Effects Of Cadherin Mediated Contact Normalization On Oncogenic Src Kinase Mediated Gene Expression And Protein Phosphorylation,
2024
Rowan University
Effects Of Cadherin Mediated Contact Normalization On Oncogenic Src Kinase Mediated Gene Expression And Protein Phosphorylation, Rachel E Nicoletto, Cayla J Holdcraft, Ariel C Yin, Edward P Retzbach, Stephanie A Sheehan, Amanda A Greenspan, Christopher M Laugier, Jason Trama, Caifeng Zhao, Haiyan Zheng, Gary S Goldberg
Rowan-Virtua School of Osteopathic Medicine Departmental Research
Nontransformed cells form heterotypic cadherin junctions with adjacent transformed cells to inhibit tumor cell growth and motility. Transformed cells must override this form of growth control, called "contact normalization", to invade and metastasize during cancer progression. Heterocellular cadherin junctions between transformed and nontransformed cells are needed for this process. However, specific mechanisms downstream of cadherin signaling have not been clearly elucidated. Here, we utilized a β-catenin reporter construct to determine if contact normalization affects Wnt signaling in transformed cells. β-catenin driven GFP expression in Src transformed mouse embryonic cells was decreased when cultured with cadherin competent nontransformed cells compared to …
Unravelling The Impact Of Blood Metabolites, And Lifestyle Factors On Periodontal Disease Using Mendelian Randomization,
2024
University of South Florida
Unravelling The Impact Of Blood Metabolites, And Lifestyle Factors On Periodontal Disease Using Mendelian Randomization, Rhea Charles
USF Tampa Graduate Theses and Dissertations
Periodontal disease remains a global public health concern. Despite the availability of preventive and therapeutic strategies, the multifactorial nature of periodontitis complicates its understanding and management. Periodontal disease is associated with an increased risk of chronic conditions, including ischemic heart disease (IHD), gestational hypertension, respiratory diseases such as COPD and bronchitis, and various cancers, including kidney and pancreatic cancers. Moreover, individuals with diabetes, autoimmune diseases like lupus and Crohn’s disease, and osteoporosis are more likely to develop periodontitis. The ambiguity in the direction of causality between periodontal disease and its associated conditions poses challenges for effective treatment. Mendelian randomization offers …
The Short Allele Of The Serotonin Transporter Gene (Slc6a4) Increases Predisposition To Anxiety And Negative Emotional States,
2024
University of North Florida
The Short Allele Of The Serotonin Transporter Gene (Slc6a4) Increases Predisposition To Anxiety And Negative Emotional States, Gabriella Hitti
PANDION: The Osprey Journal of Research and Ideas
Mental disorders, such as anxiety and mood disorders, have long been a focus of psychological research, and increasing evidence points to a genetic basis for their occurrence. Polymorphisms of the serotonin transporter (5-HTT) gene (SLC6A4) may predispose an individual to anxiety-related symptoms. SLC6A4 has two common alleles: the short (s) allele, which results in less 5-HTT protein production, and the long (l) allele, which results in more 5-HTT. Integrated findings from psychometric evaluations, behavioral animal models, and biological assessments establish a link between the s allele of SLC6A4 and heightened anxiety phenotypes. The allele’s influence on serotonin levels and brain …
Sars-Cov-2 Vaccine Improved Hemostasis Of A Patient With Protein S Deficiency: A Case Report,
2024
LSU Health Sciences Center - New Orleans
Sars-Cov-2 Vaccine Improved Hemostasis Of A Patient With Protein S Deficiency: A Case Report, Mohammad A. Mohammad, Alaa Malik, Lekha Thangada, Diana Polanía-Villanueva, Jovanny Zabaleta, Rinku Majumder
School of Medicine Faculty Publications
