Structural And Solution Property Characterization Of Oat Β-Glucans,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao
Journal of Food Science and Biotechnology
[Objective ] This study aims to characterize the structure and investigate the solution properties of oat β-glucan (OBG ).[Method ] Two soluble OBGs with different weigh-average molecular weights (Mw),number-average molar weights (Mn),and peak molecular weights (Mp) were prepared by hot water extraction and ethanol classification.The structures and solution properties were characterized by Fourier-transform infrared spectroscopy (FT-IR ),nuclear magnetic resonance (NMR ),X-ray diffraction (XRD ),scanning electron microscopy (SEM ),high performance size exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS ),and rheology.[Result ] OBG- 1 and OBG- 2 showed the Mw of 80.73×104 g/mol and 8.05×104 g/mol,the yields of (4.86±0.55)% and …
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots,
2025
Institute of Sea Food , Zhejiang Gongshang University , Hangzhou 310012 , China
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish a rapid immunoassay method for the quantitative detection of eugenol.[Method ] The eugenol monoclonal antibody was conjugated to the surface of quantum dots by the 1-ethyl- 3- (3-dimethylaminopropyl ) carbodiimide (EDC ) and N-hydroxy-succinamide (NHS ) (EDC-NHS ) coupling method,with quantum dots as fluorescent markers.Bovine serum albumin (BSA ) was used for blocking,and fluorescence microscopy,agarose gel electrophoresis,and a microplate reader were used for identification.Furthermore,the established method was compared with high performance liquid chromatography (HPLC ).[Result ] The optimal synthesis conditions were as follows:quantum dot activation time of 30 min,conjugation pH with 8.0,antibody addition …
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch,
2025
College of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464000 , China
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the properties of chestnut starch.[Method ] Tea polysaccharides were added to chestnut starch in different proportions,and the effects of tea polysaccharides on the water-holding capacity,digestive properties,textural properties,and other properties of chestnut starch were investigated.[Result ] The water-holding and oil-holding properties of chestnut starch were the largest when tea polysaccharides were added at 1% and then gradually decreased with the increase in the proportion of tea polysaccharides added.The transparency of chestnut starch gradually increased when tea polysaccharides were added from 1% to 8% and decreased significantly when tea polysaccharides were added at 16%.Adding tea …
Research Progress In Thermo-Reversible Polysaccharide Gels,
2025
State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai
Journal of Food Science and Biotechnology
Thermo-reversible gels are widely applied in the processing of baking,meat,and snack products including cakes,candies,jelly,aspic,and steamed custard buns.Currently,the most effective thermo-reversible gel is gelatin,which can combine with water to form a transparent gel that melts when it is close to the temperature of the human oral cavity.However,gelatin is expensive.Therefore,researchers have turned their attention to polysaccharide gels,hoping to obtain more thermo-reversible food raw materials with different properties.The author reviews thermo-reversible polysaccharide gels from natural sources such as plants,algae,and microorganisms and those modified by physical,chemical,and enzymatic methods and summarizes the sources,structures,gelatinization mechanisms,and product applications of polysaccharide gels.It is expected to provide a reference …
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases,
2025
Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming 650500 , China
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li
Journal of Food Science and Biotechnology
The antioxidant properties of lactic acid bacteria are related to various probiotic activities,which can prevent and alleviate a variety of chronic diseases caused by oxidation in the host.The antioxidant mechanisms of lactic acid bacteria,including the action mechanisms of antioxidant enzymes and non-enzymatic antioxidants and regulation of host signaling pathways,were expounded.Furthermore,the application status of antioxidant properties of lactic acid bacteria in diseases of the nervous system,digestive system,and skin system were summarized.The aim is to explore how to enhance the antioxidant activity of lactic acid bacteria in oxidative stress-related diseases and give new insights into the development of related treatment strategies.
