Pharmacology, Toxicology and Environmental Health Commons^™

Development Of A Chemical Biology Approach To Uncover The Influence Of Sequence Variations On Ces1 Activity In Live Cells, Samuel James Knebel

Masters Theses

Drug metabolism is the biochemical process of modifying drugs to detoxify and remove them through enzymatic transformations. These biotransformation’s occur primarily in the liver and are critical to understanding how pharmaceutical compounds are chemically altered inside the human body. Human carboxylesterases (CESs) catalyze the hydrolysis of esters, amides, thioesters, and carbamates. CES-mediated hydrolysis plays an important role in the metabolism of many drugs including the first FDA approved antiviral treatment for COVID-19, remdesivir (Veklury), the seizure control medication rufinamide (Banzel), and the flu antiviral drug oseltamivir (Tamiflu). CES activity is known to be influenced by a variety of factors including …

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports (ETD)

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

Characterization Of Quality Attribute And Specification For Standardization Of Acemannan Extracted From Aloe Vera For Tissue Regeneration, Siriwan Seeleamngam, Polkit Sangvanich, Pasutha Thunyakitpisal, Vorasit Vongsutilers

The Thai Journal of Pharmaceutical Sciences

Acemannan extracted from Aloe vera was well characterized as an acetylated mannose polysaccharide which have promising clinical profile for tissue regeneration. Quality aspect of acemannan is required to develop the herbal extract for practical industrial application and reliable therapeutic use. The research aim is to investigate the qualify attributes of acemannan that have impact on its safety and efficacy. Quality of three production batches of acemannan were evaluated based on ICH quality guidelines and pharmacopeial standard. The quality aspect of acemannan were determined by analytical technique including FT-IR, SEC-HPLC, RP-HPLC, GC, and loss on drying to ensure its identify and …

Docking, Synthesis Of Some Novel Flavonoid Analogues, And Their Biological Evaluation, Ishwar Bhat K, Afiya Abdul Aziz, Abhishek Kumar, Vijaya Bhaskar K, Vijay Kumar M

The Thai Journal of Pharmaceutical Sciences

Cancer remains a major class of morbidity and mortality despite basic and clinical research and trials of promising new therapies. In the current era, many drugs are marketed with flavonoid nucleus. Based on this information, this study aims to synthesize and screen flavones for their anticancer activity. Docking studies for cytotoxic activity of flavonoid derivatives targeting tankyrase enzyme were performed. A new series of flavonoid derivatives were prepared from chalcones. Chalcones were prepared between substituted benzaldehyde and 2-hydroxy acetophenone in the presence of potassium hydroxide as catalyst. The flavone derivatives were characterized based on IR, 1H NMR, and Mass spectral …

Solubility Enhancement Of Diacerein By Spray Drying Technique, Formulation Development, And In Vivo Study, Devidas G. Bachhav, Somashekhar S. Khadabadi, Leena P. Deore, Vilas B. Ghavate

The Thai Journal of Pharmaceutical Sciences

Purpose: The aim of the present research was solubility and dissolution rate enhancement of diacerein by spray drying technique. Materials and Methods: Solid dispersions of diacerein with PEG-600, guar gum, poloxamer-188 and aerosil were prepared in different ratios using 32 factorial designs. FTIR, DSC, XRD and SEM were performed to study the interaction between drug and polymers and evaluated for in-vitro drug release study. Results and Discussion:In spray dried solid dispersion formulations there was decrease in crystallinity of diacerein, which leads to increase in dissolution of diacerein from solid dispersions. Diacerein 50 mg immediate release tablets formulated (using spray dried …

Effect Of Spinach Enriched With Zinc And Ω-3/Vitamin E Treatment Against Fluoride-Induced Nephrotoxicity And Bone Calcification Disturbance In Rats, Anfal Djouadi, Samir Derouiche

