Fish-Spec: Fast Identification System For Handheld Spectroscopy And Species Classification,
2025
University of North Dakota
Fish-Spec: Fast Identification System For Handheld Spectroscopy And Species Classification, Mitchell Sueker, Nicholas Mackinnon, Gregory Bearman, Amanda Tabb, Diane Kim, Rosalee S. Hellberg, Alireza Akhbardeh, Hamid Reza Marateb, Jianwei Qin, Moon Kim, Fartash Vasefi, Hossein Kashani Zadeh
Food Science Faculty Articles and Research
Accurate fish species identification is critical to prevent mislabeling and fraud in the seafood industry. We present a handheld multi-mode point spectroscopy system that combines fluorescence (365 and 395 nm excitation) and reflectance measurements in the visible to near-infrared (∼350–900 nm) and short-wave infrared (∼900–1700 nm) regions for rapid, non-destructive classification of fish fillets. Tissue spectra were acquired at 25 positions on 68 fillets from 11 species, in both frozen and thawed states. Feature-level fusion across all four modes enabled higher classification accuracy than any single mode alone. A global machine-learning model classified all species with 85 ± 2.8 %, …
Prediction Model Of Storage Quality Change And Shelf Life Of Dried Pork Slice,
2025
School of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China
Prediction Model Of Storage Quality Change And Shelf Life Of Dried Pork Slice, Chen Xiaohe, Qian Ping, Huang Ning, Yang Ran, Qu Lingbo, Zhao Changcheng, Li Chunxia
Food and Machinery
[Objective] To investigate the quality changes of dried pork slices under different temperature storage conditions and to predict their shelf life. [Methods] Hardness, color parameters (L* value, a* value, b* value ), and sensory quality of dried pork slices were investigated under storage at 25, 35, 45, and 55℃. Pearson correlation analysis was performed for each index, and the key index was fitted using a dynamic model. Combining the Arrhenius equation with the Q10 model, a shelf life prediction model was established. [Results] Under storage at 25, 35, 45, and 55 ℃, the a* value of dried pork slices decreased …
Analysis Of Aroma Volatile Compounds In Black Tea Produced From The Large-Leaf Variety In Northern Guangxi Based On Hs-Spme-Gc-Ms,
2025
Tea Research Institute of Guangxi , Guilin 541004 , China;Guangxi Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization , Guilin 541004 , China;Guangxi Field Scientific Observation and Research Station for Tea Resources , Guilin 541004 , China
Analysis Of Aroma Volatile Compounds In Black Tea Produced From The Large-Leaf Variety In Northern Guangxi Based On Hs-Spme-Gc-Ms, Ting Chen, Chunyu Guo, Yingqi Wu, Min Li, Yuelan Pang, Yan Qing
Journal of Food Science and Biotechnology
[Objective ] This study explores the unique flavor characteristics and volatile compounds of black tea produced from the large-leaf variety in northern Guangxi,aiming to reveal the material basis of aroma formation of this variety.[Method ] Sensory evaluation,determination of biochemical parameters,headspace-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS ),and odor activity value (OAV ) analysis were performed in combination with chemometrics analysis to analyze the flavor characteristics and volatile compounds of the black tea.[Result ] The black tea samples produced from the large-leaf variety in northern Guangxi had similar aroma properties and were characterized by distinct floral and fruity aromas.A total of 88 volatile …
Advances In The Discovery Of Active Natural Products By Large Fragment Cloning,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China
Advances In The Discovery Of Active Natural Products By Large Fragment Cloning, Dandan Zeng, Jiayu Chen, Tianlu Mo
Journal of Food Science and Biotechnology
Active natural products have diverse structures and activities and can be a major source of small molecule drugs.Given the emergence of multi-drug resistant bacteria and superbacteria,there is an urgent need to develop new active natural products.Large fragment cloning has been an important tool for the discovery of active natural products in recent years,and it has demonstrated obvious advantages in obtaining the biosynthetic gene clusters of active natural products.This approach,combined with efficient cluster-targeted screening methods,has significantly accelerated the discovery of active natural products.This review summarizes a wide variety of active natural products.Furthermore,this paper elaborates on the principles and applications of several …
Preparation And Application Of A Polyclonal Antibody To Chlamydomonas Reinhardtii Rsp 10,
2025
