Lucigenin-Pyrogallol Chemiluminescence For The Multiple Detection Of Pyrogallol, Cobalt Ion, And Tyrosinase,
2021
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun, Jilin, PR China
Lucigenin-Pyrogallol Chemiluminescence For The Multiple Detection Of Pyrogallol, Cobalt Ion, And Tyrosinase, Islam M. Mostafa, Muhammad Rehan Hasan Shah Gilani, Yequan Chen, Baohua Lou, Jianping Li, Guobao Xu
Journal of Food and Drug Analysis
Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-H2O2 CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity. Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD = 0.94 μM), lucigenin (LOD = 0.42 μM), cobalt (LOD = 68 μM), and tyrosinase (LOD = 1.8 μg/mL). Furthermore, …
Determination Of Cannabinoids In Hemp Oil Based Cosmetic Products By Lc-Tandem Ms,
2021
Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan
Determination Of Cannabinoids In Hemp Oil Based Cosmetic Products By Lc-Tandem Ms, Yun-Hua Hsu, Ming-Chih Fang, Shou-Chieh Huang, Ya-Min Kao, Su-Hsiang Tseng, Der-Yuan Wang
Journal of Food and Drug Analysis
Cosmetic products containing hemp seed oil as permitted raw materials required the specific compound delta-9-tetrahydrocannabinol (THC) below 10 µg/g. THC was the main psychoactive constituent of cannabis. Since hemp seed oil became an increasingly popular ingredient in cosmetics over the last few years, an efficient and reliable analytical method for THC and other cannabinoids in cannabis-infused cosmetic products was in need. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of delta-9-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN) in hemp seed oil based cosmetic products was developed. Method validation was performed by fertilizing blank samples with analytes and internal …
Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms,
2021
Shaanxi University of Science and Technology, China
Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms, Wei Jia, Yuyang Liu, Xi Xu, Yanxi Zhang, Lin Shi
Journal of Food and Drug Analysis
A novel magnetic adsorbent solid-phase Fe3O4@ZIF-8 extraction method coupled with UHPLC Q-Orbitrap high resolution mass spectrometry for the sensitive and high efficiency detection of fluoroquinolones, tetracyclines, dyes and amphenicols in eggs is described. Under optimum conditions, CCβ and CCα of 0.62 to 26.97 μg.kg–1 and 0.36 to 16.37 μg.kg–1 was obtained, which is much better than that obtained by conventional methods. The accuracy, expressed as recovery, were achieved falling between 75% and 104%. The proposed strategy provides several advantages of simplicity and low energy consumption, thus retaining potential for the screening of …
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication,
2021
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication, Chia-Hsin Lee, Hui-Chi Huang, Mei-Chun Tseng, Chao-Jung Chen
Journal of Food and Drug Analysis
Herein, we describe a rapid, sensitive, and nondestructive method—extractive nanoelectrospray ionization-mass spectrometry (EnESI-MS)—for traditional Chinese medicine (TCM) authentication. The mass-spectral fingerprints of volatile compounds released from various TCMs can be rapidly acquired using EnESI-MS without sample pretreatment. EnESI-MS was applied to successfully differentiate between two commonly used medicinal herbs, Schisandra Chinensis and Schisandra sphenanthera, which are morphologically similar but exhibit different therapeutic effects. Specific volatile compounds of each herb in a ten-component Chinese herbal product, Jia Wei Xiao Yao San, were also identified, and the method was applied to discriminate between the commercial product and a substandard version.
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells,
2021
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells, Hung-Tse Huang, Chia-Ching Liaw, Chun-Tang Chiou, Kung-Ta Lee, Yao-Haur Kuo
Journal of Food and Drug Analysis
Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (1H-1H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPa in 3T3-L1 …
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells,
2021
Department of Urology, E-Da Cancer Hospital, Kaohsiung, Taiwan
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells, Yu-Chi Chen, Chiao-Ling Hsieh, Bu-Miin Huang, Yungchia Chen
Journal of Food and Drug Analysis
Natural products have long been considered as a kind of complementary medicine. In this study, we investigate the apoptotic effect of essential oils of Toona sinensis roots (TSR) on human clear cell renal cell carcinomas (ccRCC). The sesquiterpene content of TSR essential oil was determined via GC/MS analysis. TSR decreased ccRCC cell viabilities, inducing ROS generation and reduction of the mitochondrial membrane potential. Moreover, TSR inhibited Bcl-2 and Hsp90 expression but increased PARP-1 cleavage and cytochrome c release. Akt, mTOR and NF-kB phosphorylation and HIF-a expression were all inhibited, which likely contributed to the anti-proliferative and anti-adhesive effects of TSR.
