Characteristics Of L-Malic Acid And Its Application In Orange Juice,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Characteristics Of L-Malic Acid And Its Application In Orange Juice, Xingyuan Liu, Yi Fan, Jiabin Bao, Qing Xing, Ruijin Yang, Yanjun Tong
Journal of Food Science and Biotechnology
[Objective ] The basic characteristics of L-malic acid and the application of L-malic acid in orange juice were studied.[Method ] The basic characteristic differences of L-malic acid,citric acid,and DL-malic acid were compared in terms of thermal stability,pH stability,and taste quality changes of electronic tongue.Subsequently,the three sour agent were added into orange juice.The effects of L-malic acid on the stability and volatile flavor of orange juice were analyzed based on the differences in centrifugal sedimentation rate,pH,electrical conductivity,particle size,Zeta potential,and volatile components.[Result ] L-Malic acid had good stability in the ranges of 40~121 ℃ and pH 2.0~7.0.L-Malic acid differed markedly from citric …
Structural Analysis And Functional Prediction Of Prgr , A Target Protein Of The Poria Ergosta- 7,22e-Dien- 3Β-Ol,
2025
Guizhou Light Industry Polytechnic University , Guian 561116 , China
Structural Analysis And Functional Prediction Of Prgr , A Target Protein Of The Poria Ergosta- 7,22e-Dien- 3Β-Ol, Yongfei Liu, Yanling Ren, Tao Wang, Maozhao Wu, Jing Peng, Changyun Wu, Zexiu Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the mechanism of the interaction between ergosta- 7,22E-dien- 3β-ol,an active sterol of Poria,and progesterone receptor (PRGR ) and the potential therapeutic effects via bioinformatics and molecular docking.[Method ] The TCMSP database was used to screen the active ingredients of Poria,and MEGA X (10.0.5) was used for alignment of multiple PRGR sequences.The primary structure of PRGR was analyzed by ProtParam,and the hydrophobicity of PRGR was predicted by Protscale.The amino acid sequence of PRGR was analyzed by Prosite,and the secondary structure of PRGR was analyzed on [email protected] RCSB PDB database was used to predict the …
Advances In Application Of Steam Explosion Technology In Edible Mushroom Industry,
2025
College of Life Sciences , Fujian Agriculture and Forestry University , Fuzhou 350002 , China;Institute of Food Science and Technology , Fujian Academy of Agricultural Sciences , Fuzhou 350003 , China
Advances In Application Of Steam Explosion Technology In Edible Mushroom Industry, Jie Yang, Na Zheng, Chunmei Lai, Mingyi Liu, Shibo Li, Shanshan Wei, Pufu Lai, Zheng Xiao
Journal of Food Science and Biotechnology
Steam explosion technology,an emerging physical pretreatment method,is increasingly applied in the edible mushroom industry.This technology subjects materials to instantaneous explosion under high-temperature and high-pressure steam,significantly improving their physical structures and chemical composition.It exhibits unique strengths in modifying edible mushroom cultivation substrates,extracting active compounds,and utilizing by-products as resources. This provides a new technical path for the high-quality development of the edible mushroom industry.The author primarily summarizes the equipment,principles,and influencing factors of steam explosion technology,as well as its diverse roles in modifying cultivation substrates,extracting active substances from edible mushrooms,and achieving high-value utilization of edible mushroom by-products.The aim is to promote positive development …
Research Progress In Effects Of Electron Beam Irradiation On The Storage Andquality Of Grains,
2025
School of Grain , Oil and Management , Guangxi Technology and Business Vocational College , Nanning 530007 , China
Research Progress In Effects Of Electron Beam Irradiation On The Storage Andquality Of Grains, Xiuxia Chen, Bin Zhang
Journal of Food Science and Biotechnology
Conventional grain storage methods have a variety of drawbacks.Electron beam irradiation,as an emerging non-thermal processing technology,has attracted attention due to its high efficiency,environmental friendliness,and minimal impact on grain quality.The author elaborates on the principles of electron beam irradiation and its applications in the food industry,reviews its effects on harmful organisms,physicochemical properties,and sensory quality of grains during storage,and makes an outlook on its future application prospects,with the expectation of promoting the application and popularization of electron beam irradiation in food storage.