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu
Journal of Food Science and Biotechnology
[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- …
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China;Shanghai Engineering Research Center of Food Microbiology , University of Shanghai for Science and Technology , Shanghai 200093 , China
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai
Journal of Food Science and Biotechnology
[Objective ] The extraction efficiency of different methods and the effects of these methods on the composition of mannoproteins extracted from yeast cell walls were compared.Furthermore,the anti-inflammatory activities of the extracted mannoprotein were evaluated.[Method ] Three yeast mannoproteins (MP-W,MP-B,and MP-E ) were extracted via high-temperature water,alkali,and enzyme-assisted extraction methods and purified via ethanol precipitation.The identification of monosaccharide composition and amino acid composition,distribution of relative molecular mass,and Fourier transform infrared spectroscopy were employed to analyze the composition and chemical structures of yeast mannoproteins.A cell experiment was carried out to examine the anti-inflammatory activity.[Result ] The yields of yeast mannoproteins extracted by …
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging,
2025
Yunnan Botanee Bio-technology Group Co ., Ltd ., Kunming 650106 , China;Shanghai Botanee Bio-technology Group Co ., Ltd ., Shanghai 201702 , China
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging, Shishuai Yu, Yining Hao, Bo Peng, Youyun Liu, Liping Qu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE ) and its Inhibitory effect on cell aging.[Method ] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC ).The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT ),respectively.After the aging model of fibroblasts was established,the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay,and the expression of matrix metalloproteinase- 1 (MMP- 1),matrix metalloproteinase- 3 …
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase, Chuanxiang Wei, Keru Chen, Yun Hu, Chao Sun, Rukang Xue, Fubao Sun, Jingfang Cheng
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish the biosynthesis process of N-acetyl- trans -4-hydroxyproline (N-ATHOP ).[Method ] Escherichia coli (E.coli ) BL21 (DE3) was selected as the host to heterologously express the acyltransferase (MsAcT ) derived from Mycobacterium smegmatis.A semi-rational design was employed to construct the mutant MsAcT (S11C) capable of catalyzing the synthesis of N-ATHOP.[Result ] The catalytic reaction conditions of the mutant MsAcT (S11C) were optimized as pH 6.9,reaction temperature 49 ℃,initial trans -4-hydroxyproline (THOP ) concentration of 315.5 mmol/L,and vinyl acetate volume fraction of 10%.Under the optimized conditions,the yield of N-ATHOP synthesized under the catalysis of MsAcT …
Zeaxanthin Encapsulated With High Internal Phase Emulsions And Its Stability,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China
Zeaxanthin Encapsulated With High Internal Phase Emulsions And Its Stability, Ya Li, Ying Ou, Jie Lu, Shuo Meng, Shoushuai Feng, Hailin Yang
Journal of Food Science and Biotechnology
[Objective ] This study prepared highly stable high internal phase emulsions (HIPEs ) to encapsulate zeaxanthin,thereby improving the stability and expanding the food and pharmaceutical applications of zeaxanthin.[Method ] HIPEs were prepared with the gelatin-chitosan suspension for encapsulation of zeaxanthin extracted from goji berries.Ultra-performance convergence chromatography (UPCC ) was employed to separate zeaxanthin.Confocal laser scanning microscopy (CLSM ) was employed to observe the emulsion structure.The rheological analysis was performed to characterize the emulsion,which was combined with the hydrogen peroxide and storage stability tests to evaluate the performance of encapsulation with HIPEs in improving the stability of zeaxanthin.[Result ] The separation …
Health Risk Assessment Of Multiple Mycotoxins In Rice , Maize , Soybean , And Wheat Flour Marketed In Shanghai,
2025
The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Health Risk Assessment Of Multiple Mycotoxins In Rice , Maize , Soybean , And Wheat Flour Marketed In Shanghai, Zuoyin Zhu, Jie Wang, Junmei Ma, Hanke Zhao, Haisheng Cheng, Chengbao Huang, Junhua Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the potential health risks to consumers from exposure to multiple mycotoxins in grains.[Method ] Point and probabilistic assessment models were adopted to analyze the dietary exposure risks of adult residents in Shanghai to aflatoxins (AFs),ochratoxin A (OTA ),zearalenone (ZEN ),deoxynivalenols (DONs ),and fumonisins (FBs) through consumption of rice,maize,soybeans,and wheat flour.[Result ] The margin of exposure values (MoE ) for AFs in maize and aflatoxin G 1 (AFG 1) in soybeans exceeded 10 000,indicating no dietary exposure risk.In contrast,AFs in rice and wheat flour,as well as aflatoxin B 1 (AFB 1),aflatoxin B 2 (AFB …