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition,
2021
Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition, Che-Lun Hsu, Mingchih Fang, Hui-Chun Tseng, Ya-Tze Lin, Su-Hsiang Tseng, Der-Yuan Wang, Deng-Fwu Hwang
Journal of Food and Drug Analysis
The compliance assessment on the labeling of food additives is a hard job, because there are nearly thousand legal food additives can be used in food, and countless illegal additives must also deal with. This study developed a nontargeted data acquisition screening method based on liquid chromatography high resolution mass spectrometry (HRMS) in which a precursor ion and two product ions of each analyte are able to be recorded. The high throughput screening method worked as foodomics that characterized and identified every food components as long as they were ionized in terms of theory. The data acquisition method called data …
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment,
2021
Department of Safety, Health and Environmental Engineering, Ming Chi University of Technology, New Taipei City, Taiwan
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment, Zhen-Shu Liu, Chuen-Fu Lin, Po-Wen Chen
Journal of Food and Drug Analysis
Lactoferrin (LF) is secreted by mammals and displays extensive biological effects. We previously reported that bovine LF (BLF) can boost the cold tolerance of a well-applied probiotic strain, Lactobacillus rhamnosus GG (LGG), to grow robustly under a cold environment, but the molecular mechanism is not clear. Here, RNA-seq analysis was conducted to ascertain molecular pathways underlying cold tolerance exerted by BLF. LGG was cultured in a cold environment (22°C) in the presence or absence of BLF. Transcriptome analysis indicated that BLF significantly elicited 1.2 to 3.2 fold (log2 Fold change) higher expression levels of genes related to stress, defense, cell …
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine,
2021
Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine, Hui-Ling Lee, Chih-Yuan Kang, Yen-Jung Kuo, Shan-Ni Tseng
Journal of Food and Drug Analysis
Magnetic solid phase extraction (MSPE) have been widely applied in a variety of sample preparation techniques. Herein, Fe3O4@pDA as the sorbents for MSPE, were developed for the determination of phenolic acids and flavonoids in fruit wine samples in combination with LC-MS/MS. The Fe3O4@pDA were characterized by Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), Superconducting Quantum Interference Device Magnetometer (SQUID) and thermogravimetric analysis (TGA) in detail. In the present study, a new, rapid, and efficient MSPE by LC-MS/MS was established for the extraction and sensitive detection of phenolic acids and flavonoids. Under the optimized condition …
Spasmolytic Effect Of Grewia Asiatica Fruit Extract On Isolated Smooth Muscles Is Mediated Via Multiple Pathways,
2021
Aga Khan University
Spasmolytic Effect Of Grewia Asiatica Fruit Extract On Isolated Smooth Muscles Is Mediated Via Multiple Pathways, Muhammad Nabeel Ghayur, Saeed Ahmad, Anwarul Hassan Gilani
Department of Biological & Biomedical Sciences
Background: Grewia asiatica Linn, or phalsa, is a commonly consumed fruit in Pakistan. The fruit is employed in the traditional medicine practice of Pakistan as a smooth muscle relaxant in different gastrointestinal (GI) and cardiovascular diseases. In this investigation, we show the antispasmodic and vasorelaxant actions of Grewia asiatica fruit extract.
Methods: A 70% methanolic crude extract of the plant material was prepared (Ga.Cr). Different isolated GI tissue preparations and endothelium-intact aortas from rats were utilized to observe the pharmacological actions of the extract.
Results: Ga.Cr, in increasing concentrations, inhibited the spontaneously contracting rabbit jejunum. In an effort to determine …
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand,
2021
Prince of Songkla University
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand, Ampapan Naknaen, Waraporn Ratsameepakai, Oramas Suttinun, Yaowapa Sukpondma, Eakalak Khan, Rattanaruji Pomwised
Civil and Environmental Engineering and Construction Faculty Research
The Songkhla Lake Basin (SLB) located in Southern Thailand, has been increasingly polluted by urban and industrial wastewater, while the lake water has been intensively used. Here, we aimed to investigate cyanobacteria and cyanotoxins in the SLB. Ten cyanobacteria isolates were identified as Microcystis genus based on16S rDNA analysis. All isolates harbored microcystin genes, while five of them carried saxitoxin genes. On day 15 of culturing, the specific growth rate and Chl-a content were 0.2–0.3 per day and 4 µg/mL. The total extracellular polymeric substances (EPS) content was 0.37–0.49 µg/mL. The concentration of soluble EPS (sEPS) was 2 times higher …
Comparative Analysis Of N95 Respirators Fit Testing With Commercially Available And In House Reagent,
2021
Aga Khan University
Comparative Analysis Of N95 Respirators Fit Testing With Commercially Available And In House Reagent, Erum Khan, Joveria Farooqi, Humaira Shafaq, Kaleem Ullah Khushik, Syed Shamim Raza, Sara Khan, Zohra Rafiq, Bushra Ahmed, Shazia Chisti, Kehkashan Imtiaz, Hasnain Zafar, Afia Zafar
Department of Pathology and Laboratory Medicine
Background: Due to COVID-19, thousands of healthcare workers have been affected and have lost their lives in the line of duty. For the protection of healthcare workers, WHO and CDC have made standard guidelines and requirements for PPE use. N95 masks are amongst the most readily used PPE by healthcare professionals and it is highly recommended by OSHA that every make and model of N95 should go through a fit test at least once in a year.
