Discovery And Effects Of Pharmacological Inhibition Of The E3 Ligase Skp2 By Small Molecule Protein-Protein Interaction Disruptors,
2018
The University of Texas M D Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Discovery And Effects Of Pharmacological Inhibition Of The E3 Ligase Skp2 By Small Molecule Protein-Protein Interaction Disruptors, John K. Morrow
Dissertations and Theses (Open Access)
Skp2 (S-phase kinase-associated protein 2), one component of the SCF E3 ubiquitin ligase complex, directly interacts with Skp1 and indirectly associates with Cullin1 and Rbx1 to bridge the E2 conjugating enzyme with its protein substrate to execute its E3 ligase activity. Skp2 is an Fbox protein (due to it containing an Fbox domain) and it is the rate-limiting component of the SCF complex. Skp2 targets several cell-cycle regulatory proteins for ubiquitination and degradation; most notable and significant for cancer are the cyclin-dependent kinase inhibitor, p27. Skp2 is an oncogene and studies have shown that over-expression of Skp2 leads to increased …
The Pharmacogene Variation (Pharmvar) Consortium: Incorporation Of The Human Cytochrome P450 (Cyp) Allele Nomenclature Database,
2018
Children's Mercy Kansas City
The Pharmacogene Variation (Pharmvar) Consortium: Incorporation Of The Human Cytochrome P450 (Cyp) Allele Nomenclature Database, Andrea Gaedigk, Magnus Ingelman-Sundberg, Neil A. Miller, J Steven Leeder, Michelle Whirl-Carrillo, Teri E. Klein
Manuscripts, Articles, Book Chapters and Other Papers
The Human Cytochrome P450 (CYP) Allele Nomenclature Database, a critical resource to the pharmacogenetics and genomics communities, will be transitioning to the Pharmacogene Variation (PharmVar) Consortium. In this report we provide a summary of the current database, provide an overview of the PharmVar consortium and highlight the PharmVar database which will serve as the new home for pharmacogene nomenclature.
Microtubule-Actin Crosslinking Factor 1 And Plakins As Therapeutic Drug Targets,
2018
Tennessee State University
Microtubule-Actin Crosslinking Factor 1 And Plakins As Therapeutic Drug Targets, Quincy A. Quick
Biology Faculty Research
Plakins are a family of seven cytoskeletal cross-linker proteins (microtubule-actin crosslinking factor 1 (MACF), bullous pemphigoid antigen (BPAG1) desmoplakin, envoplakin, periplakin, plectin, epiplakin) that network the three major filaments that comprise the cytoskeleton. Plakins have been found to be involved in disorders and diseases of the skin, heart, nervous system, and cancer that are attributed to autoimmune responses and genetic alterations of these macromolecules. Despite their role and involvement across a spectrum of several diseases, there are no current drugs or pharmacological agents that specifically target the members of this protein family. On the contrary, microtubules have traditionally been targeted …
Loss Of The Intellectual Disability And Autism Gene Cc2d1a And Its Homolog Cc2d1b Differentially Affect Spatial Memory, Anxiety, And Hyperactivity,
2018
George Washington University
Loss Of The Intellectual Disability And Autism Gene Cc2d1a And Its Homolog Cc2d1b Differentially Affect Spatial Memory, Anxiety, And Hyperactivity, Marta Zamarbide, Adam Oaks, Heather Pond, Julia Adelman, M. Chiara Manzini
Pharmacology and Physiology Faculty Publications
Hundreds of genes are mutated in non-syndromic intellectual disability (ID) and autism spectrum disorder (ASD), with each gene often involved in only a handful of cases. Such heterogeneity can be daunting, but rare recessive loss of function (LOF) mutations can be a good starting point to provide insight into the mechanisms of neurodevelopmental disease. Biallelic LOF mutations in the signaling scaffold CC2D1Acause a rare form of autosomal recessive ID, sometimes associated with ASD and seizures. In parallel, we recently reported that Cc2d1a-deficient mice present with cognitive and social deficits, hyperactivity and anxiety. In Drosophila, loss of the only …
Optimization Of Wavelength Selection For Multispectral Image Acquisition: A Case Study Of Atrial Ablation Lesions,
2018
George Washington University
Optimization Of Wavelength Selection For Multispectral Image Acquisition: A Case Study Of Atrial Ablation Lesions, Huda Asfour, S. Guan, Narine Muselimyan, Luther M. Swift, Murray Loew, Narine Sarvazyan
Pharmacology and Physiology Faculty Publications
