Construction And Antibacterial Activity Evaluation Of A Lipopeptide Delivery System,
2026
Sichuan Baoning Vinegar Co ., Ltd., Langzhong 637400 , China
Construction And Antibacterial Activity Evaluation Of A Lipopeptide Delivery System, Rong Chen, Mingye Guo, Ge Hou, Yan Chen
Journal of Food Science and Biotechnology
[Objective ] This study constructed a stable lipopeptide (LP) delivery system to improve the stability and dispersibility of LP,thereby enhancing their antibacterial efficacy.[Method ] LP particles were constructed with chitosan (CS) and sodium alginate (SA).Assembly property changes were evaluated by means of different construction and single factor experiments.Interactions among CS,LP,and SA were investigated by Fourier transform-infrared spectroscopy (FT-IR ),and the rheological properties of the system were examined by a rheometer.Antibacterial activity of LP particles was evaluated with Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus ) as model microorganisms.[Result ] LP particles prepared by dripping CS with the relative molecular mass …
Effects Of Modification Methods On The Processing Properties Of Cyperus Esculentus Powder,
2026
College of Food and Bioengineering , Qiqihar University , Qiqihar 161006 , China
Effects Of Modification Methods On The Processing Properties Of Cyperus Esculentus Powder, Xiaowei Sun, Chunli Song, Jian Ren
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the effects of three modification methods on the processing properties of Cyperus esculentus powder.[Method ] Three modification methods (superfine grinding,microwave treatment,and enzymatic treatment ) were employed to process Cyperus esculentus powder.The effects of these treatments on the processing properties including oil holding capacity,hydration properties,textural properties,and rheological properties of Cyperus esculentus powder were analyzed.[Result ] In comparison to the native Cyperus esculentus powder,ultrafine grinding treatment increased the specific surface area of the powder,enhanced the oil holding capacity by 74.57%,and significantly increased the loss angle (tan δ).Additionally,this treatment significantly increased the hardness,cohesiveness,and chewiness of the …
Effects Of Compound Protease Fermentation On The Industrial-Scale Production Of Very High Gravity Brewing Beer,
2025
Tsingtao Brewery Co . Ltd., Qingdao 266237 , China
Effects Of Compound Protease Fermentation On The Industrial-Scale Production Of Very High Gravity Brewing Beer, Zongming Chang, Guangfeng Liu, Li Fang, Jiajie Zhang, Hua Yin
Journal of Food Science and Biotechnology
[Objective ] This study aims to address the technical problems of insufficient nitrogen content and an imbalanced amino acid composition in very high gravity wort produced with high-level syrup adjuncts.[Method ] This study investigated the effect of protease-assisted saccharification on wort protein composition.Then,a comprehensive evaluation was conducted on the brewing performance during 22 ° P very high gravity beer production.[Result ] During the proteolysis stage of saccharification,a synergistic addition of neutral protease and flavourzyme protease at a mass ratio of 6∶4 enabled efficient directed hydrolysis of malt proteins.In the wort,the α-amino nitrogen mass concentration was increased by 30%,while the mass …
Rapid Detection Of Aflatoxin B 1 Contamination Levels In Peanuts Based On Dual-Band Hyperspectral Imaging And Machine Learning,
2025
The College of Medical Technology , Shanghai University of Medicine & Health Sciences , Shanghai 201318 , China
Rapid Detection Of Aflatoxin B 1 Contamination Levels In Peanuts Based On Dual-Band Hyperspectral Imaging And Machine Learning, Lu Ren, Shuyue Shen, Wanrong Shi, Chuang Rui, Maofeng Dong, Tingting Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to achieve rapid detection of AFB 1 contamination levels in peanuts through dual-band hyperspectral imaging and machine learning.[Method ] A peanut AFB 1 contamination level warning model was constructed via a dual-band hyperspectral imaging system consisting of visible near-infrared spectroscopy at 400~1 000 nm and short-wave near-infrared spectroscopy at 900~1 700 nm,combined with machine learning.Peanut samples with four contamination levels were collected,in which the mass fraction of AFB 1 was determined by ultra-high performance liquid chromatography-tandem mass spectrometry.The effects of four pretreatment methods [first derivative,second derivative,multiplicative scatter correction,and standard normal variate (SNV )],two characteristic wavelength …
Effects Of Aerated Storage On The Microbial Community And Functional Genes Of Pickles,
2025
Institute of Eastern Himalaya Research , Dali University , Dali 671003 , China
