Untangling The Roles Of Pulmonary Vagal Afferent Subsets With Inhaled Irritants,
2025
University of South Florida
Untangling The Roles Of Pulmonary Vagal Afferent Subsets With Inhaled Irritants, Teresa Darcey
USF Tampa Graduate Theses and Dissertations
The sensory nerves in the airways directly regulate critical defenses and indirectly regulate vital gas exchange. Airway function is largely controlled by the autonomic nervous system, and our understanding of its sensory regulation is directly connected to our ability to treat respiratory and cardiovascular pathologies. Airway sensory afferents are heterogeneous with respect to their electrophysiological properties, peptidergic capacity, and stimuli sensitivity, which at least in part dictate some of their various functions. There is evidence that embryological origin impacts reflex function: activation of the neural crest-derived population of sensory afferents has been shown to evoke the cough reflex whereas activation …
Development And Validation Of An Analytical Method For Determination Of Bongkrekic Acid In Biofluids For Toxin Monitoring,
2025
Forensic and Clinical Toxicology Center, National Taiwan University Hospital, Taipei, Taiwan
Development And Validation Of An Analytical Method For Determination Of Bongkrekic Acid In Biofluids For Toxin Monitoring, Ying-Tzu Shieh, Te-I Weng, Ju-Yu Chen, I-Ting Wang, Kevin Shu-Leung Lai, Chia-Mo Lin, Chu-Yun Teng, Guan-Yuan Chen
Journal of Food and Drug Analysis
Bongkrekic acid (BKA) poisoning is a severe foodborne illness with a high mortality rate. This study aimed to identifyBKA in postmortem tissues from poisoning victims in Taiwan and to develop a reliable analytical method for detectingBKA in biofluids to aid clinical diagnosis and treatment. BKA was identified in postmortem samples using high-reso-lution mass spectrometry (HRMS) and confirmed with an independent ultrahigh-performance liquid chromatographycoupled with tandem mass spectrometry (UHPLC-MS/MS) method. To meet clinical needs, a UHPLC-MS/MS methodwas developed and validated for BKA detection in plasma and urine. Method optimization included adjustments to ionsource conditions for multiple reaction monitoring (MRM) transitions and …
The Economic Burden Of Covid-19 In A Region With Stringent Response Measures: A Case Study Of Taiwan,
2025
Department of Health Service Administration, College of Public Health, China Medical University, Taichung, Taiwan
The Economic Burden Of Covid-19 In A Region With Stringent Response Measures: A Case Study Of Taiwan, Elise Chia-Hui Tan, Fei-Yuan Hsiao, Ming-Chin Yang
Journal of Food and Drug Analysis
The COVID-19 pandemic has imposed a significant economic burden globally, particularly in regions with stringentresponse measures. This study aimed to assess the economic impact of COVID-19 in Taiwan, focusing on both directand indirect costs. A cost-of-illness analysis was conducted, utilizing data from the Taiwan Centers for Disease Control(CDC), national databases, epidemiological studies, and economic surveys. The analysis included both direct costs (e.g.,hospital admissions, outpatient care) and indirect costs (e.g., productivity losses due to long COVID, absenteeism, caregiving duties). The study encompassed Taiwan's population of 23.2 million, with particular attention to age-specific impacts on economic outcomes. The total economic burden of …
Nanovesicles Derived From Protocorm-Like Bodies Of Dendrobium Officinal Enhance Corneal Epithelial Repair And Reduce Inflammation In Dry Eye Disease Models,
2025
Jinzhou Medical University
Nanovesicles Derived From Protocorm-Like Bodies Of Dendrobium Officinal Enhance Corneal Epithelial Repair And Reduce Inflammation In Dry Eye Disease Models, Bao Yuqing, Peng Chengyu, Zhang Fenglei, Zheng Yuqiang, Zhang Mingqi, He Wei
Journal of Food and Drug Analysis
Plant-derived nanovesicles (PDNVs) offer a promising, biocompatible therapeutic platform. In this study, we isolatednanovesicles from protocorm-like bodies of Dendrobium officinale (PLBs-NVs) and evaluated their efficacy in dry eyedisease (DED) models. PLBs-NVs protected human corneal epithelial cells against hyperosmotic stress, supportedwound repair, and inhibited inflammation in vitro. In a desiccating stress-induced DED mouse model, topical PLBs-NVstreatment significantly restored tear secretion, reduced corneal epithelial damage, and lowered levels of proinflammatorycytokines. These results suggest that PLBs-NVs are a safe, natural, and effective therapeutic strategy for DED, sup-porting their potential for future clinical translation in ocular surface disorders.