College of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Preparation And Application Of A Polyclonal Antibody To Chlamydomonas Reinhardtii Rsp 10, Chan Lu, Yuanyuan Zhang, Ye Tao, Zhenchuan Fan, Yanxia Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the cilium assembly of the radial spoke protein RSP 10 and develop a highly specific polyclonal antibody to RSP 10.[Method ] Firstly,the prokaryotic expression vector pMal-C 2X-rsp10 was constructed,and the expression of MBP-labeled RSP 10 was induced in Escherichia coli BL21 (DE3).After the fusion protein was purified,and the MBP label was excised.The obtained protein was used to immunize New Zealand male white rabbits,and the antibody titer in the anti-serum was determined by indirect ELISA.[Result ] The rabbit anti- Chlamydomonas reinhardtii RSP 10 antibody with high specificity and high sensitivity was obtained after purification …
Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China;School of Pharmacy , Shanghai University of Medicine and Health Sciences , Shanghai 201318 , China
Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles, Yaqiong Chen, Yumeng Li, Hui Wu, Hongdong Song, Xiao Guan
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the dispersion and utilization of caffeic acid phenethyl ester (CAPE ) in aqueous solutions.[Method ] CAPE-loaded hordein (CAPE-HD ) nanoparticles were constructed via an anti-solvent precipitation method.The physicochemical properties of the nanoparticles were characterized,and the antioxidant properties of the nanoparticles within Caco- 2 cells were investigated.[Result ] The components within the CAPE-HD nanoparticles interacted through non-covalent bonds,such as hydrogen bonding,resulting in an altered state of CAPE upon nanoparticle formation.The CAPE-HD nanoparticles exhibited near-spherical morphology with an average particle size of 193.93 nm,an average polydispersity index (PDI) of 0.26,and average Zeta potential of 14.50 …
Bacterial Diversity In Milk And Traditionally Fermented Dairy Products In Balin Right Banner,
2025
School of Food Science and Engineering , Inner Mongolia Agricultural University , Hohhot 010018 , China
Bacterial Diversity In Milk And Traditionally Fermented Dairy Products In Balin Right Banner, Lijuan Liu, Gaowa Bai, Jinghe Yu, Fang Wang, Youyi Zhang, Li Zhu, Dalai Wuyun
Journal of Food Science and Biotechnology
[Objective ] This study explored the bacterial diversity in milk and traditional fermented dairy products in Balin Right Banner,aiming to lay a foundation for the development and utilization of bacterial resources in the milk and traditional fermented dairy products in this region.[Method ] A total of 32 samples of cow milk,milk tofu,and sour fermentation supernatant of milk tofu were collected from Balin Right Banner,and their bacterial diversity was analyzed by 16S rRNA high-throughput sequencing.[Result ] The diversity and abundance of bacterial communities varied among the milk and traditional fermented dairy products in Balin Right Banner.The dominant bacterial phyla were Firmicutes …
Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets, Sisi Li, Wei Zhou, Zhijin Zhou, Bin Wang, Chengjun Qiu, Zhangsheng Zhang, Qi Zhang, Yun Sun, Xu Xu, Tao Shen, Jianzhong Han, Daofeng Qu
Journal of Food Science and Biotechnology
[Objective ] To evaluate the antimicrobial resistance and transmission risk of foodborne Escherichia coli in wet markets,this study isolated E.coli from pork,aquatic products,vegetables,and wastewater collected from three wet markets in Hangzhou,Zhejiang.[Method ] The isolates were identified by 16S rRNA sequencing,and their antimicrobial resistance phenotypes were determined via the disk diffusion method.PCR was employed to detect antimicrobial resistance genes and integrons in the isolates.The antimicrobial resistance characteristics of E.coli were analyzed,and conjugation experiments were performed to assess the potential for horizontal transfer of antimicrobial resistance genes.Co-occurrence network analysis was conducted to evaluate the transmission risk of antimicrobial resistance among the isolates.[Result …
A Zif- 8 Electrode Immobilizing Enzyme For Detection Of Glucose Solution In Food,
2025
Key Laboratory of Chemistry and Chemical Engineering on Heavy-Carbon Resources , College of Chemistry and Chemical Engineering , Yili Normal University , Yining 835000 , China
A Zif- 8 Electrode Immobilizing Enzyme For Detection Of Glucose Solution In Food, Tingmei Ma, Shouxin Tang, Wenpan Shi, Yuchen Ma, Haijun Du
Journal of Food Science and Biotechnology
[Objective ] The aim was to construct an electrochemical sensor based on a metal-organic framework-modified electrode for the highly sensitive detection of glucose solution in beverages.[Method ] An electrochemical sensor based on ZIF- 8 composite carbon nanotubes and immobilizing glucose oxidase (GOx ) was prepared by in-situ synthesis and immobilized enzyme technology.The microstructure and morphology of the material were characterized by X-ray diffraction (XRD ),scanning electron microscopy (SEM ),UV-Vis spectroscopy,and infrared spectrometry (IR).The electrochemical performance of the electrode immobilizing enzyme was tested by electrochemical workstation.[Result ] The prepared electrode immobilizing enzyme showed excellent detection performance for glucose solution,with a limit …
