Food Science Commons^™

Preparation Of Immobilized Lipoxygenase Onto Magnetic Fe3O4 Nanometer Particles, Ding Wenwu, Cao Ruyi, Sun Mingzhen, Xia Yunkong

Food and Machinery

Lipoxygenase which is a kind of peroxidase could be used in many facets of industry. The stability and using times of the enzyme would be increased after the enzyme was immobilized by appropriate methods and materials. In this study, nanometer Fe₃O₄ was prepared by hydrothermal method and modified with SiO₂ and KH550 to obtained composite carriers. Lipoxygenase was immobilized on the composite carriers by glutaraldehyde. The influence factors on enzyme activity for free and immobilized enzyme were studied. The TEM results showed that the black nanoparticles were rod shaped. The mass of enzyme immobilized on the …

Effect Of Ozone Treatment On Quality Of Steamed Breads, Li Bo, Gao Xin

Food and Machinery

The wheat was treated by ozone fumigation method at concentration of 5 mg/L for 0, 0.5, 1.0, 1.5 and 2.0 h, respectively. Then the wheat flour was used to make steamed bread. The ozone treatment effect on steamed bread quality was evaluated by the specific volume, texture profile analysis, and sensory evaluation. The aspect ratio increased with the increased ozone treatment time, however, the specific volume increased first and then decreased. For the texture profile analysis, 1.0 h ozone treatment could reduce the α-amylase activity and increase the gluten content, which increased the hardness and elasticity of the steamed bread. …

Application Of New Type Of Antiseptic Natamycin In Liquid Feed, Lei Dezhu, Huang Yongjian

Food and Machinery

Inhibition effects were investigated for new type of antiseptic natamycin and the combination of natamycin and sodium diacetate on common mildews, including Aspergillus niger, Rhizopus niveus, Mucor corticolus, Penicillium glaucum. The results indicated that the minimal inhibition concentration of natamycin was 3～6 mg/L, while the optimized inhibition ratio was 4 mg/L to 3 g/L in the combination. Destructive storage test was also conducted at 10, 20 and 30 mg/L respectively in this paper, and little difference were observed according to the result by plate counting. 100% inhibition effect of natamycin at 10 mg/L could be expected to last for 18 …

Improvement And Simulation On Transmission System And Transverse Sealing Mechanism Of Automatic Vertical Packaging Machine, Liu Haisheng, Zhang Jun, Li Han, Xie Bangyin

Food and Machinery

The composition, working principle and technical requirement of automatic vertical packaging machine are discribed.The transmission systems and eccentric sprocket with variable speed are designed. Applying dimension-driven technology and “formula” function of Solidworks software, parametric design and modeling of automatic vertical packaging machine are realized. The simulation results show that larger drive sprocket limit angle can be obtained by increasing center distance of eccentric sprocket mechanism, and the heat sealing point of transverse sealing mechanism and sprocket offset corresponding packet length can be determined from the output movement characteristics of eccentric sprocket mechanism, this can improve the design quality and efficiency …

Research On Shell-Cracking Equipment And It's Working Parameter, Huang Yafang, Li Ke, Li Zongjun, Li Luoming

Food and Machinery

Bayberry seed shell-cracking equipment and it's working parameters were researched. The special equipment for that was prepared, and the optimization working parameters for bayberry seed shell cracking was achieved. Those optimal parameters are as follows: the cracking rotor runs 120r/min, the cracked rate for the first level bayberry nuclear 98.75% with the roller spacing 6.5 mm, and the naked seed obtained is 11.62%; for the second level, the cracked rate is 95.98% at roller spacing 3.5 mm, and naked seed obtained is 11.21%; for the third level, the parameters are 90.80%, 3.5 mm, and 9.91%. By statistical analysis, total shell …

Design Of Mechanical Steaming Bucket Feeding Control System Based On Plc, Li Hong, Hua Yong

Food and Machinery

Designed a PLC control system of steaming bucket feeding based on the machine vision. The feeding system can realize the artificial simulation of feeding steaming bucket when fermented grains get wet. The result indicates that this kind of operation control system is easy, safe and reliable, with low failure rate. It can greatly reduce the manual labor, and satisfies the requirements of production technology.

Effects Of Cold Storage And Shelf-Life On Physiology And Quality Of The ‘Qiufuhong’ Apples, Guo Dan, Han Yingqun, Hao Yi

Food and Machinery

Using the ‘Qiufuhong’ apples as the materials, the changes of fruit quality and physiology activity under cold storage and 7 days shelf-life after cold storage were studied. The result showed that, the ‘Qiufuhong’ apples had the respiration climacteric, the respiration climacteric of cold storage fruits happened at 98 days and the shelf-life fruits happened after 70 days cold storage, the cold storage fruits had the lower PPO activity and higher POD activity, and the activity peak were all delayed, the quality of ‘Qiufuhong’ apples became inferior and weight loss ratio enhanced quickly after 126 days cold storage and 98 days …

