Open Access. Powered by Scholars. Published by Universities.®

Molecular, Genetic, and Biochemical Nutrition Commons

Open Access. Powered by Scholars. Published by Universities.®

541 Full-Text Articles 1,512 Authors 150,915 Downloads 57 Institutions

All Articles in Molecular, Genetic, and Biochemical Nutrition

Faceted Search

541 full-text articles. Page 23 of 23.

Targeted Deletion Of Hepatocyte Abca1 Leads To Very Low Density Lipoprotein Triglyceride Overproduction And Low Density Lipoprotein Hypercatabolism, Soonkyu Chung, Jenelle M. Timmins, MyNgan Duong, Chiara Degirolamo, Shunxing Rong, Janet K. Sawyer, Roshni R. Singaraja, Michael R. Hayden, Nobuyo Maeda, Lawrence L. Rudel, Gregory S. Shelness, John S. Parks 2010 Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL

Targeted Deletion Of Hepatocyte Abca1 Leads To Very Low Density Lipoprotein Triglyceride Overproduction And Low Density Lipoprotein Hypercatabolism, Soonkyu Chung, Jenelle M. Timmins, Myngan Duong, Chiara Degirolamo, Shunxing Rong, Janet K. Sawyer, Roshni R. Singaraja, Michael R. Hayden, Nobuyo Maeda, Lawrence L. Rudel, Gregory S. Shelness, John S. Parks

Nutrition and Health Sciences -- Faculty Publications

Loss of ABCA1 activity in Tangier disease (TD) is associated with abnormal apoB lipoprotein (Lp) metabolism in addition to the complete absence of high density lipoprotein (HDL). We used hepatocyte-specific ABCA1 knock-out (HSKO) mice to test the hypothesis that hepatic ABCA1 plays dual roles in regulating Lp metabolism and nascent HDL formation. HSKO mice recapitulated the TD lipid phenotype with postprandial hypertriglyceridemia, markedly decreased LDL, and near absence of HDL. Triglyceride (TG) secretion was 2-fold higher in HSKO compared with wild type mice, primarily due to secretion of larger TG-enriched VLDL secondary to reduced hepatic phosphatidylinositol 3-kinase signaling. HSKO mice ...


Combined Therapy Of Dietary Fish Oil And Stearoyl-Coa Desaturase 1 Inhibition Prevents The Metabolic Syndrome And Atherosclerosis, J. Mark Brown, Soonkyu Chung, Janet K. Sawyer, Chiara Degirolamo, Heather M. Alger, Tam M. Nguyen, Xuewei Zhu, My-Ngan Duong, Amanda L. Brown, Caleb Lord, Ramesh Shah, Matthew A. Davis, Kathryn Kelley, Martha D. Wilson, Jennifer Madenspacher, Michael B. Fessler, John S. Parks, Lawrence L. Rudel 2010 Wake Forest University School of Medicine

Combined Therapy Of Dietary Fish Oil And Stearoyl-Coa Desaturase 1 Inhibition Prevents The Metabolic Syndrome And Atherosclerosis, J. Mark Brown, Soonkyu Chung, Janet K. Sawyer, Chiara Degirolamo, Heather M. Alger, Tam M. Nguyen, Xuewei Zhu, My-Ngan Duong, Amanda L. Brown, Caleb Lord, Ramesh Shah, Matthew A. Davis, Kathryn Kelley, Martha D. Wilson, Jennifer Madenspacher, Michael B. Fessler, John S. Parks, Lawrence L. Rudel

Nutrition and Health Sciences -- Faculty Publications

Background—Stearoyl-CoA desaturase 1 (SCD1) is a critical regulator of energy metabolism and inflammation. We have previously reported that inhibition of SCD1 in hyperlipidemic mice fed a saturated fatty acid (SFA)-enriched diet prevented development of the metabolic syndrome, yet surprisingly promoted severe atherosclerosis. In this study we tested whether dietary fish oil supplementation could prevent the accelerated atherosclerosis caused by SCD1 inhibition.

Methods and Results—LDLr-/-, ApoB100/100 mice were fed diets enriched in saturated fat or fish oil in conjunction with antisense oligonucleotide (ASO) treatment to inhibit SCD1. As previously reported, in SFA-fed mice, SCD1 inhibition dramatically ...


Nutrition-Related Practices And Attitudes Of Kansas Skipped-Generation(S) Caregivers And Their Grandchildren, Mary Meck Higgins, Bethany J. Murray 2010 Kansas State University

Nutrition-Related Practices And Attitudes Of Kansas Skipped-Generation(S) Caregivers And Their Grandchildren, Mary Meck Higgins, Bethany J. Murray

Nutrition and Health Sciences -- Faculty Publications

Despite growing numbers, the nutrition practices and attitudes of skipped-generation(s) kinship caregivers regarding feeding the dependent children in their care have not been examined. In this qualitative study, transcriptions of semi-structured interviews with 19 female and four male skipped-generation(s) Kansas caregivers (ages 47 to 80, 92% non-Hispanic whites, 83% female, 78% grandparents and 22% great-aunt or great-grandparent caregivers; caring for a range of one to four children, ages three to 18, for an average of nine years) were content analyzed for how their nutrition-related practices and attitudes had changed since parenting the first time. Sub-themes regarding practices included ...