Establishment Of A Molecularly Imprinted Electrochemical Sensor Nh 2-Mwcnts@ 4-Fpba For The Detection Of Epidermal Growth Factor In Dairy Products,
2025
College of Food Engineering and Nutritional Science , Shaanxi Normal University , Xi’an 710119 , China
Establishment Of A Molecularly Imprinted Electrochemical Sensor Nh 2-Mwcnts@ 4-Fpba For The Detection Of Epidermal Growth Factor In Dairy Products, Ruyue Zhang, Yiwei Wang, Hongxiu Zeng, Bini Wang, Fuxin Zhang, Zhenquan Huo, Yufang Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to develop a rapid and accurate method for detecting epidermal growth factor (EGF ).[Method ] 4-Formylphenylboronic acid (4-FPBA ) was covalently anchored to the surface of aminocarbon nanotubes (NH 2-MWCNTs ) to synthesize nanocomposites (NH 2-MWCNTs@ 4-FPBA ),and NH 2-MWCNTs@ 4-FPBA was modified on a glassy carbon electrode.When EGF was present,4-FPBA captured the EGF template through benzaldehyde,and then the electrode was immersed in pyrrole solution to electropolymerize to form a pyrrole molecularly imprinted layer.Finally,after dissociation and removal of the EGF template in an acidic solution,a three-dimensional cavity complementary to the EGF template was formed.[Result ] …
Comparison Of The Bioactivities Of Ziziphus Jujuba Polysaccharide And Its Degradation Products,
2025
College of Bioscience and Engineering , Hebei University of Economics and Business , Shijiazhuang 050061 , China
Comparison Of The Bioactivities Of Ziziphus Jujuba Polysaccharide And Its Degradation Products, Chunling Zhou, Xiaoyan Huang, Xuan Zheng, Ju Zhang
Journal of Food Science and Biotechnology
[Objective ] This study aims to compare Ziziphus jujuba polysaccharide (ZJP) and its degradation products in terms of their chemical composition and bioactivities.[Method ] ZJP was degraded by α-L-rhamnosidase,cellulase,snail enzyme,and trifluoroacetic acid to prepare different degradation products (ZJP-R,ZJP-C,ZJP-S,and ZJP-T ),and the chemical composition and degradation effects were compared.On the basis of in vitro antioxidant and probiotic activities,the in vivo antioxidant,anti-aging,anti-stress,and neuroprotective activities of ZJP and its degradation products were compared with Caenorhabditis elegans as a model system.[Result ] The mass fraction of reducing sugar and average degree of polymerization of ZJP showed significant variations among different degradation treatments.In addition,Sephadex G- …
Immunomodulatory Effects And Marker Components Of Alcohol Extract From Acanthopanax Senticosus (Rupr . Et Maxim .) Harms- Ganoderma Applanatum (Pers.) Pat.,
2025
School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Immunomodulatory Effects And Marker Components Of Alcohol Extract From Acanthopanax Senticosus (Rupr . Et Maxim .) Harms- Ganoderma Applanatum (Pers.) Pat., Lihong Wang, Kairui Sheng, Ming Lu, Jin Su, Yang Ping, Yu Zhang, Ji Zhao, Yixia Gong
Journal of Food Science and Biotechnology
[Objective ] The aim of this study was to analyze the compositional changes of the alcohol extracts of Acanthopanax senticosus (Rupr.et Maxim.) Harms (APS )-Ganoderma applanatum (Pers.) Pat.(GA) before and after compounding at a mass ratio of 5∶1 (mAPS∶mGA=5∶1) and to investigate the immunomodulatory effects and marker components of the extracts.[Method ] The mouse models of compromised immunity and delayed-type hypersensitivity (DTH ) were established with cytoxan (CTX ) and 2,4-dinitrochlorobenzene (DNCB ),respectively,to investigate the effects of the alcohol extracts on the immune system.Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS ) was used to analyze the changes of the active ingredients …
Effects And Differences Of (+)-Catechin And (-)-Epicatechin On Antioxidant Activity Of Hek 293t Cells,
2025
Institute of Agro-food Technology , Jilin Academy of Agricultural Sciences , Changchun 130033 , China
Effects And Differences Of (+)-Catechin And (-)-Epicatechin On Antioxidant Activity Of Hek 293t Cells, Chang Sun, Ning Xia, Chao Wang, Fengxian Qin, Chengyu Lyu, Jian Liu, Tiezhu Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects and differences of the natural antioxidant (+)-catechin and its isomer (-)-epicatechin on the antioxidant activity of HEK 293T cells based on the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE ) pathway.[Method ] The effects of (+)-catechin and (-)-epicatechin on the viability of HEK 293T cells were investigated via the methyl thiazolyl tetrazolium (MTT ) assay.Reporter genes were employed to investigate the regulatory effects on the activation of ARE.The effects of (+)-catechin and (-)-epicatechin on the mRNA and proteins levels of the downstream factors [glutamate-cysteine ligase modifier subunit (GCLM …