A 16-year-old patient, while an infant, incurred right-sided hemiparesis and had difficulty breast feeding. She was later diagnosed with a neonatal stroke and her genetic testing showed a missense mutation in her PROS1 (Protein S) gene. Both her grandfather and father, but not her mother, had hereditary Protein S (PS) deficiency. The patient was not prescribed any mediation due to her young age but was frequently checked by her physician. The patient’s plasma was first collected at the age of 13, and the isolated plasma from the patient and her father were analyzed by aPTT, thrombin generation, and enzyme-linked immunosorbent …
Analyses Of Whole-Genome
Sequences From 185 North
American Thoroughbred Horses,
Spanning 5 Generations,
2024
University of Kentucky
Analyses Of Whole-Genome Sequences From 185 North American Thoroughbred Horses, Spanning 5 Generations, Ernie Bailey, Carrie J. Finno, Jonah N. Cullen, Ted Kalbfleisch, Jessica Lynn Petersen
Department of Animal Science: Faculty Publications
Whole genome sequences (WGS) of 185 North American Thoroughbred horses were compared to quantify the number and frequency of variants, diversity of mitotypes, and autosomal runs of homozygosity (ROH). Of the samples, 82 horses were born between 1965 and 1986 (Group 1); the remaining 103, selected to maximize pedigree diversity, were born between 2000 and 2020 (Group 2). Over 14.3 million autosomal variants were identified with 4.5–5.0 million found per horse. Mitochondrial sequences associated the North American Thoroughbreds with 9 of 17 clades previously identified among diverse breeds. Individual coefficients of inbreeding, estimated from ROH, averaged 0.266 (Group 1) and …
Cross-Species Analysis Of Rpl24 Knockout For Cftr Function Restoration,
2024
University of Alabama at Birmingham
Cross-Species Analysis Of Rpl24 Knockout For Cftr Function Restoration, Brittany Jackson, Ryan Mancinone, John L. Hartman Iv
Expo Student Presentations
2024 Fall Expo Poster Presentation
Monitoring The Seasonal Presence Of The Blue Shiner, Cyprinella Caerulea, In Little River Canyon National Preserve Using Environmental Dna,
2024
Jacksonville State University
Monitoring The Seasonal Presence Of The Blue Shiner, Cyprinella Caerulea, In Little River Canyon National Preserve Using Environmental Dna, Deanna Meadows
Theses
The study of environmental DNA (eDNA) has provided researchers with a more accessible and sensitive way to identify the presence of specific species compared to traditional monitoring methods. eDNA enables species detection by analyzing environmental samples such as water or soil, which contain genetic material shed by organisms in a given area. Research indicates that eDNA techniques have become a valuable method for monitoring threatened and invasive species, proving particularly reliable for detecting aquatic species compared to traditional techniques. Quantitative PCR (qPCR) has been used in numerous eDNA studies, as it provides for greater accuracy than conventional PCR. Cyprinella …
Characterization Of Developmentally Downregulated Nfe2l3 In Retinal Ganglion Cell Survival And Axon Regeneration,
2024
University Of Connecticut
Characterization Of Developmentally Downregulated Nfe2l3 In Retinal Ganglion Cell Survival And Axon Regeneration, Lucy Homer
Holster Scholar Projects
Retinal ganglion cells (RGCs) are central nervous system projection neurons essential for transmitting visual information from the eye to the brain. Damage to RGCs, caused by conditions such as glaucoma and traumatic optic neuropathy (TON), may lead to vision loss or blindness, as adult RGCs lack regenerative capacity. This study focuses on Nfe2l3 (Nrf3), a developmentally downregulated transcription factor known to mediate cellular responses to oxidative stress, a key pathological event following optic nerve injury. We hypothesized that overexpressing Nfe2l3 in RGCs would enhance their survival and promote axon regeneration after optic nerve crush (ONC) injury. To test this, we …
Upregulation Of Fatty Acid Synthase Increases Activity Of Β-Catenin And Expression Of Notum To Enhance Stem-Like Properties Of Colorectal Cancer Cells,
