Food Science Commons^™

Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring

STAR Program Research Presentations

Characterization of Bacillus bacterial strains is an important step in identifying and removing biofilms contaminating processing equipment. These biofilms lead to large amounts of spores in the final product. Strains that produce spoilage enzymes cause off-flavors, structural defects and reduced shelf life of milk products. Using four treatments, we determined the best growth protocol for each strain to use in future experiments. Treatment 1: 80 ^o C heat shock and 32 ^o C 24hr incubation, Treatment 2: 80^o C/ 55 ^o C, Treatment 3: 100 ^o C/ 32 ^o C, Treatment 4: 100 ^o C/ 55 ^o C. Our …

Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli

Masters Theses & Specialist Projects

Scientific evidence shows that dietary intake of the omega-3 polyunsaturated fatty acids is beneficial to human health. Fish oil is a rich source of omega-3 fatty acids. However, fish oil with high levels of omega-3 PUFA is very susceptible to oxidative deterioration during storage. The objective of this study was to investigate the effect of xanthan gum (XG)-locust bean gum (LBG) mixtures on the physicochemical properties of whey protein isolate (WPI) stabilized oil-in-water (O/W) emulsions containing 20% v/v menhaden oil. The O/W emulsions containing XG/LBG mixtures were compared to emulsions with either XG or LBG alone. The emulsions were prepared …

Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones

Masters Theses & Specialist Projects

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. …

Development Of Hplc Methods For The Determination Of Water-Soluble Vitamins In Pharmaceuticals And Fortified Food Products, Hung Khiem Trang

All Theses

Though many HPLC methods have been developed and reported in literature for vitamin analysis for the past two decades, applying certain methods directly from literature more than often fails to reproduce the results reported due to many variables of liquid chromatography. This issue was targeted in this project through the examination of chromatographic behaviors of water-soluble vitamins in order to help the analysts better modify methods from literature or even develop new methods from scratch to fit their analytical need with the resources available (e.g., columns, detectors, etc.) in their lab.
The first part of the project investigated the chromatographic …

Kinetic Studies For The Preparation Of Probiotic Cabbage Juice: Impact On Phytochemicals And Bioactivity, Amit Jaiswal, Nissreen Abu-Ghannam

Articles

A kinetic study for the production of probiotic cabbage juice was carried out under controlled pH and dissolved oxygen using several strains of Lactic acid bacteria (LAB). Furthermore, effect of probiotic fermentation on polyphenolic content and antioxidant capacity was investigated. Results showed significant growth in Lactobacillus brevis, Lactobacillus plantarum and Lactobacillus rhamnosus corresponding to 9.19, 9.47 and 10.6 log CFU/ml after 24 h of fermentation, which is satisfying criteria for a food product to be characterized as probiotic. Lactic acid (LA) was the major end product of the fermented cabbage juice attaining the concentrations of 6.97, 9.69 and 12.2 g/l …

Excretion Of Phenolic Compounds After Consumption Of Fresh Versus Aged Chokeberry Juice In Rats, Sarah Elizabeth Graves

Graduate Theses and Dissertations

Flavonoids, such as anthocyanins, may be responsible for the health benefits of chokeberries, as they are present in large quantities in chokeberries and are potent antioxidant and anti-inflammatory agents. Despite these health properties, previous investigations on the bioavailability of flavonoids illustrate that little of the ingested amount reaches the bloodstream. This study investigated two factors that may help to explain these inconsistencies. First, storage of berry fruits and berry fruit products leads to extensive polymerization of their anthocyanins with procyanidins. Additionally, after consumption flavonoids may undergo metabolism into phenolic acids and subsequent conjugation to an extent that may explain their …

Influences Of Music Genre And Components On Food Perception And Acceptance, Alexandra Jean Fiegel

Graduate Theses and Dissertations

Managers of consumer goods companies (i.e., restaurants, grocery stores, and bars) have the potential to effectively utilize environmental factors to stimulate desired consumer behaviors. Background music has been identified as one of the most readily manipulated and influential elements to which a shopper or consumer may be exposed to in a service setting. Nevertheless, little is known about the effect of background sound on food perception and acceptance. This research sought whether background music genre and musical components can alter food perception and acceptance, but also to determine how the effect can vary as a function of food type (i.e., …

