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Investigation Into Roseburia Intesinalis For Optimal Growth And Survivability Conditions For Potential Probiotic Use, Lisa Rana Felczak 2017 Wayne State University

Investigation Into Roseburia Intesinalis For Optimal Growth And Survivability Conditions For Potential Probiotic Use, Lisa Rana Felczak

Wayne State University Theses

Obesity and diabetes is an epidemic of increasing concern. Certain novel bacteria have been found to be an aid in reversing some of the mechanisms associated. This study focused on investigating the optimal stability conditions for R.intestinalis that can associate with an increase in growth. First, environmental conditions were tested to find the optimal conditions associated with each. Once growth conditions were found, generation time was calculated and various prebiotics were used to investigate a potential for additional growth. Next, common antibiotics were tested to see if the R.intestinalis could still show growth when exposed. Large batches of R.intestinalis were …


Development Of Gi Sustainable Probiotic Beads Using Microencapsulation, Jiarun Cui 2017 Wayne State University

Development Of Gi Sustainable Probiotic Beads Using Microencapsulation, Jiarun Cui

Wayne State University Theses

Scientists tend to pay a lot attention to probiotics in recent years, due to its health related benefits for humans. Encapsulation is a necessary way to protect probiotics from being killed by harsh conditions of the gastrointestinal tract, and to help probiotics to release in the intestinal environment. This study is mainly focusing on the encapsulation technique of Christensenella minuta, and the food product development of C. minuta beads. The extrusion technique was applied as the encapsulation method, with alginate as the encapsulation material. The effect of single encapsulation and double encapsulation of C. minuta was compared to determine the …


The Potential Stimulation Of C. Minuta Growth By Various Prebiotics, And Its Sensitivity To Various Antibiotics, Maria Elena Hakim 2017 Wayne State University

The Potential Stimulation Of C. Minuta Growth By Various Prebiotics, And Its Sensitivity To Various Antibiotics, Maria Elena Hakim

Wayne State University Theses

Gut microbiota play a crucial role in maintaining intestinal health and integrity; a feature that is both heritable, as well as affected by the environment and lifestyle. Probiotics are supplements containing live microorganisms that act in a similar manner to gut microflora, and maintain a balance in the latter. C. minuta is a novel bacterium in the gut that was found to be associated with reduction in body weight and adiposity. The aim of this study was to determine the possible effects of different prebiotics on C. minuta growth, and the survival of C. minuta in response to different antibiotics. …


Contents, Discovery Editors 2017 University of Arkansas, Fayetteville

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 18 2017, Several Authors 2017 University of Arkansas, Fayetteville

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 18 2017, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Letter From The Dean, Lona Robertson 2017 University of Arkansas, Fayetteville

Letter From The Dean, Lona Robertson

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Message From The Faculty Editor, Mary Savin 2017 University of Arkansas, Fayetteville

Message From The Faculty Editor, Mary Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Equilibrium Moisture Content Of Kabuli, Chickpea, Black Sesame, And White Sesame Seeds, Paul R. Armstrong, Elizabeth B. Maghirang, Bhadriraju Subramanyam, Samuel G. McNeill 2017 USDA Agricultural Research Service

Equilibrium Moisture Content Of Kabuli, Chickpea, Black Sesame, And White Sesame Seeds, Paul R. Armstrong, Elizabeth B. Maghirang, Bhadriraju Subramanyam, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

Sesame and chickpea are important crops in Ethiopia because both are major export crops that generate much revenue for both small farmers and the country as a whole. However, there is a lack of information about the fundamental equilibrium moisture content (EMC) relationships among these crops, which would help facilitate better monitoring and storage. Therefore, EMC adsorption and desorption prediction models based on temperature (T) and relative humidity (RH) were developed for the modified Chung-Pfost and modified Henderson models for Kabuli chickpea (KC), black sesame (BS), and white sesame (WS) seeds. The samples for conducting the adsorption and desorption tests …


Controlled Oxidative Modification With Glucose Oxidase To Enhance The Rheological And Gelling Properties Of Myofibrillar Proteins, Xu Wang 2017 University of Kentucky

Controlled Oxidative Modification With Glucose Oxidase To Enhance The Rheological And Gelling Properties Of Myofibrillar Proteins, Xu Wang

Theses and Dissertations--Animal and Food Sciences

This study investigated the feasibility of oxidative modification with glucose oxidase (GluOx) to enhance the rheological and gelling properties of myofibrillar protein. Differential oxidative modifications of myofibrillar protein (MP) by hydroxyl radicals generated in an enzymatic system with glucose oxidase (GluOx) in the presence of glucose/FeSO4 compared to a Fenton system (H2O2/FeSO4) were investigated. Firmer and more elastic MP gels were produced by the GluOx-oxidizing system than by the Fenton system at comparable H2O2 levels due to an altered radical reaction pathway.

