Food Science Commons^™

The Role Of Underutilized Fish In New England's Seafood System, Taylor Witkin

Honors Theses

The global fisheries crisis has led to an increasing recognition for the need to relieve pressure on overfished, popular food fish stocks. Opportunities exist to shift consumer demand toward more sustainable choices, including fish that may be locally abundant. Attempts have been made to market underused fish (sometimes termed “trash fish”) that represent more sustainable alternatives; however, it is unclear whether consumers will choose to purchase these more sustainable options, particularly if underused fish are also unfamiliar. Chapter 1 reviews existing research and current issues surrounding sustainable seafood and the recent shift toward local, abundant, undervalued species in New England’s …

Isolation And Characterization Of Natural Antimicrobials From Plant And Marine Organisms, Corene Barbara Canning

Wayne State University Dissertations

Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial …

Characterization Of Bacteriophages From Environmental Water Samples And The Potential Of Bacteriophages Tailspike Proteins (Tsp) In Bacteria Detection, Gayathri Upeksha Gunathilaka

Wayne State University Theses

ABSTRACT

CHARACTERIZATION OF BACTERIOPHAGES FROM ENVIRONMENTAL WATER SAMPLES AND THE POTENTIAL OF BACTERIOPHAGES TAILSPIKE PROTEINS (TSP) IN BACTERIA DETECTION

by

GAYATHRI UPEKSHA GUNATHILAKA

May 2014

Advisor: Dr. Yifan Zhang

Major: Nutrition and Food Science

Degree: Master of Science

The high abundance of phage in the environment and their specificity with the bacteria host make them an excellent tool for bacteria detection. To characterize phage specific to Salmonella and E. coli from water samples in the Southeastern Michigan and to develop a novel bacterial detection method using phage, environmental water samples were collected from rivers and waste water treatment plant (WWTP) …

Rheological, Foam, And Physical Properties Of Low Sucrose Meringue And Angel Food Cake Formulated With Non-Nutritive Sweeteners And Polydextrose, Kevin J. O'Niones

Theses and Dissertations--Animal and Food Sciences

The object of this research was to determine if an acceptable angel food cake alternative could be produced that had reduced calories and sucrose content. This was accomplished through replacing sucrose in meringue, angel food cake batter, and baked angel food cakes with polydextrose and either sucralose, acesulfame-K, or Rebaudioside A at different replacement levels (25, 50, 75, 100%). Meringue and cake batter properties were measured using rheological techniques. Baked angel food cakes were analyzed based on height, weight loss, moisture content, color, and TPA analysis. With meringue batter, 100% sucrose replacement was unacceptable since undissolved polydextrose made analyzing and …

The Acoustic Emissions Produced By Escherichia Coli During The Growth Cycle, Traci Jane Cox

Theses and Dissertations--Animal and Food Sciences

The objective of this study was to determine if acoustic emissions (AE) generated by three strains of Escherichia Coli (5024-parent strain, 8279-mutant strain and 8279-random/unrelated strain) could be used to differentiate each strain during their growth cycle. An acoustic sensor with an operating range of 35 kHz-100 kHz was inserted into the growth vessel and attached to a selected channel to capture AE data. The growth vessel was loaded with 60 ml of tryptic soy broth (TSB) (0.25% fructose) media with alginate (1.1%) or without alginate and inoculated with 1% (10⁸ CFU/ml) of an E. coli strain. The growth …

Herbal And Microbial Products For The Management Of Obesity, Maha Salama, Soheir El Zalabani, Essam Abdel Sattar

Pharmacy

Obesity is a global epidemic and one of the major health burdens of modern times. The prevalence of obesity is increasing worldwide; it constitutes a serious problem in developed as well as developing countries. Beside adults, the number of obese teenagers and children in particular has dramatically increased. Obesity is characterized by accumulation of excess fat in adipose tissues in an extent to produce adverse effects on health, leading to a reduction in life expectancy and/or a raise in health hazards. People are classified as overweight (pre-obese) and obese on the basis of the Body Mass Index (BMI), crude measure …

Modelling The Effects Of Natural Antimicrobials As Food Preservatives, Amit Jaiswal, Swarna Jaiswal

Books/Book Chapters/ Proceedings

A predictive food microbiological model is a mathematical representation of mechanisms that describes the growth, survival, inactivation or biochemical process of an organism and are used in the food industry to predict food pathogen growth and to help in the evaluation of food safety. Furthermore, it reduces the number of expensive and time-consuming experiments; however, adequate statistical analysis is a crucial step during all phases of model development and validation. Therefore, the present chapter is focused on the basic concept of mathematical modelling which can be applied to estimate the effects of natural antimicrobials as food preservatives. Initial section discusses …

Screening Of Natural Antimicrobial Agents And Antimicrobial-Resistant Bacteria Using A Soleris System, Mingyang Huang