Study On In Vitro Antioxidant Activity Of Starfish Asterina Pectinifera Bodywall Polysaccharide Using Electron Spin, Liu Shan, Li Nan, Fu Hui, Xu Zhe, Chang Sijia, Qin Lei, Dong Xiuping, Qi Hang

Food and Machinery

The raw polysaccharide and refined polysaccharide were prepared from the body wall of fresh starfish Asterina pectinifera. The scavenging capacity of the raw polysaccharide and refined polysaccharide on hydroxyl radical,DPPH radical and superoxide anion were detected using electron spin resonance (ESR). The result indicated that the polysaccharide from the body wall of fresh starfish Asterina pectinifera possessed a certain scavenging capacity. And this scavenging capacity was enhanced with concentration rising. The IC₅₀ values of three radical scavenging activities of raw polysaccharide were 59.56,27.29,31.36 mg/mL and the IC₅₀ values of three radical scavenging activities of refined polysaccharide are 44.96,13.97,3051 …

Study On Active Antibacterial Constituents Of Chinese Herbal Formulation Extract, Liu Min, Zhou Qian, Hao Hongwei, Wang Weilin, Liu Xinyu, Zhao Wen

Food and Machinery

The research was aimed to investigate the antibacterial active ingredient of Chinese herbal formulation extract. Different concentration of ethanol was used to process the water extract of the Chinese herbal formulation, in order to remove the impurity in the water extract. Four polarity extracts were obtained by using different polarities of solvents such as n-hexane, ethyl acetate, n-butanol and water. The antibacterial ability of each polar parts was judged according to the size of the antibacterial circle. 70% concentration of ethano1can effectively remove the impurity in the water extract which can reduce the interference in the separation of active constituents. …

Optimization On Protein Extraction From Walnut Dregs, Liu Shuangfeng, Wang Huasheng

Food and Machinery

Using orthogonally rotational combination design, alkali extraction and acid precipitation was proposed for extraction of protein from walnut dregs.The optimal preparation conditions of the protein were as follows: extracting pH 9.0, extracting time 124 min, extracting temperature 53 ℃ and solid-liquid ratio 1∶22 (m∶V). Under the conditions, the yield of protein was (67.94±005)%, and its isoelectric point was pH 5.0 .

Effect Of Nitrogen Filling On Quality Of Mineral Water, Wu Musheng, Zhou Xinxin, Li Aimei, Yan Rian, Jiang Xinhui

Food and Machinery

In order to explore the effect of nitrogen filling on quality of mineral water, three kinds of mineral original water were put in airtight high pressure reaction reactor, with nitrogen filled by nitrogen cylinder. The samples under the different nitrogen pressure and pressure time were gotten by controlling the nitrogen pressure and pressure time. A series of factors were detected in the conditions, including chromaticity color, smell and taste, visible, pH, metasilicic acid, carbonates and bicarbonates, total dissolved Solids, Potassium, Sodium, Calcium, Magnesium, Strontium, water clusters. The results showed that there was no effect in mineral substance and water clusters, …

Research Progress Of Ultrasonic Effect On Protein Extraction And Modification, Wu Qian, Zhang Lifen, Chen Fusheng

Food and Machinery

Protein is the basis of life activity, and is also one of the three largest indispensable energy materials. Ultrasonic, as a physical technique, has been applied in the research of extraction and modification of large molecules such as proteins and polysaccharides. The effect of ultrasonic on the physical and chemical properties, structure and functional properties of protein was discussed, and put forward the direction and expectation.

Effect Of Aging Time And Retail Displaying Period With A Short-Term Temperature Abuse On Color Stability Of Two Beef Muscles, Derico Setyabrata, Hyun-Wook Kim, Yuan H. Brad Kim

The Summer Undergraduate Research Fellowship (SURF) Symposium

Meat color and tenderness are two most important quality factors affecting consumers’ decision on meat purchasing. Post-mortem meat aging has been widely practiced to improve palatability attributes, but could be adversely related to meat color. In particular, temperature abuse during aging or retail display can negatively affect the color stability of aged meat. The objective of this study was to determine the effect of aging time and short-term temperature abuse during display on color stability of two beef muscles (M. longissimus dorsi, LD and semitendinosus, ST). LD and ST muscles were separated from three beef carcasses, vacuum-packaged …

Processing Methods And Storage Conditions On Chocolate And Coffee Powder Flow Properties, Sunland L. Gong, Andrea Della Bella, Teresa M. Carvajal

The Summer Undergraduate Research Fellowship (SURF) Symposium

Powders are widely used in a countless number of industries, and are crucial to the quality control of products in areas such as pharmaceuticals and food. Particle physicochemical properties (morphology, solid state – crystalline, amorphous or both) are important factors for powder flow, which in turn can have significant impact on the stability, performance, and presentation of powders. Different processing methods as well as storage conditions such as relative humidity (RH) can drastically affect powder flow. Due to the widespread use of chocolate and coffee powder around the world, and their importance to the food industry, this work investigates two …

Optical Emission Spectroscopy Diagnostics Of Cold Plasmas For Food Sterilization, Abhijit Jassem, Michael Lauria, Russell Brayfield Ii, Kevin M. Keener, Allen L. Garner

