Pharmacology, Toxicology and Environmental Health Commons^™

Acellular Reactivity Of Polymeric Dendrimer Nanoparticles As An Indicator Of Oxidative Stress In Vitro, Marcus Maher, Humza Khalid, Hugh Byrne

Articles

The need for rapid and cost effective pre-screening protocols of the toxicological response of the vast array of emerging nanoparticle types is apparent and the emerging consensus on the paradigm of oxidative stress by generation of intracellular reactive oxygen species as a primary source of the toxic response suggests the development of acellular assays to screen for nanoparticle surface reactivity. This study explores the potential of the monoamine oxidase A (MAO-A) enzyme based assay with polymeric dendrimers as cofactors and serotonin as substrate, which generates H₂O₂, quantified by the conversion of the Carboxy-H₂DCFDA dye …

Gene Expression Microarray Data From Human Microvascular Endothelial Cells Supplemented With A Low Concentration Of Niacin, Nica M. Borradaile, Jennifer M. Hughes-Large

Physiology and Pharmacology Publications

The systemic lipid modifying drug, niacin, can directly improve human microvascular endothelial cell angiogenic function under lipotoxic conditions, possibly through activation of niacin receptors [1]. Here we provide accompanying data collected using Affymetrix GeneChip microarrays to identify changes in gene expression in human microvascular endothelial cells treated with 10 μM niacin. Statistical analyses of robust multi-array average (RMA) values revealed that only 16 genes exhibited greater than 1.3-fold differential expression. Of these 16, only 5 were identified protein coding genes, while 3 of the remaining 11 genes appeared to be small nuclear/nucleolar RNAs. Altered expression of EFCAB4B, …

Blockade Of Astrocytic Calcineurin/Nfat Signaling Helps To Normalize Hippocampal Synaptic Function And Plasticity In A Rat Model Of Traumatic Brain Injury, Jennifer L. Furman, Pradoldej Sompol, Susan D. Kraner, Melanie M. Pleiss, Esther J. Putman, Jacob Dunkerson, Hafiz Mohmmad Abdul, Kelly N. Roberts, Stephen William Scheff, Christopher M. Norris

Pharmacology and Nutritional Sciences Faculty Publications

Increasing evidence suggests that the calcineurin (CN)-dependent transcription factor NFAT (Nuclear Factor of Activated T cells) mediates deleterious effects of astrocytes in progressive neurodegenerative conditions. However, the impact of astrocytic CN/NFAT signaling on neural function/recovery after acute injury has not been investigated extensively. Using a controlled cortical impact (CCI) procedure in rats, we show that traumatic brain injury is associated with an increase in the activities of NFATs 1 and 4 in the hippocampus at 7 d after injury. NFAT4, but not NFAT1, exhibited extensive labeling in astrocytes and was found throughout the axon/dendrite layers of CA1 and the dentate …

Tom Curran, Phd, Frs, Named Executive Director Of Children's Research Institute, Children's Mercy Hospital

Our Story Continues

Tom Curran, PhD, FRS, has been named Chief Scientific Officer and Executive Director of the Children’s Mercy Children’s Research Institute (CRI), effective Feb. 1 [2016]

The Children’s Research Institute at Children’s Mercy Kansas City was established last year to build on the hospital’s century-long history in pediatric research and to focus the hospital’s future research efforts in four key areas:

• Pediatric Genomic Medicine
• Clinical Pharmacology
• Health Services and Outcomes
• Health Care Delivery

Structural Dependence Of The In Vitro Cytotoxicity, Oxidative Stress And Uptake Mechanisms Of Poly(Propylene Imine) Dendritic Nanoparticles, Humza Khalid, Sourav Prasanna Mukherjee, Luke O'Neill, Hugh Byrne

Articles

The in vitro cytotoxic and intracellular oxidative stress responses to exposure to poly (propylene imine) (PPI) dendritic nanoparticles of increasing generation (number of repeated branching cycles) (G0-G4) were assessed in an immortal non-cancerous human keratinocyte cell-line (HaCaT). Confocal fluorescence microscopy with organelle staining was used to explore the uptake and intracellular trafficking mechanisms. A generation and dose dependent cytotoxic response was observed, increasing according to generation and therefore number of surface amino groups. A comparison of the cytotoxic response of G4 PPI and the related G4 Poly (amido amine) dendrimer indicates that the PPI with the same number of surface …

Role Of Pharmacogenetics In Improving The Safety Of Psychiatric Care By Predicting The Potential Risks Of Mania In Cyp2d6 Poor Metabolizers Diagnosed With Bipolar Disorder., Santiago Sánchez-Iglesias, Virginia García-Solaesa, Belén García-Berrocal, Almudena Sanchez-Martín, Carolina Lorenzo-Romo, Tomás Martín-Pinto, Andrea Gaedigk, José Manuel González-Buitrago, María Isidoro-García

