine
Neospora caninum (N. caninum) has caused an abortion storm in dairy herds for two decades in Taiwan. There is no effective medicine for N. caninum infection for livestock, therefore, prevention by farm management is very important. The risk factors for N. caninum infection in Taiwan is still unclear. Dogs are the common definitive hosts of N. caninum on farms. This study aims to estimate the sero-prevalence and detection of N. caninum DNA in 361 pregnant dairy cows and establish an effective strategy to prevent abortion caused by N. caninum in dairy farms in Taiwan by logistic regression. The sero-prevalence of …
Effects Of Atipamezole And Flumazenil On Stress-Related Hormonal And Metabolic Responses In Cats Anesthetized With Medetomidine, Midazolam, Ketamine And Isoflurane Undergoing Ovariohysterectomy And Castration,
2022
Chulalongkorn University
Effects Of Atipamezole And Flumazenil On Stress-Related Hormonal And Metabolic Responses In Cats Anesthetized With Medetomidine, Midazolam, Ketamine And Isoflurane Undergoing Ovariohysterectomy And Castration, Hirokazu Kamohara, Toshiko Kamohara, Yoshiaki Hikasa
The Thai Journal of Veterinary Medicine
This study examined the post-operative effects of atipamezole (Ati) and flumazenil (Flu) on stress-related neurohormonal and metabolic responses in isoflurane-anesthetized cats premedicated with medetomidine, midazolam and ketamine and undergoing ovariohysterectomy or castration. Client-owned mixed-breed cats (n = 108) were treated with 50-μg/kg medetomidine and 0.5-mg/kg midazolam, followed by 5-mg/kg ketamine (MMK) intramuscularly (IM) and maintained under isoflurane anesthesia. The cats were divided into nine groups (six cats/group): control (saline IM), 50-μg/kg Flu IM or intravenously (IV), 100-μg/kg Ati IM or IV, 200-μg/kg Ati IM, 100-μg/kg Ati + 50-μg/kg Flu IM or IV and 200-μg/kg Ati + 50-μg/kg Flu IM. Five …
Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand,
2022
Chulalongkorn University
Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn
The Thai Journal of Veterinary Medicine
This retrospective study investigated the clinical characteristics of multidrug-resistant (MDR) Escherichia coli and its antimicrobial resistance phenotypes and analyzed the treatment outcomes of MDR E. coli infection in dogs and cats. The medical data of dogs and cats diagnosed in 2020 with E. coli infection at a veterinary teaching hospital were analyzed. Of 94 cases, the frequency of MDR E. coli (66%) infection was higher than that of non-MDR E. coli (34%). MDR E. coli was significantly more frequently detected in female dogs than non-MDR E. coli (P < 0.026). The most frequent MDR E. coli isolation sites were the urinary tract in dogs and skin wounds in cats. MDR E. coli isolates from dogs were highly resistant to ampicillin (96.1%), enrofloxacin (80.4%) and tetracycline (78.4%). Resistance to ampicillin (100%), enrofloxacin (90.9%), marbofloxacin (72.7%) and tetracycline (72.7%) occurred frequently in MDR E. coli isolates from cats. Low resistance to amikacin was detected in the MDR E. coli isolates from dogs and cats. The rates of clinical cure and non-clinical cure of the MDR E. coli and non-MDR E. coli cases were not significantly different. The duration of antimicrobial treatment for MDR E. coli was significantly longer in cats (12.6 ± 5.85 days) than in non-MDR E. coli cases (7 ± 0 days) (P < 0.048). Adjunctive therapy was prescribed more frequently in MDR E. coli (20%) than non-MDR E. coli cases (8.3%). The presence of MDR E. coli was high in this study. High resistance to commonly used antimicrobial drugs and treatment complications was observed in this study.
African Swine Fever Vaccine Development: Current Status And Challenges Ahead,
2022
Chulalongkorn University
African Swine Fever Vaccine Development: Current Status And Challenges Ahead, Sezim Monoldorova, Galina Koltsova, Ilya Titov, Inkyu Yoo, Andrey Gogin, Jonguk Jeong, Hyeon-Seop Byeon, Seungri Yun, Yunhee Chang, Shin-Seok Kang, Denis Kolbasov, Bo-Young Jeon
The Thai Journal of Veterinary Medicine
African swine fever (ASF) is a contagious, hemorrhagic and highly lethal viral disease in swine. Following the initial report of ASF in Kenya in 1921 it spread to East Asia through Western Europe and Russia (including Eastern Europe). Many efforts have been dedicated to controlling ASF but the struggle to eradicate this disease continues. Thus far, the efforts to develop an effective vaccine to control ASF have been unsuccessful. A previous inactivated ASF vaccine, developed by traditional methods, failed to protect pigs from ASF virus (ASFV) infection. Neutralizing antibodies were not effective in inducing protective immunity and it appears that …
Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey),
2022
Chulalongkorn University
Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey), Dogan Akca, Fatih Buyuk, Elif Celik, Aliye Gulmez Saglam, Salih Otlu, Serpil Dag, Ozgur Celebi, Mustafa Reha Coskun, Eray Buyuk, Emin Karakurt, Mitat Sahin
The Thai Journal of Veterinary Medicine
This study aimed to investigate Listeria species in various samples taken from aborted sheep in Kars province and to determine the position of emerged strains in the phylogenetic tree. Milk (n= 229) and vaginal swab (n= 263) samples from aborted sheep and abomasum contents (n= 46) of aborted sheep fetuses were investigated by the culture and Polymerase Chain Reaction (PCR) methods. For the phylogenetic analysis of isolates, the 16S rRNA gene region was sequenced. In the culture, the Listeria agent was not isolated from any of the vaginal swabs or milk samples. Among the 46 fetal abomasum contents, a Listeria …
Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species,
2022
