Mtbindingsim: Simulate Protein Binding To Microtubules,
2012
University of Notre Dame
Mtbindingsim: Simulate Protein Binding To Microtubules, Julia T. Philip, Charles H. Pence, Holly V. Goodson
Faculty Publications
Summary: Many protein–protein interactions are more complex than can be accounted for by 1:1 binding models. However, biochemists have few tools available to help them recognize and predict the behaviors of these more complicated systems, making it difficult to design experiments that distinguish between possible binding models. MTBindingSim provides researchers with an environment in which they can rapidly compare different models of binding for a given scenario. It is written specifically with microtubule polymers in mind, but many of its models apply equally well to any polymer or any protein–protein interaction. MTBindingSim can thus both help in training intuition about …
Dna Secondary Structures And Their Contribution To Mutagenesis In B. Subtilis Stationary Phase Cells,
2012
University of Nevada, Las Vegas
Dna Secondary Structures And Their Contribution To Mutagenesis In B. Subtilis Stationary Phase Cells, Carmen Vallin, Holly Anne Martin, Christian Ross, Ronald Yasbin, Eduardo Robleto
McNair Poster Presentations
It is widely known and accepted that the cause of many mutations in cells are generated during the replication process of actively dividing cells, however more recent research has shown that mutations also arise in non growing conditions, a phenomenon known as stationary phase mutagenesis. Much of what is known come from studies in eukaryotic and bacterial models. It has been proposed that in non-growing cells, the process of transcription plays an important role in mutagenesis. We test the hypothesis that DNA secondary structures, formed during transcription, promote mutagenesis. The transcription-generated structures are speculated to be prone to by blocking …
Transcriptome Analysis Of Glue Secretion In Drosophila,
2012
University of Nevada, Las Vegas
Transcriptome Analysis Of Glue Secretion In Drosophila, William Mccurdy, Pawel Parafianowicz, Andrew Andres
McNair Poster Presentations
Steroid hormones control important developmental and physiological responses in animals, including humans. It is known that when a cell is exposed to a steroid hormone, there is an immediate change in the genes that are expressed into proteins. Of notable importance is steroid regulation in the salivary glands of larval Drosophila melanogaster and the corresponding physiological responses that are governed by treatment with the conserved insect steroid, 20-hydroxyecdysone (20E). Exposure to the steroid hormone 20E causes a change in gene expression that facilitates the secretion of glue glycoproteins from inside the cells into the lumen of the tissue. Altered gene …
Pando Clone (World's Largest Living Organism?) History And Evaluation : Case Study For Revalidation,
2012
Utah State University
Pando Clone (World's Largest Living Organism?) History And Evaluation : Case Study For Revalidation, Allen V. Henningson
Aspen Bibliography
No abstract provided.
Decision Memo: Pando Aspen Clone Restoration Project,
2012
Utah State University
Decision Memo: Pando Aspen Clone Restoration Project, Kurt Robins
Aspen Bibliography
The Pando Clone is located in Township 26 South, Range 1 East, Sections 24 and 25 about 1 mile southwest of the Lakeside Resort on State Highway 25 and about 1 mile due east of Mallard Bay at the southwest corner of Fish Lake (see map). The Pando Clone is the largest aspen clone in the world and the largest (most massive) single living organism in existence that has been discovered to date (Grant, 1993; DeWoody et al, 2008). It encompasses approximately 106 acres and is estimated to weigh in excess of 13 million pounds (Grant, 1993). This clone is …
Patents Fettering Reproductive Rights,
2012
Indiana University Maurer School of Law
Patents Fettering Reproductive Rights, Scott A. Allen
Indiana Law Journal
No abstract provided.
Characterization Of Intracellular Interactions Between Dengue Virus And Host Proteins,
2012
Wayne State University
Characterization Of Intracellular Interactions Between Dengue Virus And Host Proteins, Dumrong Mairiang
Wayne State University Dissertations
Dengue virus is the causative agent of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of world population live in areas where dengue is prevalent, leading to high levels of morbidity and mortality in many areas. Currently there are no vaccines or effective treatments. The virus is transmitted from one person to another by the yellow fever mosquito, Aedes aegypti. The genome of dengue virus encodes only ten proteins implying that the virus needs to interact with and utilize several host proteins for replication. In this project, I used high-throughput yeast two-hybrid screening to identify mosquito and …
Contribution Of The Infection-Associated Complement Regulator-Acquiring Surface Protein 4 (Erpc) To Complement Resistance Of Borrelia Burgdorferi,
2012
Frankfurt University Hospital, Germany
Contribution Of The Infection-Associated Complement Regulator-Acquiring Surface Protein 4 (Erpc) To Complement Resistance Of Borrelia Burgdorferi, Claudia Hammerschmidt, Teresia Hallström, Christine Skerka, Reinhard Wallich, Brian Stevenson, Peter F Zipfel, Peter Kraiczy
Microbiology, Immunology, and Molecular Genetics Faculty Publications
Borrelia burgdorferi evades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contribution of CRASP-4 in mediating complement resistance of B. burgdorferi and its interaction with human complement regulators. CRASP-4 (also known as ErpC) was immobilized onto magnetic beads and used to capture proteins from human serum. Following Western blotting, factor H (CFH), CFH-related protein 1 (CFHR1), CFHR2, and CFHR5 were identified as ligands of CRASP-4. To analyze the impact of native CRASP-4 on mediating survival of serum-sensitive cells in human serum, a B. garinii strain was generated …
"Release Factor 2 (Rf2) Is A Key Structural Component Found In The Ribosomes Of All Prokaryotic Organisms…",
2012
University of Alabama in Huntsville
"Release Factor 2 (Rf2) Is A Key Structural Component Found In The Ribosomes Of All Prokaryotic Organisms…", Zachary Wise
Summer Community of Scholars Posters (RCEU and HCR Combined Programs)
No abstract provided.