The Summer Undergraduate Research Fellowship (SURF) Symposium

There is a growing need for economical, effective, and safe methods of sterilizing fresh produce. The most common method is a chlorine wash, which is expensive and may introduce carcinogens. High voltage cold atmospheric pressure plasmas are a promising solution that has demonstrated a germicidal effect; however, the responsible chemical mechanisms and reaction pathways are not fully understood. To elucidate this chemistry, we used optical emission spectroscopy to measure the species produced in the plasma generated by a 60 Hz pulsed dielectric barrier discharge in a plastic box containing various fill gases (He, N₂, CO₂, dry …

A Transcriptomal Analysis Of Bovine Oviductal Epithelial Cells Collected During The Follicular Phase Versus The Luteal Phase Of The Estrous Cycle, K. L. Cerny, E. Garrett, A. J. Walton, L. H. Anderson, P. J. Bridges

Animal and Food Sciences Faculty Publications

BACKGROUND: Reproductive success depends on a functional oviduct for gamete storage, maturation, fertilization, and early embryonic development. The ovarian-derived steroids estrogen and progesterone are key regulators of oviductal function. The objective of this study was to investigate luteal and follicular phase-specific oviductal epithelial cell function by using microarray-based transcriptional profiling, to increase our understanding of mRNAs regulating epithelial cell processes, and to identify novel genes and biochemical pathways that may be found to affect fertility in the future.

METHODS: Six normally cycling Angus heifers were assigned to either luteal phase (LP, n = 3) or follicular phase (FP, …

Determining Antimicrobial Activity In Hydrolysates Obtained From Buttermilk Substrates By Trypsin And Pepsin, Deborah Kim, Karen Ramun, Stephanie Carpintero, Suyapa Padillo, Antonio-Jose Trujillo, Rafael Jimenez-Flores

STAR Program Research Presentations

Milk proteins within their structure and sequence, contain peptides that exhibit antithrombotic, immunomodulatory, antimicrobial, antihypertensive, and other bioactivities. These peptides are released as the enzymes cleave milk proteins at certain locations of the amino acid chains. In this research, four different substrates were hydrolyzed with the gastrointestinal enzymes, pepsin and trypsin. The substrates were buttermilk (BM) and whey buttermilk (WBM), which are waste products of butter and cheese production, and supercritical CO₂ fluid (SFE) treated buttermilk (S-BM) and whey buttermilk (S-WBM). SFE acts as a solvent which removes triglycerides from the substrates and changes the overall composition of samples. …

The Effect Of Carbon Monoxide Pre-Treatments On The Colour Stability Of Vacuum Packaged Beef Steaks, L.A. Van Rooyen, P. Allen, Sarah Crawley, David I. O'Connor

Conference papers

Carbon monoxide (CO) as a component of wood smoke has a long history of applications in meat, fish, vegetable and fruit processing. More recently it has been used in meat packaging to enhance colour stability. Concerns have been raised by regulatory authorities that CO may mask meat spoilage and meat might be sold beyond its sell-by-date due to the bright red colour being retained. This study investigated the use of 5% CO as a pre-treatment prior to vacuum packaging beef striploin steaks (Longissimus thoracis et lumborum, LTL) to induce the desirable cherry red colour, while determining the optimum pre-treatment time …

Correlation Of Periodontal Pathogens In Concurrent Endodontic-Periodontal Diseases, Amela Lačević, Federico Foschi, Lejla Pojskić, Naris Pojskić, Kasim Bajrović, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Objectives: This study investigated the correlation between Tannerella forsythia, Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans at dual sites in concurrent endodonticperiodontal diseases.

Material and methods: Samples were collected from endodontium and periodontium in cases of concurrent endodontic-periodontal diseases from thirty participants. The sensitivity and specificity of SYBR Green real-time PCR was used to identify the targeted species. Absolute number of targeted genome copies in tested samples were extrapolated from respective calibration curve.

Results: No statistical difference was found in the number of detected endodontic-periodontal pathogens between the endodontium and periodontium. The Pearson test …

Ability Of The Gut Microbiota To Produce Pufa-Derived Bacterial Metabolites: Proof Of Concept In Germ-Free Versus Conventionalized Mice, Céline Druart, Laure B. Bindels, Robert Schmaltz, Audrey M. Neyrinck, Patrice D. Cani, Jens Walter, Amanda E. Ramer-Tait, Nathalie M. Delzenne

Department of Food Science and Technology: Faculty Publications

Scope—The gut microbiota is able to modulate host physiology through the production of bioactive metabolites. Our recent studies suggest that changes in gut microbiota composition upon prebiotics supplementation alter tissue levels of PUFA-derived metabolites in mice. However, in vivo evidence that gut microbes produces PUFA-derived metabolites is lacking. This study aimed to decipher the contribution of gut microbes versus that of the host in PUFA-derived metabolite production.

Methods and results—To achieve this goal, we compared the proportion of PUFA-derived metabolites and the expression of fatty acid desaturases in germ-free (GF) and conventionalized (CONV) mice fed either a low …