Manuscripts, Articles, Book Chapters and Other Papers

One of the main concerns in psychiatric care is safety related to drug management. Pharmacogenetics provides an important tool to assess causes that may have contributed the adverse events during psychiatric therapy. This study illustrates the potential of pharmacogenetics to identify those patients for which pharmacogenetic-guided therapy could be appropriate. It aimed to investigate CYP2D6 genotype in our psychiatric population to assess the value of introducing pharmacogenetics as a primary improvement for predicting side effects.A broad series of 224 psychiatric patients comprising psychotic disorders, depressive disturbances, bipolar disorders, and anxiety disorders was included. The patients were genotyped with the AmpliChip …

Pharmacogenetic Allele Nomenclature: International Workgroup Recommendations For Test Result Reporting., L V. Kalman, Jag Agúndez, M Lindqvist Appell, J L. Black, G C. Bell, S Boukouvala, C Bruckner, E Bruford, K Caudle, S A. Coulthard, A K. Daly, Al Del Tredici, J T. Den Dunnen, K Drozda, R E. Everts, D Flockhart, R R. Freimuth, Andrea Gaedigk, H Hachad, T Hartshorne, M Ingelman-Sundberg, T E. Klein, V M. Lauschke, D R. Maglott, H L. Mcleod, G A. Mcmillin, U A. Meyer, D J. Müller, D A. Nickerson, W S. Oetting, M Pacanowski, V M. Pratt, M V. Relling, A Roberts, W S. Rubinstein, K Sangkuhl, M Schwab, S A. Scott, S C. Sim, R K. Thirumaran, L H. Toji, R F. Tyndale, Rhn Van Schaik, M Whirl-Carrillo, Ktj Yeo, U M. Zanger

Manuscripts, Articles, Book Chapters and Other Papers

This article provides nomenclature recommendations developed by an international workgroup to increase transparency and standardization of pharmacogenetic (PGx) result reporting. Presently, sequence variants identified by PGx tests are described using different nomenclature systems. In addition, PGx analysis may detect different sets of variants for each gene, which can affect interpretation of results. This practice has caused confusion and may thereby impede the adoption of clinical PGx testing. Standardization is critical to move PGx forward.

Nitrogen And Phosphorus Availability From Various Composted Wastes For Use In Irish Agriculture And Horticulture, Alan Lee

Doctoral

Current environmental EU legislation promotes recycling and recovery from organic waste products. Compost has been identified as an alternative to inorganic fertilisers and animal slurries as a nutrient source for crop plants. This study aimed to investigate nitrogen (N) and phosphorus (P) availability from various composted waste through detailed characterisations, complemented by short term lab incubations and long term plant growth experiments. Twenty-five composts were selected and classified by their groups. The composts were characterised by multiple different analytical techniques. Two incubation studies were conducted. One investigating N and P mineralisation potential of the composts and the second on the …

Dietary Supplementation With Organoselenium Accelerates Recovery Of Bladder Expression, But Does Not Improve Locomotor Function, Following Spinal Cord Injury, Carolyn A. Meyer, Ranjana Singh, Mackenzie T. Jones, Chen-Guang Yu, Ronan F. Power, James W. Geddes

Spinal Cord and Brain Injury Research Center Faculty Publications

Selenium is an essential element required for activity of several antioxidant enzymes, including glutathione peroxidase. Because of the critical role of the antioxidant system in responding to traumatic events, we hypothesized that dietary selenium supplementation would enhance neuroprotection in a rodent model of spinal cord injury. Rats were maintained on either a control or selenium-enriched diet prior to, and following, injury. Dietary selenium supplementation, provided as selenized yeast added to normal rat chow, resulted in a doubling of selenium levels in the spinal cord. Dietary selenium reduced the time required for recovery of bladder function following thoracic spinal cord injury. …

The Real Footprint Of Electric Vehicles, And What That Could Mean For Our Future, Krista Brown

Journal of Environmental and Resource Economics at Colby

This paper looks at the impact of electric vehicles on CO2 emissions specifically based on the type of electricity used in each New England state analyzed. The research looks at the question of whether or not emissions will in fact be lowered as electric cars start to take the majority of vehicles used instead of the conventional cars that take up a much larger percent of the motor vehicles used today. Taking into account the use of electricity and how many sources of electricity also release a substantial amount of emissions, we see how valid the public opinion of electric …

The Effect Of Information On Avoidance Behavior And Bicycle Transportation: A Study Of “Spare The Air” And Bay Area Bikeshare, Paige W. Whistler

