Food Science Commons^™

Ultrasound Assisted Hydration Of Navy Beans (Phaseolus Vulgaris), N. Misra

Articles

The use of ultrasound to enhance the transport phenomena in food processes has been well recognised in recent times. The objective of this study was to evaluate the effect of sonication on hydration rate and pasting profile of navy beans. The hydration kinetics for control and ultrasound assisted soaking was mathematically described using mechanistic (Fickian diffusion) and empirical (Peleg’s equation, Weibull model and First Order equation) models. Ultrasound enhanced the rate of hydration which was evident from the plot of kinetic data and model parameters. The effective diffusivities for water transport without and with ultrasound application were estimated to be …

Apple Pomace As A Potential Ingredient For The Development Of New Functional Foods, Sofia Reis, Dilip K. Rai, Nissreen Abu-Ghannam

Articles

Extruded snacks and baked scones were formulated with increasing levels (0-30%) of apple pomace (AP). The incorporation of up to 20% of AP in extruded snacks and in baked scones does not change significantly (pβ-carotene/linoleic acid system) increased when compared to the products to which no AP was added. Chlorogenic acid and quercetin were the major phenolic compounds found in the products. Loss of phenolic compounds during heat treatment occurred, however the DPPH radical scavenging activity of final products was not affected.

Atmospheric Cold Plasma Inactivation Of Escherichia Coli, Salmonella Enterica Serovar Typhimurium And Listeria Monocytogenes Inoculated On Fresh Produce, Dana Ziuzina, Sonal Patil, Patrick J. Cullen, Kevin Keener, Paula Bourke

Articles

Atmospheric cold plasma (ACP) represents a potential alternative to traditional methods for non-thermal decontamination of foods. In this study, the antimicrobial efficacy of a novel dielectric barrier discharge ACP device against Escherichia coli, Salmonella enterica Typhimurium and Listeria monocytogenes inoculated on cherry tomatoes and strawberries, was examined. Bacteria were spot inoculated on the produce surface, air dried and sealed inside a rigid polypropylene container. Samples were indirectly exposed (i.e. placed outside plasma discharge) to a high voltage (70kVRMS) air ACP and subsequently stored at room temperature for 24 h. ACP treatment for 10, 60 and 120 s resulted in reduction …

Zein Film: Effects Of Dielectric Barrier Discharge Atmospheric Cold Plasma, Shashi Pankaj, Carmen Bueno-Ferrer, N. Misra, Paula Bourke, Patrick J. Cullen

Articles

Dielectric barrier discharge atmospheric plasma is a novel non-thermal technology for the food and packaging industry. The effects of dielectric barrier discharge plasma on the surface, structural, thermal and moisture sorption properties of edible zein films have been examined. Plasma treatment increased the surface roughness and equillibrium moisture content of the zein film in a direct relationship with the applied voltage level. No significant difference in the thermal stability of the zein film is also observed after plasma treatment. Dielectric barrier discharge plasma treatments of zein film lead to a change in the protein conformation which is confirmed by X-ray …

Improved Efficiency Of Brewer’S Spent Grain Arabinoxylans By Ultrasound-Assisted Extraction, Sofia Reis, Elisabete Coelho, Manuel Coimbra, Nissreen Abu-Ghannam

Articles

Arabinoxylan (AX) rich extracts from brewer’s spent grain (BSG) were produced by the application of ultrasound-assisted extraction (UAE) and conventional alkaline extraction (AKE). UAE and AKE were optimised for the production of the highest yield of ethanol insoluble material using response surface methodology (RSM). The efficiency of UAE was established by the significant reduction of time (7h to 25 min) and energy when compared to AKE, to recover similar amount of AX (60%) from BSG, leading to the production of starch-free AX-rich extracts.