Effects Of Aerated Storage On The Microbial Community And Functional Genes Of Pickles, Hengye Jing, Wei Deng, Fuliang Qi, Kun Tan, Rong She, Xiaoyan Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of aeration on the microbial community structure and metabolic functional genes in pickles.[Method ] Amplicon sequencing was carried out for the 16S rRNA and ITS rRNA gene fragments from three groups (aerated group,sealed group,and the initial group ) within the same pickle system.The EasyAmplicon pipeline and PICRUSt 2 were employed to assess bacterial and fungal community diversity,composition,and functional gene variations.[Result ] Compared with the initial group,the sealed group showed only minor changes in microbial diversity,whereas the aerated group exhibited significant microbial divergence from the initial group.After aeration,the number of rare taxa …
Protective Effects Of Ovalbumin-Sodium Carboxymethylcellulose Colloidal Particles On Fish Oil Emulsions,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Protective Effects Of Ovalbumin-Sodium Carboxymethylcellulose Colloidal Particles On Fish Oil Emulsions, Weichun Pan, Ya Wen, Huabin Liao, Feina Gu, Qiuping Zhang, Fuge Niu
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the impact of curcumin-loaded ovalbumin (OVA )-carboxymethylcellulose (CMC ) (OVA -CMC-Cur ) colloidal particles on the stability of fish oil emulsions.[Method ] The OVA -CMC-Cur colloidal particles were prepared with OVA and CMC at varying mass ratios and pH.The colloidal particles were then used as the stabilizer to prepare fish oil emulsions.The structural characteristics,stability,and digestive behaviors of the emulsions were investigated.[Result ] At the OVA -CMC mass ratio of 2∶1,the OVA -CMC-Cur particle-stabilized fish oil emulsions exhibited smaller particle sizes.These particles formed a dense,compact interfacial film at the oil-water interface,resulting in more uniform …
Research Progress In The Regulation Of Intestinal Stem Cell Homeostasis By Dietary Patterns,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Research Progress In The Regulation Of Intestinal Stem Cell Homeostasis By Dietary Patterns, Haichao Zhang, Qi Sun, Yumei Qin, Yanpei Gu, Jianzhong Han
Journal of Food Science and Biotechnology
Intestinal stem cells (ISCs ) located at the bottom of intestinal crypts are the key factors driving the repair and rapid renewal of the intestinal epithelium.Their proliferation and differentiation are finely regulated by the microenvironment of the niche.ISCs,as dietary nutrient sensors,can respond to different dietary signals to regulate intestinal homeostasis.The author systematically summarizes the main signaling pathways involved in the regulation of ISCs and reviews the effects and functioning mechanisms of dietary patterns such as high-fat diets,intermittent fasting,calorie restriction,and ketogenic diets on the homeostasis of ISCs,aiming to provide a theoretical basis for the development and application of dietary intervention strategies …
Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia,
2025
Food Laboratory of Zhongyuan , Luohe 462300 , China
Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia, Feng Li, Jiawei Huang, Xiaowen Guo, Lulu Jiang, Hongyan Zhao, Qiang Li, Chenxiang Sun, Shuying Feng, Qianqian Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the ameliorative effects of a Lilium brownii -Plantago asiatica L.compound solid beverage on hyperuricemia and to elucidate its potential mechanisms.[Method ] Network pharmacology was employed to screen the active constituents,potential targets,and related pathways.Key targets were identified based on a protein-protein interaction (PPI) network.Molecular docking was conducted between the key proteins and active constituents.An animal experiment was performed to validate the effects of the compound solid beverage on serum uric acid levels,renal function indices,activities of uric acid-producing enzymes,and the levels of renal urate transporters.[Result ] Network pharmacology analysis indicated that Lilium brownii and Plantago …
Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp.,
2025
Jiangsu Zhongnongke Food Engineering Co ., Ltd ., Suqian 223800 , China
Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp., Jing Zhang, Mengmeng Xu, Liting Zhao, Cui Li, Lei Chen, Song Ming, Zhongyang Ding
Journal of Food Science and Biotechnology
Zinc and selenium are essential trace elements for maintaining human normal physiological function.Deficiency of zinc or selenium can result in immune dysfunction,chronic diseases such as cardiovascular diseases and hyperlipidemia,and even increase the risk of cancer.The human body cannot independently synthesize these trace elements,which can only be obtained from the daily diet.However,natural food sources have low content of zinc and selenium,which is insufficient to meet the bod y’s needs.Therefore,developing fortified foods with trace nutrients is an important approach to meeting residents ’ requirements.Edible fungi serve as natural carriers for enriching trace elements and ideal dietary supplements.Pleurotus spp.,as a typical example,not only …
Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China ;Key Laboratory of Industrial Biotechnology of Ministry of Education , Jiangnan University , Wuxi 214122 , China
Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu
Journal of Food Science and Biotechnology
[Objective ] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method ] Through the analysis of protein expression,enzymatic properties,and whole-cell catalysis results,tyrosine decarboxylase (TDC ) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis.Subsequently,tyramine oxidase (TYO ) was introduced to construct the TDC-TYO pathway for tyrosol production.Meanwhile,the LAAD-PDC pathway for tyrosol synthesis was established.A dual-enzyme co-expression system was established,and the effects of tandem enzyme order,production pathways,and key enzyme sources on tyrosol yield were analyzed.The recombinant strain B 1,carrying the plasmid pET 28a-EfTDC-TYO,was obtained through whole-cell …
Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy,
2025
Key Laboratory of Industrial Biotechnology of the Ministry of Education , Jiangnan University , Wuxi 214122 , China;Yixing Institute of Food and Biotechnology Co ., Ltd., Wuxi 214200 , China
Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy, Kai Wang, Xuewei Pan, Taowei Yang, Zhiming Rao
Journal of Food Science and Biotechnology
[Objective ] The production of the aromatic compound salicylic acid (SA) is limited by its inhibitory effects on enzymes related to cellular energy metabolism.This study aims to develop a strategy for avoiding the impact of SA on enzymes related to cellular energy metabolism and increase the yield of SA.[Method ] A highly efficient Escherichia coli strain was designed to improve SA production by optimizing metabolic pathways and enzyme systems.[Result ] The genome-scale metabolic model GEMS 1515 was employed to predict the key bottle necks in the phosphoenolpyruvic acid (PEP) pathway and SA biosynthesis pathways.The SA yield was successfully increased from …
Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum,
2025
College of Biotechnology and Pharmaceutical Engineering , Nanjing Tech University , Nanjing 211816 , China ;Nanjing Hi-Tech Biological Technology Research Institute Co ., Ltd., Nanjing 210032 , China
Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum, Qingguo Liu, Hanjie Ying, Wenjun Sun
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the feasibility of the fermentation process for L-lysine production by immobilized Corynebacterium glutamicum.[Method ] Firstly,the effects of different volume fractions of seed medium on the fermentation stability of immobilized Corynebacterium glutamicum were examined.Subsequently,the impacts of immobilization carriers with different specifications on the fermentation performance were analyzed.Finally,a comparative analysis was conducted on the differences between the fermentation processes with free and immobilized Corynebacterium glutamicum.[Result ] The addition of 5% volume fraction of seed medium adequately sustained the growth and metabolic activities of Corynebacterium glutamicum.Higher volume fractions diverted carbon sources towards bacterial growth,thereby limiting product …
Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast,
2025
College of Chemistry and Bioengineering , Guilin University of Technology , Guilin 541004 , China
Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast, Huixiang Zhang, Nan Lin, Jiaru Wu, Xin Cheng, Weiwu Liang, Shijun Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to breed a fruit wine yeast strain with low methanol production by protoplast fusion.[Method ] With yeast strains LAB and XR as parents,protoplast fusion was employed to breed the yeast strains for fruit wine fermentation with a low mass concentration methanol and a high volume fraction ethanol.The process of yeast protoplast preparation was optimized by single factor and orthogonal experiments with the formation rate,regeneration rate,and preparation rate of protoplasts as indicators.The parental protoplasts were inactivated by ultraviolet radiation and heating,and 35 g/dL PEG- 6000 was used for chemical fusion after inactivation.[Result ] The optimum conditions …
Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes,
2025
College of Enology , Northwest A&F University , Yangling 712100 , China
Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes, Jiayan Qu, Huiping Niu, Bowen Li, Xiao Wang, Lujun Wang, Zhumei Xi, Xuefei Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of different treatments on the postharvest preservation of grapes.[Method ] The impacts of 0.4 mg/L 24-epibrassinolide (EBR ),1×109 CFU/mL Metschnikowia pulcherrima,and their combination (YBR ) on postharvest storage of ‘Shine Muscat ’ grapes were evaluated.[Result ] Compared with the control (CK) group,EBR,Metschnikowia pulcherrima,and YBR treatments effectively reduced the water loss rate,maintained the firmness,and delayed the declines in protopectin and stem chlorophyll content of grape.In the later storage stage,YBR treatment delayed the decrease in flavonoid mass fraction,which was 12.95% higher than that of CK group.At the same time,YBR treatment reduced the mass …
Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat,