The study further explored the effect of GluOx-mediated …


The Use Of Lactobacillus Salivarius L28 As A Bioprotective Culture In Dry Fermented Sausages, Kathy Flynt Collins 2017 University of Kentucky

The Use Of Lactobacillus Salivarius L28 As A Bioprotective Culture In Dry Fermented Sausages, Kathy Flynt Collins

Theses and Dissertations--Animal and Food Sciences

A challenge study to validate a 5 log10 CFU/g reduction of non-O157 Shiga-toxin producing Escherichia coli (STEC) in dry fermented sausage (DFS) was performed. A 4.49 ± 0.474 log10 CFU/g was achieved over two trials. The results indicated that the process was not effective in reducing the pathogen to the level required of most pathogens by the USDA.

Lactobacillus salivarius L28 (L28) was screened in vitro for the ability to inhibit STEC utilizing the paper disk diffusion method. This strain is a known bacteriocin producer. The results revealed that L28 would be a good candidate for use as …


Feeding The Soul: Voices Of Kentucky Women Combating Child Hunger, Mya Oneisha Price 2017 University of Kentucky

Feeding The Soul: Voices Of Kentucky Women Combating Child Hunger, Mya Oneisha Price

Theses and Dissertations--Community & Leadership Development

This study addresses the overarching topic of food insecurity by giving voice to individuals who are dedicating their careers toward combating child food insecurity throughout their communities. Voices are uplifted through the representation of narratives by volunteer coordinators overseeing child feeding programs, which have been established throughout Kentucky as an effort to help alleviate child hunger. This study is guided by London’s theory on career motivation, with the outcomes of this study serving as a pilot for future research centered around individuals working to combat child food insecurity. The narratives collected from this study will be used as a resource …


The Influence Of Native Woody Species, Combretum Glutinosum And Piliostigma Reticulatum, On Soil Fertility In Dialacoto, Senegal, Gwen Jacobson 2017 Michigan Technological University

The Influence Of Native Woody Species, Combretum Glutinosum And Piliostigma Reticulatum, On Soil Fertility In Dialacoto, Senegal, Gwen Jacobson

Dissertations, Master's Theses and Master's Reports

In the low-fertility soils of Senegal, West Africa, management of woody species in agricultural fields has the potential to improve soil fertility and crop production. However, optimal species for this purpose have not been clearly defined. Thus, the objective of this study was to evaluate the potential for two native woody species, Combretum glutinosum and Piliostigma reticulatum, to improve soil fertility. Soil samples were collected from beneath tree crowns of P. reticulatum and C. glutinosum and compared with fertilizer-amended and non-amended soil from adjacent open fields in a bioassay experiment. Two common crops, millet (Pennisetum glaucum) and maize (Zea mays), …


Reproductive Status Of Drosophila Suzukii (Diptera: Drosophilidae) Females Influences Attraction To Fermentation-Based Baits And Ripe Fruits, Katharine A. Swoboda-Bhattarai, Douglas R. McPhie, Hannah Burrack 2017 University of Nebraska - Lincoln

Reproductive Status Of Drosophila Suzukii (Diptera: Drosophilidae) Females Influences Attraction To Fermentation-Based Baits And Ripe Fruits, Katharine A. Swoboda-Bhattarai, Douglas R. Mcphie, Hannah Burrack

Department of Entomology: Faculty Publications

Drosophila suzukii (Matsumura) is an invasive species that is a devastating pest of soft-skinned fruit crops. Although much effort has been directed toward developing traps and attractants to monitor for D. suzukii, current monitoring tools do not reliably predict fruit infestation. The objective of this study was to determine if D. suzukii females at different developmental stages are differentially attracted to monitoring traps with fermentation-based baits and ripe fruits. Females were collected on the surface of traps, within traps, and on ripe fruits during three experiments at field locations in North Carolina, USA, and were dissected to determine their reproductive …