The Thai Journal of Pharmaceutical Sciences

Objective: The goal of this study was to evaluate the alone or associated spinach, zinc, and ω-3/Vitamin E capacity to improve the renal function and bone calcification altered by fluoride in Wistar rats. Methods: In this study, 36 female Wistar albino rats were used; divided into six groups (n = 6); all groups except the control rats received orally 400 ppm of fluoride as sodium fluoride (NaF) in drinking water during 70 days and then four groups treated in the past 15 days by: Spinach powder, zinc, ω-3/Vitamin E, and spinach+ zinc + ω-3/Vitamin E. Some biochemical and histological markers …

The Efficacy Of Vitamin B6 And Alpha-Lipoic Acid In Preventing Levetiracetam Depressant-Like Behavior In Mice, Azadeh Mesripour, Nasime S. Musavie

The Thai Journal of Pharmaceutical Sciences

Background: Some antiepileptic drugs for instance levetiracetam cause depression in patients. Vitamin B6 is a cofactor involved in the neurotransmitter synthesis. α-Lipoic acid (ALA) is a mitochondrial cofactor that can prevent neuronal damage. The aim of this study was to evaluate the effectiveness of B6 and ALA for preventing levetiracetam depression in mice. Methods: Male NMRI mice (weighing 25 ± 3 g) were used. Levetiracetam (20 mg/kg), and pretreatments with ALA (20, 40 mg/kg), Vitamin B6 (100 mg/kg), or imipramine (10 mg/kg) as the control positive were all administered intraperitonealy for 14 consecutive days. The locomotor test and forced swimming …

Application Of Concept Mapping In Telepharmacy Model Development For Prescription Filling At Primary Care Clusters, Nusaraporn Kessomboon, Siwapond Prasertsuk, Jirachaya Suttima, Nur-Ainee Korsem

The Thai Journal of Pharmaceutical Sciences

Background: Although the Pharmacy Council of Thailand has announced a standard telepharmacy service procedure, there may be some details or limitations that are specific to the primary care cluster (PCC) in the real situation. Objective: This research aimed to create a telepharmacy service model for the PCC using concept mapping techniques. Methodology: Group discussions were held, each participant will submit their opinions and graded of importance and possibilities of each opinion. After that, the data obtained were statistically analyzed to create a concept mapping. Results: From the group discussion, (n = 10) can be concluded that the optimum telepharmacy services …

Barriers And Facilitators Of Wound Care Services Provided By Community Pharmacists In Selangor, Malaysia: An Exploratory Qualitative Study, Hamidi Drahman, Mohd Shahezwan Abd Wahab, Mohd Faiz Mustaffa, Azyyati Mohd Suhaimi

The Thai Journal of Pharmaceutical Sciences

Introduction: Community pharmacists (CPs) are often the first point of contact for patients to obtain health-related advice, including on wound management. Wound care services have been proposed as a community pharmacy practice area to be promoted in Malaysia. Objectives: This study aims to explore the types of wound care services provided by Malaysian CPs, and the factors associated with its provision. Methods: This study utilized the qualitative method to collect in-depth information about the barriers and facilitators associated with the provision of wound care services among a sample of CPs. Interview transcripts were analyzed using thematic content analysis. Results: Twenty-three …

Promoting Healthier Treatment Outcomes In Obese Cancer Patients Taking High Dose Methotrexate, Carly Dell'ova

Walden Dissertations and Doctoral Studies

Cancer and obesity rates continue to rise, creating enormous public heath burdens to the individual and at the national and global levels, reducing quality of life, and increasing spending. Moreover, the relationships between cancer and obesity are not well understood. A commonly used chemotherapy for several types of cancer is high dose methotrexate (HDMTX). Along with chemotherapy, especially at high doses, comes toxicity to specific organs and the entire body. However, limited research has been conducted on obese cancer patients as they are often excluded from clinical studies. Practitioners need to better understand how to dose these patients to provide …

Teaching Agroecology: Preparing Students For Navigating Uncharted Territory, Charles A. Francis, Steve Gliessman