Wayne State University Theses

ABSTRACT

SCREENING OF NATURAL ANTIMICROBIAL AGENTS AND ANTIMICROBIAL - RESISTANT BACTERIA USING A SOLERIS SYSTEM

by

MINGYANG HUANG

May 2014

Advisor: Dr. Yifan Zhang

Major: Food Science and Nutrition

Degree: Master of Science

Traditional broth micro-dilution method is a common assay of measuring Minimum Inhibitory Concentration (MIC) to determine the antimicrobial activity of an antimicrobial agent. However, this method is generally time-consuming and labor intensive. Alternatively, an automated optical method using the Soleris system was applied in this study. The system was compared to the traditional broth micro-dilution 96-well assay to test the antimicrobial activity of 11 essential oils and …

Antimicrobial Activity Of Essential Oil Emulsions And Possible Synergistic Effect On Food Borne Pathogens, Varun Tahlan

Wayne State University Theses

The objective of this study was to evaluate the antimicrobial activity of essential oil emulsions against food borne pathogenic bacteria and determine potential applications. The oils used for this study were cinnamon, oregano, clove, thyme, rosemary, sage, bergamot, nutmeg, lemon grass and bay. Oil in water emulsions were prepared using Tween 80 as an emulsifying agent, with a stock oil concentration in the emulsions of 20,000 ppm. Essential oil emulsions were individually screened against E. coli (ATCC 25922), E. coli (ATCC 700927), L. monocytogens (ATCC 19115), L. innocua (ATCC 33090) and S. Typhimurium (ATCC 19585) using the broth micro dilution …

Bacteriophage Isolated From The Environment And E. Coli Of Meat Origin As A Reservoir Of Antibiotic Resistance, Manisha Polur

Wayne State University Theses

ABSTRACT

BACTERIOPHAGE ISOLATED FROM THE ENVIRONMENT AND E. COLI OF MEAT ORIGIN AS A RESERVOIR OF ANTIBIOTIC RESISTANCE

by

MANISHA POLUR

April 2014

Advisor: Dr. Yifan Zhang

Major: Nutrition and Food Science

Degree: Master of Science

This study was aimed to determine the extent of antibiotic resistance reservoir in bacteriophage. Bacteriophages were isolated and purified from waste water treatment plants, Detroit river, Belle Isle, Urban Garden soils and examined for the presence of beta-lactam antibiotic resistance genes. Thirty E. coli isolates from retail meat were analyzed for the presence of beta-lactam antibiotic resistance genes. 95% of E. coli them carried …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

College Students’ Perceptions Regarding Sensory Aspects Of Conventionally Produced And Unconventionally Produced Foods: Implications For Marketing To The Millennial Generation, Christina Crowder, Catherine W. Shoulders, K. Jill Rucker

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Consumers vote every day on which products line the shelves of grocery stores, co-ops, and niche markets. Public unrest with regard to the environmental, animal welfare, food purity, and human health impacts of agricultural production practices have led to the rise of unconventionally produced (UP) food products. While the sales of UP foods is increasing, studies regarding the qualities of such products that impact consumer purchases have yielded inconsistent results. This study examined students’ perceptions of sensory aspects of conventionally produced (CP) and UP foods to better understand how sensory aspects impact decisions to purchase. Students reported consistent perceptions regarding …

Effectiveness Of A Farm Field Trip, Bonnie S. Sigmon

Theses and Dissertations--Community & Leadership Development

The annual Sigmon Farm Tour was started in 1992 as an agricultural education program where students could experience being on a farm with the goal of increasing the agricultural literacy levels of the participants. Every year the entire 4th grade student population of Rockcastle County spends the day touring the farm and participating in experiential mini lessons given by the cooperating farm service and health agencies. The program has continued for 20+ years without an evaluation as to whether it is achieving its objectives. This evaluation will also exhibit the programs strengths and weakness so it can continue to improve. …

Ultrasound Assisted Hydration Of Navy Beans (Phaseolus Vulgaris), N. Misra

Articles

The use of ultrasound to enhance the transport phenomena in food processes has been well recognised in recent times. The objective of this study was to evaluate the effect of sonication on hydration rate and pasting profile of navy beans. The hydration kinetics for control and ultrasound assisted soaking was mathematically described using mechanistic (Fickian diffusion) and empirical (Peleg’s equation, Weibull model and First Order equation) models. Ultrasound enhanced the rate of hydration which was evident from the plot of kinetic data and model parameters. The effective diffusivities for water transport without and with ultrasound application were estimated to be …

Improved Efficiency Of Brewer’S Spent Grain Arabinoxylans By Ultrasound-Assisted Extraction, Sofia Reis, Elisabete Coelho, Manuel Coimbra, Nissreen Abu-Ghannam

Articles

Arabinoxylan (AX) rich extracts from brewer’s spent grain (BSG) were produced by the application of ultrasound-assisted extraction (UAE) and conventional alkaline extraction (AKE). UAE and AKE were optimised for the production of the highest yield of ethanol insoluble material using response surface methodology (RSM). The efficiency of UAE was established by the significant reduction of time (7h to 25 min) and energy when compared to AKE, to recover similar amount of AX (60%) from BSG, leading to the production of starch-free AX-rich extracts.

