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Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang 2023 Michigan Technological University

Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang

Michigan Tech Publications, Part 2

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They are typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these methods have made many projects in areas such as synthetic biology and protein engineering possible, they have various drawbacks. For example, they cannot produce genes and genomes with long repeats and have difficulty to produce sequences containing stable secondary structures. Here, we report a direct de novo chemical synthesis of 400 nt ODNs, and their isolation from the complex …


Smokejumper Obituary: Simons, Charles Richard (Missoula 1952), National Smokejumper Association 2023 Eastern Washington University

Smokejumper Obituary: Simons, Charles Richard (Missoula 1952), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.


Reclaiming Sacred Homelands: Asserting Treaty Rights And The Path Towards Restoration Of The Badger-Two Medicine, Sarah Greenberg 2023 Tribal Prosecutors Office, Confederated Salish & Kootenai Tribes

Reclaiming Sacred Homelands: Asserting Treaty Rights And The Path Towards Restoration Of The Badger-Two Medicine, Sarah Greenberg

American Indian Law Journal

“In order for law to have an influence in the lives of ordinary people, it must have something to do with the emotional feelings of justice, it must speak to our basic humanity, and it must give us common sense directions as to what behavior and beliefs are right and wrong"


Developing Novel Food Packaging Products With High Barrier Properties, Enabled By Cnf, Nabanita Das 2023 University of Maine

Developing Novel Food Packaging Products With High Barrier Properties, Enabled By Cnf, Nabanita Das

Electronic Theses and Dissertations

In recent times, plastic has become a highly favored choice for packaging due to its exceptional microbial, damage, and water-resistant properties. However, the alarming rise in plastic usage has led to adverse environmental pollution. This study aims to develop innovative food packaging solutions using renewable and compostable cellulose nanofibrils (CNFs). This thesis comprises two related studies on the barrier performance of novel food packaging materials, including oxygen barrier and oil/grease barrier properties. The goal of the first study was to enhance the mechanical and barrier properties of the cellulose nanofibril (CNF) films by inducing fibrils orientation for food packaging applications. …


Regeneration Response To Salvage Logging Following Tornado Disturbance, Colby K. Bosley-Smith 2023 University of Maine

Regeneration Response To Salvage Logging Following Tornado Disturbance, Colby K. Bosley-Smith

Electronic Theses and Dissertations

In an era of increasing natural disturbances, successful tree regeneration has grown more difficult to achieve. Salvage logging, a common management response to disturbance, may further impede regeneration success, although published literature currently remains inconclusive. In 2013, a rare tornado in northcentral Maine, USA, and subsequent salvage operation created three clear ‘treatments’ for evaluation of post-disturbance regeneration: blowdown, blowdown followed by salvage logging and an undisturbed control. In the summers of 2022 and 2023, (nine and ten) years post-tornado, we revisited this site to examine regeneration outcomes.

During the summer of 2022, we evaluated stand structure and regeneration success of …


Integrating Remote Sensing And Machine Learning To Assess Forest Health And Susceptibility To Pest-Induced Damage, Rajeev Bhattarai 2023 University of Maine

Integrating Remote Sensing And Machine Learning To Assess Forest Health And Susceptibility To Pest-Induced Damage, Rajeev Bhattarai

Electronic Theses and Dissertations

Spruce budworm (Choristoneura fumiferana; SBW) outbreaks are cyclically occurring phenomena in the northeastern USA and neighboring Canadian provinces. These outbreaks are often of landscape level causing impaired growth and mortality of the host species namely spruce (Picea sp.) and balsam fir (Abies balsamea (L.) Mill.). Acknowledging the recent SBW outbreak in Canadian provinces like Quebec and New Brunswick neighboring the state of Maine, our study devised comprehensive techniques to assess the susceptibility of Maine forests to SBW attack. This study aims to harness the power of remote sensing data and machine learning algorithms to model and map the susceptibility of …


Mobilizing Indigenous Research Methodologies And Wabanaki Knowledge In Biophysical Research To Restore Wabanaki Sweetgrass Harvesting In Acadia National Park And Identify Basket Quality Black Ash Habitat For Emerald Ash Borer (Agrilus Planipennis) Preparedness, Suzanne Greenlaw 2023 University of Maine