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pecan Residues In Processed Foods, Denise Tran

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Pecan nuts are becoming increasingly popular due to their link to health benefits. However, the presence of undeclared pecan residues in food products can pose serious health risks for pecan-allergic consumers. Currently, analytical methods for the detection of pecan allergens are limited and have not been validated for use by the food industry to assess the effectiveness of allergen control programs. Therefore, the aim of this study was to develop a sandwich-type ELISA to detect and quantify allergenic pecan residues in processed foods. Several varieties of pecan were mixed, roasted, and ground to immunize a goat, sheep, and rabbits. The …

Antibacterial Effects Of Proteases On Different Strains Of Escherichia Coli And Listeria Monocytogenes, Hanan Eshamah

All Dissertations

Escherichia coli O157:H7 and Listeria monocytogenes are pathogens that have received special attention by federal agencies, food safety researchers and food industries due to their economic and human health impact. To reduce the presence of these pathogens, alternative interventions have been studied. However, increasing consumer's demand for natural ingredients has made the investigations of effectiveness of natural antimicrobials necessary. In this study, in vitro antimicrobial activity of bromelain and papain against E. coli JM109 and L. monocytogenes was investigated. Furthermore, actinidin and papain were evaluated to reduce populations of L. monocytogenes strain and three mixed strains of E. coli O157:H7 …

An Evaluation Of The Nontarget Effects Of Transgenic Bacillus Thuringiensis Maize On Arbuscular Mycorrhizal Fungi In The Soil Ecosystem, Tanya Elizabeth Amy Cheeke

Dissertations and Theses

My dissertation research examined the effect of the cultivation of insect-resistant Bacillus thuringiensis (Bt) maize on the soil environment with a goal of understanding how to obtain a balance between technological advancement and maintenance of a healthy soil ecosystem. Although Bt plants may help to reduce pesticide use, conferring benefits to farm workers and the environment, there are still unresolved questions about how the cultivation of Bt plants affects soil organisms. For this dissertation project, I used 14 different genotypes of Bt maize and non-Bt maize (Zea mays) to investigate the effects of transgenic Bt plants on the colonization ability, …

Mutational Analysis Of Sse1 (Hsp110) Suggests An Integral Role For This Chaperone In Yeast Prion Propagation In Vivo, Ciara Moran, Gemma Kinsella, Zai-Rong Zhang, Sarah Perrett, Gary Jones

Articles

ABSTRACT The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily. Sse1 influences the cellular response to heat stress and has also been implicated in playing a role in the propagation of prions in yeast. Sse1 can seemingly exert its effects in vivo through direct or indirect actions by influencing the nucleotide exchange activity of canonical cytosolic Hsp70s. Using a genetic screen based on the inability to propagate the yeast [PSI+] prion, we have identified 13 new Sse1 mutants that are predicted to alter chaperone function through a variety of different …

Development Of Rapid Detection Methods And Novel Control Measures For Salmonella In Poultry, Si Hong Park

Graduate Theses and Dissertations

This dissertation consists of three research parts: 1) development of rapid detection methods for foodborne pathogens; 2) immune response of chicken cells against Salmonella and bacteriophage P22; 3) evaluation of novel control measures for poultry productions. In order to develop rapid and accurate detection methods for foodborne pathogens, two types of PCR assays were utilized. Three foodborne pathogens included Campylobacter, Escherichia coli and Salmonella in watershed were qualitatively and quantitatively detected by multiplex PCR and qPCR (chapter 2). Since Salmonella species are commonly present in poultry and poultry products as well as most popular foodborne pathogen in …

The View From The Table: An Analysis Of Participant Reactions To Community-Based Dialogues On Food And Justice, Jennifer Turner

Dissertations and Theses

While Portland, Oregon's sustainable food movement wins accolades for explicitly situating itself in opposition to the industrialized global food system, it often fails to address systems of oppression that are reproduced within the alternative agri-food movement itself. This demonstrated aversion towards the messy, complex, contingent nature of the social world reflects larger processes of "de-politicization" of the overall sustainability agenda, which leads to the favoring of technological and/or spatial solutions that may undermine the social equity and justice dimensions of the "triple bottom line." This thesis focuses on an action research project involving a series of community dialogues that provided …