Protective Effects Of Jujube Seed Extract Against Oxidative Stress In Ht22 Cells And Its Sleep-Promoting Action Through Gabaergic Receptors,
2025
Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Republic of Korea
Protective Effects Of Jujube Seed Extract Against Oxidative Stress In Ht22 Cells And Its Sleep-Promoting Action Through Gabaergic Receptors, Seok Hyun Chung, Hyeongyeong Kim, Singeun Kim, Pan-Young Jeong, Hyowon Lee
Journal of Food and Drug Analysis
Jujube seed extract (JSE), rich in polyphenols and flavonoids, exhibits neuroprotective and sleep-enhancing properties.In vitro, JSE protected HT22 cells against oxidative stress by activating the Nrf2/HO-1 pathway and upregulatingantioxidant enzymes. In vivo, JSE increased non-REM sleep and delta wave activity in pentobarbital- and caffeine-induced sleep models. Spinosin contributed to these effects. Long-term JSE administration upregulated GABAA, GABAB, and 5-HT1A receptors in brain tissue. These findings suggest that JSE offers dual benefits in mitigating oxidativestress and promoting sleep through antioxidant defense and neurotransmitter modulation, supporting its potential as a natural therapeutic agent.
Repurposing Dihydroartemisinin As A Novel Anticancer Agent Against Colorectal Cancer Stem Cells,
2025
School of Pharmacy, National Taiwan University, Taipei 10050, Taiwan
Repurposing Dihydroartemisinin As A Novel Anticancer Agent Against Colorectal Cancer Stem Cells, Meng-Han Wu, Chieh-Ju Sung, Fan-Lu Kung, Jih-Hwa Guh, Yu Su, Lih-Ching Hsu
Journal of Food and Drug Analysis
Colorectal cancer (CRC) is one of the leading causes of cancer-related death globally and discovering novel therapeuticagents to treat the disease, and prevent cancer metastasis and recurrence is an urgent medical need. Cancer stem cells(CSCs) capable of self-renewal and differentiation are generally considered the cause of tumor metastasis, recurrenceand chemoresistance. Hence, targeting CSCs may be a promising strategy for the treatment of cancer. GATA6, a zincfinger transcription factor, contributes to tumorigenesis in CRC and is related to cancer stemness. GATA6-overexpressing stable clones OE4 and OE6 derived from HCT116 cells were previously established and exhibitedincreased stemness properties. In this study, we …
Yi-Qi-Xuan-Fei Formula Ameliorate Chronic Obstructive Pulmonary Disease By Remodeling Lung And Intestinal Florase In Rat Models,
2025
Yunnan Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease Prevention and Control, Yunnan University of Chinese Medicine, Kunming,650500, China
Yi-Qi-Xuan-Fei Formula Ameliorate Chronic Obstructive Pulmonary Disease By Remodeling Lung And Intestinal Florase In Rat Models, Si-Si Han, Li-Yun Song, Peng-Tao Liang, Yin-Ying Wang, Yi Ying, Li Li, Jia-Li Yuan, Zhong-Shan Yang
Journal of Food and Drug Analysis
Chronic obstructive pulmonary disease (COPD) is a chronic respiratory disease characterized by various pathologicallesions and an imbalance in the microflora. The Yi-Qi-Xuan-Fei Formula (YQXF) is a clinically effective formula withpharmacological potential to delay the progression of COPD. This study aims to explore the relationship between thetherapeutic mechanism of YQXF and the microflora in COPD. Our study found that YQXF reduces inflammatory injuryand inflammatory cell infiltration in lung tissue, repairs the intestinal mucosal barrier, and enhances immune function.Additionally, YQXF regulates the pulmonary and intestinal flora by increasing the abundance of Alloprevotella, Rose-buria, Oscillibacter, and Lactobacillus, while reducing the abundance of …
Precision Nanomedicine For Anxiety: Challenges, Opportunities, And Future Directions In Targeted Drug Delivery,
2025
Department of Pharmaceutics, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Bela, Ropar (140111) Punjab, India
Precision Nanomedicine For Anxiety: Challenges, Opportunities, And Future Directions In Targeted Drug Delivery, Sehrabpreet Singh, Amanpreet Singh, Shruti Chittu, Shailesh Sharma
Journal of Food and Drug Analysis
Nanoparticle-based drug delivery system represents one of the challenging strategies suggested to improve anxietydisorder therapeutic approaches, clinic challenges of delayed action, side effect-free designing and poor patientcompliance. Traditional pharmacological agents can increase drug bioavailability and target specific brain regions,whereas nanoparticle-mediated controlled release offers enhanced precision and sustained action. The others in thisreview concentrate on several kinds of nanoparticle, including lipid-based, polymeric-metallic, and responsive nano-particles, their use in anxiety medication. In addition, emphasis is placed on precision medicine that pertain the delivery of treatment based on an individual's genetic, environmental and lifestyle aspects. Also, it is looked into how artificial intelligence …
