Impact Of Harvest Date And Year On The Volatile And Physicochemical Properties Of Fresh-Market Blackberry Cultivars,
2025
University of Arkansas, Fayetteville
Impact Of Harvest Date And Year On The Volatile And Physicochemical Properties Of Fresh-Market Blackberry Cultivars, Rahul Sen, Andrea Myers, Alyssa Finocchiaro, Gracie Howard, Luke Norman, Renee Threlfall, Amanda Mcwhirt, Margaret Worthington, Scott Robert Lafontaine
Food Science Faculty Publications and Presentations
Fresh-market blackberries face challenges with inconsistent flavor, limiting consumer demand. This study examines how harvest date and environmental conditions impact the physicochemical and volatile profiles of three cultivars (Sweet-Ark (R) Ponca, Sweet-Ark (R) Caddo, and Ouachita) grown in Fayetteville, AR, during the 2022-2023 seasons. Using targeted and untargeted GC-MS/MS approach, 262 volatile compounds across 12 classes were identified. Increased rainfall and cooler temperatures in 2023 positively affected Ouachita and Sweet-Ark (R) Caddo's physical attributes but reduced Sweet-Ark (R) Ponca's firmness and weight. Sweet-Ark (R) Caddo exhibited the highest volatile content in 2022 (2991 mu g/kg), driven by terpenes (1018.03 mu …
Electrospun Membranes From Bio-Renewable Poly (Ethylene Furanoate)/Poly (Ethylene Teraphthalate) (Pef/Pet) Blend For Emulsification Application,
2025
United Arab Emirates University
Electrospun Membranes From Bio-Renewable Poly (Ethylene Furanoate)/Poly (Ethylene Teraphthalate) (Pef/Pet) Blend For Emulsification Application, Aya Ismat Sayed
Theses
This thesis focuses on manufacturing electrospun fibrous membranes from a blend of poly (ethylene terephthalate) and poly (ethylene furanoate) and using in premix emulsification. Poly (ethylene furanoate), which is a 100% bio-renewable polymer being blended with synthetic poly (ethylene terephthalate) for membrane fabrication contributes towards sustainability in the production of food and pharmaceutical emulsions. The primary objective of this research is to optimize the fabrication conditions of poly (ethylene terephthalate)/poly (ethylene furanoate) porous membranes, characterize their properties, and evaluate their performance in emulsification. This study compares poly (ethylene terephthalate) / poly (ethylene furanoate) blend membranes with individual poly (ethylene terephthalate) …
One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review,
2025
International Centre of Insect Physiology and Ecology Nairobi
One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika
All Peer-Reviewed Publications
The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …
Encapsulation Of Lactobacillus Reuteri Dsm 17938 In W/O/W Emulsion: Influence Of Oil Type On Viability And Oxidative Stability,
2025
United Arab Emirates University
Encapsulation Of Lactobacillus Reuteri Dsm 17938 In W/O/W Emulsion: Influence Of Oil Type On Viability And Oxidative Stability, Nora Alantali
Thesis/ Dissertation Defenses
Probiotics, known for its wide range of health benefits, have been gaining recognition on its effect on the gut-brain axis. This axis, operated via various pathways, can be modulated by the type and functions of probiotics found in the gut microflora. Lactobacillus are probiotics commonly found residing in the gut and have been extensively used in food for its health benefits. However, probiotics face several stress factors before reaching the gut which hinders it from being functional. The main objective of this thesis is to construct a stable double-emulsion-based delivery system for the probiotic, which will act as a barrier …
Inactivation Of Aerosolized Hepatitis A Viral Droplets On Food Contact Surfaces By Ultraviolet-Light-Emitting Diodes At 255 Nm And 279 Nm,
2025
University of Tennessee
Inactivation Of Aerosolized Hepatitis A Viral Droplets On Food Contact Surfaces By Ultraviolet-Light-Emitting Diodes At 255 Nm And 279 Nm, Breanna Polen, Ankit Patras, Brahmaiah Pendyala, Doris H. D’Souza
Food and Animal Sciences Faculty Research
Hepatitis A viral outbreaks continue to occur. It can be transmitted through aerosolized droplets and thus can contaminate surfaces and the environment. Ultraviolet light emitting diode (UV-C LED) systems are used for inactivation of microbes, though research is needed to determine optimal doses for aerosolized HAV inactivation. This study evaluates the UV-C LED doses for the inactivation of aerosolized hepatitis A virus (HAV) deposited on stainless-steel and glass discs. HAV was aseptically deposited onto stainless-steel or glass discs (1.27 cm diameter) using a nebulizer within a chamber followed by treatments for up to 1.5 min with 255 nm (surface dose …