Mobilizing Indigenous Research Methodologies And Wabanaki Knowledge In Biophysical Research To Restore Wabanaki Sweetgrass Harvesting In Acadia National Park And Identify Basket Quality Black Ash Habitat For Emerald Ash Borer (Agrilus Planipennis) Preparedness, Suzanne Greenlaw

Electronic Theses and Dissertations

Black ash (wikpiyik/Fraxinus nigra) and sweetgrass (suwitokolasol/Anthoxanthum nitens) are two culturally important species to Wabanaki (Passamaquoddy, Penobscot, Maliseet, Mi’kmaq, and Abenaki) people, and to many other Native American tribes across the species’ ranges. Wabanaki relationships with black ash and sweetgrass include creation stories, generational stewardship practices, important economic markets, and cultural identity. Land use patterns, changes in access, invasive species, and climate change are negatively affecting both the health of black ash and sweetgrass and Wabanaki people’s relationship to these species. This dissertation consists of five chapters that mobilize Wabanaki knowledge and address Wabanaki access to sweetgrass and an invasive …


Forest Management In A Changing Climate: Integrating Social And Biopysical Sciences To Inform Adaptive Responses To Future Uncertainty, Peter Breigenzer 2023 University of Maine

Forest Management In A Changing Climate: Integrating Social And Biopysical Sciences To Inform Adaptive Responses To Future Uncertainty, Peter Breigenzer

Electronic Theses and Dissertations

Forests provide numerous ecological and socio-economic benefits, yet climate change is creating novel and extreme conditions that threaten forests and disrupt traditional management practices. To address future uncertainty about how to manage forests amid a rapidly changing climate, researchers have developed adaptive management strategies that move away from using historical ecological baselines as management goals. However, despite increases in adaptive forest management frameworks, there are still concerns that private woodland owners (PWOs; also known as family forest owners or non-industrial private landowners) are not adopting beneficial practices. Additionally, since tree canopies often buffer understory microclimates (i.e., fine scale variation in …


A Small Forest Owner's Engagement With A Carbon Sequestration Effort In Northeastern U.S., Frederick Pond 2023 University of Vermont

A Small Forest Owner's Engagement With A Carbon Sequestration Effort In Northeastern U.S., Frederick Pond

University Libraries Faculty and Staff Publications

In 2023, a small forest landowner in central Vermont enrolled 140 acres in the Family Forest Carbon Program[FFCP], engaging his local forestland in combating global warming.

FFCP is a collaboration of The Nature Conservancy and American Forest Foundation, developed to offer small landowners the opportunity to engage their asset in carbon sequestration locally.

This poster presents the experience of a small forest owner's process in entering a twenty year contract to manage a small woodlot under the direction of FFCP while enrolled with the state UVA program, also known as Current Use.

Challenges to the process, advantages/downsides, future perspectives are …


Smokejumper Obituary: Graves, Thomas Harry "Tom" (North Cascades 1949), National Smokejumper Association 2023 Eastern Washington University

Smokejumper Obituary: Graves, Thomas Harry "Tom" (North Cascades 1949), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.


Smokejumper Obituary: Sando, Rodney Wayne (Missoula 1965), National Smokejumper Association 2023 Eastern Washington University

Smokejumper Obituary: Sando, Rodney Wayne (Missoula 1965), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.


Evaluating Selected Properties Of Underutilized Hardwood Species For Fabrication Of Cross-Laminated Timber Industrial Mats, Mercy Itunu Ogunruku 2023 Mississippi State University

Evaluating Selected Properties Of Underutilized Hardwood Species For Fabrication Of Cross-Laminated Timber Industrial Mats, Mercy Itunu Ogunruku

Theses and Dissertations

Softwood is more in demand than hardwood because it is used primarily in the US's largest wood-consuming industry, construction, resulting in increased importation of softwood annually to meet this demand. Hardwood, used for non-structural purposes like furniture and interior designs, is more abundant in US forests. However, some hardwood species are underutilized and undervalued. Cross-laminated timber (CLT) has increased the demand for softwood.