2024
University of Kentucky
Upregulation Of Fatty Acid Synthase Increases Activity Of Β-Catenin And Expression Of Notum To Enhance Stem-Like Properties Of Colorectal Cancer Cells, Courtney O. Kelson, Josiane Weber Tessmann, Mariah E. Geisen, Daheng He, Chi Wang, Tianyan Gao, B. Mark Evers, Yekaterina Y. Zaytseva
Markey Cancer Center Faculty Publications
Dysregulated fatty acid metabolism is an attractive therapeutic target for colorectal cancer (CRC). We previously reported that fatty acid synthase (FASN), a key enzyme of de novo synthesis, promotes the initiation and progression of CRC. However, the mechanisms of how upregulation of FASN promotes the initiation and progression of CRC are not completely understood. Here, using Apc/VillinCre and ApcMin mouse models, we show that upregulation of FASN is associated with an increase in activity of β-catenin and expression of multiple stem cell markers, including Notum. Genetic and pharmacological downregulation of FASN in mouse adenoma organoids decreases the activation of β-catenin …
Inorganic Pyrophosphate Plasma Levels In Patients With Ggcx-Associated Pxe-Like Phenotypes,
2024
Thomas Jefferson University
Inorganic Pyrophosphate Plasma Levels In Patients With Ggcx-Associated Pxe-Like Phenotypes, Qiaoli Li, Catherine Troutman, Mary Peckiconis, Tamara Wurst, Sharon Terry
Department of Biochemistry and Molecular Biology Faculty Papers
ntroduction: Pseudoxanthoma elasticum (PXE) is an autosomal recessive ectopic calcification disorder clinically affecting the skin, eyes, and vascular system. Most cases of PXE are caused by inactivating pathogenic variants in the ABCC6 gene encoding a hepatic transmembrane efflux transporter, which facilitates the extracellular release of ATP, the precursor of inorganic pyrophosphate (PPi), a potent endogenous inhibitor of calcification. Pathogenic variants in GGCX, encoding γ-glutamyl carboxylase required for activation of vitamin K-dependent coagulation factors as well as matrix Gla protein (MGP) and Gla-rich protein (GRP), two inhibitors of ectopic calcification, have also been reported to cause cutaneous changes like those seen …
Effects Of Microplastic Biofilms On An Anthropogenically Impacted Suburban Lake,
2024
Grand Valley State University
Effects Of Microplastic Biofilms On An Anthropogenically Impacted Suburban Lake, Paris M. Velasquez
Masters Theses
Plastics have been observed in every location on the planet, and their prevalence in the environment is due in part to their strong resistance to degradation. Inland lakes are susceptible to plastic pollution by highway runoff, which contains plastic fragments of brake pads, car tires, litter, and road paint. These plastics eventually enter freshwater environments and degrade into microplastics (
Use Of A Novel Combination Of Multiplex Pcr And Dna Barcoding In Assessing Authenticity Of Ginseng Products,
2024
Chapman University
Use Of A Novel Combination Of Multiplex Pcr And Dna Barcoding In Assessing Authenticity Of Ginseng Products, Diane Y. Kim, Donna Miranda-Romo, Adriana R. Ten Cate, Rosalee S. Hellberg
Food Science Faculty Articles and Research
Ginseng (Panax sp.) is a medicinal plant used for its purported health benefits, primarily in East Asian countries. The COVID-19 pandemic led to a significant increase in sales of dietary supplements, including ginseng supplements, to purportedly support immune health and provide other health benefits. However, this heightened demand has subsequently increased the risk of adulteration in these dietary supplements. This study aimed to determine the efficacy of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing Panax ginseng, Panax quinquefolius, or Panax notoginseng were obtained …
Animal Breeding-Rcn: Farm Animal Genomics Collective,
2024
Utah State University
Animal Breeding-Rcn: Farm Animal Genomics Collective, Noelle E. Cockett
Funded Research Records
No abstract provided.
