Pharmacology, Toxicology and Environmental Health Commons^™

A Variant Pfcrt Isoform Can Contribute To Plasmodium Falciparum Resistance To The First-Line Partner Drug Piperaquine, Satish K. Dhingra, Devasha Redhi, Jill M. Combrinck, Tomas Yeo, John Okombo, Philipp P. Henrich, Annie N. Cowell, Purva Gupta, Matthew L. Stegman, Jonathan M. Hoke, Roland A. Cooper, Elizabeth Winzeler, Sachel Mok, Timothy J. Egan, David A. Fidock

Biological Sciences Faculty Publications

Current efforts to reduce the global burden of malaria are threatened by the rapid spread throughout Asia of Plasmodium falciparum resistance to artemisinin-based combination therapies, which includes increasing rates of clinical failure with dihydroartemisinin plus piperaquine (PPQ) in Cambodia. Using zinc finger nuclease-based gene editing, we report that addition of the C101F mutation to the chloroquine (CQ) resistance-conferring PfCRT Dd2 isoform common to Asia can confer PPQ resistance to cultured parasites. Resistance was demonstrated as significantly higher PPQ concentrations causing 90% inhibition of parasite growth (IC₉₀) or 50% parasite killing (50% lethal dose [LD₅₀]). This mutation …

Salmonella And Aeromonas Contamination In A 303(D) Listed Water Body Compared To Fecal Indicators & Water Quality Parameters, Elizabeth M. Morgan Ms.

Undergraduate Honors Theses

Since the passage of the Clean Water Act, concern about surface water quality has increased. Reducing exposure to pathogens and adverse impacts on human health because of contact with surface waters has become the focus of many regulatory agencies. Fecal pollution is often a cause of surface water impairment. Fecal indicators, such as fecal coliforms and Escherichia coli, are used as surrogates to evaluate the presence or absence of fecal pollution. However, a growing body of research has shown that these species lack key characteristics necessary to be adequate indicators. As such, explorations into the efficacy of indicator species …

Increasing Marine Debris Awareness Through Public Education And Analyzing Fish For Microplastic And Pollutant Contamination, Stephanie R. Dubois

Theses and Dissertations

Marine debris is any persistent solid material that is manmade and then disposed of into the world’s oceans. Outreach and education was used in combination with biological research using an indicator fish species to raise awareness about the impacts of marine debris and encourage change in behavior to reduce marine debris. Pre and post surveys were administered during marine debris education tours for K-12 students at the Coastal Studies Lab on South Padre Island, Texas to determine change in knowledge and behavior regarding marine debris. Pinfish were collected from Laguna Madre and stomachs and livers removed for microplastics content and …

Determining The Effects Of Social Media Monitoring To Identify Potential Foodborne Illness In Southern Nevada, Lauren Diprete

UNLV Theses, Dissertations, Professional Papers, and Capstones

Foodborne illness, commonly referred to as food poisoning, affects an estimated 1 in 6 Americans every year, despite the fact that it is entirely preventable. Many cases of foodborne illness go unreported; however, better reporting leads to faster health department response and containment. Social media monitoring, using software to identify trends in social media posts, is a novel new tool that has been tested in a variety of public health fields with promising preliminary results. The Southern Nevada Health District (SNHD) has employed social media monitoring software to identify potential foodborne illness within Southern Nevada. The purpose of this study …

Stability Of Synthetic Cathinones In Oral Fluid Samples, Briana Miller, Jiyoung Kim, Marta Concheiro

Publications and Research

Synthetic cathinones are new stimulant drugs derived from cathinone that have been sold as “legal highs” worldwide. These compounds can elicit powerful effects such as delusions, hallucinations as well as other potentially dangerous behavior. New analogs with varying effects and potencies are constantly introduced in the market to evade legislation, and they are not detected by routine screening and confirmation methods. Oral fluid is an alternative matrix of increasing interest in forensic toxicology. Its collection is non-invasive and easily supervised, and positive drug findings typically reflect recent drug exposure. The focus of this research was to develop a method for …

Mercury Concentrations In Snowy Plovers And Sediments Of Their Breeding Environments In The Southern Great Plains, Laura Duffie

Electronic Theses and Dissertations

Western snowy plovers (Charadrius nivosus) are philopatric shorebirds sporadically distributed during the breeding season at saline lake and alkali flat breeding environments of the Southern Great Plains (SGP) of Oklahoma, New Mexico, and Texas. The major conservation threat to interior snowy plovers breeding on the SGP derives from anthropogenic modification of breeding environments. The SGP is one of the most intensively cultivated regions in the world, and excessive groundwater mining and resulting deterioration of artesian spring flows reduces site quantity and quality critical for regional successful nesting. Most saline lakes now primarily receive freshwater inputs via irregular precipitation …

Chronic Toxicity Of Binary‐Metal Mixtures Of Cadmium And Zinc To Daphnia Magna, Edgar Pérez, Tham C. Hoang