Method: A total of 30 randomly selected healthcare professionals (who were a regular user of N95 respiratory masks) were subjected to assess in-house …
Anti-Angiogenic Effects Of Chemotherapeutic Agents And Their Enhancement With Ot-515 And S-Nach,
2021
The American University in Cairo AUC
Anti-Angiogenic Effects Of Chemotherapeutic Agents And Their Enhancement With Ot-515 And S-Nach, Shimaa Adel Heikal
Theses and Dissertations
Chemotherapeutic agents have been used for the treatment of numerous types of tumors with great success. Cisplatin and Doxorubicin are among the well-known chemotherapeutic drugs that showed efficacy against various types of cancers. However, cell resistance and major side effects like chemotherapy-induced peripheral neuropathy (CIPN) are limiting factors in using these compounds. Using a combination or adjuvant compounds with anti-angiogenic effects is one of the strategies suggested to decrease resistance or ameliorate chemotherapeutic toxicity. The present study investigated the anti-angiogenic effects of Cisplatin and Doxorubicin alone and combined with Sulfated non-anticoagulant heparin (S-NACH), a low molecular weight heparin LMWH that …
The Link Between Diabetes Mellitus And Tau Hyperphosphorylation: Implications For Risk Of Alzheimer's Disease,
2021
Nova Southeastern University
The Link Between Diabetes Mellitus And Tau Hyperphosphorylation: Implications For Risk Of Alzheimer's Disease, Amy L. Hobday, Mayur S. Parmar
HPD Articles
Diabetes mellitus (DM) is characterized by hyperglycemia caused by a lack of insulin, insulin resistance, or both. It is associated with the development of secondary complications resulting in several comorbidities. Recent studies have revealed an increased risk of developing cognitive dysfunction or dementia in diabetes patients. Diabetes mellitus is considered a risk factor for many neurodegenerative diseases, including Alzheimer's disease (AD). There is increasing evidence to support a link between DM and AD. Studies have shown the dysfunction of insulin signaling in the brain, resulting in increased tau protein phosphorylation (hyperphosphorylation), a hallmark and biomarker of AD pathology, leading to …
Standardizing Data Reporting In The Research Community To Enhance The Utility Of Open Data For Sars-Cov-2 Wastewater Surveillance.,
2021
University of Notre Dame
Standardizing Data Reporting In The Research Community To Enhance The Utility Of Open Data For Sars-Cov-2 Wastewater Surveillance., Jill S. Mcclary-Gutierrez, Zachary T. Aanderud, Mitham Al-Faliti, Claire Duvallet, Raul Gonzalez, Joe Guzman, Rochelle H. Holm, Michael A. Jahne, Rose S. Kantor, Panagis Katsivelis, Katrin Gaardbo Kuhn, Laura M. Langan, Cresten Mansfeldt, Sandra L. Mclellan, Lorelay M. Mendoza Grijalva, Kevin S. Murnane, Colleen C. Naughton, Aaron I. Packman, Sotirios Paraskevopoulos, Tyler S. Radniecki, Fernando A. Roman, Abhilasha Shrestha, Lauren B. Stadler, Joshua A. Steele, Brian M. Swalla, Peter Vikesland, Brian Wartell, Carol J. Wilusz, Judith Chui Ching Wong, Alexandria B. Boehm, Rolf U. Halden, Kyle Bibby, Jeseth Delgado Vela
Faculty and Staff Scholarship
SARS-CoV-2 RNA detection in wastewater is being rapidly developed and adopted as a public health monitoring tool worldwide. With wastewater surveillance programs being implemented across many different scales and by many different stakeholders, it is critical that data collected and shared are accompanied by an appropriate minimal amount of meta-information to enable meaningful interpretation and use of this new information source and intercomparison across datasets. While some databases are being developed for specific surveillance programs locally, regionally, nationally, and internationally, common globally-adopted data standards have not yet been established within the research community. Establishing such standards will require national and …
Impact Of Sodium Dichloroacetate Alone And In Combination Therapies On Lung Tumor Growth And Metastasis,
2021
United Arab Emirates University
Impact Of Sodium Dichloroacetate Alone And In Combination Therapies On Lung Tumor Growth And Metastasis, Aya Mudhafar A. Al-Azawi
Theses