The Rhythm Of Decline: Circadian Disruption In Neurodegeneration,
2025
Department of pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Bela, Ropar, Punjab, India, PIN: 140111
The Rhythm Of Decline: Circadian Disruption In Neurodegeneration, Jeewanjot Singh, Devinder Kumar, Jasleen Kaur, Amanpreet Singh
Journal of Food and Drug Analysis
Alzheimer's disease (AD) is a progressive neurodegenerative disorder with a multifactorial etiology involving genetic,environmental, and metabolic factors. Among these, circadian rhythm disruption has emerged as a crucial but under-explored contributor to disease progression. The circadian system, regulated by the suprachiasmatic nucleus (SCN),controls essential physiological functions such as the sleep-wake cycle, metabolism, and neuroendocrine signaling.Disruption of this system has been increasingly linked to key pathological features of AD, including amyloid-betaaccumulation, tau hyperphosphorylation, and neuroinflammation. This review critically examines the mechanistic roleof circadian misalignment in AD by analyzing studies on sleep disturbances, SCN degeneration, metabolic dysregu-lation, clock gene polymorphisms (BMAL1, CLOCK, PER, …
Targeted Nanosponge-Based Treatment For Pulmonary Hypertension: A Novel Approach,
2025
Department of pharmaceutics, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Bela, Ropar, Punjab, India, PIN: 140111,
Targeted Nanosponge-Based Treatment For Pulmonary Hypertension: A Novel Approach, Jashanpreet Kaur, Gursimran Kaur, Chamanpreet Kaur, Sandeep Kumar
Journal of Food and Drug Analysis
Pulmonary Hypertension (PH) is a progressive and potentially fatal condition marked by high pulmonary arterypressure, resulting in heart failure and reduced oxygenation. Despite advancements in treatments, therapeutic optionsfor PH remain limited, particularly in cases resistant to conventional therapies. In biomedical research, nanotechnologyhas become a potential area of study, presenting novel approaches to drug delivery and tissue targeting. Nanosponges, aclass of nanoparticles with porous structures, have gained attention for their ability to encapsulate therapeutic agents,enhance drug stability, and provide controlled release. Nanosponges can be engineered to deliver vasodilators, anti-inflammatory drugs, and gene therapies directly to the pulmonary vasculature, minimizing systemic side …
In Vivo And In Vitro Perspectives In Parkinson's Disease: Mechanisms And The Role Of Phytomedicine,
2025
Department of pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Bela, Ropar, Punjab, India, PIN: 140111
In Vivo And In Vitro Perspectives In Parkinson's Disease: Mechanisms And The Role Of Phytomedicine, Jeewanjot Singh, Devinder Kumar
Journal of Food and Drug Analysis
Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by dopaminergic neuronal loss,oxidative stress, neuroinflammation, and a-synuclein aggregation. Despite advances, current treatments only offersymptomatic relief without altering disease progression. This review aims to evaluate the therapeutic potential of plant-derived phytochemicals in PD through evidence from both in vivo and in vitro experimental models. Key findingsindicate that rodent models (e.g., MPTP, rotenone) and neuronal cell lines (e.g., SH-SY5Y) help replicate PD pathologyand mechanistic insights. Phytochemicals such as baicalein, morin, ferulic acid, and bacopa demonstrate neuroprotectiveeffects via antioxidant, anti-inflammatory, and mitochondrial-stabilizing pathways. Nanotechnology and brain orga-noids further improve translational relevance. In conclusion, …
Global Research Trends On Avocado Oil And Its Fatty Acid Composition: A Bibliometric Review,
2025
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Global Research Trends On Avocado Oil And Its Fatty Acid Composition: A Bibliometric Review, Ahmad Kamrullah Aras, Abdul Rohman
Journal of Food and Drug Analysis
The high content of unsaturated fatty acids in avocado oil (Persea americana) has prompted in-depth exploration across research subjects. This study uses bibliometric analysis to assess global research trends on avocado oil and its fatty acid composition from 2004 to 2024. The search string retrieved 255 articles from the Scopus database and then analyzed using R BibliometriX and VOSviewer software. These tools analyze descriptive structures and collaborative patterns and create network data maps. For comparison, we separated the data into two research periods, namely 2004-2013 and 2014-2024. There was a significant increase in the number of publications, with …
Synthesis, Pharmacokinetic Studies, And Metabolite Analysis Of Meridianin C In Rats Using A Validated Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry Method,
2025
Jilin Medical University, Jilin, China.