Effects Of Α-Amylase Addition On The Characteristics Of Infant Rice Flour,
2025
Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , China
Effects Of Α-Amylase Addition On The Characteristics Of Infant Rice Flour, Huihui Liu, Zhiyong Dai, Mengyi Li, Jiao Peng, Xing Meng, Luyan Liao, Rongbin Zhang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of α-amylase addition on the characteristics of infant rice flour.[Method ] Infant rice flour samples with different α-amylase addition were prepared.The basic composition,relative molecular mass of starch,gelatinization properties,hydration properties,texture properties,in vitro digestion characteristics,and microstructure of infant rice flour were determined.Furthermore,the correlations between the physicochemical characteristics and quality characteristics of infant rice flour were analyzed.[Result ] The weight-averaged molecular weight and the number-averaged molecular weight of starch in infant rice flour decreased with the increase in α-amylase addition.The gelatinization curve of infant rice flour with α-amylase addition was relatively gentle,and the gelatinization …
Effects Of Spices On Microbial Community Structure And Volatile Flavor Components Of Fuling Pickled Mustard Tuber,
2025
Chongqing Yudongnan Academy of Agricultural Sciences , Chongqing 408000 , China
Effects Of Spices On Microbial Community Structure And Volatile Flavor Components Of Fuling Pickled Mustard Tuber, Ting Ren, Yuling Liu, Zhiping Zhao, Xiaoyong Chen, Yumei Peng, Hong Tao, Yuanli Luo
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of spices on the microbial community structure and volatile flavor compounds of Fuling pickled mustard tuber and explore the correlations between dominant microbial genera and volatile components.[Method ] The effects of spices on microbial community structure and volatile flavor components of Fuling pickled mustard tuber were investigated by high-throughput sequencing and headspace solid-phase microextraction (HS-SPME ) coupled with gas chromatography-mass spectrometry (GC-MS ).Pearson correlation coefficients were calculated to reveal the correlations between dominant microbial genera and volatile components.The mass fractions of total acids,nitrite,and reducing sugar were determined.[Result ] The dominant microbial …
Effects Of Shaping And Baking Processes On The Antioxidant Activity Of Round Roasted Green Tea,
2025
Hangzhou Tea Research Institute , CHINA COOP , Hangzhou 310016 , China;Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resources , Hangzhou 310016 , China
Effects Of Shaping And Baking Processes On The Antioxidant Activity Of Round Roasted Green Tea, Jun Liu, Gong Feng, Rong Tan, Weihong Huang
Journal of Food Science and Biotechnology
[Objective ] This study investigated the effects of shaping and baking processes on the antioxidant activity of round roasted green tea,aiming to provide theoretical support and practical reference for improving the quality and optimizing the processing techniques of green tea.[Method ] The influences of different processing stages and key procedures such as shaping and baking on the antioxidant activity of round roasted green tea were studied.A zebrafish model (in vivo ) and multiple indexes including DPPH radical scavenging rate,ABTS ⁺ radical scavenging rate,and ferric ion reducing antioxidant power (FRAP ) were employed for comprehensive evaluation of the antioxidant activity.[Result ] …
Effects And Mechanisms Of Phytosterol-Oil Bodies Emulsion On Lipid Metabolism Of Caenorhabditis Elegans,
2025
School of Life Sciences and Engineering , Henan University of Urban Construction , Pingdingshan 467036 , China
Effects And Mechanisms Of Phytosterol-Oil Bodies Emulsion On Lipid Metabolism Of Caenorhabditis Elegans, Jie Sun, Mengyuan Yuan, Yifei Liu, Fuyao Zhang, Zhuofan Yang, Guoyou Yin
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the lipid-lowering effects of the phytosterol-oil bodies emulsion (POE ).[Method ] Caenorhabditis elegans was taken as a model to evaluate the effects of POE on lipid metabolism,physiological indexes,and expression of genes associated with oxidative stress and fat metabolism.[Result ] Compared with the control group,different mass concentrations of POE reduced the mass fraction of fat (P<0.01) and the relative content of triglycerides (P<0.05),without toxicity to the growth and development of C.elegans (P<0.01).At the same time,it significantly reduced the level of reactive oxygen species and increased the levels of antioxidant enzymes.In addition,POE affected the expression levels of genes related to fat metabolism.qPCR results showed that POE up-regulated the mRNA level of sbp-1,while down-regulating the mRNA levels of fat-2,nhr-80,and fat-5 (P<0.05).[Conclusion ] POE may improve lipid metabolism by slowing fat deposition,regulating the fat peroxidation pathway,and alleviating oxidative stress in C.elegans.