2025
College of Life Sciences , Linyi University , Linyi 276005 , China;Department of Biotechnology , The University of Suwon , Suwon 18323 , South Korea
Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat, Yanzhen Zhang, Ning Liu, Wei Wang, Xianqi Yao, Laixue Ni, Yunguo Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the feasibility of using the rapid test strip method for detecting key microbial quality control indicators in chilled meat,specifically the total plate count and Escherichia coli /coliforms.[Method ] The performance of the rapid test strip method was assessed through repeatability tests,and the national standard plate count method was used as the reference for method comparison.Statistical significance of the quantification results was analyzed by a t-test,and linear regression was adopted to analyze the correlation.[Result ] The repeatability deviation of the rapid test strip method (r≤0.25) met the requirements of ISO 4833—1:2013.No significant difference was …
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey,
2025
School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China ;Guizhou Xijiu Investment Holding Group Co ., Ltd ., Zunyi 564662 , China
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the impacts of single strain fermentation and mixed fermentation on the flavor,composition,and antioxidant activity of soybean whey.[Method ] Gas chromatography-ion mobility spectrometry (GC-IMS ) was coupled with E-nose and high-performance liquid chromatography (HPLC ) to a nalyze the flavor and function of soybean whey fermented by the single and mixed strains.[Result ] Compared with single-strain fermentation by Limosilactobacillus fermentum HJS- 1 or Kluyveromyces marxianus HJS- 2,mixed fermentation (Limosilactobacillus fermentum HJS- 1 + Kluyveromyces marxianus HJS- 2) effectively improved the levels of functional substances and antioxidant activity of soybean whey.Compared with non-fermented soybean whey,mixed …
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli,
2025
State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong
Journal of Food Science and Biotechnology
[Objective ] This study aimed to improve the yield and conversion rate of 5-methylpyrazine- 2-carboxylic acid (MPCA ) through biocatalysis.[Method ] Escherichia coli (E.coli）was used as the model organism to construct an MPCA synthesis system,and the whole-cell catalytic reaction conditions of recombinant E.coli were optimized.In addition,the parental host strain was subjected to ultraviolet mutagenesis for the screening of host strains tolerant to high mass concentrations of 2,5-dimethylpyrazine (DMP ).[Result ] The recombinant mutagenized strain could completely convert 10 g/L DMP to MPCA within 12 h in a 10 mL reaction system,achieving a yield of 12.9 g/L and a corresponding molar …
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation,
2025
School of Food Engineering , Harbin University , Harbin 150086 , China
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein,
2025
College of Life Sciences and Medicine , Zhejiang Sci-Tech University , Hangzhou 310018 , China;Shaoxing Academy of Biomedicine , Zhejiang Sci-Tech University , Shaoxing 312369 , China
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein, Zhuo Jiang, Weina Lu, Qi Lin, Zhan Luo, Zhengbin Lyu, Zuoming Nie
Journal of Food Science and Biotechnology
[Objective ] This study aims to design a fusion protein with high activities and high stability of superoxide dismutase (SOD ) and catalase (CAT ).[Method ] Using AcSOD 11 and FeCAT proteins as the research subjects,three fusion proteins of SOD and CAT were designed:AcSOD 11-(GGGGS )*3-FeCAT,FeCAT-(GGGGS )*3-AcSOD 11,and FeCAT- (EAAAK )*3-AcSOD 11,which were named AcSOD 11-G3-FeCAT,FeCAT-G3-AcSOD 11,and FeCAT-E 3-AcSOD 11,respectively.Soluble expression of these three fusion proteins was achieved in Escherichia coli BL21(DE3).The thermal stability and pH stability of the three fusion proteins were then assessed.[Result ] The three fusion proteins showed no significant difference in the specific activity of SOD,while …
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies,
2025
University of Nebraska-Lincoln
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies, Xu Shi
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Heart failure (HF) is a leading cause of morbidity and mortality worldwide, placing substantial clinical and economic burdens on society. Although advances in therapy have improved patient outcomes, the biological mechanisms that drive HF onset and progression remain incompletely understood. Increasing evidence suggests that the gut microbiota, the trillions of microorganisms residing in the gastrointestinal tract, plays a key role in some forms of heart disease, especially cardiovascular disease. Moreover, gut microbial dysbiosis has been associated with systemic inflammation, metabolic imbalance, and immune dysregulation, all of which contribute to the pathophysiology of HF. Cardiomyopathy, a primary disorder of the heart …