The Role Of Chromatin And Cofactors In The Transcriptional Memory Effect Exerted In Saccharomyces Cerevisiae,
2012
University at Albany, State University of New York
The Role Of Chromatin And Cofactors In The Transcriptional Memory Effect Exerted In Saccharomyces Cerevisiae, Emily Leigh Paul
Legacy Theses & Dissertations (2009 - 2024)
Abf1 and Rap1 are functionally similar general regulatory factors (GRFs) found in Saccharomyces cerevisiae . Abf1, in its role as a transcriptional activator, exerts a memory effect on some genes under its control. This effect results in transcription levels remaining steady when Abf1 dissociates from its binding site in a conditional mutant. In contrast, Rap1 fails to elicit the same effect on its regulatory targets. Transcriptional memory effects have been observed in many fields of study, including immunology, cancer, and stem cells, and conservation of transcription machinery will allow studies in yeast to be applied to higher organisms.
The Role Of Ess1 In Survival, Morphogenetic Switching And Transcription In The Fungal Pathogen Candida Albicans,
2012
University at Albany, State University of New York
The Role Of Ess1 In Survival, Morphogenetic Switching And Transcription In The Fungal Pathogen Candida Albicans, Dhanushki Poornima Samaranayake
Legacy Theses & Dissertations (2009 - 2024)
Candida albicans is a fungal pathogen that causes serious infections among immune-compromised patients and premature infants. C. albicans can become drug resistant, therefore, identifying new antifungal drug targets is an important goal. Here, we study a peptidyl-prolyl cis/trans isomerase called Ess1 as a potential drug target. Ess1 is conserved among pathogenic fungi, and therefore, potential inhibitors of Ess1 should display a broad spectrum of activity. We confirm that Ess1 is essential for growth in Candida albicans, but unlike the previously published find, deleting one copy of the C. albicans ESS1 gene did not affect morphogenetic switching. However, further reducing activity …
An Unbiased Approach To Identify Genes Involved In Development In A Turtle With Temperature-Dependent Sex Determination,
2012
University of South Carolina - Beaufort
An Unbiased Approach To Identify Genes Involved In Development In A Turtle With Temperature-Dependent Sex Determination, Jena L. Chojnowski, Edward L. Braun
Natural Sciences Faculty Publications
Many reptiles exhibit temperature-dependent sex determination (TSD). The initial cue in TSD is incubation temperature, unlike genotypic sex determination (GSD) where it is determined by the presence of specific alleles (or genetic loci). We used patterns of gene expression to identify candidates for genes with a role in TSD and other developmental processes without making a priori assumptions about the identity of these genes (ortholog-based approach). We identified genes with sexually dimorphic mRNA accumulation during the temperature sensitive period of development in the Red-eared slider turtle (Trachemys scripta), a turtle with TSD. Genes with differential mRNA accumulation in response to …
Consuming Direct-To-Consumer Genetic Tests: The Role Of Genetic Literacy And Knowledge Calibration,
2012
Old Dominion University
Consuming Direct-To-Consumer Genetic Tests: The Role Of Genetic Literacy And Knowledge Calibration, Yvette E. Pearson, Yuping Liu-Thompkins
Philosophy Faculty Publications
As direct-to-consumer marketing of medical genetic tests grows in popularity, there is an increasing need to better understand the ethical and public policy implications of such products. The complexity of genetic tests raises serious concerns about whether consumers possess the knowledge to make sound decisions about their use. This research examines the effects of educational intervention and feedback on consumers' genetic literacy and calibration -- the gap between consumers' actual knowledge and how much they think they know. The authors find that consumers' genetic knowledge was generally low and that people tended to underestimate their knowledge level. Furthermore, consumers' perceived …
Dynamics Of Envelope Evolution In Clade C Shiv-Infected
Pig-Tailed Macaques During Disease Progression
Analyzed By Ultra-Deep Pyrosequencing,
2012
University of Nebraska-Lincoln
Dynamics Of Envelope Evolution In Clade C Shiv-Infected Pig-Tailed Macaques During Disease Progression Analyzed By Ultra-Deep Pyrosequencing, For Yue Tso, Damien C. Tully, Sandra Gonzalez, Christopher Quince, On Ho, Patricia Polacino, Ruth M. Ruprecht, Shiu-Lok Hu, Charles Wood
Nebraska Center for Virology: Faculty Publications
