Food Science Commons^™

A Multi-Analyte Lc-Ms/Ms Method For Screening And Quantification Of Nitrosamines In Sartans, Shu-Han Chang, Ching-Chia Chang, Li-Jing Wang, Wei-Ching Chen, Shu-Yu Fan, Chi-Zong Zang, Ya-Hui Hsu, Mei-Chih Lin, Su-Hsiang Tseng, Der-Yuan Wang

Journal of Food and Drug Analysis

An incident of sartan medicine contamination was notified by Europe in June 2018. The contaminant was identified as a probable carcinogenic nitrosamine and the recalls of sartan medicines were soon made. Since then, more nitrosamine contaminants in sartan medicines were reported. To broaden the applicability and variety in nitrosamine determination, a multi-analyte method is required. In this study, a feasible and sensitive multi-analyte LC-MS/MS method for determination of 12 nitrosamines in sartans was established, where the active pharmaceutical ingredients and final products merchandised in Taiwan were also examined. Chromatographic separation was achieved on an Xselect® HSS T3 column (15 cm …

Anti-Inflammatory Effects Of Peptides From A Marine Algicolous Fungus Acremonium Sp. Ntu492 In Bv-2 Microglial Cells, George Hsiao, Shih-Wei Wang, Yin-Ru Chiang, Wei-Chiung Chi, Yueh-Hsiung Kuo, Do Anh Phong, Chia-Yu Chen, Tzong-Huei Lee

Journal of Food and Drug Analysis

Located in tropical and subtropical region, Taiwan is an island with high algal species diversity. In this study, a number of fungal strains were isolated from marine macroalgae collected from northeastern intertidal zone of Taiwan. Preliminary anti-inflammatory screening has shown that the methanolic extracts of solid fermented products of the red alga Mastophora rosea-derived fungal strain Acremonium sp. NTU492 exhibited significant bioactivity. In an attempt to disclose the active principles from this fungal strain, a series of separation and purification was thus undertaken, which has led to the isolation and characterization of seven compounds including four new peptides, namely acrepeptins …

A Rapid Strategy For Screening High-Efficiency Pcsk9 Inhibitors From Ginkgo Biloba Leaves By Ligand Fishing, Hplc-Q-Tof-Ms And Interdisciplinary Assay, Li Li, Meng-Lin Fan, Ya-Nan Li, Ya-Xuan Huang, Xiu-Feng Liu, Ji-Hua Liu

Journal of Food and Drug Analysis

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an attractive target for new cholesterol-lowering drug development. Here, we developed a method integrating ligand fishing, HPLC-Q-TOF-MS and interdisciplinary assay, aiming to explore potential PCSK9 inhibitors from mixtures rapidly and accurately. PCSK9 was expressed and purified firstly, and then the recombined PCSK9 was coated on the surface of magnetic beads (MBs). The PCSK9-immobilized MBs (PCSK9-MBs) were used for ligand fishing combined with HPLC and Q-TOF-MS/MS. Ginkgo biloba leaves (GBL), an herbal medicine widely used in Asia and Europe with good efficacy in treatment of hypercholesterolemia, were chosen as an illustration for ligand fishing. …

Analysis Of Flavor And Other Metabolites In Lemon Juice (Citrus Limon) From Huanglongbing-Affected Trees Grafted On Different Rootstocks, Laura Reuss, Shi Feng, Wei-Lun Hung, Qibin Yu, Frederick G. Gmitter Jr, Yu Wang

Journal of Food and Drug Analysis

Flavor perception integrates sensory input from chemical receptors triggered by taste- and aroma-active metabolites to contribute to food flavor quality. Many factors alter flavor quality. Disease affects aroma and flavonoid constituents, causing off-flavors in plants. Huanglongbing (HLB) disease negatively affects citrus, although lemons are more tolerant. Lemon juice quality of HLB-affected fruits is not well studied. Lemon juice aroma-active compounds were profiled in this study using gas chromatography/mass spectrometry-olfactometry, and other metabolites contributing to overall flavor quality were investigated. Lemon juice from different rootstocks was discriminated using the metabolic profile. Flavor and other lemon juice metabolites also distinguished symptomatic from …

