Veterinary Medicine Commons^™

Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to objectively assess the severity of the clinical signs and physical capacity in French Bulldogs with BAOS using questionnaire and 6-minute walk test (6-MWT), respectively. A total of 26 French Bulldogs were enrolled in this study and divided into a normal group (n = 7), a moderate BAOS group (n = 8) and a severe BAOS group (n = 11). After history taking and physical examination, the questionnaire …

The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren

The Thai Journal of Veterinary Medicine

The present study determined the possible protective effect of pumpkin seed oil (PSO) on histopathological changes in liver tissue and serum alanine aminotransferase (ALT) activity, glucose, Non-Esterified Fatty Acids (NEFA) and triglyceride (TG) levels on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. A total of 80 Wistar rats were divided into eight groups of ten rats each: the first group served as the control, group II was given intraperitoneal 0.2 mL/kg CCl4, twice a week for 8 weeks. Groups III-V were treated daily with PSO for 8 weeks (1, 2 and 3 mL/kg respectively). Groups (VI-VIII) were …

Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li

The Thai Journal of Veterinary Medicine

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and …

Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul

The Thai Journal of Veterinary Medicine

The dog erythrocyte antigen (DEA) as a blood group antigen in dogs, especially DEA 1.1, has the highest immunogenicity. IgYis an excellent antibody for use in immunological assays involving mammalian sera. The aim of this study was to produce and purify IgY against DEA1.1 from chicken egg yolks for canine blood group detection. Hens were immunized by intramuscular vaccination. IgY was achieved after three booster immunizations by DEA 1.1-positive canine ghost cells. The extraction of total IgY from the egg yolk was carried out using precipitation by polyethylene glycol 6000 and dialysis. The purified protein and yield of IgY were …

Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew

The Thai Journal of Veterinary Medicine

The in vivo bone regenerative capacity and biocompatibility of bisphenol A glycidylmethacrylate (BisGMA) implants fabricated by Stereolithography Rapid Prototyping machine (SLA) wasinvestigated in rabbit’s calvarial defect model. The study focused on two different materials: BisGMA alone and hydroxyapatite (HA) incorporated BisGMA, of which were formulated especially for SLA fabrication. Each rabbit’s skull implant was fabricated with size 1x1 cm2according to the rabbit skull’s contour based on its Computed Tomographic dataand replaced at the full thickness cavarial defects in 6 rabbits. Computed Tomography (CT) scan and blood tests were performed at certain time points during 6 months after surgical operation to …

Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury

The Thai Journal of Veterinary Medicine

Infections with equine herpesviruses(EHVs) are widespread in equine populations worldwide. Whilst EHV-1 produces well-documented reproductive losses in equids, the role of asinineherpesviruses-5 (AHV-5) to induce abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no, KF434370) and Austrian …

Preparation Of Swine Isg15 Polyclonal Antibody And Its Application In Classical Swine Fever Virus, Xin-Bin Cai, Hua-Lin Hou, Li-Yuan Zhang, Rong-Ze Tang, Shan Yin, Xiao-Quan Li, Ke Zhang, Qian-Nan Zhao, Xue Ying, Li-Juan Su, Shi-Kai Sun, Xiao-Ning Li, Ting Rong Luo

The Thai Journal of Veterinary Medicine

Interferon-stimulated gene 15 (ISG15) is known to participate in innate and adaptive immune responses against viral infections. Classical swine fever virus (CSFV) causes a global, economically important and highly contagious disease in pigs. To explore theinteraction between ISG15 and CSFV, the ISG15gene was amplified, cloned and expressed in E. coliBL21 cells. The expressed ISG15 protein was purified by Ni-NTA agarose column and used to prepare a polyclonal mouse antibody. The anti-ISG15 antibody produced in mice could react with ISG15 expressed in the prokaryotic BL-21 cells as well as natural swine ISG15 expressed in PK-15 cells. This polyclonal antibody against swine …

Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso

The Thai Journal of Veterinary Medicine

Cardiac myxoma is a rare disease in dogs. Its histogenic origin has not yet been established, but the involvement of mesenchymal stem cells has been suggested in humans. Here we report an encapsulated and benign cardiac myxoma located at the entrance to the pulmonary trunk of the heart of an 8-year-old male Yorkshire Terrier dog presenting sudden death in the absence of clinical symptoms related to cardiac alterations. Hematoxylin/eosin staining demonstrated the mixoid origin of the myxoma, showing abundant hypocellular stroma with polygonal cells, while immunohistochemistry (IHC) corroborated its mesenchymal lineage with the detection of vimentin and α-SMA positive cell …

Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum

The Thai Journal of Veterinary Medicine

The present study focused on the synthesis, In Ovoantiviral evaluation and In silicostudy of most active antiviral compounds of the azo series. The synthesis of title compounds was done by the coupling reaction of diazonium salt solutions withactive methylene (1,3-dioxolane and benzimidazole), to yield [(E)-1-(1,3-dioxolan-2-yl)-2-phenyldiazene] (A1), [(E)-1-(1,3-dioxolan-2-yl)-2-(4-methyl-phenyl)diazene] (A2), 2-[(E)-phenyldiazenyl]-1H-benzimidazole] (A3) , [(E)-1-(1,3-dioxolan-2-yl)-2-(4-ethylphenyl)diazene] (A4) and [(E)-1-(1,3-dioxolan-2-yl)-2-(2-methylphenyl)diazene] (A5). The structures of newly synthesized molecules were elucidated by spectroscopic techniques (EI-MS and FT-IR). In Ovoscreening of compounds against avian influenza virus (AIV) H9N2 strain and Newcastle Disease virus (NDV) Lasota strain was done. The evaluation data suggested that azo compound (A5) exhibited the …

Vampire Bats: Preparing For Range Expansion Into The U.S., Michael J. Bodenchuk, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

The common vampire bat apparently is expanding its range northwards in Mexico and seems poised to enter the southern United States. Climate models predict suitable habitat in the U.S. in south Texas and parts of southern Arizona. While vampire bats’ northward range expansion is not unexpected, the fact that this species brings a strain of rabies that impacts livestock and people warrants a strategic response. Annual economic damages from bats are estimated between $7M and $9M, largely associated with deaths of livestock from rabies. To prepare for the emerging rabies issue, USDA Wildlife Services programs in Texas and Arizona have …

The P-Glycoprotein Repertoire Of The Equine Parasitic Nematode Parascaris Univalens, Alexander P. Gerhard, Jürgen Krücken, Emanuel Heitlinger, I. Jana I. Janssen, Marta Basiaga, Sławomir Kornaś, Céline Beier, Martin K. Nielsen, Richard E. Davis, Jianbin Wang, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

P-glycoproteins (Pgp) have been proposed as contributors to the widespread macrocyclic lactone (ML) resistance in several nematode species including a major pathogen of foals, Parascaris univalens. Using new and available RNA-seq data, ten different genomic loci encoding Pgps were identified and characterized by transcriptome-guided RT-PCRs and Sanger sequencing. Phylogenetic analysis revealed an ascarid-specific Pgp lineage, Pgp-18, as well as two paralogues of Pgp-11 and Pgp-16. Comparative gene expression analyses in P. univalens and Caenorhabditis elegans show that the intestine is the major site of expression but individual gene expression patterns were not conserved between the two nematodes. In P. …

New World Mammarenaviral Hemorrhagic Fever Virus Determinants Of Infection And Disease, Brady T. Hickerson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Several New World mammarenaviruses (NWM) are able to cause life-threatening hemorrhagic disease in humans. These agents use human transferrin receptor 1 (hTfR1), the main protein involved in cellular uptake of iron, to infect cells. Furthermore, use of hTfR1 correlates with the ability of these viruses to cause severe disease in humans. The most prevalent pathogenic NWM is Jun√≠n virus (JUNV), which is the etiological agent of Argentine hemorrhagic fever (AHF).The pathogenesis of AHF is poorly understood. A cardinal feature of severe cases of AHF is a prolonged increase of serum interferon-a (IFN-a) concentration. This increase is hypothesized to play a …

An Evaluation Of Bird And Bat Mortality At Wind Turbines In The Northeastern United States, Daniel Y. Choi, Thomas W. Wittig, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Wind energy offers substantial environmental benefits, but wind facilities can negatively impact wildlife, including birds and bats. Researchers and managers have made major efforts to chronicle bird and bat mortality associated with wind facilities, but few studies have examined the patterns and underlying mechanisms of spatial patterns of fatalities at wind facilities. Understanding the horizontal fall distance between a carcass and the nearest turbine pole is important in designing effective search protocols and estimating total mortality. We explored patterns in taxonomic composition and fall distance of bird and bat carcasses at wind facilities in the Northeastern United States using publicly …

Estimation Of Wildlife Damage From Federal Crop Insurance Data, Sophie Mckee, Stephanie A. Shwiff, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wildlife damage to crops is a persistent and costly problem for many farmers in the USA. Most existing estimates of crop damage have relied on direct assessment methods such as field studies conducted by trained biologists or surveys distributed to farmers. In this paper, we describe a new method of estimating wildlife damage that exploits federal crop insurance data. We focused our study on four crops: corn, soybean, wheat, and cotton, chosen because of their economic importance and their vulnerability to wildlife damage.