School of Environmental Sustainability: Faculty Publications and Other Works

The present study characterized the chronic effect of binary‐metal mixtures of cadmium (Cd) and zinc (Zn) on Daphnia magna. The titration design was chosen to characterize the 21‐d chronic effects of the binary‐metal mixtures on survival, growth, reproduction, and metal accumulation in D. magna. Using this design, increasing concentrations of Zn (10, 20, 40, 80, 120, 160, and 200 μg/L) were titrated against a constant concentration of 1.5 μg/L Cd. The results demonstrated that Cd was highly toxic to D. magna. In a mixture with Cd and Zn, sublethal concentrations of 10 and 20 μg/L Zn were …

Asthma-Related Hospitalization And Emergency Room Visits In Oakland, Alondra Varela

McNair Research Journal SJSU

The City of Oakland has seen diminished childhood asthma rates. During the last few years more children have been able to qualify for Medi-Cal and other health insurance plans under the Affordable Care Act (ACA). Local programs work together with Medi-Cal and ACA to reduce the number of symptoms related to asthma by making sure children from low-income communities have access to primary care, medicine, and treatment. Unfortunately, the asthma-related hospitalizations and emergency room visits among children have continued to increase. These rates are even higher in low-income communities located near highly trafficked areas. To investigate this phenomenon, this study …

Hiv-1 Protease Inhibitors From Marine Brown Alga: A Literature Review, Eric Nunez Aguilar

McNair Research Journal SJSU

The human immunodeficiency virus type 1 (HIV-1) causes an infectious disease that if left untreated can progress to acquired immunodeficiency syndrome (AIDS) and be fatal. Finding a cure and more treatments for HIV has become a top priority in medical research, and due to the cost of synthetic HIV medication, finding a low-cost alternative is essential. Marine pharmacology has provided a possible solution to costly HIV medication through compounds derived from marine brown algae that inhibit the HIV-1 protease (PR). The objective of this study is to emphasize the necessity for further research in HIV-1 protease inhibition using marine wildlife-derived …

Using Freshwater Mussels As An Indicator For River Water Quality, Joseph Gerland, Will Titone, Ariana Clark, Brad Michael

Undergraduate Research Conference

Using the freshwater mussel to monitor water quality is a practical and advantageous way to use nature’s indicator species. River water quality is an important measurement that is constantly monitored for many purposes. The sampling involved with monitoring can be very costly and time-consuming. Using mussels as indicator species could save money and time. So, this project has been dedicated to exploring the applications and reality of using freshwater mussels to monitor river quality.

Physiological Effects Of Five Different Marine Natural Organic Matters (Noms) And Three Different Metals (Cu, Pb, Zn) On Early Life Stages Of The Blue Mussel (Mytilus Galloprovincialis), Lygia Sega Nogueira, Adalto Bianchini, Scott Smith, Marianna Basso Jorge, Rachael L. Diamond, Chris M. Wood

Chemistry Faculty Publications

Metals are present in aquatic environments as a result of natural and anthropogenic inputs, and may induce toxicity to organisms. One of the main factors that influence this toxicity in fresh water is natural organic matter (NOM) but all NOMs are not the same in this regard. In sea water, possible protection by marine NOMs is not well understood. Thus, our study isolated marine NOMs by solid-phase extraction from five different sites and characterized them by excitation-emission fluorescence analysis—one inshore (terrigenous origin), two offshore (autochthonous origin), and two intermediate in composition (indicative of a mixed origin). The physiological effects of …

Constitutive Activation Of Kappa Opioid Receptors At Ventral Tegmental Area Inhibitory Synapses Following Acute Stress., Abigail M Polter, Kelsey Barcomb, Rudy W Chen, Paige M Dingess, Nicholas M Graziane, Travis E Brown, Julie A Kauer

Pharmacology and Physiology Faculty Publications

Stressful experiences potently activate kappa opioid receptors (κORs). κORs in the ventral tegmental area regulate multiple aspects of dopaminergic and non-dopaminergic cell function. Here we show that at GABAergic synapses on rat VTA dopamine neurons, a single exposure to a brief cold-water swim stress induces prolonged activation of κORs. This is mediated by activation of the receptor during the stressor followed by a persistent, ligand-independent constitutive activation of the κOR itself. This lasting change in function is not seen at κORs at neighboring excitatory synapses, suggesting distinct time courses and mechanisms of regulation of different subsets of κORs. We also …

Hawkes Co. V. United States Army Corps Of Engineers, Sarah M. Danno

Public Land & Resources Law Review

A peat mining company will not be required to obtain a permit under the Clean Water Act to discharge dredged and fill material into wetlands. The United States District Court for the District of Minnesota held that the United States Army Corps of Engineers fell short in its attempts to establish jurisdiction over the wetlands by twice failing to show a significant nexus existed between the wetlands and navigable waters. Further, the district court enjoined the Corps from asserting jurisdiction a third time because it would force the mining company through a “never ending loop” of administrative law.