Directional Immobilization Of Mutant Β-Agarase Aga 50d Onto Magnetic Nanoparticles And Its Thermal Stability,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Directional Immobilization Of Mutant Β-Agarase Aga 50d Onto Magnetic Nanoparticles And Its Thermal Stability, Qiaoling Xie, Jie Long, Long Chen, Chao Qiu, Xing Zhou, Zhengyu Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to achieve site-directed immobilization of β-agarase at targeted residues,thereby enhancing its thermal stability and operational performance.[Method ] Computer-aided design was employed to selectively immobilize the mutant β-agarase Aga 50D via sulfhydryl groups introduced at specific cysteine residues,enabling binding to the carrier.[Result ] The immobilization conditions were optimized as follows:reaction duration of 12 h,an enzyme loading level (enzyme-to-carrier mass ratio ) of 1∶50,and a crosslinker-to-carrier mass ratio of 10∶50.The immobilization efficiency ranged from 40% to 75%,with most mutants retaining the relative enzyme activity > 80%.Compared with the wild-type free enzyme,the site-directed immobilized mutant β-agarase Aga 50D exhibited …
Ultrasonic-Microwave Assisted Extraction Of Flavonoids From Citri Reticulatae Pericarpium And High-Purity Purification Of Hesperidin,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Ultrasonic-Microwave Assisted Extraction Of Flavonoids From Citri Reticulatae Pericarpium And High-Purity Purification Of Hesperidin, Weiben Ge, Yiheng Li, Hongxin Wang
Journal of Food Science and Biotechnology
[Objective ] To explore the application prospects of Citri Reticulatae Pericarpium in the food industry and efficiently extract the active component hesperidin,while developing an effective purification process to enhance the purity and functional value of the active ingredients.[Method ] Ultrasonic-microwave assisted extraction (U-MAE ) was employed to extract the hesperidin from Citri Reticulatae Pericarpium,followed by a three-stage purification system guided by dissolution characteristics involving alcohol removal by concentration,gradient ethanol washing,and pH adjustment to purify hesperidin.Single factor experiments and response surface methodology were used to optimize the extraction parameters.The antioxidant activity and α-glucosidase inhibitory activity of the final product were also …
Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat,
2025
Life Sciences Department , Yuncheng University , Yuncheng 044099 , China;College of Food Science and Engineering , Shanxi Agricultural University , Taigu 030801 , China;Shanxi Technology Innovation Center of High Value-Added Echelon Utilization of Premium Agro-Products , Yuncheng 044099 , China
Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat, Qian Li, Xiaodong Li, Ziyi Yang, Yueyao Zhang, Rui Liu, Zhanglong Yu, Yuanlin Sun
Journal of Food Science and Biotechnology
[Objective ] In order to study the effects of germination on the mass fractions of phenolic acids in black wheat,a method was established for simultaneous determination of 11 phenolic acids (gallic acid,protocatechuic acid,4-hydroxybenzoic acid,chlorogenic acid,vanillic acid,caffeic acid,syringic acid,p-coumaric acid,ferulic acid,sinapic acid,and salicylic acid ) in germinated black wheat based on high performance liquid chromatography (HPLC ).[Method ] The mobile phase composed of methanol (containing acetic acid at a volume fraction of 1%) and water (containing acetic acid at a volume fraction of 1%) was used for gradient elution at a flow rate of 1 mL/min,a detection wavelength of 280 nm,and …
Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel, Aimiao Wang, Bo Wang, Yuchuan Wang, Jiguang Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims at solving the problems such as long drying time,single product shape,and poor 3D printing capacity (easy blockage and weak bracing ability ) in conventional processing of hawthorn snack products.[Method ] The low-sugar hawthorn jam and high-sugar banana jam were used as gel components.The 3D printing was carried out with the optimization of dual-material combination gel formula and the optimization of dual-nozzle 3D printing process.Furthermore,the drying characteristics of 3D printing were analyzed and compared with those of conventional dried hawthorn products.[Result ] The combination of hawthorn jam with the sugar mass fraction of 26% and banana …
Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods,
2025
Academy of Agriculture and Forestry Sciences , Qinghai University , Xining 810016 , China;Laboratory for Research and Utilization of Qinghai-Tibet Plateau Germplasm Resources , Xining 810016 , China;Key Laboratory of Qinghai-Tibetan Plateau Biotechnology (Qinghai University ), Ministry of Education , Xining 810016 , China;Key Laboratory of Agricultural Integrated Pest Management , Xining 810016 , China
Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods, Huanhuan Zhi, Yu Dong
Journal of Food Science and Biotechnology
[Objective ] This study aims to develop suitable packaging strategies for sweet cherry varieties with different respiratory characteristics.[Method ] The sweet cherry cultivars ‘Lapins’ and ‘Regina’ were put in closed systems with different O 2 volume fractions at 0,5,and 10 ℃.The changes in O 2 consumption rate,CO 2 consumption rate,and respiratory quotient were monitored.Additionally,the changes of gas components,quality,and physiological disorders were assessed for the two cultivars treated with polyethylene (PE) and modified atmosphere packaging (MAP ) after 4 weeks of storage at 0,5,and 10 ℃.[Result ] ‘Lapins’ had lower O 2 consumption rate and CO 2 consumption rate than ‘Regina’ …
Effects Of Ginger Protease On Beef Quality And Protein Degradation,
2025
School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China
Effects Of Ginger Protease On Beef Quality And Protein Degradation, Xinghua Chen, Shuhong Liu, Huaisheng Zheng, Zhaobin Huang, Ping Hu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of ginger protease on beef tenderness.[Method ] Ginger protease was extracted from mature ginger via ammonium sulfate precipitation.The fundamental properties of the ginger protease,including enzyme activity,protein mass fraction,optimal temperature and pH,and relative molecular mass,were evaluated.Furthermore,the effects of ginger protease on the physicochemical properties,sensory quality,microstructure,and protein degradation characteristics of beef were examined.[Result ] Ginger protease was mainly composed of two proteins with relative molecular masses of 15 293 and 34 191,with a total protein mass fraction of (574.60±0.25) mg/g,an enzyme activity of (31 787±307) U/g,an optimum temperature of 60 ℃,and an optimum …
Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol,
2025
Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Jiaxing Institute of Future Food , Jiaxing 314001 , China
Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol, Wenjie Zuo, Ping Zhang, Ruirui Xu, Leyi Zhang, Zhen Kang, Jianghua Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P.pastoris ) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method ] On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio,the gadusol fermentation pathway was constructed in P.pastoris via strategies such as promoter engineering,metabolic engineering,and fermentation optimization.[Result ] A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation.The gadusol yield in the recombinant strain P.pastoris HME reached 105 mg/L.By screening of gene expression promoters and optimization …
Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine,
2025
Institute of Microbiology , Guangdong Academy of Sciences , Guangzhou 510070 , China;Guangdong Detection Center of Microbiology , Guangzhou 510070 , China;State Key Laboratory of Applied Microbiology Southern China , Guangzhou 510070 , China;Guangdong Provincial Key Laboratory of Microbial Safety and Health , Guangzhou 510070 , China;National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food , Guangzhou 510070 , China
Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine, Zhenjie Liu, Peihao Huang, Shu Chen, Shun Rong, Feiting Peng, Ling Chen, Weipeng Guo
Journal of Food Science and Biotechnology
[Objective ] This study explored the effects of critical environmental factors [water activity (aw),pH,and temperature ] on the antimicrobial activity of ε-poly- L-lysine (ε-PL),aiming to provide a basis for the application of ε-PL.[Method ] With Staphylococcus aureus as an indicator strain,the micro-broth dilution and agar dilution methods as well as antimicrobial growth kinetics were utilized to determine the antimicrobial activity of ε-PL.The protein leakage assay was employed to evaluate the bacterial membrane disruption activity of ε-PL.Live/Dead fluorescence staining was performed to observe the bacterial membrane permeability.[Result ] The minimum inhibitory mass concentration (MIC ) of ε-PL against Staphylococcus aureus was …
Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation, Bo Zhai, Quanxian Shu, Xiaoyan Yang, Yin Mao, Yu Deng, Yunying Zhao
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the utilization of maize resources and address the shortage of feed resources.[Method ] The intestinal colonization capacity,tolerance,and antibacterial properties of three Lactobacillus strains,Lactobacillus paracasei ZB- 1 (ZB-1),L.paracasei DY- 2 (DY-2),and L.rhamnosus DY- 4 (DY-4) were evaluated.The most suitable strain for fermenting cornhusks was identified,and its fermentation process was optimized.[Result ] ZB-1 exhibited higher intestinal colonization ability and antibacterial properties than DY- 2 and DY- 4.The antibacterial mechanism of ZB- 1 was likely driven by the action of organic acids in an acidic environment and certain protein-based antimicrobial substances.The fermentation conditions for ZB- 1 …
Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang,
2025
College of Chemistry and Chemical Engineering , Xinjiang Normal University , Urumqi 830054 , China;Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Research Institute , Urumqi 830011 , China
Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang, Lei Sun, Laifang Qi, Qiuyan Liang, Jinxuan Han, Tianli Fan, Yuhua Ma
Journal of Food Science and Biotechnology
[Objective ] This study aims to understand the potential harmful fungi that may affect the quality of raisins during the drying process of grapes from Turpan,Xinjiang and to explore the relationships between varieties and the fungal community structure and diversity on the surfaces of grapes and raisins.[Method ] Four popular grape varieties (‘Thompson Seedles s’,‘Boert e’,‘Xiangfe i’,and ‘Seedless Blac k’) in a grapery in Turpan were selected as research objects.The fungal diversity on the surfaces of grapes and raisins was analyzed via high-throughput sequencing.[Result ] The dominant fungal phyla on the surfaces of grapes and raisins were Ascomycota and Basidiomycota.The …
Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model, Zeren Cheng, Xia Meng, Kejing Tang, Ling Shen, Qing Gu, Ping Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to compare the effects of hesperetin and eriocitrin on the gut microbiota in different enterotypes.[Method ] An in vitro gut fermentation model was established,which was combined with 16S rRNA high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS ) technique to evaluate the regulatory effects of the two flavonoids on the gut microbiota in different enterotypes.[Result ] After fermentation,both flavonoids significantly reduced the Firmicutes-to-Bacteroidota ratio (F/B ratio ) in terms of relative abundance at the phylum level in enterotype Bacteroides and enterotype Prevotella.At the genus level,pro-inflammatory bacteria including Paeniclostridium and Sutterella showed decreased relative abundance in treatment …
Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen,
2025
School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China
Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen, Xiong He, Rongxing Li, Shaotong Jiang, Jianfeng Lu, Lin Lin
Journal of Food Science and Biotechnology
[Objective ] The effects of different extraction methods on the yield,purity,structural characteristics,and physicochemical properties of tortoise shell collagen were investigated.[Method ] The ultrasound-assisted enzymatic method,acid method,and enzymatic method were used to extract collagen from tortoise shell powder.[Result ] The yield and purity of tortoise shell collagen extracted by ultrasound-assisted enzymatic method were 2.29% and 85.65%,respectively,significantly higher than those extracted by acid method (0.78% and 73.15%) and enzymatic method (1.77% and 75.63%).Ultraviolet absorption spectroscopy,infrared absorption spectroscopy,circular dichroism spectroscopy,and electrophoresis profiles showed that collagens extracted from tortoise shell by three methods were all type I collagen and retained intact triple helix structures.X-ray …