Rheological, Foam, And Physical Properties Of Low Sucrose Meringue And Angel Food Cake Formulated With Non-Nutritive Sweeteners And Polydextrose, Kevin J. O'Niones

Theses and Dissertations--Animal and Food Sciences

The object of this research was to determine if an acceptable angel food cake alternative could be produced that had reduced calories and sucrose content. This was accomplished through replacing sucrose in meringue, angel food cake batter, and baked angel food cakes with polydextrose and either sucralose, acesulfame-K, or Rebaudioside A at different replacement levels (25, 50, 75, 100%). Meringue and cake batter properties were measured using rheological techniques. Baked angel food cakes were analyzed based on height, weight loss, moisture content, color, and TPA analysis. With meringue batter, 100% sucrose replacement was unacceptable since undissolved polydextrose made analyzing and …

The Acoustic Emissions Produced By Escherichia Coli During The Growth Cycle, Traci Jane Cox

Theses and Dissertations--Animal and Food Sciences

The objective of this study was to determine if acoustic emissions (AE) generated by three strains of Escherichia Coli (5024-parent strain, 8279-mutant strain and 8279-random/unrelated strain) could be used to differentiate each strain during their growth cycle. An acoustic sensor with an operating range of 35 kHz-100 kHz was inserted into the growth vessel and attached to a selected channel to capture AE data. The growth vessel was loaded with 60 ml of tryptic soy broth (TSB) (0.25% fructose) media with alginate (1.1%) or without alginate and inoculated with 1% (10⁸ CFU/ml) of an E. coli strain. The growth …

Microbial Successions Are Associated With Changes In Chemical Profiles Of A Model Refrigerated Fresh Pork Sausage During An 80-Day Shelf Life Study, Andrew K. Benson, Jalrus R.D. David, Stefanie Evans Gilbreth, Gordon Smith, Joseph Nietfeldt, Ryan Legge, Jaehyoung Kim, Rohita Sinha, Christopher E. Duncan, Junjie Ma, Indarpal Singh

Department of Food Science and Technology: Faculty Publications

Fresh pork sausage is produced without a microbial kill step and therefore chilled or frozen to control microbial growth. In this report, the microbiota in a chilled fresh pork sausage model produced with or without an antimicrobial combination of sodium lactate and sodium diacetate was studied using a combination of traditional microbiological methods and deep pyrosequencing of 16S rRNA gene amplicons. In the untreated system, microbial populations rose from 10² to 10⁶ CFU/g within 15 days of storage at 4°C, peaking at nearly 10⁸ CFU/g by day 30. Pyrosequencing revealed a complex community at day 0, with …

Criteria Used To Categorise Proteins As Allergens For Inclusion In Allergenonline.Org: A Curated Database For Risk Assessment, Richard E. Goodman, Ronald Van Ree, Stefan Vieths, Fatima Ferreira, Motohiro Ebisawa, Hugh A. Sampson, Joseph Baumert, John Wise, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

New proteins introduced in foods through genetic engineering or processing are often evaluated for potential risks of eliciting food allergy (Codex, 2003). The primary risk is the transfer of an allergen or a protein nearly identical to an allergen and capable of causing IgE mediated cross-reactions into a new food source. The AllergenOnline. org database was developed to provide a curated set of allergenic sequences for bioinformatics comparisons to identify proteins that should be tested further (e.g. serum IgE binding). Curation is necessary as the scientific literature and general sequence databases include thousands of proteins labeled as allergens that lack …

Salmonella Enterica Serovar Typhimurium-Infected Pigs With Different Shedding Levels Exhibit Distinct Clinical, Peripheral Cytokine And Transcriptomic Immune Response Phenotypes, Susan Knetter, Shawn Bearson, Ting-Hua Huang, Dason Kurkiewicz, Martine Schroyen, Dan Nettleton, Daniel Berman, Valorie Cohen, Joan Lunney, Amanda Ramer-Tait, Michael Wannemuehler, Christopher Tuggle

Department of Food Science and Technology: Faculty Publications

Foodborne salmonellosis costs the US $2.7 billion/year, including $100.0 million in annual losses to pork producers. Pigs colonized with Salmonella are usually asymptomatic with varied severity and duration of fecal shedding. Thus, understanding the responses that result in less shedding may provide a mechanism for control. Fifty-four pigs were inoculated with Salmonella enterica serovar Typhimurium (ST) and clinical signs, fecal ST shedding, growth performance, peripheral cytokines and whole blood gene expression were measured. Persistently shedding (PS) pigs had longer pyrexia and elevated serum IL-1β, TNF-α and IFN-ϒcompared with low shedding (LS) pigs, while LS pigs had brief pyrexia, less shedding …

In Silico And Ex Vivo Approaches Identify A Role For Toll-Like Receptor 4 In Colorectal Cancer, Daniel A. Sussman, Rebeca Santoalalla, Pablo A. Bejarano, Monica T. Garcia-Buitrago, Maria T. Perez, Maria T. Abreu, Jennifer L. Clarke

