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Establishment Of Arapid Detection Method For Carbendazim Enzyme-Linked Immunosorbent, JIANG Wen-hui, WU Xiao-sheng, CUI Na, ZHANG Yu, PENG Zheng-xue, CUI Hai-feng, WAN Yu-ping 2021 Hangzhou People's Procuratorate of Zhejiang Province, Hangzhou, Zhejiang 310012, China

Establishment Of Arapid Detection Method For Carbendazim Enzyme-Linked Immunosorbent, Jiang Wen-Hui, Wu Xiao-Sheng, Cui Na, Zhang Yu, Peng Zheng-Xue, Cui Hai-Feng, Wan Yu-Ping

Food and Machinery

Using the principle of enzyme-linked immunosorbent assay, a method for quantitative determination of carbendazim residues in fruits, vegetables, tea, and tobacco was established. First, the carbendazim hapten was prepared through acid-base neutralization chemical reaction, and then the anti-carbendazim monoclonal antibody was screened out by immunizing mice to prepare the enzyme-labeled goat anti-mouse anti-antibody, and finally the prepared anti-carbendazim Monoclonal antibodies and enzyme-labeled goat anti-mouse anti-antibodies were used in the development of enzyme-linked ELISA kits. The results showed that within the linear range of 0.1~8.1 μg/L, the target carbendazim had a good linear relationship, and the correlation coefficient (R2) …


Food Safety Supervision Based On Neural Network Technology In Large Data Environment, MENG Qing-jie, YAO Hai-chang 2021 Nanjing Vocational University of Industry Technology, Nanjing, Jiangsu 210046, China

Food Safety Supervision Based On Neural Network Technology In Large Data Environment, Meng Qing-Jie, Yao Hai-Chang

Food and Machinery

In the environment of large data, the application value of data mining and neural network technology in food safety supervision is discussed to provide ideas for the innovation of supervision mode in this field in China. Several important safety supervision models of Chinese traditional food first analyzed. Based on the analysis of the deficiencies of current supervision, the more mature supervision strategy of developed countries such as the United States is used to apply big data related technology to the food safety supervision, so as to make the data information timelier and more open. BP neural network is applied to …


Modeling And Simulation Of Bipolar Frequency Adjustable High Voltage Pulse Power Supply, WANG Zhuo, LI Zhen-mei, LI Hai-tao, LI Zong-zhe 2021 School of Electrical and Electronic Engineering, Shandong University of Technology, Zibo, Shandong 255049, China

Modeling And Simulation Of Bipolar Frequency Adjustable High Voltage Pulse Power Supply, Wang Zhuo, Li Zhen-Mei, Li Hai-Tao, Li Zong-Zhe

Food and Machinery

In order to solve the problem that the high-pressure sterilization system cannot remove multiple strains at the same time, this article uses a modular design to establish a sterilization system structure that eliminates three strains (Penicillium, yeast, Escherichia coli), and establishes a bipolar square wave and a high-frequency pulse with adjustable frequency Equivalent model of power supply and processing room, respectively. The system can output multiple bipolar square wave waveforms with different peak values when the frequency is 100~1 000 Hz with the pulse width of 10 μs, and can sterilize different pathogens separately. Simulation results show that the power …


Design And Test Of The Separator Of Kitchen Waste Residue Liquid Based On Edem Fluent Simulation, WANG Hao-xuan, GUO Lin, WANG Yi-ming, ZHANG Yong-hua, MAO Xin-bing, ZHANG Tian-hui 2021 College of Mechanical and Electrical Engineering, Yunnan Agricultural University, Kunming, Yunnan 650201,China

Design And Test Of The Separator Of Kitchen Waste Residue Liquid Based On Edem Fluent Simulation, Wang Hao-Xuan, Guo Lin, Wang Yi-Ming, Zhang Yong-Hua, Mao Xin-Bing, Zhang Tian-Hui

Food and Machinery

Aiming at the problems of large-scale food waste treatment production line, such as large capital investment, high cost of equipment operation and maintenance, and single operation form of small-scale food waste treatment equipment, based on SolidWorks, a kind of food waste treatment equipment, which integrates feeding, crushing, compression and dehydration, is designed to realize high integration, high automation, low energy consumption and low operation cost of food waste resource utilization Slag liquid separator. The particle model was established by EDEM software, and the particle motion parameters of kitchen waste were analyzed and optimized by RNG k—ε turbulence model in Fluent. …


Bacterial Composition Analysis Of Frozen Mussels Based On High-Throughput Sequencing, JIANG Hui-li, SHUI Shan-shan, WU Qiong-jing, CHEN Xiao-nan, ZHANG Bin, SU Lai-jin 2021 College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China