Effects Of Environmental Stress And Antimicrobials On The Health, Microbiome, And Product Shelf-Life Of Channel And Hybrid Catfish,
2025
Mississippi State University
Effects Of Environmental Stress And Antimicrobials On The Health, Microbiome, And Product Shelf-Life Of Channel And Hybrid Catfish, Guyue Tang
Theses and Dissertations
Two studies investigated various aspects of channel and hybrid catfish production and processing. The first study examined the effects of ammonia stress (15 mg/L vs. 1 mg/L control) on growth performance and fillet quality. While channel catfish demonstrated superior growth, ammonia stress minimally impacted the sensory profiles of both species. Gut microbiota analysis revealed species-specific differences, with Lactococcus lactis dominating hybrid catfish communities. The second study evaluated buffered vinegar treatment (1.5%) on fillet shelf life during refrigerated storage. Vinegar-treated fillets maintained better microbiological quality, with aerobic plate counts remaining below 7 log cfu/g through day 6, while untreated fillets exceeded …
The Effect Of X-Ray Irradiation Technology On Salmonella Spp. And Quality Parameters Of Ready-To-Bake Chocolate Chip Cookie Dough,
2025
Mississippi State University
The Effect Of X-Ray Irradiation Technology On Salmonella Spp. And Quality Parameters Of Ready-To-Bake Chocolate Chip Cookie Dough, Kala Grace Morris
Theses and Dissertations
Salmonella contamination in cookie dough poses a threat to consumers. This study investigated the effect of X-ray irradiation on Salmonella-inoculated ready-to-bake chocolate chip cookie dough and its impact on dough quality. A Salmonella cocktail (~10 log CFU/g) was prepared and inoculated into cookie dough samples (~6.5 log CFU/g) and then treated with X?ray irradiation (0.25, 0.5, and 1 kGy). Salmonella decreased by 1.15, 1.79, and 2.49 log CFU/g, at an exposure of 0.25, 0.5, and 1 kGy, respectively. Quality parameters (pH, aw, and colorimetry) were evaluated for two brands. No statistical difference (P>0.05) in pH was observed for Brand …
Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish,
2025
Mississippi State University
Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish, Dianna Wilson
Theses and Dissertations
The objective of this research was to investigate the biopreservative effects of chitosan and lemon essential oil in slush ice treatments on the microbial shelf life of refrigerated catfish fillets. Catfish fillets were stored in slush ice treatments containing chitosan (CH), lemon essential oil (LO), a combination (CH+LO), water (CTRL), and no slush (NS) for three d at 4 C, then stored at 4C for twelve d post-slush. The growth of background microflora in catfish fillets treated with CH and CH+LO was reduced (P<0.05), maintaining total aerobic counts below 4.5 log CFU/g, while the control reached spoilage levels (>7 log CFU/g) by Day 6. Chitosan effectively extended the microbial shelf life but lowered pH, …
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the application of a novel thermophilic lytic polysaccharide monooxygenase (LPMO ) in the efficient cellulase-mediated hydrolysis of lignocellulosic substrates.[Method ] The thermophilic enzyme mgLPMO 10,derived from a compost metagenome,was heterologously expressed in Escherichia coli (E.coli ).Its expression level was improved by modifying the signal peptide of the expression vector and induction conditions (temperature,IPTG concentration,and induction time ).The enzyme was then characterized in terms of the optimal temperature,optimal pH,thermal stability,pH stability,and the effects of metal ions on its enzyme activity.Subsequently,the synergistic hydrolysis efficiency of mgLPMO 10 with two thermophilic endoglucanases (TnCelB and DtCelB ) …
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A,
2025
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi 214122 , China
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia
Journal of Food Science and Biotechnology
[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of …
Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis,
2025
College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis, Junbao Huang, Xilin Huang, Yu Chen, Xuli Gao, Yani Luo, Wei Tao, Yongyuan Liu, Mingyu Guo, Jing Wu, Yan Liu
Journal of Food Science and Biotechnology
[Objective ] The effects of the transcription regulation system on menaquinone- 7 (MK- 7) production in Bacillus subtilis (B.subtilis ) 168 were studied by excavating transcription regulators.[Method ] With the signal molecule PhrG pentapeptide,RapG,and the transcription regulator DegU,a PhrG-RapG-DegU regulatory system was successfully constructed in B.subtilis 168 to fine-tune the expression of key genes,so that the expression of key genes in the MK- 7 synthesis pathway can respond to the changes in cell density.[Result ] The production of MK- 7 in strain BS 06 reached 102.47 mg/L through this system.[Conclusion ] Fine tuning of quorum sensing can effectively improve the …
Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg, Shengyao Wu, Zikun Ye, Weiyi Xie, Yi Wang, Haotian Ouyang, Chengcheng Zhang, Shenyan Ma
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to explore the effects of edible fungal polysaccharides on the proliferation and metabolism of Lactobacillus rhamnosus GG (LGG ).[Method ] Flarnmulina velutipes polysaccharides (FVP ),Tremella fueiformls polysaccharides (TFP),Auricularia auricula polysaccharides (AAP ),lentinan (LNT ),Hericium erinaceus polysaccharides (HEP ),and Poria cocos polysaccharides (PCP ) were selected as carbon sources for the culture of LGG.The viable bacterial count and the concentration of short-chain fatty acids (SCFAs ) were determined.Two polysaccharides were selected from the six polysaccharides and added in different mass ratios to the LGG-fermented milk,and then the pH and textural properties of the …
Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula,
2025
College of Biotechnology and Bioengineering , Zhejiang University of Technology , Hangzhou 310014 , China
Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula, Mengying Zhou, Liujie Zheng, Ruiqing Yao, Hongyu Wu, Gangliang Hu, Zhengquan Huo, Yinhua Ni
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the immunoregulatory mechanisms of lactoferrin formula and its four nutritional components (α-lactalbumin,N-acetylneuraminic acid,yeast β-glucan,and lactoferrin ) in RAW 264.7 macrophages.[Method ] M1 and M 2 macrophage polarization were induced by lipopolysaccharides (LPS) and interleukin (IL)-4,respectively.Quantitative real-time PCR,flow cytometry,and Western blot were employed to analyze macrophage polarization and related signaling pathways.[Result ] Lactoferrin formula enhanced the phagocytic activity of macrophages and dose-dependently inhibited M 1 polarization while promoting M 2 polarization within the range of 10~50 µg/mL.A combination of four nutritional components exhibited similar effects,with greater effects than the three-component combination.Additionally,the lactoferrin formula upregulated …
Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley,
2025
China Resources Snow Breweries Co ., Ltd., Beijing 100005 , China
Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley, Ran Qiu, Junhui Zhong, Xiaomin Chang, Lin Xiao, Yanlong Bai, Tengfei Yu
Journal of Food Science and Biotechnology
[Objective ] This study aims to assess the effects of Fusarium on the malting process and malt quality of barley,screen out the microorganism with inhibitory activity against Fusarium,and explore the potential of this microorganism for degrading deoxynivalenol (DON ).[Method ] The mycotoxin-producing strain was isolated from domestic Dan ’er barley contaminated with pathogenic fungi,and the interaction between the pathogen and the host was studied.The germination rate,water sensitivity,and DON mass fraction were compared between the barley infected for 5 d and standard barley.The DON mass fraction,activities of protease,β-glucanase,and amylase,and physicochemical indicators (free amino acids,soluble nitrogen,and β-glucan mass concentration ) of malt …
Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function, Lu Jin, Jiansheng Jin, Yongqiang Gu, Lulu Zhou, Feifei Han
Journal of Food Science and Biotechnology
Bile acids are the main end products of cholesterol metabolism in the body,playing an important regulatory role in the digestion and metabolism of nutrients,especially lipids.The intestine is the main organ for digestion and absorption,as well as an important site for bile acids to achieve liver-intestinal circulation.Its function is closely related to the interaction between bile acids as signaling molecules and their receptors.Focusing on the metabolism and regulatory mechanism of bile acids,the author reviews the roles and underlying mechanisms of bile acids and their receptors in regulating intestinal barriers,immune function,and gut microbiota.The aim is to provide reference for related research on …
In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight,
2025
State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight, Dingkun Zhang, Lei Wang, Ziling Yi, Beibei Wei, Deming Rao, Zhiyong Guo, Sheng Chen, Jing Wu
