The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, 2011 University of Tennessee - Knoxville
The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii
Animal Science Publications and Other Works
Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.
The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, 2011 University of Tennessee - Knoxville
The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii
Brian K Whitlock, PhD, DVM, DACT
Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.
The Effect Of Midazolam On End-Tidal Concentration Of Isoflurane Necessary To Prevent Movements In Dogs, 2011 University of Tennessee - Knoxville
The Effect Of Midazolam On End-Tidal Concentration Of Isoflurane Necessary To Prevent Movements In Dogs, Reza Seddighi Dvm, Ms, Phd, Dip Acva
Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences
Objective To determine the possible additive effect of midazolam, a GABAA agonist, on the end-tidal concentration of isoflurane that prevents movement (MACNM) in response to noxious stimulation. Study design Randomized crossover experimental study. Animals Six, healthy, adult intact male, mixed-breed dogs. Methods After baseline isoflurane MACNM (MACNM-B) determination, midazolam was administered as a low (LDS), medium (MDS) or high (HDS) dose series of midazolam. Each series consisted of two dose levels, low and high. The LDS was a loading dose (Ld) of 0.2 mg kg-1 and CRI (2.5 μg kg-1 minute-1) (LDL), followed by an Ld (0.4 mg kg-1) and …
The Effect Of Midazolam On End-Tidal Concentration Of Isoflurane Necessary To Prevent Movements In Dogs, 2011 University of Tennessee - Knoxville
The Effect Of Midazolam On End-Tidal Concentration Of Isoflurane Necessary To Prevent Movements In Dogs, Reza Seddighi Dvm, Ms, Phd, Dip Acva
Reza Seddighi
Objective To determine the possible additive effect of midazolam, a GABAA agonist, on the end-tidal concentration of isoflurane that prevents movement (MACNM) in response to noxious stimulation. Study design Randomized crossover experimental study. Animals Six, healthy, adult intact male, mixed-breed dogs. Methods After baseline isoflurane MACNM (MACNM-B) determination, midazolam was administered as a low (LDS), medium (MDS) or high (HDS) dose series of midazolam. Each series consisted of two dose levels, low and high. The LDS was a loading dose (Ld) of 0.2 mg kg-1 and CRI (2.5 μg kg-1 minute-1) (LDL), followed by an Ld (0.4 mg kg-1) and …
Estudio De Caso Uveítis En Equinos, 2011 Universidad de La Salle, Bogotá
Estudio De Caso Uveítis En Equinos, Angela Sofía Daniels Bazante
Medicina Veterinaria
No abstract provided.
Evaluación De La Influencia Del Periodo Estral De Oviductos Ovinos Usados En El Cultivo In Vivo, En La Calidad De Los Embriones Bovinos Fase 1, 2011 Universidad de La Salle, Bogotá
Evaluación De La Influencia Del Periodo Estral De Oviductos Ovinos Usados En El Cultivo In Vivo, En La Calidad De Los Embriones Bovinos Fase 1, Caroline Nieto Duque
Medicina Veterinaria
Se evalúo si el estadio del ciclo estral ovino, afecta la calidad del embrión bovino al colectarlos al séptimo día después de haber sido cultivados; El experimento se realizo utilizando los oviductos ovinos como medio de cultivo in vivo, se tuvieron en cuenta dos grupos diferentes, en el grupo A, las hembras ovinas se encontraban en estro por medio de la previa sincronización de las mismas, y el grupo B, se encontraban en diestro, sincronizando este grupo 10 días antes, lo que garantiza que se encuentren en una fase luteal, el grupo control fue los embriones del grupo B. Al …
Efecto De Dos Fuentes De Progesterona Exógena, Sobre La Ovulación Cantidad De Folículos Ovulados En Vacas Criollas Sanmartineras, Sobrestimuladas Con Fsh-P Lh-P Pluset, 2011 Universidad de La Salle, Bogotá
Efecto De Dos Fuentes De Progesterona Exógena, Sobre La Ovulación Cantidad De Folículos Ovulados En Vacas Criollas Sanmartineras, Sobrestimuladas Con Fsh-P Lh-P Pluset, Mauricio González Leal
Medicina Veterinaria
El objetivo de este trabajo fue evaluar el efecto de dos fuentes de progesterona exógena de origen natural (CIDR® ) y de origen sintético (Crestar® ), en la ovulación de hembras bovinas de la raza Sanmartinero, superestimuladas con FSH-p y LH-p (PLUSET® ), se utilizaron 6 vacas de la raza Sanmartinero distribuidas en dos tratamientos: 1) 3 vacas con 1 implante auricular (CRESTAR® Lab. Intervet) impregnado con 3mg de Norgestomet mas 2.5 mg Benzoato de estradiol (Estrozoo® Lab. ZOO) y 50 mg de progesterona (Gestavec-25 ® Lab. Vecol) en el día 0; 2) 3 vacas con dispositivo intravaginal (CIDR® Lab. …
Reporte De Casos De Intolerancia Al Ejercicio En Caballos De Salto En La Sabana De Bogotá, 2011 Universidad de La Salle, Bogotá
Reporte De Casos De Intolerancia Al Ejercicio En Caballos De Salto En La Sabana De Bogotá, Mariana Zuluaga Ballesteros
Medicina Veterinaria