Synthesis, Pharmacokinetic Studies, And Metabolite Analysis Of Meridianin C In Rats Using A Validated Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry Method, Liangyu Xu, Zhaogen Wu, Karna Ramachandraiah, Guihun Jiang, Guozhe Zhang
School of Graduate Studies Faculty Publications
Introduction: Meridianin C (MC) is a marine-derived indole alkaloid that has demonstrated kinase inhibitory and anti-tumor activities. Despite its diverse biological properties, no previous reports have systematically evaluated the in vivo quantitative analysis of MC and its metabolites. Methods: In this study, MC was synthesized following a previously reported procedure with slight modifications. A sensitive, accurate, and reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) method was developed to simultaneously detect MC and its five major metabolites (MC-1-N-O-GluA, MC-1-N-O-SO3H, MC-2′-N-O-GluA, MC-2′-N-O-SO3H, and MC-O-GluA-didehydration) in rat plasma. Rats received a single oral dose of MC (100 mg/kg), and pharmacokinetic analysis was …
Human Adipose Stem Cell-Derived Small Extracellular Vesicles Modulate Glial Cell Response In Aged And Young Tbi Mouse Models,
2025
University of South Florida
Human Adipose Stem Cell-Derived Small Extracellular Vesicles Modulate Glial Cell Response In Aged And Young Tbi Mouse Models, Salma S. Abdelmaboud
USF Tampa Graduate Theses and Dissertations
Traumatic brain injury (TBI) is a leading cause of long-term neurological impairment, with aging significantly increasing vulnerability and worsening outcomes. Older individuals experience greater cognitive and motor deficits after TBI and often show diminished responses to therapeutic interventions. Both aging and TBI independently promote heightened neuroinflammation and cognitive dysfunction, increasing susceptibility to poor recovery. This study investigated the therapeutic potential of small extracellular vesicles (sEVs) derived from human adipose-derived stem cells (hASCs) to promote neurological recovery and modulate neuroinflammation in a mouse model of TBI. Male C57BL/6 mice aged 3, 15, and 20 months underwent controlled cortical impact (CCI) and …
Enhancing Cataract Surgery Outcomes: Optimal Use Of Pre- And Post-Operative Eye Drops,
2025
Nova Southeastern University
Enhancing Cataract Surgery Outcomes: Optimal Use Of Pre- And Post-Operative Eye Drops, Keith Skolnick M.D., Anu Valiaveedu
Mako: NSU Undergraduate Student Journal
Many preoperative and postoperative cataract patients struggle with comprehending the use of prescription medication as directed. Language barriers and low health literacy levels are major factors contributing to improper use of prescriptions. To increase patients comprehension, the Fort Lauderdale Eye Institute employed an educational intervention consisting of a live presentation and an instructional video. Results found that 44% of patients were hesitant to ask questions to clinical staff, 32% felt overwhelmed, and nearly 70% lacked confidence in using their prescribed eye drops. Following the intervention, 91% of patients reported increased confidence in their medications, and most indicated that the video …
Muscarinic Acetylcholine Receptor 1: Implications For Precision Medicine And Rett Syndrome Therapeutics,
2025
Loyola University of Chicago Graduate School
Muscarinic Acetylcholine Receptor 1: Implications For Precision Medicine And Rett Syndrome Therapeutics, Mackenzie Smith
Dissertations
Rett syndrome (RTT) is a rare neurodevelopmental disorder caused by mutations in the gene coding for methyl-CpG binding protein 2 (MeCP2). Currently, there is no cure for RTT and few effective treatment options to address the social, cognitive, motor, and respiratory symptoms that patients face. With the goal of identifying novel targets, our lab performed RNA-sequencing experiments in human autopsy samples and found decreased levels of the M1 muscarinic acetylcholine receptor. We have established that potentiation of this receptor in a Mecp2+/- mouse model of RTT rescues social, cognitive, and respiratory phenotypes, providing support for using drugs that act on …