Lung cancer is the second most common form of cancer with the highest mortality rate worldwide in 2020 despite the advances in targeted- and immuno-therapies. Metabolic reprogramming has been recognized as an essential emerging cancer hallmark in which altered metabolic pathways represent an attractive therapeutic target. Sodium Dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, effect has been investigated in various tumors. Building on the already published data, this pre-clinical study aims to explore the anticancer potential of DCA in lung cancer alone and in combination with chemo- and targeted therapies using two non-small cell lung cancer (NSCLC) cell lines …
Analysis Of Transplantable Photoreceptor Replacement Cell Chemotactic Mechanisms In Retinal Biomimetic Ligand Gradients,
2021
CUNY Graduate Center
Analysis Of Transplantable Photoreceptor Replacement Cell Chemotactic Mechanisms In Retinal Biomimetic Ligand Gradients, Onyekwere Onwumere
Dissertations, Theses, and Capstone Projects
Disease or trauma induced loss of rod and cone photoreceptors is a major cause of vision loss worldwide. To replace lost photoreceptors and restore vision, laboratories around the world are investigating photoreceptor replacement strategies using subretinal injection of photoreceptor precursor cells (PPCs) and retinal progenitor cells (RPCs). A major obstacle in the field is that transplanted photoreceptor replacement cells exhibit extremely limited migration into host retina following injection into the subretinal space. In this work, we show that PPCs and host retinal interaction bioinformatics modeling can identify key pathways involved in photoreceptor replacement cell migration and invasion behaviors. In addition, …
Connecting Communities To Coastal Resilience: Enhancing Sustainability Through Public Participation In Salt Marsh Management And Restoration In Suffolk County, Ny,
2021
CUNY Graduate Center
Connecting Communities To Coastal Resilience: Enhancing Sustainability Through Public Participation In Salt Marsh Management And Restoration In Suffolk County, Ny, Jennifer L. Mcgivern
Dissertations, Theses, and Capstone Projects
Coastal resiliency is becoming significantly more critical to the livelihood of coastal communities as the frequency and intensity of storm events increases and is exacerbated by rising sea levels due to climate change. In October 2012 Superstorm Sandy impacted the New York-New Jersey area costing over $70 billion in storm damages and 147 lives lost, as storm surges surpassed record highs for the region. Protruding more than 100 miles into the Atlantic Ocean with over 1,000 miles of shoreline, Long Island is particularly vulnerable to the increasingly ferocious and numerous storms as well as the rising sea levels that climate …
Do Environmental Toxins Predict Violent Crimes?,
2021
Kennesaw State University
Do Environmental Toxins Predict Violent Crimes?, Tyler Stahl
Symposium of Student Scholars
Do chemical pollutants that persistent in the environment and bioaccumulate in the body affect human health and behavior? Could these Persistent, Bioaccumulative, and Toxic (PBT) chemicals play a role in the cause of violent crimes due to deterioration of mental and cognitive functions? In the past, Mercury, a PBT chemical, has been shown in salmon to be associated with aggression. Could similar aggression occur in humans exposed to mercury through a toxic spill? Two sources of data are utilized in this analysis. The Environmental Protection Agency’s (EPA) Annual Toxic Release Inventory publishes data on toxic releases into the environment and …
Exploring Fused Deposition Modeling (Fdm) Three-Dimensional Printing Tablet Design Options For Pharmaceutical Dosage Forms,
2021
New Jersey Institute of Technology
Exploring Fused Deposition Modeling (Fdm) Three-Dimensional Printing Tablet Design Options For Pharmaceutical Dosage Forms, Guluzar Gorkem Buyukgoz
Dissertations
This dissertation examines the use of Fused Deposition Modeling (FDM) based three-dimensional (3D) printing approach for developing patient-specific dosage forms and addressing related technical challenges in such drug delivery systems. The first main objective is to explore pharmaceutical tablet design options using novel FDM 3D printing technology as the drug delivery platform such that drug form and tablet properties are tailored by considering patient age-specific formulations and dissolution control. Of the five different design options, two proposed options meet the main objective of providing similar drug release, whereas the popular option of fixed drug concentration but differing tablet size could …