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to explore the effects of dextran with a small molecular weight (SDex ) on the viability of intestinal microorganisms.[Method ] The probiotic effects of laboratory-designed SDex containing only α-1,6 glycosidic bonds was evaluated by in vitro fermentation with nine typical intestinal strains,including strains from the genera Bifidobacterium and Lactobacillus,as well as species such as Escherichia coli and Enterococcus faecalis.The microbial proliferation and organic acid production during fermentation were compared among SDex,commercial dextran 20 (Dex 20),and isomalto-oligosaccharides (IMOs ).[Result ] SDex and IMOs significantly promoted the proliferation of probiotics such as Bifidobacterium and …
Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China
Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens, Miao Zou, Jingyi Zeng, Dandan Zeng, Yuhan Fu, Xu Zhang, Bo Zhao, Tianlu Mo
Journal of Food Science and Biotechnology
The CRISPR/Cas system can recognize foreign DNA,cut the target,and block or silence the expression of the foreign gene.Due to its precise targeting function,the CRISPR/Cas system has been developed as a highly efficient gene editing tool.With high specificity and operability,the CRISPR/Cas system has been combined with biosensors to improve the detection specificity and efficiency of the sensors.This review introduces the characteristics and applications of CRISPR/Cas system,analyzes the latest progress in the application of CRISPR/Cas system-based biosensors in the detection of foodborne pathogens,and summarizes and prospects the application of such biosensors.
Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou, Min Qian, Wei Zhou, Zhijin Zhou, Enbao Zhang, Jianzhong Han, Daofeng Qu
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the antibiotic resistance and transmission risk of Enterococcus faecalis (E.faecalis ) in the pork production chain.[Method ] A total of 300 samples were collected from the surface of live pigs,carcasses,and the environment in 2 pig farms,1 slaughterhouse,and 2 markets in Hangzhou,Zhejiang.The strain identification,antibiotic resistance phenotypes,resistance genotypes,and virulence genotypes were performed by 16S rDNA sequences,broth microdilution method (MIC ),and PCR.In addition,multilocus sequencing typing (MLST ) and conjugational transfer tests were conducted.[Result ] The 125 isolates of E.faecalis showed high resistance to antibiotics such as tiamulin,tetracycline,and doxycycline,and the isolates from pig farms showed higher antibiotic …
Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation,
2025
Wuxi Institute for Specialized Nutrition and Health , Wuxi 214029 , China;School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation, Huahao Cai, Linlin Wang, Qingwei Yao, Xudan Shen, Xingxing Chen, Shumao Cui
Journal of Food Science and Biotechnology
[Objective ] Functional constipation is a chronic gastrointestinal disorder caused by multiple factors,with a relatively high and increasing prevalence in China.Previous studies have found that Bacillus coagulans (B.coagulans )CCFM 1041 can alleviate symptoms of functional constipation in mice.[Method ] A double-blind,randomized controlled clinical trial was conducted to evaluate the clinical efficacy of CCFM 1041 in relieving functional constipation.A total of 73 participants were observed for changes in constipation-related indicators,serum levels of gastrointestinal regulatory neurotransmitters,and gut microbiota before and after the intervention.[Result ] After taking CCFM 1041,84.2% of participants experienced increased bowel movement frequency,and 94.7% of participants showed improved stool consistency.Compared …
Changes In Volatile Aldehydes Of Soymilk After Acid-Precipitation And Its Influencing Factors,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Changes In Volatile Aldehydes Of Soymilk After Acid-Precipitation And Its Influencing Factors, Mapeizhan Lou, Caimeng Zhang, Xiangzhen Kong, Xingfei Li, Yeming Chen, Xiaoxiao Feng, Yufei Hua
Journal of Food Science and Biotechnology
[Objective ] Acid precipitation,a common method in plant protein processing,can efficiently achieve protein enrichment and removal of non-protein components (including off-flavor substances ) on a large scale.This study aims to explore the causes for the abnormal increases in aldehydes of soymilk after acid precipitation.[Method ] Changes in volatile compounds of soymilk before and after acid precipitation were analyzed by gas chromatography-mass spectrometry (GS-MS ).Soymilk components were fractionated by centrifugation,and the contributions of different fractions to the increases in aldehydes were investigated.The changes in the molar concentrations of lipid hydroperoxides before and after acid precipitation were determined,and a molar ratio between …