Journal of Environmental and Resource Economics at Colby

This paper seeks to address the effect of “Spare the Air” pollution control alerts in the San Francisco Bay Area in California on the use of Bay Area BikeShare as a form of transportation. By observing the daily alerts on the duration of rides using BikeShare, this paper observes if STA ozone alerts are effective in changing human behavior. The results show that only as a subscriber to the BikeShare program and on an alert day does duration decrease whereas customers regardless of if it is an alert day will ride more often and at longer distances. The results contradict …

Socioeconomic Attributes’ Relationship To Green Commercial Office Buildings, Tianyang Zhou

Journal of Environmental and Resource Economics at Colby

This paper examines the relationship between the socioeconomic attributes of a state and its green commercial office space adaptation. I hypothesize that a state with high socioeconomic status will have a high green building adoption. Additionally, the high socioeconomic status states may be more likely to invest in green commercial buildings with higher LEED certification levels, and various types of LEED commericial office projects are affected by the socoeconomic attributes differently.

Do Peers Get Punished: Stock Market Effect Of Bp Oil Spill On Peers, Yuga Koda

Journal of Environmental and Resource Economics at Colby

This paper examines the stock market reaction to the British Petroleum oil spill on April 20, 2010. This event study looks at different sectors that may be affected by the oil spill. It finds that different industries do not have significant abnormal returns, with two exceptions. First, utilities companies have a very small positive abnormal return for a short period of time after the incident. Second, when only looking at BP's direct competitors, there is a statistically significant negative abnormal return, implying that BP's competitors are punished for BP's mishap.

Media Effects On Solar Panel Installations Across 20 States, Casey Romeo

Journal of Environmental and Resource Economics at Colby

This paper looks at the relationship between media coverage of solar power and the solar panel installation at a state level. Specifically, it aims to find if more newspaper articles cause more households to adopt solar energy. Use data from 20 states from 2009 to 2014. I find that there is a modest, yet positive and statistically significant relationship between newspaper articles and installations, which suggests that the media does influence the decision to install solar panels. There is possible endogeneity between these two variables. Disentangling this endogeneity is beyond the scope of this research and would be a topic …

Renewable Energy Consumption: Initiatives In Colorado And Washington, Robin K. Doroff

Journal of Environmental and Resource Economics at Colby

This paper examines the use of policy mechanisms to increase renewable energy consumption within the United States. In order to effectively measure the change of renewable energy consumption, I analyze two initiatives that promote energy consumption from renewable sources and overall increases in energy conservation. Colorado and Washington had approved initiatives in the years 2004 and 2006, respectively. Although the results are not entirely statistically significant, I find that the initiatives lead to increases in total renewable energy consumption and decreases in total energy use.

Stock Market Reactions To The Iarc Announcement Linking Red Meat And Processed Meat To Cancer, Scott B. Lehman

Journal of Environmental and Resource Economics at Colby

This study investigates how food companies were affected by the International Agency for Research on Cancer’s report that linked processed meat and red meat consumption to increased risk of cancer. The results indicate that neither fast food companies nor meat producers were greatly affected by the announcement in the days immediately following the release.

Property Value Changes From Public Transportation: How The Greenbush Commuter Line Affected Property Values, Sarah C. Evans

Journal of Environmental and Resource Economics at Colby

Prior studies have investigated the impact on property values to the proximity of established public transportation stations. Public transportation is thought to increase value of towns and properties, giving residents more options for transportation to and around the city. Both benefits and consequences of public transportation exist, allowing for either a raise or lower of property values in towns with stops. This paper analyzes the Greenbush Commuter line in Massachusetts, seeing if the proximity to the stops has an affect on the property of values in the towns. A difference – in – difference model is used to investigate a …

The Effectiveness Of The Colby College Electricity Competition On Promoting Electricity Conservation To Students, Jeff Vaz

Journal of Environmental and Resource Economics at Colby

This paper examines the effectiveness of the Colby College electricity reduction competition in altering students’ behavior to conserve electricity. The Colby College electricity reduction competition is an information-based policy that Colby implemented to promote the conservation of electricity, which are a major contributor to greenhouse gas (GHG) emissions in the United States. The study analyzes if different residence halls reduce electricity consumption by different amounts during the competition period. Results indicate that only 4 of 22 residence halls reduced electricity usage during competition periods in a statistically significant manner. The study concludes that the electricity competition at Colby College is …

Invited Introduction To Jerec, Matthew Kotchen

Journal of Environmental and Resource Economics at Colby

No abstract provided.

Welcome By The Editor, Nathan W. Chan

Journal of Environmental and Resource Economics at Colby

No abstract provided.