Chemometric Authentication Of Pu'er Teas In Terms Of Multielement Stable Isotope Ratios Analysis By Ea-Irms And Icp-Ms, Honglin Liu, Yitao Zeng, Xin Zhao, Huarong Tong

Journal of Food and Drug Analysis

In this work, the stable isotope ratios of carbon, nitrogen, hydrogen, oxygen, and mineral elements and their stoichiometric methods were examined as possible factors that could certify Chinese tea based on its production years. A total of 43 multi-element stable isotope ratios of Xiangzhujing Pu'er tea in five production years were determined through inductively coupled plasma mass spectrometry (ICP-MS) and elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) methods. Two unsupervised learning techniques (principal component analysis and hierarchical clustering analysis) and three supervised learning techniques (partial least squares discriminant analysis [PLS-DA], back-propagation artificial neural network [BP-ANN], and linear discriminant analysis [LDA]) were …

The Chemical Aspects Of Raman Spectroscopy: Statistical Structure-Spectrum Relationship In The Analyses Of Bioflavonoids, Chih-Hsien Wang, Chia-Chi Huang, Wenlung Chen, Yen-Shi Lai

Journal of Food and Drug Analysis

Raman spectroscopy has been accepted as a useful tool for the characterization of natural products. However, to identify a specific compound in a mixture sample of natural products using Raman spectra alone is highly challenging if not impossible. We demonstrated an effective solution to such issues using a method combining statistical Raman spectroscopy and Mass spectrometry. The method was validated with a successful application to the identification of the major anthocyanin components in a purple yam (Dioscorea purpurea) extract. Of particular interest is that statistical grouping of the bioflavonoid standards that formed the database of this study was found to …

Screening For Malachite Green Contamination On Live Fish Skin With Chewing Gum Based Viscoelastic Sers Sensor, Wan-Li Fan, Shi-Wei Yang, Dong-Mei Wang, Zheng-Jun Gong, Mei-Kun Fan

Journal of Food and Drug Analysis

Malachite green (MG), a prohibited but still found antimicrobial in aquafarm and during live fish shipping, is a hot target in food safety screening. Herein, a novel chewing gum based flexible SERS (G-SERS) sensor was proposed for rapid sampling and detection of MG on live fish skin. The whole analysis takesmin, while the limit of detection for MG is 0.73 pg. Different from other reports, MG contaminated live fish was monitored daily with the G-SERS sensor, during which the fish was firstly raised in 0.5 ppm MG solution for one day, followed by freshwater for a week. It was found …

Uplc-Qtof-Ms Fingerprinting Combined With Chemometrics To Assess The Solvent Extraction Efficiency, Phytochemical Variation, And Antioxidant Activities Of Beta Vulgaris L., Junyi Wang, Guddadarangavvanahally Krishnareddy Jayaprakasha, Bhimanagouda S. Patil

Journal of Food and Drug Analysis

Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and golden beets (Beta vulgaris L.), using twenty solvent (S1eS20) mixtures containing water, methanol, and ethanol alone or with acids (ascorbic, formic, acetic). Red beetroot extracted with methanol with or without acid had the highest betanin content (2791.0 mg/g and 8222.3 mg/g of fresh weight [FW], respectively) and golden beetroot extracted with methanol/ascorbic acid/water had the highest vulgaxanthin I (193.7 mg/g and 15.0 …

Food Preservative Sorbic Acid Deregulates Hepatic Fatty Acid Metabolism, Chia-Hui Chen, Sin-Ni Ho, Po-An Hu, Yu Ru Kou, Tzong-Shyuan Lee