RESULTS: We determined crop-raiding hot spots across the USA over the 2015–2019 period and identified the …

Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Marie Sutton

School of Biological Sciences: Dissertations, Theses, and Student Research

The Pestivirus genus contains several viral species having a major impact on the livestock species. While there were only four major pestiviral species for a time, recent metagenomic sequencing approaches identified additional species, such as atypical porcine pestivirus (APPV). Congenital tremor was first identified almost a hundred years ago and since still has an impact on swine health. There are two main types of congenital tremor, type A and B, with type A congenital tremor further subcategorized based on causative agent. Until recently, type A-II congenital tremor did not have a known cause. Recent viral sequencing of affected samples, including …

Evaluation Of Enrichment And Identification Media For Isolation Of Common Bovine Salmonella Serotypes, Katie Irene Swanson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salmonellosis is an important cause of diarrhea in humans and animals, which can lead to death in severe cases. Rapid and efficient detection of Salmonella spp. is essential to limit the spread of disease. Many enrichment procedures have been described for the isolation of Salmonella, and often include variations on Rappaport-Vassiliadis medium, which is recommended by the Food and Drug Administration (FDA). The current procedures vary in incubation temperature, incubation time, and type of media used for identification of Salmonella. This project compared four procedures for enrichment and identification of common bovine Salmonella serotypes. We determined the best …

Effects Of Freshwater Crayfish On Influenza A Virus Persistence In Water, J. Jeffrey Root, Jeremy W. Ellis, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

Several investigations have recently assessed the ability of some aquatic invertebrates to act as tools for avian influenza A virus (IAV) surveillance as well as their potential role(s) in IAV ecology. Because of this, as well as the high IAV seroprevalence rates noted in select mesocarnivores that commonly inhabit aquatic and semi-aquatic habitats, we evaluated the effects that freshwater crayfish have on IAV in water at three dose levels and monitored for the presence of IAV in crayfish tissues (gill and green gland) and haemolymph at multiple time points. At relatively high, medium 432 and low (approximately 10 , 10 …

Brain Complexity, Sentience And Welfare, Donald M. Broom

Animal Sentience

Neither sentience nor moral standing is confined to animals with large or human-like brains. Invertebrates deserve moral consideration. Definition of terms clarifies the relationship between sentience and welfare. All animals have welfare but humans give more protection to sentient animals. Humans should be less human-centred.

Spatiotemporal Genetic Diversity Of Lions Reveals The Influence Of Habitat Fragmentation Across Africa, Caitlin J. Curry, Brian W. Davis, Laura D. Bertola, Paula A. White, William J. Murphy, James N. Derr

Publications and Research

Direct comparisons between historical and contemporary populations allow for detecting changes in genetic diversity through time and assessment of the impact of habitat fragmentation. Here, we determined the genetic architecture of both historical and modern lions to document changes in genetic diversity over the last century. We surveyed microsatellite and mitochondrial genome variation from 143 high-quality museum specimens of known provenance, allowing us to directly compare this information with data from several recently published nuclear and mitochondrial studies. Our results provide evidence for male-mediated gene flow and recent isolation of local subpopulations, likely due to habitat fragmentation. Nuclear markers showed …

An Applied Ecology Of Fear Framework: Linking Theory To Conservation Practice, Kaitlyn M. Gaynor, Michael J. Cherry, Sophie L. Gilbert, Michael T. Kohl, Courtney L. Larson, Thomas M. Newsome, Laura R. Prugh, Justin P. Suraci, Julie K. Young, Justine A. Smith

United States Department of Agriculture Wildlife Services: Staff Publications

Research on the ecology of fear has highlighted the importance of perceived risk from predators and humans in shaping animal behavior and physiology, with potential demographic and ecosystem-wide consequences. Despite recent conceptual advances and potential management implications of the ecology of fear, theory and conservation practices have rarely been linked. Many challenges in animal conservation may be alleviated by actively harnessing or compensating for risk perception and risk avoidance behavior in wild animal populations. Integration of the ecology of fear into conservation and management practice can contribute to the recovery of threatened populations, human–wildlife conflict mitigation, invasive species management, maintenance …