Teniendo en cuenta que el bajo rendimiento deportivo aparece reportado como una de las causas de consulta más frecuentes en los equinos atletas, surge la inquietud y la necesidad de investigar las patologías de vías aéreas superiores e inferiores que generan intolerancia al ejercicio, y su presentación en la Sabana de Bogotá. Se debe tener presente que las afecciones del sistema respiratorio aparecen reportadas como la segunda causa de intolerancia al ejercicio, después del sistema musculoesquelético, en caballos de alto desempeño a nivel mundial. Este estudio fue realizado con equinos de la modalidad de salto en competencia, cuya causa de …
Regulation Of The Latency–Reactivation Cycle By Products Encoded By The Bovine Herpesvirus 1 (Bhv-1) Latency-Related Gene, 2011 University of Nebraska-Lincoln
Regulation Of The Latency–Reactivation Cycle By Products Encoded By The Bovine Herpesvirus 1 (Bhv-1) Latency-Related Gene, Clinton Jones, Leticia Frizzo Da Silva, Devis Sinani
School of Veterinary and Biomedical Sciences: Faculty Publications
Like other α-herpesvirinae subfamily members, the primary site for bovine herpesvirus 1 (BHV-1) latency is ganglionic sensory neurons. Periodically BHV-1 reactivates from latency, virus is shed, and consequently virus transmission occurs. Transcription from the latency-related (LR) gene is readily detected in neurons of trigeminal ganglia (TG) of calves or rabbits latently infected with BHV-1. Two micro-RNAs and a transcript encompassing a small open reading frame (ORF-E) located within the LR promoter can also be detected in TG of latently infected calves. A BHV-1 mutant that contains stop codons near the beginning of the first open reading frame (ORF2) within the …
Caring For Compromised Cattle: Assessing Animals At Risk, 2011 University of Nebraska - Lincoln
Caring For Compromised Cattle: Assessing Animals At Risk
Nebraska Beef Quality Assurance Program
Cattlemen have long recognized the need to properly care for their livestock. Sound animal husbandry practices – based on research and decades of practical experience – are known to impact the well-being of cattle, individual animal health and herd productivity. There is no doubt that producers work hard to ensure that their cattle are properly cared for throughout all stages of production. Unfortunately, it’s a fact of raising cattle that some animals will become injured to the extent that they are unable to stand or walk without assistance.
Assessor’S Guide To A Beef Quality Assurance Cow-Calf Assessment, 2011 University of Nebraska - Lincoln
Assessor’S Guide To A Beef Quality Assurance Cow-Calf Assessment
Nebraska Beef Quality Assurance Program
The BQA Cow-Calf Assessment is an on-site educational tool that allows for assessing and benchmarking key indicators of animal care and well-being as well as operational conditions. The Cow-Calf Assessment focuses on three main areas – Animals, Records and Best Management Practices (BMP), and Facilities and Equipment.
Efecto Del Medio Extensor Y De Las Curvas De Enfriamiento Y Congelamiento En La Célula Espermática Equina En Caballos De La Sabana De Bogotá, 2011 Universidad de La Salle, Bogotá
Efecto Del Medio Extensor Y De Las Curvas De Enfriamiento Y Congelamiento En La Célula Espermática Equina En Caballos De La Sabana De Bogotá, Paula Ospitia Rodríguez, Alexandra González Caicedo
Medicina Veterinaria
Este trabajo tiene como objetivo evaluar el efecto de dos crioprotectores en el medio de congelación INRA 82 modificado con azúcares, asociados a las curvas de enfriamiento y congelamiento sobre la célula espermática de caballos criollos colombianos. Un eyaculado de seis caballos fue utilizado para evaluar los parámetros de motilidad, vigor, morfología e integridad de membrana plasmática; cada eyaculado se dividió en dos alícuotas iguales al azar con variaciones en la adición del crioprotector (DMF 5% o Glicerol 5%) y se congelo siguiendo el mismo protocolo de criopreservación, con una curva de enfriamiento de 5ºC durante 120 minutos y exposición …
Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb, 2011 Michigan State University
Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb, A. F. Lehner, J. A. Hitron, J. May, C. Hughes, R. Eisenberg, N. Schwint, D. P. Knowles, P. Timoney, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
Imidocarb [N,N'-bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea, C19H20N6O1, m.w. 348.41] is a symmetrical carbanilide derivative used to treat disease caused by protozoans of the Babesia genus. Imidocarb, however, is also considered capable of suppressing Babesia-specific immune responses, allowing Babesia-positive horses to pass a complement fixation test (CFT) without eliminating the infection. This scenario could enable Babesia-infected horses to pass CFT-based importation tests. It is imperative to unequivocally identify and quantify equine tissue residues of imidocarb by mass spectrometry to address this issue. As a pretext to development of sensitive tissue assays, we have investigated possibilities of mass …
Diagnosis And Management Of Horses With Equine Metabolic Syndrome (Ems), 2010 University of Tennessee, Knoxville