Optimization Of Processing Technology And Analysis Of Volatile Components In Jinxuan Black Tea Cream,
2025
Guangxi South Subtropical Agricultural Sciences Research Institute , Chongzuo 532200 , China
Optimization Of Processing Technology And Analysis Of Volatile Components In Jinxuan Black Tea Cream, Yunxian Wang, Yunxiong Zhao, Lianfeng Luo, Baogui Liu, Jingjing Yang
Journal of Food Science and Biotechnology
[Objective ] This study is intended to optimize the preparation process of Jinxuan black tea cream,enhance the content of tea polyphenols,and compare the effects of different processing methods on the volatile components of the tea cream.[Method ] With dried Jinxuan black tea as the raw material,response surface methodology was adopted to examine the impacts of tea-to-water ratio,enzyme dosage,ultrasonic power,and ultrasonic duration on the mass fraction of tea polyphenols in the black tea cream.Furthermore,headspace solid-phase microextraction (HS-SPME ) coupled with gas chromatography-mass spectrometry (GC-MS ) was employed to detect and analyze the volatile components in the black tea cream samples processed …
Optimization Of The Fermentation Medium Of Aneurinibacillus Jxn- 1 For Caproic Acid Production,
2025
Department of Brewing Engineering , Moutai Institute , Renhuai 564500 , China
Optimization Of The Fermentation Medium Of Aneurinibacillus Jxn- 1 For Caproic Acid Production, Guangdong Gan, Chunlin Zhang, Ting Huang, Junjun He, Chun Pu, Wei Shi, Mingye Peng, Tengfei Zhao
Journal of Food Science and Biotechnology
[Objective ] This study aims to increase the caproic acid yield of Aneurinibacillus JXN- 1.[Method ] First,a Plackett-Burman design was employed to screen the significant factors affecting caproic acid yield from eight medium components.Subsequently,the path of steepest ascent method was used to determine the optimal concentration ranges of these significant factors.Finally,a Box-Behnken design was utilized to establish a quadratic polynomial regression model,and the interactions among the significant factors were analyzed to predict the optimal medium composition.[Result ] The Plackett-Burman design identified the mass concentration s of sodium acetate and calcium folinate and the volume fraction of ethanol as significant influencing …
Research Progress In Light Treatment For Postharvest Preservation Of Fruits And Vegetables,
2025
School of Artificial Intelligence , Xiamen City University , Xiamen 361008 , China
Research Progress In Light Treatment For Postharvest Preservation Of Fruits And Vegetables, Lili Zheng, Yue Lin
Journal of Food Science and Biotechnology
Postharvest fruits and vegetables often face issues of senescence and diseases,which lead to a decline in quality.Although conventional preservation techniques are widely applied,some of them have certain health risks.Light treatment,as an emerging non-thermal and non-toxic preservation technology,has attracted attention in the food industry.On the basis of the impacts of light on plant physiology and microbial growth,light treatment helps to maintain the freshness and market value of fruits and vegetables.The light sources for light treatment mainly include light-emitting diodes (LED ),ultraviolet (UV),fluorescent lamps (FL),and pulsed light (PL).This review explores the application mechanisms and effects of light treatment in the preservation of …