Chulalongkorn University
Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan
The Thai Journal of Veterinary Medicine
The emergence of multidrug-resistant (MDR) Salmonella spp. has increased the need for investigation of antimicrobial alternatives. Ethanolic extract of propolis (EEP) has been identified to have wide-ranging medical applications related to its antimicrobial activity. The current study aimed to identify the major chemical components of EEP with antibacterial effects, evaluate the antibacterial efficacy of EEP against MDR Salmonella spp. isolates, and further examine the therapeutic efficacy of EEP against MDR S. Enteritidis infected mouse model. Bulgarian propolis EEP was prepared by using ethanol based extraction method. The chemical composition of EEP was characterized using the gas chromatography-mass spectroscopy (GC-MS). The …
Indications For Canine Fecal Microbiota Transplantation,
2022
Chulalongkorn University
Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou
The Thai Journal of Veterinary Medicine
Fecal microbiota transplantation (FMT) is a novel method to treat intestinal and extraintestinal diseases by transplanting the microbiota in the feces of healthy animals into the gastrointestinal tracts of patients and reconstructing intestinal flora in the patients' intestine. In this article, we reviewed the recent studies of canine FMT focusing on its indications. FMT was proved effective to treat canine Clostridioides difficile infection (CDI), inflammatory bowel disease (IBD), canine parvovirus (CPV) diarrhea and acute diarrhea; upregulate the intestinal microbiota in dogs with acute hemorrhagic diarrhea syndrome (AHDS); and may have benefit for preventing canine post-weaning diarrhea. However, the specific FMT …
Shock Status And Anti-Shock Infusion Therapy In Canine Acute Spontaneous Babesiosis,
2022
Chulalongkorn University
Shock Status And Anti-Shock Infusion Therapy In Canine Acute Spontaneous Babesiosis, Oksana Dubova, Lyudmila Kovalova, Anatolyi Dubovyi, Pavel Kovalov, Inna Chala
The Thai Journal of Veterinary Medicine
Canine babesiosis is a serious disease that is accompanied by the development of shock. The aim of the work was to establish shock, assess its degree, as well as to conduct a clinical trial of anti-shock infusion therapy. 100 dogs of different breeds, weighing 20–40 kg, aged 2–4 years, were involved. The studies used oscillometry (blood pressure, pulse rate), assessment of vascular-platelet hemostasis (platelet count, spontaneous aggregation ability), coagulogram (prothromine and activated partial thromboplastin time, fibrinogen), enzyme immunoassay (fibrin degradation products and D-dimer), biochemical (soluble fibrin-monomer complexes) and hemodynamic (circulating blood volume, hematocrit) methods of investigation. In dogs with acute …
Low Colostrum Immunoglobulin G Concentration In Saanen Dams With Seropositive To Caprine Arthritis Encephalitis Virus Infection Without Affecting Kid Growth Rate,
2022
Chulalongkorn University
Low Colostrum Immunoglobulin G Concentration In Saanen Dams With Seropositive To Caprine Arthritis Encephalitis Virus Infection Without Affecting Kid Growth Rate, Chollada Buranakarl, Sumpun Thammacharoen, Kazuo Katoh
The Thai Journal of Veterinary Medicine
Caprine arthritis encephalitis (CAE) is one of the most pervasive diseases and causes economic damages both to health and milk production. Data on colostral quality and kids’ growth rate of CAE-seropositive CAE dams is limited. The aims of the present study were, 1) to investigate the general components including insulin-like growth factor 1 (IGF-1), immunoglobulin G (IgG) and Vitamin A (Vit A) in colostrum and 2) to determine the effect of the separation of kids born from CAE-seropositive mothers and fed heat-processed colostrum and milk on the growth rate of the kids of Saanen goats. The colostrum of dams with …
Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia,
2022
Chulalongkorn University
Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia, Afshin Behzadian, Ameneh Khoshvaghti
The Thai Journal of Veterinary Medicine
Feline panleukopenia is a serious contagious viral disease of cats that has been identified as the causative agent of death in 25% of kittens sent for pathological examination. Antioxidant systems are one of the very important defense systems of body. The aim of the current study was to evaluate changes in serum levels of Vitamin D, glutathione peroxidase and superoxide dismutase in cats infected with panleukopenia virus. Ten healthy male cats and ten male cats with clinical and laboratory symptoms of panleukopenia with a mean age of 6±2 months were selected. The Glutathione peroxidase and superoxide dismutase were measured by …
Surveillance Of Zoonotic Brugia Pahangi In Monastery Cats, Samphran District, Nakhon Pathom, Thailand,
2022
Chulalongkorn University
Surveillance Of Zoonotic Brugia Pahangi In Monastery Cats, Samphran District, Nakhon Pathom, Thailand, Thanakorn Rawangchue, Nuttasiri Sripirom, Sivapong Sungpradit
The Thai Journal of Veterinary Medicine
The mosquito-borne filarial nematode parasites Brugia pahangi and B. malayi have been proposed as potential zoonotic pathogens, with dogs and cats being the main reservoir hosts. The monasteries where both stray and semi-domesticated animals live often serve as areas where humans are exposed to these reservoirs. In Thailand, the prevalence of B. pahangi, particularly in infected monastery cats, is underreported. In this study, we investigated B. pahangi prevalence in cats at 24 temples in the Samphran district, Nakhon Pathom province, central Thailand. In total, 196 blood samples were collected from the cats and examined using the hematocrit capillary tube technique. …