Deciphering Evolutionary Co-Option By Studying The Origin Of Self-Fertility In Caenorhabditis Nematodes,
2024
Rowan University
Deciphering Evolutionary Co-Option By Studying The Origin Of Self-Fertility In Caenorhabditis Nematodes, Jonathan P. Harbin
Theses and Dissertations
In my research, I examined the characteristics of male and female sex-determination mutants in C. nigoni and investigated the regulatory pathway they define. This work tested whether flexibility in the sex-determination pathway was a preexisting condition that favored the origin of self-fertility in Caenorhabditis. Furthermore, I developed an approach for using interspecies hybrid mutants to assess the robustness of the C. nigoni pathway. My findings showed that the C. nigoni pathway is highly robust and canalized, suggesting that changes leading to self-fertility must have involved the impairment of this canalization in the germ line, to allow eventual alteration of germ …
Exploring Genetic Elements Related To The Co-Occurrence Of Crohn’S Disease And Rheumatoid Arthritis,
2024
Nova Southeastern University
Exploring Genetic Elements Related To The Co-Occurrence Of Crohn’S Disease And Rheumatoid Arthritis, Vamsi Korisapati, Karam Asad, Andrew T. Ozga
Mako: NSU Undergraduate Student Journal
Crohn’s disease (CD) is a rare inflammatory bowel disease that negatively impacts the lining of the digestive tract, while Rheumatoid Arthritis (RA) is an autoimmune disorder wherein the body attacks healthy cells, also causing inflammation. The primary cause of inflammation in both diseases is an overactive immune response, but patients who are diagnosed with RA are typically prescribed nonsteroidal anti-inflammatory drugs, which is not a suggested method for treating CD. Here we do a literature survey through PubMed to uncover genetic variants common to both diseases and highlighted four shared genes: HLA-DRB1, NOD2, CARD9, and CXCL10. We then examined these …
Cytotoxic Potential Of Fungi In The Genus Ganoderma,
2024
Nova Southeastern University
Cytotoxic Potential Of Fungi In The Genus Ganoderma, Abril Chao, Omobolanle A. Ayangade, Braham Dhillon, Navi Gill
Mako: NSU Undergraduate Student Journal
Ganoderma lucidum is a mushroom forming fungus that has been used in traditional Chinese medicine for over 2,000 years. G. lucidum, also known as lingzhi or reishi, is consumed in several Asian cultures to promote health and longevity. Bioactive molecules with anticancer properties have been isolated and characterized primarily from the species Ganoderma lucidum, and the cytotoxic potential of other species in the genus Ganoderma remains relatively unclear. Bioactive molecules isolated from mushroom fruiting bodies are demonstrated to have cytotoxicity against different cancer cell lines. Triterpenes and polysaccharides are the two major groups of compounds that exhibit antitumor and anti-inflammatory …
The Impact Of Temperature And Humidity On The Transcriptome Of Symbiochloris Reticulata In Relation To Lobaria Pulmonaria’S Genepools,
2024
Biological Research Center, “Babeș-Bolyai” University, Jibou, Romania
The Impact Of Temperature And Humidity On The Transcriptome Of Symbiochloris Reticulata In Relation To Lobaria Pulmonaria’S Genepools, Ioana Violeta Ardelean, Christoph Scheidegger, Mihai Miclaus
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
Onsite Microbial Analyses In One Of The Most Remote Quartzite Subterranean Environment On Earth: Imawarì Yeuta On Venezuelan Tepuis,
2024
University of Bologna
Onsite Microbial Analyses In One Of The Most Remote Quartzite Subterranean Environment On Earth: Imawarì Yeuta On Venezuelan Tepuis, Martina Cappelletti
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
Enhanced Bmp Signaling Via Alk2 In Osteoclasts Decreases Bone Density In Mice,
2024
University of Texas Rio Grande Valley
Enhanced Bmp Signaling Via Alk2 In Osteoclasts Decreases Bone Density In Mice, Yolanda V. Gutierrez, Hiroyuki Yamaguchi, Yuji Mishina, Yoshihiro Komatsu
Research Colloquium
Bone remodeling is a complex biological process that has been extensively studied. Bone Morphogenetic Proteins (BMPs) are recognized as one of the critical growth factors that coordinate bone remodeling. Previous studies have demonstrated that BMP signaling in osteoclasts has a positive effect on osteoclast function. However, little is known about how each BMP type I receptors control osteoclastogenesis. To investigate this question, we utilized the Cre-LoxP system to specifically activate BMP signaling through ALK2 in mice. We utilized Cathepsin K (Ctsk)-Cre driver to activate BMP signaling in osteoclasts in mice. Compared with aged- and gender-matched controls, gain-of-function of BMP mutant …
Antarctic Glacier Melting Modeling Distribution Of Bacterial Community Structure,
2024
Institute of Biology Bucharest of the Romanian Academy, Bucharest, Romania
Antarctic Glacier Melting Modeling Distribution Of Bacterial Community Structure, Corina Itcus, Constantin Marin, Soon Gyu Hong, Manuela Elisabeta Sidoroff, Cristian Coman, Cristina Purcarea
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