A study evaluated the mechanical and physical properties of three underutilized hardwood species (321 yellow poplar, 393 sweetgum, and 262 red oak specimens) for CLT industrial mat manufacturing. The results showed that red oak had a higher …


Insights Into The Challenges Posed By Climate Change And Land Competition To Brazil’S Midwest Pulpwood Market, Roberto Hajime Sant Anna Kimura 2023 Mississippi State University

Insights Into The Challenges Posed By Climate Change And Land Competition To Brazil’S Midwest Pulpwood Market, Roberto Hajime Sant Anna Kimura

Theses and Dissertations

I investigated the effect of climate change in one of the few expanding markets in the world in Brazil. In the last decades the demand for pulpwood increased from zero to 11 million tons, leading to an expansion of 700 thousand hectares of Eucalyptus plantation. In 2024, a new mill will start operating increasing the consumption of wood fiber by 8.2 million tons (+49%). I used mathematical programming to investigate how different scenarios of productivity and land will affect the market. My results showed that around 946,000 hectares (+124%) of additional timberland will be necessary in the upcoming decades. The …


Barriers To Use Of Cross-Laminated Timber In Maine, Shane R. O'Neill 2023 University of Maine

Barriers To Use Of Cross-Laminated Timber In Maine, Shane R. O'Neill

Forest Resources Faculty Scholarship

To increase understanding of both the adoption rate and in-state manufacturing of mass timber In Maine, the 131st Legislature and Governor Mills passed LD 881, a resolve directing a study of the barriers facing cross-laminated timber In Maine and provide recommendations to promote their use in construction. This study was developed in response to the resolve. The study engaged 108 unique participants to define available training, education, and experiences across the stakeholders throughout the building lifecycle process in the state.

From this information, the following five recommendations are proposed:

  1. Understand the policies and initiatives of other states to develop …


Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. vonHoldt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John Vucetich, Jennifer A. Leonard, Robert K. Wayne 2023 Princeton University

Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John Vucetich, Jennifer A. Leonard, Robert K. Wayne

Michigan Tech Publications, Part 2

Effective population size estimates are critical information needed for evolutionary predictions and conservation decisions. This is particularly true for species with social factors that restrict access to breeding or experience repeated fluctuations in population size across generations. We investigated the genomic estimates of effective population size along with diversity, subdivision, and inbreeding from 162,109 minimally filtered and 81,595 statistically neutral and unlinked SNPs genotyped in 437 grey wolf samples from North America collected between 1986 and 2021. We found genetic structure across North America, represented by three distinct demographic histories of western, central, and eastern regions of the continent. Further, …


Deciding Sowing-Window For Maize-Based Cropping System In Arid And Semiaridenvironments In Punjab, Pakistan, MUKHTAR AHMED, ATIQUE REHMAN, NAEEM SARWAR, HÜLYA DOĞAN, GHULAM ABBAS, MUHAMMAD AZAM KHAN, MUSTAFA KAN, MUHAMMAD ALI RAZA, SAJJAD HUSSAIN, SHAKEEL AHMAD, ZARTASH FATIMA 2023 TÜBİTAK

Deciding Sowing-Window For Maize-Based Cropping System In Arid And Semiaridenvironments In Punjab, Pakistan, Mukhtar Ahmed, Atique Rehman, Naeem Sarwar, Hülya Doğan, Ghulam Abbas, Muhammad Azam Khan, Mustafa Kan, Muhammad Ali Raza, Sajjad Hussain, Shakeel Ahmad, Zartash Fatima

Turkish Journal of Agriculture and Forestry

Crop growth models can be valuable tools for researchers, academia, extension educators, and policy makers/planners for the evaluation of sustainable and long-term husbandry practices. Determining the optimum sowing window, which can be determined using crop growth models, is imperative under changing climate conditions. Thus, the main objectives herein were to 1) assess the performance of the cropping system model-crop environment resource synthesis-maize model for hybrids and sowing dates in the spring and autumn, and 2) determine the optimum sowing window in 15 districts of Punjab, Pakistan. In the spring experiment, 3 hybrids (P-33M15, M-DK6525, and S-NK8441) were planted in the …


Effects Of Genotype And Nitrogen Topdressing On Rapeseed Seed Yield And Stalkcombustion Properties, MARİNA BRČIĆ, TAJANA KRICKA, ANA POSPISIL, MILAN POSPISIL 2023 TÜBİTAK

Effects Of Genotype And Nitrogen Topdressing On Rapeseed Seed Yield And Stalkcombustion Properties, Mari̇na Brčić, Tajana Kricka, Ana Pospisil, Milan Pospisil