In vivo autofluorescence hyperspectral imaging of moving objects can be challenging due to motion artifacts and to the limited amount of acquired photons. To address both limitations, we selectively reduced the number of spectral bands while maintaining accurate target identification. Several downsampling approaches were applied to data obtained from the atrial tissue of adult pigs with sites of radiofrequency ablation lesions. Standard image qualifiers such as the mean square error, the peak signal-to-noise ratio, the structural similarity index map, and an accuracy index of lesion component images were used to quantify the effects of spectral binning, an increased spectral distance …
Dysregulation Of Daf-16/Foxo3a-Mediated Stress Responses Accelerates T Oxidative Dna Damage Induced Aging,
2018
The Scripps Research Institute
Dysregulation Of Daf-16/Foxo3a-Mediated Stress Responses Accelerates T Oxidative Dna Damage Induced Aging, Aditi U. Gurkar, Andria R. Robinson, Yuxiang Cui, Xuesen Li, Shailaja K. Allani, Amanda Webster, Mariya Muravia, Mohammad Fallahi, Herbert Weissbach, Paul D. Robbins, Yinsheng Wang, Eric E. Kelley, Claudette M. St. Croix, Laura J. Niedernhofer, Matthew S. Gill
Faculty & Staff Scholarship
DNA damage is presumed to be one type of stochastic macromolecular damage that contributes to aging, yet little is known about the precise mechanism by which DNA damage drives aging. Here, we attempt to address this gap in knowledge using DNA repair-deficient C. elegans and mice. ERCC1-XPF is a nuclear endonuclease required for genomic stability and loss of ERCC1 in humans and mice accelerates the incidence of age-related pathologies. Like mice, ercc-1 worms are UV sensitive, shorter lived, display premature functional decline and they accumulate spontaneous oxidative DNA lesions (cyclopurines) more rapidly than wild-type worms. We found that ercc-1 worms …
Investigation Of Association Of Chemical Profiles With The Tracheobronchial Relaxant Activity Of Chinese Medicinal Herb Beimu Derived From Various Fritillaria Species.,
2018
Southwest Medical University
Investigation Of Association Of Chemical Profiles With The Tracheobronchial Relaxant Activity Of Chinese Medicinal Herb Beimu Derived From Various Fritillaria Species., Xu Wu, Shun-Wan Chan, Jiang Ma, Ping Li, Pang-Chui Shaw, Ge Lin
Faculty of Science & Technology (THEi)
Ethnopharmacological relevance Fritillariae Bulbus (Beimu in Chinese) is derived from the bulbus of many Fritillaria species (family Liliaceae), which has been used as an antitussive herb in traditional Chinese medicine for more than 2000 years. Due to the complexity of plant origins and significant variations in chemical profiles, the characterization of the profile of the major bioactive constituents and its association with pharmacological activity are important for the quality control of Beimu herbs from different origins. Aim of the study This study aims to investigate the distribution of major bioactive isosteroidal alkaloids in Beimu herbs of different origins and its …
Rhythm: An Open Source Imaging Toolkit For Cardiac Panoramic Optical Mapping,
2018
George Washington University
Rhythm: An Open Source Imaging Toolkit For Cardiac Panoramic Optical Mapping, Christopher Gloschat, Kedar Aras, Shubnam Gupta, N. Rokhana Faye, Hanyu Zhang, Matthew W. Kay, Igor R Efimov, +Several Additional Authors
Pharmacology and Physiology Faculty Publications
Fluorescence optical imaging techniques have revolutionized the field of cardiac electrophysiology and advanced our understanding of complex electrical activities such as arrhythmias. However, traditional monocular optical mapping systems, despite having high spatial resolution, are restricted to a two-dimensional (2D) field of view. Consequently, tracking complex three-dimensional (3D) electrical waves such as during ventricular fibrillation is challenging as the waves rapidly move in and out of the field of view. This problem has been solved by panoramic imaging which uses multiple cameras to measure the electrical activity from the entire epicardial surface. However, the diverse engineering skill set and substantial resource …
A Novel Assay For Evaluation Of The Total Antioxidant Capacity (Tac) Using A Nontoxic Probe,
2018
Chulalongkorn University
A Novel Assay For Evaluation Of The Total Antioxidant Capacity (Tac) Using A Nontoxic Probe, Sirirat Panich
The Thai Journal of Pharmaceutical Sciences
A novel and simple method for measuring total antioxidant capacity (TAC) was established. Herein, the new assay is based on generating the deep blue Imipramine (IMI) free radical by oxidising IMI with ammonium tetra vanadate (NH4VO3) in acid medium. Once.