Understanding the evolution of the human immunodeficiency virus type 1 (HIV-1) envelope during disease progression can provide tremendous insights for vaccine development, and simian-human immunodeficiency virus (SHIV) infection of nonhuman primate provides an ideal platform for such studies. A newly developed clade C SHIV, SHIV-1157ipd3N4, which was able to infect rhesus macaques, closely resembled primary HIV-1 in transmission and pathogenesis, was used to infect several pig-tailed macaques. One of the infected animals subsequently progressed to AIDS, whereas one remained a nonprogressor. The viral envelope evolution in the infected animals during disease progression was analyzed by a bioinformatics approach using ultra-deep …
Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes,
2012
Wayne State University
Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes, Olga Astapova
Wayne State University Dissertations
PPAR-gamma is a nuclear receptor that plays a central role in metabolic regulation by regulating extensive gene expression networks in adipose, liver, skeletal muscle and many other tissues. Human PPAR-gamma mutations are rare and cause a monogenetic form of severe type II diabetes with metabolic syndrome, known as familiar partial lypodystrophy. The E157D PPAR-gamma mutant causes atypical lipodystrophy in a large Canadian kindred, presenting with multiple musculoskeletal, neurological and hematological abnormalities in addition to the classic lipodystrophy features of insulin-resistant diabetes, hypertension and dyslipidemia. This mutation is localized to the p-box of PPAR-gamma, a small region that interacts directly with …
Genetics And Cancer,
2012
Parkland College
Genetics And Cancer, Sachin Puri
A with Honors Projects
Genes' effect in body and relationship with cancer. Role in cell cycle and angiogenesis.
Genome Sequence Of Strain Himb30, A Novel Member Of The Marine Gammaproteobacteria,
2012
Edith Cowan University
Genome Sequence Of Strain Himb30, A Novel Member Of The Marine Gammaproteobacteria, Megan Huggett, Michael Rappe
Research outputs 2012
Strain HIMB30 was isolated from coastal Hawaii seawater by extinction culturing in seawater-based oligotrophic medium. It is a phylogenetically unique member of the class Gammaproteobacteria that is only distantly related to its closest cultured relatives. Here we present the genome sequence of strain HIMB30, including genes for proteorhodopsin-based phototrophy and the Calvin-Benson-Bassham cycle.
Genome Sequence Of Strain Himb55, A Novel Marine Gammaproteobacterium Of The Om60/Nor5 Clade,
2012
Edith Cowan University
Genome Sequence Of Strain Himb55, A Novel Marine Gammaproteobacterium Of The Om60/Nor5 Clade, Megan Huggett, Michael Rappe
Research outputs 2012
Strain HIMB55 is a phylogenetically unique member of the OM60/NOR5 clade of the Gammaproteobacteria isolated from coastal seawater of Kaneohe Bay on the northeastern shore of Oahu, Hawaii, by extinction culturing in seawater-based oligotrophic medium. Here we present the genome sequence of strain HIMB55, including genes for bacteriochlorophyll-based phototrophy.
Genome Sequence Of Strain Himb624, A Cultured Representative From The Om43 Clade Of Marine Betaproteobacteria,
2012
Edith Cowan University
Genome Sequence Of Strain Himb624, A Cultured Representative From The Om43 Clade Of Marine Betaproteobacteria, Megan Huggett, Darren Hayakawa, Michael Rappe
Research outputs 2012
Strain HIMB624 is a planktonic marine bacterium within the family Methylophilaceae of the class Betaproteobacteria isolated from coastal seawater of Oahu, Hawaii. This strain is of interest because it is one of few known isolates from an abundant clade of Betaproteobacteria found in cultivation-independent studies of coastal seawater and freshwater environments around the globe, known as OM43. Here we describe some preliminary features of the organism, draft genome sequence and annotation, and comparative genomic analysis with one other sequenced member of this clade (strain HTCC2181). The 1,333,209 bp genome of strain HIMB624 is arranged in a single scaffold containing four …
Protein Structure Networks,
2012
Old Dominion University
Protein Structure Networks, Lesley H. Greene
Chemistry & Biochemistry Faculty Publications
The application of the field of network science to the scientific disciplines of structural biology and biochemistry, have yielded important new insights into the nature and determinants of protein structures, function, dynamics and the folding process. Advancements in further understanding protein relationships through network science have also reshaped the way we view the connectivity of proteins in the protein universe. The canonical hierarchical classification can now be visualized for example, as a protein fold continuum. This review will survey several key advances in the expanding area of research being conducted to study protein structures and folding using network approaches.