Bacterial Composition Analysis Of Frozen Mussels Based On High-Throughput Sequencing, Jiang Hui-Li, Shui Shan-Shan, Wu Qiong-Jing, Chen Xiao-Nan, Zhang Bin, Su Lai-Jin

Food and Machinery

After opening the shell with steam, the microorganisms on the surface of fresh mussel meat (FS), frozen mussel meat at -18 ℃ for 1 week (FS-1), frozen mussel meat for 3 weeks (FS-3) and frozen mussel meat for 9 weeks (FS-9) were extracted with DNA extraction kit and then analyzed by high-throughput sequencing. The effective sequences in all samples ranged from 31 896 to 42 812, and the operational taxonomic unit (OTU) ranged from 289 to 681. Among them, the OTU of FS-9 was the lowest, and the OTU of FS was the highest. Diversity index analysis showed that the …


Determination Of The Imaging System For Seedless White Grape Based On Image Evaluation, LI Ze-ping, LI Xue-lian, ZHOU Jun, GUO Jun-xian, HUANG Hua, GUO Yang, ZHANG Liang-liang 2021 College of Mechanical and Electronic Engineering, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China

Determination Of The Imaging System For Seedless White Grape Based On Image Evaluation, Li Ze-Ping, Li Xue-Lian, Zhou Jun, Guo Jun-Xian, Huang Hua, Guo Yang, Zhang Liang-Liang

Food and Machinery

In order to obtain the image of seedless white grape, the influence of background paper color, light source structure and lighting mode on the image was fully considered on the self-made acquisition platform. Firstly, the optimal level of each factor is determined by single factor test, then the acquisition platform is optimized by orthogonal test, and finally the optimal value level of each factor is determined by subjective and objective comprehensive evaluation image method. The results showed that the best values of the machine vision imaging system of seedless white grape were: the background paper is white, the light source …


Study On External Quality Detection Of Pepper Based On Machine Vision, REN Rui, ZHANG Shu-juan, ZHAO Hua-min, SUN Hai-xia, LI Cheng-ji, LIAN Meng-ru 2021 College of Engineering, Shanxi Agriculture University, Jingzhong, Shanxi 030800, China

Study On External Quality Detection Of Pepper Based On Machine Vision, Ren Rui, Zhang Shu-Juan, Zhao Hua-Min, Sun Hai-Xia, Li Cheng-Ji, Lian Meng-Ru

Food and Machinery

To realize the rapid detection of normal peppers and defective peppers (dark spots, insect marks and stripe skin), image acquisition and median filtering were used for denoising. 24 color feature variables and 20 texture feature variables of sample images were extracted. Threshold segmentation in the Otsu algorithm was used to extract two morphological feature variables. The Successive Projections Algorithm (SPA) was used to optimize 14 feature variables. Modeling was done based on the Least Squares Support Vector Machine (LS-SVM) method. The detection result of SPA-LS-SVM model is 98.67%, which is the best.


Optimization Of Preparation Technology And Antioxidant Activity Of Polysaccharide Sulfate From Pleurotus Ostreatus, LIANG Ying, BI Hong-mei, ZHENG Wen-feng, BI Hong-mei, LI Zhi-jiang 2021 College of Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Optimization Of Preparation Technology And Antioxidant Activity Of Polysaccharide Sulfate From Pleurotus Ostreatus, Liang Ying, Bi Hong-Mei, Zheng Wen-Feng, Bi Hong-Mei, Li Zhi-Jiang

Food and Machinery

Taking substitution rate as an index, the effects of concentration of sulfuric acid, reaction temperature and reaction time on sulfation of Pleurotus ostreatus polysaccharides were studied by concentrated sulfuric acid method. On the basis of single-factor results, the optimum preparation process of P. ostreatus polysaccharide sulfate was obtained by L9(34) orthogonal test. Secondly, the polysaccharide and sulfates of P. ostreatus polysaccharide were characterized by FT-IR spectrometer. Finally, the reductive power of VC and the scavenging rates of DPPH radical, superoxide anion radical and hydroxyl radical were determined to explore the antioxidant activity of P. ostreatus …


Extrusion Assisted Alkaline-Enzyme Extraction Process Optimization Of Protein Hydrolysate From Tie Guanyin Tea Residue And Its Functionalities, GUO Feng-xian, ZHENG Zong-ping, HUANG Cheng-fang, ZHAO Wei, YAO Yuan 2021 Quanzhou Normal University, Quanzhou, Fujian 362000, China