Liver Injury In Acute Fatty Liver Of Pregnancy: Possible Link To Placental Mitochondrial Dysfunction And Oxidative Stress, Sathish Kumar Natarajan, Kavitha R. Thangaraj, C. E. Eapen, Anup Ramachandran, Ashis Mukhopadhya, Mathews Mathai, Lakshmi Seshadri, Abraham Peedikayil, Banumathi Ramakrishna, Kunnissery A. Balasubramanian 2010 Christian Medical College

Liver Injury In Acute Fatty Liver Of Pregnancy: Possible Link To Placental Mitochondrial Dysfunction And Oxidative Stress, Sathish Kumar Natarajan, Kavitha R. Thangaraj, C. E. Eapen, Anup Ramachandran, Ashis Mukhopadhya, Mathews Mathai, Lakshmi Seshadri, Abraham Peedikayil, Banumathi Ramakrishna, Kunnissery A. Balasubramanian

Nutrition and Health Sciences -- Faculty Publications

Acute fatty liver of pregnancy (AFLP) is a rare disorder which is fatal if not recognized and treated early. Delivery of the feto-placental unit results in dramatic improvement in maternal liver function, suggesting a role for the placenta. However, the mechanisms by which defects in the fetus or placenta lead to maternal liver damage are not well understood and form the focus of this study. Placenta and serum were obtained at delivery from patients with AFLP, and placental mitochondria and peroxisomes were isolated. Placental mitochondrial function, oxidative stress, and fatty acid composition as well asserumantioxidants, oxidativeandnitrosative stress markers,andfatty acid ...


Predicting Successful Aging In A Population-Based Sample Of Georgia Centenarians, Jonathan Arnold, Jianliang Dai, Lusine Nahapetyan, Ankit Arte, Mary Ann Johnson, Dorothy Hausman, Willard L. Rodgers, Robert Hensley, Peter Martin, Maurice MacDonald, Adam Davey, Ilene C. Siegler, S. Michal Jazwinski, Leonard W. Poon 2010 University of Georgia

Predicting Successful Aging In A Population-Based Sample Of Georgia Centenarians, Jonathan Arnold, Jianliang Dai, Lusine Nahapetyan, Ankit Arte, Mary Ann Johnson, Dorothy Hausman, Willard L. Rodgers, Robert Hensley, Peter Martin, Maurice Macdonald, Adam Davey, Ilene C. Siegler, S. Michal Jazwinski, Leonard W. Poon

Nutrition and Health Sciences -- Faculty Publications

Used a population-based sample (Georgia Centenarian Study, GCS), to determine proportions of centenarians reaching 100 years as (1) survivors (43%) of chronic diseases first experienced between 0–80 years of age, (2) delayers (36%) with chronic diseases first experienced between 80–98 years of age, or (3) escapers (17%) with chronic diseases only at 98 years of age or older. Diseases fall into two morbidity profiles of 11 chronic diseases; one including cardiovascular disease, cancer, anemia, and osteoporosis, and another including dementia. Centenarians at risk for cancer in their lifetime tended to be escapers (73%), while those at risk for ...


Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek 2009 The University of Tennessee

Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek

Seung J Baek

Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- ...


Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun 2009 The University of Tennessee

Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun

Seung J Baek

Capsaicin, a natural product of the Capsicum species of red peppers, is known to induce apoptosis and suppress growth. Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and antitumorigenic property in colorectal and lung cancer. Our data demonstrate that capsaicin leads to induction of apoptosis and up-regulates NAG-1 gene expression at the transcriptional level. Overexpression of CCAAT/enhancer binding protein beta (C/EBPbeta) caused a significant increase of basal and capsaicin-induced NAG-1 promoter activity. We subsequently identified C/EBPbeta binding sites in the NAG-1 promoter responsible for capsaicin-induced NAG-1 transactivation. Electrophoretic mobility shift assay and chromatin immunoprecipitation ...


A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, CK Choi, A English, JS Kim 2009 The University of Tennessee

A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, Ck Choi, A English, Js Kim

Seung J Baek

The main aims of this study were to elucidate the effect of green tea catechins on Nudix-type motif 6 (NUDT6) suppression and to characterize NUDT6's biological activity. Our microarray data showed that the green tea component epicatechin-3-gallate suppressed NUDT6 expression, and this was confirmed by RT-PCR. Subsequently, the use of different catechins showed that the effect of epigallocatechin-3-gallate (EGCG) was stronger than that of other catechins. At the posttranscriptional level, EGCG decreased the RNA stability of NUDT6, indicating it as a potential mechanism of NUDT6 suppression. Further cloning of the 3' untranslated region of human NUDT6 mRNA resulted in ...


In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek 2009 The University of Tennessee

In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek

Seung J Baek

Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment of cancer, arthritis and diabetes mellitus. The purpose of this study was to determine the molecular mechanisms by which this plant shows beneficial effects. An 80% ethanolic extract of C. fenestratum (80ET) was separated by its polarity into dichloromethane (DCM) and aqueous fractions (WF), and the anti-proliferative effects of 80ET, DCM and WF were investigated. Berberine, one of the major components of C. fenestratum, was used as a control. The 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ...


Digital Commons powered by bepress