Diagnosis And Management Of Horses With Equine Metabolic Syndrome (Ems), Kelly Ann Chameroy
Doctoral Dissertations
In horses, a painful and often debilitating disease known as laminitis can result in impaired function and, in severe cases, euthanasia. Equine Metabolic Syndrome (EMS) is a syndrome in horses that results in development of laminitis and is characterized by the presence of general and/or regional adiposity (“cresty neck”), aberrations in blood lipid concentrations, insulin resistance (IR) and/ or hyperinsulinemia. Therapies have focused on improving the state of obesity and insulin resistance with the goal of diminishing the likelihood of laminitis development. A definitive cause for laminitis has not been established, but hyperinsulinemia and IR are likely candidates as experimental …
Effects Of Pituitary Pars Intermedia Dysfunction (Ppid), Season, And Pasture Diet On Blood Adrenocorticotropic Hormone And Metabolite Concentrations In Horses., 2010 University of Tennessee - Knoxville
Effects Of Pituitary Pars Intermedia Dysfunction (Ppid), Season, And Pasture Diet On Blood Adrenocorticotropic Hormone And Metabolite Concentrations In Horses., Sarah Beth Elliott
Masters Theses
Studies described in this thesis were performed to investigate associations among season, diet, pituitary pars intermedia dysfunction (PPID) and blood concentrations of adrenocorticotropic hormone (ACTH), insulin, glucose, and leptin in horses. In the first study, higher ACTH concentrations were detected in horses affected with PPID. A seasonal increase in plasma ACTH concentration was detected in the late summer and early fall, but PPID did not affect the timing or duration of this increase. Pasture grazing raised glucose and insulin concentrations with a peak in September, at the same time that horses had higher ACTH concentrations, and this convergence of risk …
Local Anesthetics As Pain Therapy In Horses, 2010 University of Tennessee - Knoxville
Local Anesthetics As Pain Therapy In Horses, Reza Seddighi Dvm, Ms, Phd, Dip Acva
Faculty Publications and Other Works -- Large Animal Clinical Sciences
Local Anesthetics as Pain Therapy in Horses Thomas J. Doherty MVB, MSc, and M. Reza Seddighi DVM, PhD
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, The University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA
This article describes the rationale behind the use of systemically administered lidocaine as an analgesic. The analgesic efficacy of intravenously administered lidocaine is well documented by studies in human patients and laboratory animals. The mechanism by which systemically administered lidocaine produces analgesia is uncertain but is thought to include action at sodium, calcium, and potassium channels and the N-methyl-D-aspartate acid receptor. …
Local Anesthetics As Pain Therapy In Horses, 2010 University of Tennessee - Knoxville
Local Anesthetics As Pain Therapy In Horses, Reza Seddighi Dvm, Ms, Phd, Dip Acva
Reza Seddighi
Local Anesthetics as Pain Therapy in Horses Thomas J. Doherty MVB, MSc, and M. Reza Seddighi DVM, PhD
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, The University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA
This article describes the rationale behind the use of systemically administered lidocaine as an analgesic. The analgesic efficacy of intravenously administered lidocaine is well documented by studies in human patients and laboratory animals. The mechanism by which systemically administered lidocaine produces analgesia is uncertain but is thought to include action at sodium, calcium, and potassium channels and the N-methyl-D-aspartate acid receptor. …
Selected Hormonal And Neurotransmitter Mechanisms Regulating, 2010 Auburn University Main Campus
Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn
Faculty Publications and Other Works -- Large Animal Clinical Sciences
Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …
Selected Hormonal And Neurotransmitter Mechanisms Regulating, 2010 Auburn University Main Campus
Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn
Brian K Whitlock, PhD, DVM, DACT
Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …
Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, 2010 Berry College
Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, Joseph A. Daniel, A B. Milam, M E. Gafnea, Brian K. Whitlock, D H. Keisler
Animal Science Publications and Other Works
Previous research has demonstrated circulating concentrations of leptin increase in ewes during mid pregnancy then decline in late pregnancy and early lactation. The objective of this study was to more narrowly define the timing of changes in circulating concentrations of leptin with pregnancy in ewes. Katahdin ewes (n= 19) located at latitude 34.275 and longitude -85.183 (Mount Berry, GA) were utilized. Blood samples were collected weekly via jugular veinpuncture beginning immediately before ram exposure on September 23 and continuing until 4 weeks post-lambing. Ewes were exposed to a ram fitted with a marking harness for a 63 day breeding season. …