Extrusion Assisted Alkaline-Enzyme Extraction Process Optimization Of Protein Hydrolysate From Tie Guanyin Tea Residue And Its Functionalities, Guo Feng-Xian, Zheng Zong-Ping, Huang Cheng-Fang, Zhao Wei, Yao Yuan

Food and Machinery

Taking Tie Guanyin residues as the research object, through single factor experiment and response surface methodology, the effects of extrusion water content, sleeve temperature and screw frequency on its assisted alkaline-enzyme combined extraction of tea residue protein hydrolysates (TPH) were discussed, and the functionalities of TPH was then investigated. The optimal extrusion assisted extractions were water content of tea residue material 15%, sleeve temperature 97 ℃, screw frequency 40 Hz. Under the conditions the extraction rate of TPH was improved to (71.37±0.09)%, which was close to the predicted result. The results of TPH antioxidant activity showed the IC50 values …


Effect Of The Mixed Flour Of Chinese Yam, Coix Chinensis Tod. And Euryale Ferox On The Processing Quality Of Wheat Flour, SHI Jing-hong, HAO Shui-yuan, GUO Shu-wen 2021 Agronomy Inner Department of Hetao College, Bayannur, Inner Mongolia 015000, China

Effect Of The Mixed Flour Of Chinese Yam, Coix Chinensis Tod. And Euryale Ferox On The Processing Quality Of Wheat Flour, Shi Jing-Hong, Hao Shui-Yuan, Guo Shu-Wen

Food and Machinery

Chinese yam, Coix chinensis Tod. and Euryale ferox were added to wheat flour, the effect of mixed powder with different addition amount on the gelatinization characteristics, thermomechanical properties of dough and texture characteristics of steamed bread were analyzed by using rapid viscosity analyzer, Mixolab hybrid experiment instrument and texture analyzer. The results showed that as the amount of mixed powder added increased. The gelatinization viscosity, disintegration value, retrogradation value, gelatinization temperature, gelatinization time, dough water absorption, formation time and stability time of wheat flour gradually decreased, and cooking stability and protein weakening gradually increased. The hardness and chewiness of steamed …


Effects Of Ultrasound Combined Far-Infrared Radiation Drying On The Quality Of Banana Slices, SHI Xiao-wei, LIU Yun-hong 2021 College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China

Effects Of Ultrasound Combined Far-Infrared Radiation Drying On The Quality Of Banana Slices, Shi Xiao-Wei, Liu Yun-Hong

Food and Machinery

In order to investigate the quality characteristics of banana dried by ultrasound combined with far-infrared radiation drying, the banana slices were dried using ultrasound combined far-infrared radiation drying, and the drying was operated using the radiation temperature of 150, 210, 270 ℃ under the ultrasound power of 0, 30, 60 W. The effect of drying conditions on structure, rehydration ratio, shrinkage, color difference, total phenol content, VC content and antioxidant activity of banana slices were compared. The results showed that as the increases of radiation temperature and ultrasound power, the hardness values and shrinkage rate of banana slices decreased, and …


Purification And Characterization Of Metmyoglobin Reductase In Beef Longissimus Muscle, ZHAO Li-jun, ZHAO Ke, ZHAO Gai-ming, MA Yang-yang, ZHU Yao-di, LI Miao-yun, HUANG Ming-yuan 2021 College of Food Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, China

Purification And Characterization Of Metmyoglobin Reductase In Beef Longissimus Muscle, Zhao Li-Jun, Zhao Ke, Zhao Gai-Ming, Ma Yang-Yang, Zhu Yao-Di, Li Miao-Yun, Huang Ming-Yuan

Food and Machinery

The longissimus muscle from chilled beef was used as raw material to preliminary study the purification and characteristics of metmyoglobin reductase. The effect of temperature, pH and metal ions on the metmyoglobin reductase activity was investigated. Results revealed that the metmyoglobin reductase of longissimus muscle could be crudely purified with ammonium sulfate (65% saturation) and ultrafiltration (>50 kDa). SDS-PAGE analysis showed that the molecular weight of the metmyoglobin reductase of longissimus muscle was 29.0~66.4 kDa, mainly. The proper temperature of the metmyoglobin reductase of longissimus muscle was 40 ℃, with the proper pH 6.0. Cu2+ could inhibit the …


Effect Of Sports Drinks On Color Stability Of Different Light-Cured Composite Resins, ZHANG Wei 2021 Baotou Railway Vacational & Technical College, Baotou, Inner Mongolia 014060, China

Effect Of Sports Drinks On Color Stability Of Different Light-Cured Composite Resins, Zhang Wei