Journal of Food and Drug Analysis

Sorbic acid (SA) is one of the most commonly used food preservatives worldwide. Despite SA having no hepatotoxicity at legal dosages, its effect on hepatic lipid metabolism is still unclear. We investigated the effect of SA on hepatic lipid metabolism and its mechanism of action in C57BL/6 mice. Daily treatment with SA (1 g/kg in diet) for 4 weeks did not alter the body weight, organ weight, and blood lipids in mice. However, hepatic lipid accumulation, particularly that of triglycerides, fatty acids, and glycerol, but not cholesteryl ester and free cholesterol, was increased with SA treatment. Mechanistically, SA decreased the …

The Potential Role Of Phenolic Compounds On Modulating Gut Microbiota In Obesity, Gow-Chin Yen, Hsin-Lin Cheng, Li-Yu Lin, Shiuan-Chih Chen, Chin Lin Hsu

Journal of Food and Drug Analysis

Obesity is a rising public health issue and challenge which is tightly correlated with socio-economic development paralleled with increased energy intake and sedentary behavior that subsequently cause adipose tissue accumulation. Physiological and metabolic status changes during obesity development have been suggested with low grade inflammation of gastrointestinal tract. The gut microbiota plays an essential role in regulating whole body energy metabolism and also lipid accumulation, and immunity of host. However, the detail mechanism of which the gut microbiota composition influence obesity development in humans still need deeper investigation owing to the complex pathophysiology of such disease. Recently, the consumption of …

Improving Microbiological Safety Of Low Moisture Food Products Using Radio Frequency And Ethylene Oxide, Long Chen

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Recent foodborne illness outbreaks in US associated with consumption of low-moisture foods (LMF) have heightened concerns of their microbial safety. Salmonella is a pathogen of major concern in LMF due to its ability to persist in low water activity (a_w) environments. The disadvantages of existing decontamination technologies for LMF call for novel and efficient intervention technologies. Radio frequency (RF) and ethylene oxide (EtO) were evaluated in this dissertation for improving microbial food safety and quality of LMF. Cumin seeds and inshell hazelnuts were selected as model foods.

It took < 2 min of stationary RF heating to achieve > 5 log reductions of Salmonella in cumin seeds without …

Self-Efficacy And Psychosocial Considerations Of Obesity Risk Reduction Behaviors In Young Adult White Americans, Doreen Liou, Lauren Kulik

Department of Nutrition and Food Studies Scholarship and Creative Works

The obesity epidemic is a pervasive health issue affecting all population groups in developed countries. The purpose of this research was to ascertain obesity risk reduction behaviors and their psychosocial determinants in young adult Americans residing in New Jersey state. A cross-sectional survey design was implemented in which a convenience sample of 174 participants (18 to 40 years) completed a validated online self-administered questionnaire. Nineteen obesity risk reduction behaviors, self-efficacy and psychosocial constructs derived from the Theory of Planned Behavior were measured. Statistical analyses were conducted using frequency distributions, t-tests and regression analysis. Regression analysis indicated that 37.5% of the …

Hulumtimi Rreth Llojeve Të Ambalazheve Dhe Roli I Tyre Në Industrinë Ushqimore, Xheneta Luma

Theses and Dissertations

Sot, ambalazhimi i ushqimit është pranuar si një strategji për rritjen e konkurrencës së produkteve ushqimore nga ekspertët. Ambalazhimi i ushqimit është mbyllja e ushqimit për ta mbrojtur atë nga dëmtimi, ndotja, prishja, dëmtuesit, gjatë transportit, magazinimit dhe shitjes me pakicë. Zhvillimi i materialeve të ambalazhimit tregon zhvillimin paralel me teknologjinë. Sidomos rritja e numrit të të punësuarve, veqanërisht rritja e punësimit të femërave gjithnjë e më shumë e rriti kërkesën për ushqim të gatshëm. Rritja e kërkesës për ushqim të përgatitur lehtësisht solli teknologjinë e re të ambalazhimit dhe funksionimi i ambalazhimit është rritur. Prodhimi i ambalazheve për konfeksionimin …

Hulumtimi Rreth Llojeve Të Ambalazheve Dhe Roli I Tyre Në Industrinë Ushqimore, Xheneta Luma