Pharmacology, Pharmacokinetics, And Metabolism Of The Dna-Decoy Ayx1 For The Prevention Of Acute And Chronic Post-Surgical Pain, Julien Mamet, Scott Harris, Michael Klukinov, David C. Yeomans, Renee R. Donahue, Bradley K. Taylor, Kelly Eddinger, Tony Yaksh, Donald C. Manning

Physiology Faculty Publications

Background: AYX1 is an unmodified DNA-decoy designed to reduce acute post-surgical pain and its chronification with a single intrathecal dose at the time of surgery. AYX1 inhibits the transcription factor early growth response protein 1, which is transiently induced at the time of injury and triggers gene regulation in the dorsal root ganglia and spinal cord that leads to long-term sensitization and pain. This work characterizes the AYX1 dose-response profile in rats and the link to AYX1 pharmacokinetics and metabolism in the cerebrospinal fluid, dorsal root ganglia, and spinal cord.

Results: The effects of ascending dose-levels of AYX1 on mechanical …

Therapeutic Modulation Of Cancer Metabolism With Dichloroacetate And Metformin, Nathan Patrick Ward

USF Tampa Graduate Theses and Dissertations

The robust glycolytic metabolism of glioblastoma multiforme (GBM) has proven them susceptible to increases in oxidative metabolism induced by the pyruvate mimetic dichloroacetate (DCA). Recent reports demonstrate that the anti-diabetic drug metformin enhances the damaging oxidative stress associated with DCA treatment in cancer cells. We sought to elucidate the role of metformin’s reported activity as a mitochondrial complex I inhibitor in the enhancement of DCA cytotoxicity in the VM-M3 model of GBM. We demonstrated that metformin potentiated DCA-induced superoxide production and that this was required for enhanced cytotoxicity towards VM-M3 cells with the combination. Similarly, rotenone enhanced oxidative stress resultant …

The Optimization Of The Nuclear Protein In Testis (Nut) Antibody And Its Importance And Impact In The Pathology Lab, Lindsey Martinez

USF Tampa Graduate Theses and Dissertations

Optimization in immunohistochemistry is often a time consuming and complex process. There are a varying array of moving parts to consider all while preserving the sensitivity and specificity of the test. When optimizing an antibody it is important to consider the fixation of the tissue and the type of epitope retrieval that would be best suited for the test. The dilution of the primary antibody is a key marker for the efficiency and effectiveness of the laboratory protocol. The purpose of this study was to produce an optimized antibody for the nuclear protein in testis to detect NUT midline carcinoma …

Trap-Neuter-Return Programs And The Importance Of Associated Public Awareness, Nicolette Sliwa

Biology: Student Scholarship & Creative Works

The focus of this paper is to describe a typical Trap-Neuter-Return (TNR) program and highlight the major pros and cons surrounding this somewhat controversial method of managing the overwhelming free-roaming cat population. The effects of the species’ overabundance on the environment and human health are also discussed. Citing studies completed on TNR programs around the globe as well as mathematical models, the effectiveness of this method is compared to other, currently used alternatives. Finally, it has been suggested that the general awareness level a neighborhood possesses about the true goals of TNR programs has a potentially significant impact on the …

Fish Community Assessment Of The Bonyic And Teribe Rivers Within The Naso-Teribe Territory Bocas Del Toro, Panama: Possible Implications Of Bonyic Dam., Shaylyn Austin

Independent Study Project (ISP) Collection

In the Changuinola/Teribe watershed of Bocas Del Toro, Panama, changes to the fluvial system due to the recently constructed Bonyic Dam have implications connected to the biodiversity of a UNESCO Biosphere Reserve and the livelihoods of thousands of people of the Naso-Teribe indigenous group. This study investigated the composition of fish communities in 6 study sites in 3 different areas in relation to the Bonyic Dam. A total of 318 individual fish were captured, representing 5 families and 10 species, using the cast net sampling method. The biodiversity was analyzed using Simpson’s Diversity Index, Evenness Index, species richness, and species …

Development Of A Wild Infant Woolly Monkey Social Interactions, Time Allocation And Behavior Of A Wild Lagothrix Lagotricha Poeppigii Infant And Her Mother, Saba Zewdie

Independent Study Project (ISP) Collection

The lowland woolly monkey (Lagothrix lagotricha poeppigii), like all species of woolly monkey, is extremely threatened due to anthropologic activities such as deforestation, petroleum drilling and commercial hunting across their habitat. Due to their low reproductive rate and low reproductive success in captivity, the captive population is decreasing every year as habitat loss increases. In late February of 2017 an infant female was born on the island of Sumak Allpa, a monkey rehabilitation organization and reserve in the western Ecuadorian Amazon. The woolly monkeys are completely independent on the reserve; and as such her birth marks the first …

Utilization And Accessibility Of Securinega Virosa For Medical Use In Morogoro Municipality, Sasha Friedrich

Independent Study Project (ISP) Collection

Approximately 80% of rural communities in Tanzania utilize the services of traditional healers. This is largely because modern medicine is often expensive, inaccessible, or has undesirable side effects. This study investigates use of traditional medicine in Morogoro Urban district where people do have access to hospitals. In particular, this study aims to assess the utilization and accessibility the plant species Securinega virosa for medicinal use by identifying and interviewing the actors involved in the supply chain from harvester to consumer. I conducted semi-structured interviews with eight traditional healers and one herbal medicine shopkeeper. I expected harvesters to express a decline …