Effect Of Histidine Tag On The Enzymatic Activity Of Pseudolysin And Its Interaction With The Streptomyces Metalloprotease Inhibitor (Smpi) Protein,
2025
Stephen F Austin State University
Effect Of Histidine Tag On The Enzymatic Activity Of Pseudolysin And Its Interaction With The Streptomyces Metalloprotease Inhibitor (Smpi) Protein, Adewale Adeboye Adewole
Electronic Theses and Dissertations
Pseudolysin (PLN) protein is a major virulent factor in Pseudomonas aeruginosa infection. PLN belongs to the family of zinc-dependent metalloproteases. It is naturally inhibited by a smaller protein called streptomyces metalloprotease inhibitor (SMPI), secreted by Streptomyces nigrescens. Previous and current studies demonstrate that the interaction between PLN and SMPI offers unique mechanistic insights for drug development against infections caused by P. aeruginosa. This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography …
Paediatric Fixed Drug Eruption: A Case Report Of Ofloxacin As The Culprit,
2025
School of Pharmacy, Sharda University, Greater Noida, Uttar Pradesh, India 201310
Paediatric Fixed Drug Eruption: A Case Report Of Ofloxacin As The Culprit, Ambika Nand Jha, Varsha Ratan Gaikwad, Rabia Alam, Shivanshu Sharma, Ashok Kumar Gupta
Chulalongkorn Medical Journal
Ofloxacin, a second-generation fluoroquinolone, is a relatively rare but documented cause of fixed drug eruption (FDE), a localized cutaneous hypersensitivity reaction marked by recurrent, well-demarcated erythematous patches or macules that may develop into blisters upon re-exposure to the offending agent. The underlying mechanism is immune-mediated, involving T-cell activation, cytokine release, and keratinocyte apoptosis. A 5-year-old boy weighing 15 kg was prescribed Ofloxacin syrup (7.5 ml twice daily) and Paracetamol suspension (5 ml twice daily) for suspected enteric fever. Within two days, he developed several annular erythematous lesions on his hands and back, associated with intense itching, raising suspicion of a …
Impact Of S-Phase Kinase Protien 2 Blockades On Hematopoetic Stem Cell Metabolism,
2025
California State University - San Bernardino
Impact Of S-Phase Kinase Protien 2 Blockades On Hematopoetic Stem Cell Metabolism, Nicole Elmaraghy
Electronic Theses, Projects, and Dissertations
Hematopoietic stem and progenitor cells (HSPCs) quiescence is vital for the success of bone marrow transplantation, as it preserves long term self- renewal and prevents premature exhaustion (Takubo et al., 2013; Wilson et al., 2008). However, bone marrow transplant (BMT) failure remains a clinical challenge, often due to lack of long-term engraftment and insufficient stress reliance. Both of these characteristics are tightly linked to disrupted stem cell quiescence and metabolic imbalance (Anso et al., 2017; Vannini et al., 2016). One key player is S-phase kinase protein (SKP2), an E3 ubiquitin ligase that targets cell cycle inhibitors, like p27, for proteosome …
Yiqi Yangyin Tongluo Prescription Targets Lncrna Vim-As1 To Regulate Foxk2/Mtor To Promote Autophagy And Inhibit Renal Tubular Epithelial Cell Apoptosis,
2025
Department of Traditional Chinese Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China
Yiqi Yangyin Tongluo Prescription Targets Lncrna Vim-As1 To Regulate Foxk2/Mtor To Promote Autophagy And Inhibit Renal Tubular Epithelial Cell Apoptosis, Rucui Yu, Ruiying Wu, Tingting Chen, Jingwei Xu
Journal of Food and Drug Analysis
Diabetic nephropathy (DN) is one dominating reason for death in diabetic patients, and its incidence is high. It has been reported that Yiqi Yangyin Tongluo prescription (YYTP) can relieve inflammation, and it owns better clinical effects in the treatment of DN. However, the molecular mechanisms of YYTP in the treatment of DN still keep unclear, and deeply investigations are needed. In this study, it firstly was manifested that YYTP can repress the lncRNA VIM-antisense 1 (VIM-AS1) expression in high glucose (HG)-evoked HK-2 cells. Overexpression of VIM-AS1 roll-backed the inhibitive impacts of YYTP on cell apoptosis in HG-triggered HK-2 cells. Additionally, …