Food and Machinery

In this study, in order to evaluate the effect of three kinds of sports drinks on the color stability of light cured composite resin materials after 1 and 6 months of immersion, the reference color values (L*, a*, b*) of four kinds of resin composites were measured, and samples from each group of composite materials were randomly selected and immersed in sports drinks and distilled water for 1 month and 6 months. The color values of the samples after immersion were re measured, and the color change value (DE) was calculated. The data were …


Effects Of Threshing, Redrying With Collaborative Aging On Polyphenols Content In Tobacco-Planting Area Of Liangshan In Sichuan, MA Ya-ping, LI Li, LUO Cheng, LI Dong-liang 2021 Technology Center, China Tobacco Sichuan Industrial Co., Ltd., Chengdu, Sichuan 610066, China; Key Laboratory for Harmful Components and Tar Reduction in Cigarette of Sichuan Province, Chengdu, Sichuan 610066, China

Effects Of Threshing, Redrying With Collaborative Aging On Polyphenols Content In Tobacco-Planting Area Of Liangshan In Sichuan, Ma Ya-Ping, Li Li, Luo Cheng, Li Dong-Liang

Food and Machinery

Studied the changes of polyphenols including chlorogenic acid, neochlorogenic acid, 4-O-coffee-D-quinic acid, scopoletin, rutin, kaempferol glycoside and polyphenols content in Sichuan Liangshan flue-cured tobacco leaves under different key parameters of threshing and redrying (a1 was one wetting process outlet temperature; a2 was redrying zone 1 temperature~a5 was redrying zone 4 temperature) and alcoholization duration. The results show that: ① one wetting process outlet temperature have a certain positive correlation with polyphenols content, and redrying zone 1 temperature~ redrying zone 4 temperature are not significantly correlation. ② Chlorogenic acid, neochlorogenic acid, rutin, kaempferol glycoside and polyphenols content decrease with the increase …


Construction Of Lead (Ii) Ion Detection Method Based On Regulation Of Thiacyanine Self-Assembly By Nucleic Acid Aptamer, ZHAO Han, YU Li-jia, WU Zhi-sheng, ZHANG Ying-lin, ZHANG Hua-wei, SHI Xiao-dong 2021 National Center for Occupational Safety and Health, NHC, Beijing 102308, China

Construction Of Lead (Ii) Ion Detection Method Based On Regulation Of Thiacyanine Self-Assembly By Nucleic Acid Aptamer, Zhao Han, Yu Li-Jia, Wu Zhi-Sheng, Zhang Ying-Lin, Zhang Hua-Wei, Shi Xiao-Dong

Food and Machinery

In this paper, regulation of self-assembly properties of thiacyanine dye (Dye2) by nucleic acid aptamer (T30695) was used to construct a specific recognition unit for detecting Pb2+. G-quadruplexes were formed to interact with dye 2 monomers, which could disassemble dimers of Dye2 into monomer, in the presence of Pb2+. The absorption vales at 422 nm via 445 nm was used as a specific recognition signal to achieve the purpose of specificity and high sensitivity identification of Pb2+ only by UV-vis spectra. This system has a good linear relationship from 0.25 to 10.00 μmol/L (R2 …


Determination Of Icariin And Other 20 Functional Components In Food By Liquid Chromatography Tandem Mass Spectrometry, FAN Xiao-long, CHEN Ran, WU Wan-qin, LIU Guo-jiao, HUANG Kun, JIANG Feng 2021 Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430000, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430000, China

Determination Of Icariin And Other 20 Functional Components In Food By Liquid Chromatography Tandem Mass Spectrometry, Fan Xiao-Long, Chen Ran, Wu Wan-Qin, Liu Guo-Jiao, Huang Kun, Jiang Feng

Food and Machinery

A method for the quantitative determination of icariin, hypericin and Psoralen in mix liquor, beverage sample and tea substitutes were established by ultra-high-performance liquid chromatography tandem mass spectrometry. Samples were extracted by methanol water solution, diluted, purified, extracted, centrifuged, filtered, and determined by Thermo Acclain RSLC C18 (2.6 μm, 2.1 mm×100 mm) chromatographic column. The determination was carried out under the mode of electrospray positive and negative ion multi-reaction monitoring scanning (MRM) with acetonitrile and water. The maximum linear detection concentration of 20 compounds was 500 ng/mL, the correlation coefficient (R2) was greater than 0.99, and the …


Nondestructive Detection Of Pesticide Residues On Fruit Surface By Hyperspectral Imaging Technology, ZHANG Meng, JIA Shi-jie 2021 Electrical Engineering Branch, Jilin Railway Vocational and Technical College, Jilin, Jilin 132200, China