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles,
2025
Yueyang Inspection and Testing Center ,Yueyang 410042 ,China;Hunan Provincial Pickles Product Quality Supervision and Inspection Center , Yueyang 410042 , China
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles, Li Zhu, Huimin Li, Liejiang Yuan, Cancan Peng, Bo Zhou, Sheng Song, Haizhi Wu, Mingyang Zhu, Fangmei Wang, Yu Liu, Yuqian Hu, Ge Li, Yiqing Sun, Yi Zhou
Journal of Food Science and Biotechnology
[Objective ] This study aims to identify the main spoilage bacteria and evaluate their spoilage capacity in the spoilage process of pickles.[Method ] High-throughput sequencing was employed to analyze the microbial community dynamics in the samples of pickles at the early,middle,and late stages of spoilage.The key spoilage bacteria were isolated,purified,and then identified through a combination of colony morphology observation,biochemical analysis,and 16S rDNA/ITS sequencing.Re-inoculation experiments were carried out for further validation of the primary spoilage bacteria in pickles.[Result ] Acinetobacter and Bacillus were the dominant spoilage bacterial genera across the three spoilage stages.Notably,Bacillus exhibited rapid growth over time,with the relative abundance …
Establishment And Application Of An Immunomagnetic Bead-Based Melisa Method For Olaquindox,
2025
Institute of Sea Food , Zhejiang Gongshang University , Hangzhou 310012 , China
Establishment And Application Of An Immunomagnetic Bead-Based Melisa Method For Olaquindox, Ziyi Chen, Yanling Song, Junjie Sun, Yinlai Li, Lu Zhai, Yaofeng Zhang, Junli Feng, Renyao Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish a magnetic bead enzyme-linked immunosorbent assay (MELISA ) method for olaquindox (OLA ) based on immunomagnetic beads.[Method ] First,olaquindox-hapten conjugated with horseradish peroxidase (OLA-HS-HRP ) and immunomagnetic beads were synthesized.Then,the conjugated immunomagnetic beads and OLA-HS-HRP were used to screen and optimize the reaction conditions of MELISA.[Result ] The optimal working dilution factors for the immunomagnetic beads and OLA-HS-HRP were 100 times and 8 000 times,respectively,with the antibody dilution buffer being PBS solution (0.010 mol/L,pH 7.0) containing methanol at a volume fraction of 5%.Under the optimized conditions,the established standard equation was y=13.593ln x+17.173 (R2=0.994 …
Establishment And Application Of A Uplc-Ms/Ms Method For Simultaneous Determination Of 39 Veterinary Drugs And Their Metabolites In Black Bone Chicken,
2025
School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Establishment And Application Of A Uplc-Ms/Ms Method For Simultaneous Determination Of 39 Veterinary Drugs And Their Metabolites In Black Bone Chicken, Jiawei Du, Yongqin Li, Shuangyuan Jin, Xueyan Wang, Hongwu Wen, Xuelian Guo, Hao Li, Lihua Xu
Journal of Food Science and Biotechnology
[Objective ] This study aims to comprehensively investigate the risk of veterinary drug residues in black bone chicken.[Method ] A method for rapid and simultaneous analysis of 39 veterinary drugs and their metabolites in three categories was established based on low-temperature ultrasonic extraction,solid-phase extraction pretreatment,and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ).The samples were extracted by Mcllvaine-Na 2EDTA and phosphate buffer via ultrasonic treatment at low temperatures,purified by Oasis HLB solid-phase extraction column,separated by T 3 chromatographic column,and quantified by the matrix matching external standard method.[Result ] The 39 veterinary drugs and their metabolites showed good linear relationships in …
Covid-19-Related Beliefs And Dietary Behaviors Of American Undergraduate Students Vary By Race Via The Lens Of The Health Belief Model,
2025
Montclair State University
Covid-19-Related Beliefs And Dietary Behaviors Of American Undergraduate Students Vary By Race Via The Lens Of The Health Belief Model, Doreen Liou, Jong Min Lee
Department of Nutrition and Food Studies Scholarship and Creative Works
The COVID-19 pandemic caused immense physical disruptions, affecting young adults in the U.S. The Health Belief Model is a social psychological framework that predicts the likelihood of adopting health behavior. The purpose of this research is to investigate COVID-19-related health beliefs and dietary behaviors among undergraduate students during the pandemic. Using convenience sampling, a cross-sectional survey was completed by 304 individuals at a New Jersey state university. Survey data included the frequency of COVID-19 prevention behaviors (e.g., wearing an indoor mask, handwashing), and consumption of fruit and vegetables. The Health Belief Model constructs measured perceived susceptibility to COVID-19, severity, benefits, …
Protein Supplementation, Plasma Branched-Chain Amino Acids, And Insulin Resistance In Postmenopausal Women: An Ancillary Study From The Supplemental Protein To Outsmart Osteoporosis Now (Spoon) Trial,
2025
Montclair State University