Department of Agronomy and Horticulture: Faculty Publications

Agroecologists understand that farming and food systems are more complex than the aggregation of their components. This realization drives our choices of learning strategies and activities that will prepare students for complexity and uncertainty. Our quest for a just, sustainable, and nutritious food system adequate to equitably serve everyone on the planet today and into the future is an enormous challenge. An undertaking of this magnitude will be met only with major adjustments informed by thoughtful teaching and practicing problem solving skills through a new educational lens. The principles of agroecology help us focus this lens on the wicked problems …

Antibacterial, Antioxidant, And Repellency Potential Of The Essential Oil From Spartium Junceum L. Grown In Lebanon, Hawraa Zahrddin, Mahmoud Khalil, Akram Hijazi

BAU Journal - Science and Technology

The goal of our present study is to assess the pharmacological potential of essential oil extracted from Spartium junceum cultivated in Lebanon. This oil was obtained by hydrodistillation using the Clevenger apparatus. Then, an organoleptic characterization and an evaluation of its antioxidant, repellent/insecticidal, and antibacterial activities have been evaluated. The obtained results showed that this oil had an antioxidant activity reaching 82 % dependent on the dilution used. In addition, it showed an insecticidal effect and an antibacterial effect.

Risk Factors For Emergency Medical Care Or Hospitalization Due To Heat-Related Illness Or Injury: A Systematic Review, Blair Litwiller, Amber N. Barnes

Florida Public Health Review

Background: Temperatures globally have been predicted to increase due to changes in the climate. As the earth gets warmer, it is expected that heat-related illness will also increase worldwide. An effective and appropriate public health response will be critical. Purpose: The aim of this review was to identify risk factors associated with heat-related illness and injury to provide target areas for future interventions. Methods: A review of existing literature was analyzed for risk factors that may increase the likelihood of being admitted to an emergency department for a heat related illness or injury. Results: Of the …

Quantification Of Phosphatidylethanol In Whole Blood And Dried Blood Spots By Lc-Ms/Ms, Elizabeth Yulianto

Student Theses

Phosphatidylethanol (PEth) is an alcohol biomarker that provides a longer window of detection than direct alcohol measurement or other common alcohol metabolites, like ethyl glucuronide. PEth has become useful in assessing drinking history, such as for alcohol-withdrawal treatment, organ transplant settings or prenatal alcohol exposure determination. A method was developed and validated to quantify PEth 16:0/18:1 and 16:0/18:2 in whole blood and dried blood spots (DBS) by liquid chromatography tandem mass spectrometry (LC-MS/MS). The whole blood method was applied to 149 samples from pregnant women, elucidating possible prenatal alcohol exposure. Fifty μL whole blood and 12.7 mm whole punches from …

Inhibition Of Rad18 By Arsenic, Lindsay B. Volk

Biomedical Sciences ETDs

Arsenite exposure leads to the retention of UV-induced DNA damage, thus burdening translesion synthesis (TLS). Rad18 is an essential factor in initiating TLS through PCNA monoubiquitination and is implicated in homologous recombination. It contains two functionally and structurally distinct zinc fingers that are potential targets for arsenite binding. Results from this study reveal arsenite binding to both zinc fingers of Rad18 and a corresponding loss of domain function. Importantly, arsenite inhibited Rad18 RING-dependent PCNA monoubiquitination and polymerase eta recruitment to DNA damage. Further analysis demonstrated multiple effects of arsenite, including the reduction in the nuclear localization and UV-induced chromatin recruitment …

Effectiveness And Safety Of Direct Oral Anticoagulants Among Patients With Non-Valvular Atrial Fibrillation And Multimorbidity, Amol Dhamane, Mauricio Ferri, Allison V. Keshishian, Cristina Russ, Nipun Atreja, Cynthia Gutierrez, Birol Emir, Huseyin Yuce, Manuela Di Fusco

Publications and Research

Introduction

In the USA, there is a steady rise of atrial fibrillation due to the aging population with increased morbidity. This study evaluated the risk of stroke/systemic embolism (S/SE) and major bleeding (MB) among elderly patients with non-valvular atrial fibrillation (NVAF) and multimorbidity prescribed direct oral anticoagulants (DOACs).