Surface Attachment Of Active Antimicrobial Coatings Onto Conventional Plastic Based Laminates And Performance Assessment Of These Materials On The Storage Life Of Vacuum Packaged Beef Sub-Primals, D. Clarke, A. Tyuftin, M. Cruz-Romero, D. Bolton, S. Fanning, S. K. Pankai, Carmen Bueno-Ferrer, Patrick J. Cullen, J. P. Kerry 2017 University College Cork, Ireland

Surface Attachment Of Active Antimicrobial Coatings Onto Conventional Plastic Based Laminates And Performance Assessment Of These Materials On The Storage Life Of Vacuum Packaged Beef Sub-Primals, D. Clarke, A. Tyuftin, M. Cruz-Romero, D. Bolton, S. Fanning, S. K. Pankai, Carmen Bueno-Ferrer, Patrick J. Cullen, J. P. Kerry

Articles

Two antimicrobial coatings, namely Sodium octanoate and Auranta FV (a commercial antimicrobial composed of bioflavonoids, citric, malic, lactic, and caprylic acids) were used. These two antimicrobials were surface coated onto the inner polyethylene layer of cold plasma treated polyamide films using beef gelatin as a carrier and coating polymer. This packaging material was then used to vacuum pack beef sub-primal cuts and stored at 4 °C. A control was prepared using the non-coated commercial laminate and the same vacuum packaged sub-primal beef cuts. During storage, microbial and quality assessments were carried out. Sodium octanoate treated packages significantly (p < 0.05) reduced microbial counts for all bacteria tested with an increase of 7 and 14 days, respectively compared to control samples. No significant effect on pH was observed with any treatment. The results suggested that these food grade antimicrobials have the potential to be used in antimicrobial active packaging applications for beef products.


Achieving Reactive Species Specificity Within Plasma Activated Water Through Selective Generation Using Air Spark And Glow Discharges, Peng Lu, Daniela Boehm, Paula Bourke, Patrick J. Cullen 2017 Technological University Dublin

Achieving Reactive Species Specificity Within Plasma Activated Water Through Selective Generation Using Air Spark And Glow Discharges, Peng Lu, Daniela Boehm, Paula Bourke, Patrick J. Cullen

Articles

Plasma activated liquids (PAL) attract increasing interest with demonstrated biological effects. Plasma exposure in air produces stable aqueous reactive species which can serve as chemical diagnostics of PAL systems. Here, we tailor aqueous reactive species inside plasma activated water (PAW) through treating water with AC air spark and glow discharges in contact with water. Chemical probing demonstrated species specificity between two types of PAW. Spark discharge PAW contains 2 2 H O and 3 NO , while 2 NO and 3 NO are generated in glow discharge PAW. Species formation in different PAWs have been discussed in terms of discharge …


Challenges In Model Development For Meat Composition Using Multipoint Nir Spectroscopy From At‐Line To In‐Line Monitoring, Yash Dixit, Maria Piedad Casado-Gavalda, R. Cama-Moncunill, Patrick J. Cullen, Carl Sullivan 2017 Technological University Dublin

Challenges In Model Development For Meat Composition Using Multipoint Nir Spectroscopy From At‐Line To In‐Line Monitoring, Yash Dixit, Maria Piedad Casado-Gavalda, R. Cama-Moncunill, Patrick J. Cullen, Carl Sullivan

Articles

This study evaluates the efficiency of multipoint near‐infrared spectroscopy (NIRS) to predict the fat and moisture content of minced beef samples both in at‐line and on‐line modes. Additionally, it aims at identifying the obstacles that can be encountered in the path of performing in‐line monitoring. Near‐infrared (NIR) reflectance spectra of minced beef samples were collected using an NIR spectrophotometer, employing a Fabry‐Perot interferometer. Partial least squares regression (PLSR) models based on reference values from proximate analysis yielded calibration coefficients of determination of 0.96 for both fat and moisture. For an independent batch of samples, fat was estimated with a prediction …


Fructooligosacchariedes Integrity After Atmospheric Cold Plasma And High Press, Francisca Almeida, Wesley Gomes, Rosane Souza CAvalcante, Brijesh Tiwari, Patrick J. Cullen, Jesus Maria Frias, Paula Bourke, Fabiano Fernandes, Sueli Rodrigues 2017 Universidade Federal do Ceara, Brazil