Protein Supplementation, Plasma Branched-Chain Amino Acids, And Insulin Resistance In Postmenopausal Women: An Ancillary Study From The Supplemental Protein To Outsmart Osteoporosis Now (Spoon) Trial, Jessica Dauz Bihuniak, Alessandra Byer, Christine A. Simpson, Rebecca R. Sullivan, Josephine M. Dudzik, Karl L. Insogna, Jeannette M. Beasley
Department of Nutrition and Food Studies Scholarship and Creative Works
Background/Objectives: Studies have reported an increased risk of type 2 diabetes among people with higher protein intake. Moreover, branched-chain amino acids (BCAA) are reported to be positively associated with insulin resistance (IR). However, it is not understood whether elevated levels of BCAA are causal to IR development, or if higher BCAA are a marker of IR. The objective of this study was to examine the effects of long-term protein and carbohydrate supplementation on plasma BCAA levels, and the relationship between plasma BCAA and IR in postmenopausal women. Methods: Stored samples and data from 84 postmenopausal women who participated in a …
Studying The Population Dynamics Of Nslab And Their Influence On Spores During Cheese Ripening,
2025
South Dakota State University
Studying The Population Dynamics Of Nslab And Their Influence On Spores During Cheese Ripening, Rakesh Kaushik, Sanjeev Anand
Dairy Science and Food Science Faculty Publications: 2022 to Present
Cheese ripening involves microbial changes, with starter lactic acid bacteria (SLAB) initiat- ing fermentation and nonstarter lactic acid bacteria (NSLAB) driving flavor and texture development. However, heat-resistant spores of Clostridium and Bacillus can survive pas- teurization and cause spoilage during ripening. This study evaluated NSLAB dynamics in the presence of spores during cheese ripening. Cheddar cheese samples at pilot-scale level (110 L) with four treatments, namely control, with spores of B. licheniformis (T1), with spores of Cl. tyrobutyricum (T2), and both spores (T3) at 2.0 Log10 CFU/mL, were ripened at 7 ◦C for six months. SLAB declined from 8.0 to …
Hyflex Teaching Application In Beverage Npd,
2025
Technological University Dublin
Hyflex Teaching Application In Beverage Npd, Catherine (Rena) Barry-Ryan
Case studies: Digital Education
The post-graduate module (Level 9) in Beverage Product Development (FOOD8003) is delivered to a wide range of students including mature, international and those working full-time. This hyflex module was delivered in Semester 2 of their year-long courses, where students were well settled in TU Dublin, had gained technological ability and were familiar with the VLE (Brightspace) and its online classroom (Bongo). Each week the module progresses a step in the new product development process and therefore attendance and engagement in weekly activities were essential. The student could choose to attend face-to- face or online each week, while getting the same …
Cazyme Gene Cluster Diversity In Human Gut Microbiome,
2025
University of Nebraska-Lincoln
Cazyme Gene Cluster Diversity In Human Gut Microbiome, Yi Xing
Department of Food Science and Technology: Dissertations, Theses, and Student Research
In gut microbiome research, carbohydrate-active enzyme gene clusters (CGCs) have emerged as key functional units for understanding microbial glycan degradation. Unlike taxonomic or broad pathway annotations, CGCs offer gene-cluster-level resolution and capture substrate-specific microbial functions. However, their diversity and distribution in relation to host metabolic phenotypes, such as obesity, remain poorly characterized. This study tests the hypothesis that the composition and abundance of fiber-targeting CGCs vary between obese and healthy human gut microbiomes, reflecting distinct microbial carbohydrate utilization strategies. To examine this, we constructed a high-quality reference CGC dataset comprising 94,019 clusters from the Unified Human Gastrointestinal Genome and profiled …
Germination Time: Impact On Nutritional, Thermal, And Paste Properties Of Red Rice,
2025
Univ Fed Pelotas
Germination Time: Impact On Nutritional, Thermal, And Paste Properties Of Red Rice, Thauana Heberie, Lucas Avila Do Nascimento, Ya-Jane Wang, Rosana Colussi, Jaqueline Ferreira De Souza, Andre Ricardo Fajardo, Marcia Foster Mesko
Food Science Faculty Publications and Presentations
BACKGROUNDThe study investigates how varying germination times affect the nutritional, thermal, and pasting properties of red rice. Germination was conducted over 32 h, with aliquots collected every 2 h starting from 18 h for property evaluation.RESULTSAt all evaluated germination times, the red rice samples exhibited low to intermediate amylose levels, while resistant starch content remained relatively constant (approximately 15-20%). Structural changes in the starch were observed: the peak viscosity decreased, maximum gelatinization of the granules occurred at 76 degrees C, and spectroscopic bands characteristic of this polysaccharide were detected at 3400 cm-1 (O-H stretching) and within the 1150-1000 cm-1 range …