Methods

Using the CMS Medicare database, a retrospective observational study of adult patients with NVAF and multimorbidity who initiated apixaban, dabigatran, or rivaroxaban from January 1, 2012 to December 31, 2017 was conducted. High multimorbidity was classified as having ≥ 6 comorbidities. Cox proportional hazard models were used to evaluate the hazard …

Hepatocyte-Hepatic Stellate Cell Axis In Potentiation Of Alcohol And Hiv-Induced Liver Injury, Moses O. New-Aaron

Theses & Dissertations

Human Immunodeficiency Virus (HIV) remains a global threat, with approximately 38.4 million active infections and 33 million HIV-related deaths since the first case. While many may be tempted to think of HIV as a relic of the past, emerging data suggests otherwise. By the end of 2021, approximately 1.5 million HIV incidences and 650 000 mortalities were reported globally. The etiologies of HIV-related mortality are numerous. Liver disease is one of the leading etiologies of HIV-related mortality, especially in recent times of antiretroviral therapy (ART)-induced longevity among people living with HIV (PLWH). While co-infections with hepatotropic viruses notoriously contribute to …

Nanofabrication For Precision Vaccinology, Karen Zagorski

Theses & Dissertations

Vaccines are among the most effective and safe therapeutics known to mankind. Historically the approach to the creation of new vaccines has been dominated by empirical research, trial, and error. This thesis focuses on the more modern, rational approaches to vaccinology based on the current understanding of the inner workings of the immune system. We used nanotechnology for the generation of novel immunogens, addressed several challenges, and characterized the immune responses.

The preparation, characterization, and troubleshooting of several vaccines against dimeric amyloid beta are covered in chapters 4-6. Despite being ultimately unsuccessful, this part provided a deeper understanding of the …

Étude Visant À Mieux Guider L’Évaluation Des Risques Des Travailleurs Exposés Aux Nanoparticules (Np) : Existe-T-Il Différentes Propriétés Inflammatoires Des Np Liées Au Sexe?, Marion Vanharen, Abdelaziz Saafane, Alexanne Léveillé, Thomas Mahbeer, Denis Girard

Rapports de recherche scientifique

Les nanotechnologies offrent un important potentiel d’exploitation dans des secteurs industriels variés, pour lequel les bénéfices financiers ne sont pas négligeables. À l’instar des provinces canadiennes et de nombreux pays industrialisés, le Québec voit un nombre grandissant de compagnies produisant des nanomatériaux et des nanoparticules (NP) se développer, sans oublier celles qui introduisent de plus en plus de NP dans leurs produits. Le nombre de travailleurs québécois œuvrant dans la fabrication et dans la synthèse des NP augmentera dans les années à venir. De plus, le nombre de travailleurs appelés à manipuler et à transformer des NP dans les domaines …

Nanoparticle Conjugated Photosensitizer For Targeted Photodynamic Inactivation Of Cancer Cells, Symone D. Crowder

Honors College Theses

Photodynamic therapy (PDT) is considered to be a potential replacement for traditional methods of chemotherapy. It includes the administration of photosensitizing agents (PS), which generate reactive oxygen species (ROS) upon excitation at a specific wavelength. With new outlooks and techniques, cancer research is advancing each day. It has allowed the progress of several theranostic drug delivery systems (DDS) exploring the area of nanomedicine.2 In the present work, a Rhodamine derivative, Rhodamine 6G (R6G) was used as the PS. In general, rhodamine compounds undergo cytotoxic reactions on photoexcitation by electron transfer reactions with folic acid within cells, making them a favorable …