Theses and Dissertations

Sot, ambalazhimi i ushqimit është pranuar si një strategji për rritjen e konkurrencës së produkteve ushqimore nga ekspertët. Ambalazhimi i ushqimit është mbyllja e ushqimit për ta mbrojtur atë nga dëmtimi, ndotja, prishja, dëmtuesit, gjatë transportit, magazinimit dhe shitjes me pakicë. Zhvillimi i materialeve të ambalazhimit tregon zhvillimin paralel me teknologjinë. Sidomos rritja e numrit të të punësuarve, veqanërisht rritja e punësimit të femërave gjithnjë e më shumë e rriti kërkesën për ushqim të gatshëm. Rritja e kërkesës për ushqim të përgatitur lehtësisht solli teknologjinë e re të ambalazhimit dhe funksionimi i ambalazhimit është rritur. Prodhimi i ambalazheve për konfeksionimin …

Broccoli, Lisa Lewis, Emily Morales, Heidi Leblanc

All Current Publications

This guide to eating, selecting, cooking, and storing broccoli.

Beets, Janet Smith, Anita Raddatz, Heidi Leblanc

All Current Publications

This publication is part of the Food Sense Guide to Eating Fresh Fruits and Vegetables and includes information on selecting, preparing, cooking, and storing beets.

Banana, Marie Stosich, Emily Morales, Heidi Leblanc

All Current Publications

This publication guide to eating, selecting, cooking, and storing bananas.

Role Of Gut Microbiota In Anti-Colitic Effects Of Color-Fleshed Potatoes, Lavanya Reddivari, Shiyu Li, Tianmin Wang, Mary Kennett, Jairam Vanamala

Department of Food Science Faculty Publications

The prevalence of ulcerative colitis (UC), a chronic inflammatory bowel disease, is on the rise with ∼700,000 patients in the US alone in 2018. Gut bacterial dysbiosis plays an important role in ulcerative colitis. We have recently shown that anthocyanin-containing potatoes exert anti-inflammatory activity in colitic mice. However, no information is available on whether gut bacteria play a role in the anti-colitic activity of color-fleshed potatoes. This study examined the anti-colitic activity of red/purple-fleshed potatoes in mice with intact and antibiotic-ablated microbiome.

Effect Of Pecan Variety And The Method Of Extraction On The Antimicrobial Activity Of Pecan Shell Extracts Against Different Foodborne Pathogens And Their Efficacy On Food Matrices, Veerachandra K. Yemmireddy, Cameron Cason, Juan Moreira, Achyut Adhikari

School of Earth, Environmental, & Marine Sciences Faculty Publications

The shells of pecans are a rich source of bioactive compounds with potential inhibitory activity against various pathogenic microorganisms. This study investigated the antimicrobial activity of pecan shell extracts as effected by the type of cultivar and the method of extraction against various foodborne bacterial pathogens. Defatted shell powders of 19 different pecan cultivars were subjected to aqueous and ethanolic extraction (1:20 w/v) procedures, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lyophilized pecan shell extracts dissolved in deionized water containing 5% DMSO (v/v) were determined against multiple strains of Listeria monocytogenes, Salmonella enterica, and …

Study Of In Vitro Bioaccessibility And In Vivo Bioavailability Of Khalas Variety Date Seed Polyphenols For Functional Application In The Human Diet, Serene Hilary

Food Science Dissertations

Date seeds are an ideal source of polyphenols for the food industry. This doctoral work investigated Khalas variety date seeds in three forms, as powder (DSP), ethanolic extract (DSE) and functional food developed with the seeds; pita bread (DSB). The study characterised the polyphenol profile by mass spectrometry and investigated the bioaccessibility using simulated in vitro digestion coupled with Caco- 2 model for absorption and transport. It further explored the bioavailability of date seed polyphenols in vivo in humans by conducting a cross-over study in 16 healthy volunteers. The crossover study explored the urinary excretion of polyphenol metabolites, and the …