Fructooligosacchariedes Integrity After Atmospheric Cold Plasma And High Press, Francisca Almeida, Wesley Gomes, Rosane Souza Cavalcante, Brijesh Tiwari, Patrick J. Cullen, Jesus Maria Frias, Paula Bourke, Fabiano Fernandes, Sueli Rodrigues

Articles

In this study, the effect of atmospheric pressure cold plasma and high-pressure processing on the prebiotic orange juice was evaluated. Orange juice containing 7g/100g of commercial fructooligosaccharides (FOS) was directly and indirectly exposed to a plasma discharge at 70kV with processing times of 15, 30, 45 and 60s. For high-pressure processing, the juice containing the same concentration of FOS was treated at 450MPa for 5min at 11.5°C in an industrial equipment (Hyperbaric, model: 300). After the treatments, the fructooligosaccharides were qualified and quantified by thin layer chromatography. The organic acids and color analysis were also evaluated. The maximal overall fructooligosaccharides …


Laser Induced Breakdown Spectroscopy For Quantification Of Sodium And Potassium In Minced Beef: A Potential Technique For Detecting Beef Kidney Adulteration, Y. Dixit, Maria Piedad Casado-Gavalda, R. Cama-Moncunill, Xavier Cama-Moncunill, Maria Markiewicz-Keszycka, Patrick J. Cullen, Carl Sullivan 2017 Technological University Dublin

Laser Induced Breakdown Spectroscopy For Quantification Of Sodium And Potassium In Minced Beef: A Potential Technique For Detecting Beef Kidney Adulteration, Y. Dixit, Maria Piedad Casado-Gavalda, R. Cama-Moncunill, Xavier Cama-Moncunill, Maria Markiewicz-Keszycka, Patrick J. Cullen, Carl Sullivan

Articles

Beef is a rich source of important minerals, with potassium (K) being the most abundant mineral quantitatively except in cured meats where sodium (Na) from the added salt predominates. This study evaluates the capability of LIBS for quantification of the Na and K contents of minced beef as a potential method of detecting beef kidney adulteration. Additionally, the study aims to demonstrate the ability of LIBS to provide spatial mineral information of minced beef. A LIBS system was employed to collect spectral information of adulterated minced beef samples. Atomic absorption spectroscopy (AAS) was used to obtain reference values for Na …


Non-Invasive 3d And 360° Optical Imaging Of Micro-Particles, Toufic El Arnaout, Patrick J. Cullen 2017 Technological University Dublin

Non-Invasive 3d And 360° Optical Imaging Of Micro-Particles, Toufic El Arnaout, Patrick J. Cullen

Articles

Scanning electron microscopy and X-ray microtomography are useful methods for high resolution shape imaging. Visible microscopy is also common, however, developing a low-cost and customizable system for surface and shape investigation of optically active particles is challenging. In this work, we demonstrate an assembly offering good light sensitivity, flexibility of illumination and contrasts from varying angles. The design was applied, together with recent programs for focus-stacking, to analyze crystals of taurine, L-glutamic acid, acetylsalicylic acid, and copper sulfate, along with digital 3D-360° modelling of phosphorescent [Ru(bpy)3]Cl2 and strontium aluminate particles. We further tested the approach for real time monitoring of …


Optimization Of Atmospheric Air Plasma For Degradation Of Organic Dyes In Wastewater, Chaitanya Sarangapani, Yash Dixit, Vladimir Milosavljevic, Carl Sullivan, Paula Bourke, Patrick J. Cullen 2017 Technological University Dublin

Optimization Of Atmospheric Air Plasma For Degradation Of Organic Dyes In Wastewater, Chaitanya Sarangapani, Yash Dixit, Vladimir Milosavljevic, Carl Sullivan, Paula Bourke, Patrick J. Cullen

Articles

This study optimises the degradation of a cocktail of the dyes methyl orange and bromothymol blue by atmospheric air plasma. Response surface methodology (RSM) was employed to investigate the efficacy of the plasma process parameters on degradation efficiency. A Box–Behnken design (BBD) was employed to optimise the degradation of dyes by air plasma discharge. A second order polynomial equation was proposed to predict process efficiency. It was observed that the predicted values are significant (p < 0.001) with coefficients of determination 0.98, 0.96, 0.98 for dye degradation, pH value and ozone concentration, respectively. The analysis of variance results showed …


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