In Silico Evaluation Of Molecular Interactions Between Known ?-Glucosidase Inhibitors And Homologous ?-Glucosidase Enzymes From Saccharomyces Cerevisiae, Rattus Norvegicus, And Ganc-Human,
2018
Chulalongkorn University
In Silico Evaluation Of Molecular Interactions Between Known ?-Glucosidase Inhibitors And Homologous ?-Glucosidase Enzymes From Saccharomyces Cerevisiae, Rattus Norvegicus, And Ganc-Human, Teni Ernawati
The Thai Journal of Pharmaceutical Sciences
The aim of this study was to observe molecular interactions between alpha glucosidase inhibitor waith alpha glucosidase enzyme derived from Saccharomyces cereviseae, Rattus norvegicus and GANC-human. Therefore, they were used against four of the well-kno.
Intended High-Performance Liquid Chromatography Procedure For The Quantification Of Norfloxacin And Its Potential Impurities In Active Pharmaceutical Ingredient And Tablet Dosage Forms,
2018
Chulalongkorn University
Intended High-Performance Liquid Chromatography Procedure For The Quantification Of Norfloxacin And Its Potential Impurities In Active Pharmaceutical Ingredient And Tablet Dosage Forms, Bikshal Babu Kasimala, Venkateswara Rao Anna, Useni Reddy Mallu, L. Maheshwara Reddy
The Thai Journal of Pharmaceutical Sciences
Norfloxacin [NFXC] is an active pharmaceutical ingredient belongs to 4-quinolone antibacterial agent used for the prophylaxis of gram +ve and gram-ve bacterial infections. A reliable stability-indicating RP-HPLC method was developed for the separation an.
Development And Validation Of Novel Stability-Indicating High-Performance Liquid Chromatography Method For The Determination Of Miglitol In Pharmaceuticals,
2018
Chulalongkorn University
Development And Validation Of Novel Stability-Indicating High-Performance Liquid Chromatography Method For The Determination Of Miglitol In Pharmaceuticals, Kanakapura Basavaiah, Nagaraju Rajendraprasad
The Thai Journal of Pharmaceutical Sciences
A reversed phase high performance liquid chromatographic (RP-HPLC) method for the determination of miglitol (MGL) in pharmaceuticals is presented. The assay was performed on a Phenomenex Luna C18 (150 × 4.6 mm; 5µm particle size) column using phosphate...
Quantitative Structure–Activity Relationship Modeling Of S-Triazines And 2-Arylpyrimidines As Selective Pde4b Inhibitors,
2018
Chulalongkorn University
Quantitative Structure–Activity Relationship Modeling Of S-Triazines And 2-Arylpyrimidines As Selective Pde4b Inhibitors, Anand Gaurav, Gabriel Akyirem Akowuah, Choo Shiuan Por
The Thai Journal of Pharmaceutical Sciences
A QSAR analysis was conducted on a series of 2-arylpyrimidine and s-triazine derivatives as selective PDE4B inhibitors. Primary objective of the study is to develop predictive QSAR models for s-triazines and 2-arylpyrimidines as selective PDE4B and to id.