Development Of A Novel Functional Soup Rich In Bioactive Sulforaphane Using Broccoli (Brassica Oleracea L. Ssp. Italica) Florets And Byproducts., Laura Alvarez Jubete, Juan Valverde, Kate Kehoe, Kim Reilly, Dilip K. Rai, Catherine Barry-Ryan

Articles

Broccoli florets are rich in health-promoting compounds such as glucoraphanin, the precursor of the bioactive compound sulforaphane. In addition, broccoli byproducts such as broccoli stalk also contain health promoting compounds and represent attractive ingredients in the development of functional foods. The bioconversion of glucosinolates into bioactive isothiocyanates is affected by many factors including heat and therefore cooking of Brassicas such as broccoli may result in significant loss of sulforaphane production. The aim of this study was to develop a suitable food system as a vehicle for the delivery of sulforaphane in the human diet in adequate quantities. To this end, …

Atmospheric Cold Plasma Inactivation Of Escherichia Coli, Salmonella Enterica Serovar Typhimurium And Listeria Monocytogenes Inoculated On Fresh Produce, Dana Ziuzina, Sonal Patil, Patrick J. Cullen, Kevin Keener, Paula Bourke

Articles

Atmospheric cold plasma (ACP) represents a potential alternative to traditional methods for non-thermal decontamination of foods. In this study, the antimicrobial efficacy of a novel dielectric barrier discharge ACP device against Escherichia coli, Salmonella enterica Typhimurium and Listeria monocytogenes inoculated on cherry tomatoes and strawberries, was examined. Bacteria were spot inoculated on the produce surface, air dried and sealed inside a rigid polypropylene container. Samples were indirectly exposed (i.e. placed outside plasma discharge) to a high voltage (70kVRMS) air ACP and subsequently stored at room temperature for 24 h. ACP treatment for 10, 60 and 120 s resulted in reduction …

Zein Film: Effects Of Dielectric Barrier Discharge Atmospheric Cold Plasma, Shashi Pankaj, Carmen Bueno-Ferrer, N. Misra, Paula Bourke, Patrick J. Cullen

Articles

Dielectric barrier discharge atmospheric plasma is a novel non-thermal technology for the food and packaging industry. The effects of dielectric barrier discharge plasma on the surface, structural, thermal and moisture sorption properties of edible zein films have been examined. Plasma treatment increased the surface roughness and equillibrium moisture content of the zein film in a direct relationship with the applied voltage level. No significant difference in the thermal stability of the zein film is also observed after plasma treatment. Dielectric barrier discharge plasma treatments of zein film lead to a change in the protein conformation which is confirmed by X-ray …

Apple Pomace As A Potential Ingredient For The Development Of New Functional Foods, Sofia Reis, Dilip K. Rai, Nissreen Abu-Ghannam

Articles

Extruded snacks and baked scones were formulated with increasing levels (0-30%) of apple pomace (AP). The incorporation of up to 20% of AP in extruded snacks and in baked scones does not change significantly (pβ-carotene/linoleic acid system) increased when compared to the products to which no AP was added. Chlorogenic acid and quercetin were the major phenolic compounds found in the products. Loss of phenolic compounds during heat treatment occurred, however the DPPH radical scavenging activity of final products was not affected.

Microbial Successions Are Associated With Changes In Chemical Profiles Of A Model Refrigerated Fresh Pork Sausage During An 80-Day Shelf Life Study, Andrew K. Benson, Jalrus R.D. David, Stefanie Evans Gilbreth, Gordon Smith, Joseph Nietfeldt, Ryan Legge, Jaehyoung Kim, Rohita Sinha, Christopher E. Duncan, Junjie Ma, Indarpal Singh

Department of Food Science and Technology: Faculty Publications

Fresh pork sausage is produced without a microbial kill step and therefore chilled or frozen to control microbial growth. In this report, the microbiota in a chilled fresh pork sausage model produced with or without an antimicrobial combination of sodium lactate and sodium diacetate was studied using a combination of traditional microbiological methods and deep pyrosequencing of 16S rRNA gene amplicons. In the untreated system, microbial populations rose from 10² to 10⁶ CFU/g within 15 days of storage at 4°C, peaking at nearly 10⁸ CFU/g by day 30. Pyrosequencing revealed a complex community at day 0, with …