Department of Food Science and Technology: Faculty Publications

Background: Inflammation increases the risk of colorectal cancer (CRC). We and others have described a role for TLR4, the receptor for LPS, in colon cancer. To explore the relationships between TLR4 expression and CRC, we combined the strength of transcriptome array data and immunohistochemical (IHC) staining. Methods: TLR4 signal intensity was scored in the stromal and epithelial compartments. Detection of differential expression between conditions of interest was performed using linear models, Cox proportional hazards models, and empirical Bayes methods. Results: A strong association between TLR4 expression and survival was noted, though a dichotomous relationship between survival and specific TLR4 transcripts …

Update Of The Who/Iuis Allergen Nomenclature Database Based On Analysis Of Allergen Sequences, C. Radauer, A. Nandy, F. Ferreira, Richard E. Goodman, J. N. Larsen, J. Lidholm, A. Pomés, M. Raulf-Heimsoth, P. Rozynek, W. R. Thomas, Heimo Breiteneder

Department of Food Science and Technology: Faculty Publications

The IUIS Allergen Nomenclature Sub-Committee, under the auspices of the World Health Organization and the International Union of Immunological Societies, maintains the systematic nomenclature of allergenic proteins and publishes a database of approved allergen names on its Web site, www.allergen.org. In this paper, we summarize updates of allergen names approved at the meetings of the committee in 2011 through 2013. These changes reflect recent progress in identification, cloning, and sequencing of allergens. The goals of this update were to increase consistency in the classification of allergens, isoallergens, and variants and in the incorporation of the evolutionary classification of proteins into …

Safety Assessment Of The Calcium-Binding Protein, Apoaequorin, Expressed By Escherichia Coli, Daniel L. Moran, Afua O. Tetteh, Richard E. Goodman, Mark Y. Underwood

Department of Food Science and Technology: Faculty Publications

Calcium-binding proteins are ubiquitous modulators of cellular activity and function. Cells possess numerous calcium-binding proteins that regulate calcium concentration in the cytosol by buffering excess free calcium ion. Disturbances in intracellular calcium homeostasis are at the heart of many age-related conditions making these proteins targets for therapeutic intervention. A calcium-binding protein, apoaequorin, has shown potential utility in a broad spectrum of applications for human health and well-being. Large-scale recombinant production of the protein has been successful; enabling further research and development and commercialization efforts. Previous work reported a 90-day subchronic toxicity test that demonstrated this protein has no toxicity by …

Grain Sorghum Whole Kernel Oil Lowers Plasma And Liver Cholesterol In Male Hamsters With Minimal Wax Involvement, Bo Hyun Lee, Thomas M. Carr, Curtis Weller, Susan L. Cuppett, Ismail M. Dweikat, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

The lipid fraction of the grain sorghum whole kernel (GS-WK) (i.e., phytosterol rich oil or policosanol rich wax) responsible for lowering cholesterol in hamsters fed the crude lipid (wax + oil) was determined. As expected, hamsters fed an atherogenic diet for a four week period presented with higher plasma non-HDL plasma and liver esterified cholesterol than those on the low fat diet. However, the atherogenic diet containing 5% (w/w) oil significantly lowered non-HDL plasma and liver cholesterol. Although the 5% wax supplement did not affect either plasma or liver cholesterol, excreted neutral sterol and bile acid were slightly higher than …

Investigation Of Bacterial Diversity In The Feces Of Cattle Fed Different Diets, M. Kim, J. Kim, L. A. Kuehn, J. L. Bono, E. D. Berry, N. Kalchayanand, Harvey C. Freetly, Andrew K. Benson, J. E. Wells

Department of Food Science and Technology: Faculty Publications

The objective of this study is to investigate individual animal variation of bovine fecal microbiota including as affected by diets. Fecal samples were collected from 426 cattle fed 1 of 3 diets typically fed to feedlot cattle: 1) 143 steers fed finishing diet (83% dry-rolled corn, 13% corn silage, and 4% supplement), 2) 147 steers fed late growing diet (66% dry-rolled corn, 26% corn silage, and 8% supplement), and 3) 136 heifers fed early growing diet (70% corn silage and 30% alfalfa haylage). Bacterial 16S rRNA gene amplicons were determined from individual fecal samples using next-generation pyrosequencing technology. A total …

Activation Of Autophagy And Ampk By Gamma-Tocotrienol Suppresses The Adipogenesis In Human Adipose Derived Stem Cells, Lu Zhao, Jung-Heun Ha, Meshail Okla, Soonkyu Chung

Department of Food Science and Technology: Faculty Publications

Scope: This study investigated the mechanistic details by which gamma-tocotrienol (γ-T3) manipulates adipocyte differentiation in human adipose derived stem cells (hASCs).