Ethical Cases On Pharmacy Profession Practice In Thailand During A.D. 1995 To 2015,
2018
Chulalongkorn University
Ethical Cases On Pharmacy Profession Practice In Thailand During A.D. 1995 To 2015, Wanna Sriviriyanupap, Usawadee Sutapuk
The Thai Journal of Pharmaceutical Sciences
Background: Code of ethics for the pharmacy profession was established to enforce all pharmacists to abide by a rule for a good pharmacy professional practice. Objective: to study the ethical problems, related to the pharmacy profession practice, and to .
Population Pharmacokinetics Analysis Of Vancomycin In Critically Ill Patients,
2018
Chulalongkorn University
Population Pharmacokinetics Analysis Of Vancomycin In Critically Ill Patients, Wanchai Treyaprasert
The Thai Journal of Pharmaceutical Sciences
Objectives: The aim of this study was to determine population pharmacokinetic parameters of vancomycin in adult critically ill patients and to investigate the covariates affecting population pharmacokinetic parameters. Methods: Plasma concentration data..
Effect Of Temperature And Time On Free Amino Acid Profile In Thai Chicken Bone Soup Stock Preparation,
2018
Chulalongkorn University
Effect Of Temperature And Time On Free Amino Acid Profile In Thai Chicken Bone Soup Stock Preparation, Nattida Chotechuang, Matichon Lokkhumlue, Pawinee Deetae
The Thai Journal of Pharmaceutical Sciences
Soup stock is the basic material for cooking various types of food, including Thai food. General practice to prepare Thai soup stock is to gentle boil chicken bone soup stock between 85-95°C for 1-3 hours to get good quality soup stock. Free amino aci..
Apigenin And Baicalin, Each Alone Or In Low Dose Combination, Attenuated Chloroquine Induced Male Infertility In Adult Rats,
2018
Chulalongkorn University
Apigenin And Baicalin, Each Alone Or In Low Dose Combination, Attenuated Chloroquine Induced Male Infertility In Adult Rats, Amira Akilah, Mohamed Balaha, Mohamed-Nabeih Abd-El Rahman, Sabiha Hedya
The Thai Journal of Pharmaceutical Sciences
Male infertility is a worldwide health problem, which accounts for about 50% of all cases of infertility and considered as the commonest single defined cause of infertility. Recently, apigenin and baicalin exhibited a powerful antioxidant and antiapoptot.
The Protective Effect Of Lycopene-Rich Products On Skin Photodamage: A Systematic Review And Meta-Analysis Of Randomized Controlled Trials,
2018
Chulalongkorn University
The Protective Effect Of Lycopene-Rich Products On Skin Photodamage: A Systematic Review And Meta-Analysis Of Randomized Controlled Trials, Witoo Dilokthornsakul, Teerapon Dhippayom, Piyameth Dilokthornsakul
The Thai Journal of Pharmaceutical Sciences
Background: Ultraviolet (UV) radiation has known as a major cause of photodamage, photoaging and skin cancer as it involves in reactive oxygen species generation. Several natural antioxidants including lycopene has been suggested for photoprotection. How.
Suitability Of Alternative Atp Potency Assay For Lot Release Of Tokyo Bcg- 172-1 Vaccines In Thailand,
2018
Chulalongkorn University
Suitability Of Alternative Atp Potency Assay For Lot Release Of Tokyo Bcg- 172-1 Vaccines In Thailand, Supaporn Phumiamorn
The Thai Journal of Pharmaceutical Sciences
The potency assay of the BCG vaccine is routinely performed by a conventional viable count on a solid medium. This assay method is not only time-consuming, but can give variable results because of the tendency to clump of mycobacterium. The intracellular.
A Simple High-Performance Liquid Chromatographic Method For Quantitative Analysis Of Brazilin In Caesalpinia Sappan L. Extracts,
2018
Chulalongkorn University
A Simple High-Performance Liquid Chromatographic Method For Quantitative Analysis Of Brazilin In Caesalpinia Sappan L. Extracts, Sakan Warinhomhaun, Boonchoo Sritularak, Dusadee Charnvanich
The Thai Journal of Pharmaceutical Sciences
The specific aim of the study was to develop and validate a simple reversed-phase high-performance liquid chromatographic (HPLC) method for assay of brazilin in Caesalpinia sappan L. (sappanwood) extracts. Validation of the HPLC method was conducted unde.