Nondestructive Detection Of Pesticide Residues On Fruit Surface By Hyperspectral Imaging Technology, Zhang Meng, Jia Shi-Jie

Food and Machinery

Based on the hyperspectral imaging technology, a non-destructive detection method for pesticide residues on fruit surfaces is proposed. By preprocessing the collected data and extract features, finding the optimal least squares support vector machine parameters through the bacterial population chemotaxis algorithm, a pesticide residue detection model was established, which was compared with the least squares support vector machine model to verify the superiority and accuracy the model. The results showed that the detection model had the highest detection accuracy based on the characteristic wavelength of the continuous projection method combined with the detection model, and its accuracy rate was 97.92%, …


Analysis And Design Of Bonito Cutting System At Low Ambient Temperature, LIANG Jing-kun, XIE Xin-gang, LI Jie-feng, YU Shu-chi 2021 Hainan Tropical Ocean University, Sanya, Hainan 052722, China

Analysis And Design Of Bonito Cutting System At Low Ambient Temperature, Liang Jing-Kun, Xie Xin-Gang, Li Jie-Feng, Yu Shu-Chi

Food and Machinery

To solve the problem that many temperature fluctuations occur in cold chain logistics from catching to selling Bonito, a new analog cutting system was designed under -18 ℃ environment based on wide-temperature PLC. Results showed that the design was superior to the traditional manual processing under room temperature and mechanical cutting after ultra-low temperature defrosting whether in low-temperature preservation or cutting speed, which reduced the cutting and exposure links and the temperature fluctuations effectively. After being assembled between the processing and quick-frozen cabins of the ocean-going fishing vessel, the synchronization of cutting and quick freezing could be realized, which could …


The Near Infrared Prediction Model Of K-Value Of Channel Catfish Fillets During Freeze-Thaw Cycles, QIAN Xiao-qing, ZHU Meng, SHI Gang-peng, XIONG Guang-quan, SHI Liu, WU Wen-jin, WANG Lan, WANG Shun-zhi, DING An-zi 2021 Hubei Agricultural Science and Technology Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Wuhan, Hubei 430064, China; School of Food and Biological Engineering, Hubei University of Technology, Wuhan, Hubei 430064, China

The Near Infrared Prediction Model Of K-Value Of Channel Catfish Fillets During Freeze-Thaw Cycles, Qian Xiao-Qing, Zhu Meng, Shi Gang-Peng, Xiong Guang-Quan, Shi Liu, Wu Wen-Jin, Wang Lan, Wang Shun-Zhi, Ding An-Zi

Food and Machinery

To determine the freshness change of Channel catfish fillets during freeze-thaw cycles and establishprediction model using near infrared spectroscopy, Near Infrared(NIR) reflectance spectroscopy was used to collect spectral data of Channel catfish meat during 5 times freeze-thaw processing, meanwhile the contents of ATP-related compounds of fish samples were obtained by HPLC (High Performance Liquid Chromatography) to calculate the freshness of K value, then the quantitative prediction model of K value was established based on the optimal wavelengths using PLS (Partial least squares regression) method and verified by t-test. The results showed that ATP content declined rapidly (P<0.05) from 2.15 to …


Evaluation And Classification For Physicohemical Quality Of Vine Tea On Entropy Method And Grey Relation Analysis, ZHANG Min, YU Ji, WANG Qi-yan, ZHANG Jin-cheng, YOU Xiang-tao, YAO Mao-jun, MA Cheng-jin 2021 Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie, Hunan 427000, China; Institute of Tea Science, Jishou University, Jishou, Hunan 416000, China

Evaluation And Classification For Physicohemical Quality Of Vine Tea On Entropy Method And Grey Relation Analysis, Zhang Min, Yu Ji, Wang Qi-Yan, Zhang Jin-Cheng, You Xiang-Tao, Yao Mao-Jun, Ma Cheng-Jin

Food and Machinery

Taking 10 kinds of vine tea samples collected as objects, 15 related physicohemical quality indexes of vine tea were measured. Four core indexes of tea infusioninflavonoids(A11), polyphenols(A12), soluble sugar(A13), and brightness(A5) were determined by variation analysis, correlation analysis, and principal component analysis. After that, the index weight was determined by the entropy method, and the grey correlation analysis was used to construct the evaluation model of the physicohemical quality: φimodelscore=ξA11×0.181 1+ξA12×0.224 2+ξA13×0.415 4+ξA3×0.179 3, and the fitting degree between the model score …


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