Methods and results: γ-T3 specifically inhibited the early stage of adipocyte differentiation by acting on downstream of C/EBP-β but upstream of C/EBP-α in hASCs. In searching a potential mechanism, we identified that γ-T3 promoted two catabolic signaling pathways: (i) AMP kinase (AMPK), and (ii) enhanced autophagy, as assessed by autophagic flux and cytosolic autophagosome (LC3II) accumulation. In addition, chronic exposure of γ-T3 induced caspase3-mediated apoptotic cell death. The blockage of AMPK by a dominant negative mutant of …

Structure Of Clostridium Difficile Pilj Exhibits Unprecedented Divergence From Known Type Iv Pilins, Kurt H. Piepenbrink, Grace A. Maldarelli, Claudia F. Martinez De La Pena, George L. Mulvey, Greg A. Snyder, Leon De Masi, Erik C. Von Rosenvinge, Sebastian Gunther, Glen D. Armstrong, Michael S. Donnenberg, Eric J. Sundberg

Department of Food Science and Technology: Faculty Publications

Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, cellular adhesion, and colonization. Recently, there has been an increased appreciation of the ability of Gram-positive species, including Clostridium difficile, to produce Type IV pili. Here we report the first three-dimensional structure of a Gram-positive Type IV pilin, PilJ, demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C. difficile may assemble and function. PilJ has several unique structural features, including a dual-pilin fold and the incorporation of a structural …

Epidemic Clostridium Difficile Strains Demonstrate Increased Competitive Fitness Compared To Nonepidemic Isolates, Catherine D. Robinson, Jennifer M. Auchtung, James Collins, Robert A. Britton

Department of Food Science and Technology: Faculty Publications

Clostridium difficile infection is the most common cause of severe cases of antibiotic-associated diarrhea (AAD) and is a significant health burden. Recent increases in the rate of C. difficile infection have paralleled the emergence of a specific phylogenetic clade of C. difficile strains (ribotype 027; North American pulsed-field electrophoresis 1 [NAP1]; restriction endonuclease analysis [REA] group BI). Initial reports indicated that ribotype 027 strains were associated with increased morbidity and mortality and might be hypervirulent. Although subsequent work has raised some doubt as to whether ribotype 027 strains are hypervirulent, the strains are considered epidemic isolates that have caused severe …

A Prospective Study Of Periodontal Disease And Risk Of Gastric And Duodenal Ulcer In Male Health Professionals, Matthew R. Boylan, Hamed Khalili, Edward S. Huang, Dominique S. Michaud, Jacques Izard, Kaumudi J. Joshipura, Andrew T. Chan

Department of Food Science and Technology: Faculty Publications

Periodontal disease is characterized by microbial shifts within the oral cavity and chronic inflammation of the gingival tissue, and can lead to tooth loss. Several studies support an association between periodontal disease and risk of chronic systemic conditions, including vascular disease, chronic obstructive pulmonary disease, diabetes mellitus, and cancer, including gastric adenocarcinoma. These associations may be mediated by systemic inflammation, the immune response to periodontal infection, or direct invasion by pathogens. Previous studies of periodontal disease and risk of peptic ulcer have been limited by cross-sectional design, diagnoses that were not confirmed by medical record review, and a lack of …

Development And Evolution Of Risk Assessment For Food Allergens, Rene W. R. Crevel, Joseph Baumert, Athanasia Baka, Geert Houben, André C. Knulst, Astrid Kruizinga, Stefano Luccioli, Stephen L. Taylor, Charlotte B. Madsen

Department of Food Science and Technology: Faculty Publications

The need to assess the risk from food allergens derives directly from the need to manage effectively this food safety hazard. Work spanning the last two decades dispelled the initial thinking that food allergens were so unique that the risk they posed was not amenable to established risk assessment approaches and methodologies. Food allergens possess some unique characteristics, which make a simple safety assessment approach based on the establishment of absolute population thresholds inadequate. Dose distribution modelling of MEDs permitted the quantification of the risk of reaction at the population level and has been readily integrated with consumption and contamination …