Comparison Of Pcr-Based Methods For The Detection Of Canned Tuna Species,
2025
Chapman University
Comparison Of Pcr-Based Methods For The Detection Of Canned Tuna Species, Chloe P. Castanon, Denise Hernandez, Akshay N. Khetrapal, Rosalee S. Hellberg
Food Science Faculty Articles and Research
Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna …
Natural Pigment-Mediated Photodynamic Antimicrobial Technology : Mechanisms And Food Applications,
2025
School of Food and Bioengineering , Zhengzhou University of Light Industry , Zhengzhou 450001 , China;Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation , Nanyang Institute of Technology , Nanyang 473004 , China
Natural Pigment-Mediated Photodynamic Antimicrobial Technology : Mechanisms And Food Applications, Lei Zhao, Wenli Jiang, Qingshan Shen, Jingxuan Ke, Yanli Ma, Lifang Zhang
Journal of Food Science and Biotechnology
Photodynamic antimicrobial technology (PDT ),as a non-thermal physical bactericidal method,has received increasing attention due to its high efficiency,low residue,and lack of resistance induction.Natural pigments,as food-grade photosensitizers exhibit excellent optical responsiveness and biocompatibility.Studies have shown that curcumin,chlorophylls,and porphyrin-based pigments exhibit favorable photodynamic antimicrobial efficacy in various food matrices including fruits,vegetables,meat,and drinking water.The author summarizes the photodynamic antimicrobial mechanisms of representative natural pigments,and analyzes the effects of light parameters,photosensitizer concentrations,and food matrices on photodynamic antimicrobial efficacy.Furthermore,the recent progress of PDT applications in fruit and vegetable surface disinfection,drinking water sterilization,and smart packaging is reviewed,with a focus on the role of nanocarriers and composite …
Research Progress In Aflatoxin Degradation Technologies For Edible Oils,
2025
School of Public Health , Guangxi Medical University , Nanning 530021 , China
Research Progress In Aflatoxin Degradation Technologies For Edible Oils, Huishuai Li, Zhenning Li, Yu Xie, Yunfeng Zou, Shaojun Li
Journal of Food Science and Biotechnology
Aflatoxin (AF),a notorious contaminant,has been a persistent challenge to the safety of edible oils.Prolonged exposure to low doses or acute consumption of high amounts of AF may induce serious health issues.Currently,no method can completely eliminate AF from edible oils.Therefore,it is crucial to explore strategies for reducing AF levels in edible oils.The measures to degrade AF from edible oils were reviewed,mainly including physical methods (such as high-temperature treatment,ray irradiation,and adsorption ),chemical methods (such as ammonia fumigation and ozone oxidation ),and biological methods (such as microbial degradation ).In addition,this paper analyzed and discussed the principles,advantages,shortcomings,and safety of various degradation methods.Furthermore,it made an …
Optimization Of Fermentation Conditions For The Removal Of Zearalenone From Corn Gluten Feed By Aspergillus Niger Fs10,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China ;Institute of Future Food Technology , JITRI , Wuxi 214200 , China
Optimization Of Fermentation Conditions For The Removal Of Zearalenone From Corn Gluten Feed By Aspergillus Niger Fs10, Mengying Wu, Yang Yang, Jinyao Wang, Yongli Ye, Li’Na Sheng, Jian Ji, Xiulan Sun
Journal of Food Science and Biotechnology
[Objective ] This study aims to determine the optimal solid-state fermentation conditions for the removal of zearalenone (ZEN ) from corn gluten feed by Aspergillus niger (A.niger ) FS10.[Method ] With the removal rate of ZEN as the indicator,single-factor experiments and response surface method were employed to optimize the key fermentation parameters (inoculation volume-to-mass ratio,fermentation temperature,fermentation duration,and solid-to-liquid ratio ).Furthermore,changes in mass fractions of key nutritional components in corn gluten feed before and after solid-state fermentation under the optimal conditions were analyzed.[Result ] The optimal fermentation conditions were established as follows:an inoculation volume-to-mass ratio of 120 μL∶1 g,a fermentation temperature …