Fearless: Heather Thomas, Heather L. Thomas

SURGE

This week we would like to recognize recent graduate and AmeriCorps VISTA volunteer Heather Thomas ’13, who is returning to Gettysburg as the fearless new coordinator for the Adams County Food Policy Council. [excerpt]

Milking The System: Do Poor People Deserve Fresh Food?, Melanie M. Meisenheimer

SURGE

Poor Americans are all lazy, selfish people who must first prove their worth as human beings if they want to be able to feed their children.

It sounds harsh, stereotypical, and judgmental when you put it like that, and few people would feel comfortable saying that exact phrase. However, it’s a perception of poverty in America that I’ve found still has a strong grip on our way of thinking. [excerpt]

Storage Technique Preserves Citrus, Kills Pathogens, Gayle Volk, Jan Suszkiw

Agricultural Research Magazine

Americans consume about 84 pounds of fresh and processed citrus per person each year, with oranges topping the list at 61 pounds annually. The availability of these favored fruits in grocery stores, fresh markets, and other consumer outlets is a testament to growers’ success in managing the ever-present threat of pests and diseases to the nation’s $3.4 billion citrus crop (2011-12), most of which is produced in Florida and California.

A major threat is citrus greening disease, also known as “Huanglongbing.” First detected in Florida in August 2005, citrus greening today is the target of a multifaceted effort by federal, …

Detecting Deadly Colonies Of E. Coli, Rosalie Marion Bliss

Agricultural Research Magazine

Shiga-toxin-producing Escherichia coli (STEC) serogroup O157:H7 has long been associated with severe forms of foodborne illnesses. But these bacterial bad guys have many cousins, and six other STEC groups have also been linked to serious illnesses and outbreaks of disease.

In 2011, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced a regulation that, as of June 2012, certain beef products that test positive for any of these six groups, nicknamed the “Big Six,” would be banned from being sold for public consumption. As USDA’s regulatory arm, FSIS employs inspectors who collect meat samples on the front …

Current Challenges Can Help Fuel Future Opportunities, Jeffrey J. Steiner

Agricultural Research Magazine

Native juniper trees and invasive plants pose an expanding threat to the survival of the sagebrush ecosystem in arid western rangelands. As the trees mature, they compete with other native plants that are valuable food sources for wildlife and cattle. After these plants die back, the bare patches of soil that remain are vulnerable to erosion and encroachment from other species like cheatgrass, an invasive nonnative annual that fuels wildfires.

Wildfires threaten livestock ranches, are suppressed in large part by efforts funded with taxpayer dollars, and increase the probability that invasive plants will spread and thrive in the postfire landscape. …

Better French Fries: Technique Helps Lower Fat, Zhongli Pan, Marcia Wood

Agricultural Research Magazine

No doubt about it. Americans love French fries.

But fries soak up a lot of oil while they’re turning a perfect golden brown in the deep-fat fryer.

Agricultural Research Service scientist Zhongli Pan and colleagues have tackled the challenge of lowering some of the fat from this favorite side order without skimping on the fries’ delicious taste and texture or their pleasing appearance and aroma. Their idea? After potatoes are peeled and sliced into strips, and just before the raw strips are dunked in the fryer, prep them with 3 minutes of infrared (IR) heat—just like that created in home …

Fungi Can Be Friends Or Foes, Amy Rossman, Jo Anne Crouch, Shannon Dominick, Sharon Durham

Agricultural Research Magazine

Fungi are a large and diverse group of organisms. Some fungi, like mushrooms, are edible and considered crops. Others, however, can cause serious diseases of crop and forest plants, and those diseases can have negative effects on local and international economies as well as on the supply of food and other materials that agriculture provides.

The Agricultural Research Service maintains a unique resource—the U.S. National Fungus Collections—for helpful and detailed information about fungi. Accurate knowledge of fungi is critical for controlling the diseases they cause.

North America’s Largest Fungarium

In 1869, the Smithsonian Institution transferred its fungal collection to the …