Turkish Journal of Agriculture and Forestry

The objective of this paper was to determine the effects of rapeseed genotype and nitrogen fertilization for dual purposes, i.e., on seed yield for human consumption and on the quantitative and qualitative value of harvested residues as feedstock to produce heat energy. The research was carried out in field trials set up under the agroecological conditions of northwestern Croatia at the University of Zagreb’s Faculty of Agriculture in the vegetation periods of 2012/2013 and 2013/2014. The research included three rapeseed genotypes (conventional hybrid SY Cassidy, cultivar Komando, and semidwarf hybrid PR45D03) and three nitrogen topdressing rates that included the supply …


Exploring The Diverse Applications Of Stigmasterol From Plants: A Comprehensive Review, UMMARA KHAN, FAISAL HAYAT, FAKHARA KHANUM, NAZİR AHMED, MOHAMED ABDİN, DYAAALDİN ABDALMEGEED, NEVA KARATAŞ, ZHİHONG XİN 2023 TÜBİTAK

Exploring The Diverse Applications Of Stigmasterol From Plants: A Comprehensive Review, Ummara Khan, Faisal Hayat, Fakhara Khanum, Nazi̇r Ahmed, Mohamed Abdi̇n, Dyaaaldi̇n Abdalmegeed, Neva Karataş, Zhi̇hong Xi̇n

Turkish Journal of Agriculture and Forestry

Stigmasterol has been studied for its numerous biological effects on various metabolic diseases, but its potential role as a ubiquitous biomolecule in the plant kingdom is still in its infancy. Efficient utilization of stigmasterol’s properties, such as antimicrobial, antioxidant, and self-assembly, is necessary for the food industry to effectively prevent microbial growth in food., in addition, to delaying rancidity by slowing down lipid oxidation. The use of safer alternatives for postharvest disease control, which is pressuring scientists to consider stigmasterol as a potential solution for various food-related issues. This article reviews stigmasterol's functions in the food industry, including its detection, …


Suppression Of Meloidogyne Incognita Race 2 With Some Entomopathogenic Agents, AYŞENUR ASLAN, CENK KESKİN, FATİH DADAŞOĞLU, ELİF TOZLU, ÖNDER ÇALMAŞUR, DİLEK DİNÇER, BEHÇET KEMAL ÇAĞLAR, İBRAHİM HALİL ELEKCİOĞLU 2023 TÜBİTAK

Suppression Of Meloidogyne Incognita Race 2 With Some Entomopathogenic Agents, Ayşenur Aslan, Cenk Keski̇n, Fati̇h Dadaşoğlu, Eli̇f Tozlu, Önder Çalmaşur, Di̇lek Di̇nçer, Behçet Kemal Çağlar, İbrahi̇m Hali̇l Elekci̇oğlu

Turkish Journal of Agriculture and Forestry

This study was conducted to investigate the effects of anaerobically stabilized sewage sludge (SS) converted into 90% dryness granules at different doses of control, 3% SS, 6% SS, and 9% SS on elemental composition of Festuca arundinacea Schreb. and physicochemical properties of soil. Color measurements were made with the CIE L* a* b* method unit; the highest color (4.10) and the highest coating score (5.7) were calculated for the 6% SS treatment. The highest biomass (9.11 g) was obtained from the 3% SS and the lowest (7.67 g) from the 9% SS dose. Plant height measurements were listed as follows: …


Some Physicochemical And Bioactive Features Of Organically Grown Blackberry Fruits(Rubus Fructicosus L.) As Influenced By Postharvest Uv-C And Chitosan Treatments, SEVİL ÜNAL, FERHAN KÜÇÜKBASMACI SABIR, ALİ SABIR 2023 TÜBİTAK

Some Physicochemical And Bioactive Features Of Organically Grown Blackberry Fruits(Rubus Fructicosus L.) As Influenced By Postharvest Uv-C And Chitosan Treatments, Sevi̇l Ünal, Ferhan Küçükbasmaci Sabir, Ali̇ Sabir

Turkish Journal of Agriculture and Forestry

Fresh blackberry fruits have easily perishable tissue and therefore have a very short postharvest storage life, although customers and marketers desire a longer shelf life. The ever-increasing demand of the world population for residue-free functional foods has motivated scientists to investigate environmentally friendly methodologies for the quality extension of perishable products like fresh blackberries. The present study was performed to determine the influences of UV-C treatment and chitosan coating on the extension of postharvest bioactive compounds and the marketable quality of fresh blackberry (Rubus fructicosus L.) fruits. Organically produced ‘Jumbo’ blackberry fruits were sorted into four equal groups for postharvest …


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