Investigation Of Congestive Heart Failure In Beef Cattle In A Feedyard At A Moderate Altitude In Western Nebraska,
2019
University of Nebraska - Lincoln
Investigation Of Congestive Heart Failure In Beef Cattle In A Feedyard At A Moderate Altitude In Western Nebraska, Rodney A. Moxley, David R. Smith, Dale M. Grotelueschen, Tom Edwards, David Steffen
School of Veterinary and Biomedical Sciences: Faculty Publications
Right-sided congestive heart failure (brisket disease) commonly occurs in cattle raised at elevations >2,500– 3,500 m. We investigated clinical cases resembling brisket disease at a western Nebraska feedyard at a moderate altitude (1,369 m). Over a 15-mo period (2009–2010), we examined 17 cases (16 steers and 1 heifer), all purebred Angus. All animals had clinical right-sided heart failure: brisket and ventral abdominal edema, and severe chronic passive congestion of the liver. Gross examination confirmed right ventricular hypertrophy (left ventricle plus septum: right ventricle weight ratio mean: 1.33 vs. 2.8–4.0 reference interval). Microscopically, all 17 cases had interstitial fibrosis (mean score: …
Discovery And Characterization Of Novel Substrate Selective Inhibitors Of Human Mrp1 (Abcc1),
2019
South Dakota State University
Discovery And Characterization Of Novel Substrate Selective Inhibitors Of Human Mrp1 (Abcc1), Angelina Sampson
Electronic Theses and Dissertations
Multidrug resistance protein 1 (MRP1/ABCC1) actively transports a variety of drugs, toxic molecules and important physiological substrates across the plasma membrane. It can confer broad-spectrum drug resistance and can decrease the bioavailability of many important drugs such as anti-cancer agents, antibiotics, antivirals, antidepressants and antiinflammatory drugs. Calcein-AM, a fluorescent reporter commonly used for studying compound interactions with MRP1 was recently used in the development of a high content imaging-based assay by our group. This assay was robust and had better sensitivity than fluorescent plate readouts. The assay identified 12 MRP1 inhibitors after screening an anticancer library of 386 compounds. Due …
Stabilization Of Dna I-Motif Structures By 7-Aminoactinomycin D, An Anti-Tumor Drug,
2019
University of Mississippi
Stabilization Of Dna I-Motif Structures By 7-Aminoactinomycin D, An Anti-Tumor Drug, Justin Lane Parmely
Electronic Theses and Dissertations
Alternative DNA structures are likely to form from Watson-Crick B-form DNA when antitumor drug known to bind DNA loops -- can affect the iM structure. Our results demonstrate as an i-motif (iM). While both structures are known to exist in vivo they are energetically uphill can utilize intercalating cytosine-cytosine base pairing to form a four-stranded structure known controlled by alternative DNA structures like G4s and iMs. especially during processes that involve superhelical duress. A guanosine rich strand can form a facilitate their stabilization. In this report we present data on how 7-aminoactinomycin D -- an formation. Earlier it was believed …
Estimating The Age Of A Bloodstain Using Droplet Digital Pcr,
2019
West Virginia University
Estimating The Age Of A Bloodstain Using Droplet Digital Pcr, Kalee Rae Crampton
Graduate Theses, Dissertations, and Problem Reports (ETD)
Biological evidence is extremely valuable in the investigation of a crime due to the presence of DNA. DNA evidence is considered the gold standard in court cases due to its ability to link a suspect to a piece of evidence. In addition to DNA evidence, biological stains have the potential to provide a temporal link between an individual and a crime scene. Previous studies have shown that relative rates of RNA degradation can be used in order to estimate the age of bloodstains. Here, we examined the ability of droplet digital PCR to be used in place of quantitative PCR …
Insights Into Nucleic Acid-Platinum(Ii) Compound Interactions And Structural Impacts,
2019
Wayne State University
Insights Into Nucleic Acid-Platinum(Ii) Compound Interactions And Structural Impacts, Supuni Duneeshya Kamal Thalalla Gamage
Wayne State University Dissertations
With the discovery of cisplatin in the 1960s, it has been widely studied as a precursor for anticancer drug development. Despite its effectiveness against certain cancers, clinical usage of cisplatin is restricted by a number of side effects and resistance. In the past decade, scientists have been exploring biologically important ligands such as sugar derivatives in the hope of overcoming such challenges. Attachment of a sugar moiety could facilitate lower accumulation of platinum drugs in the body as well as enhance cellular uptake. In this study, a carbohydrate-linked cisplatin analog, cis-dichlorido[(2-β-D-glucopyranosidyl)propane-1,3-diammine]platinum (5) has been studied. The aim was to evaluate …
Giardia Lamblia Growth In Viscoelastic Fluids,
2019
Claremont McKenna College
Giardia Lamblia Growth In Viscoelastic Fluids, Kelly Watanabe
CMC Senior Theses
Giardia lamblia is a single-celled protozoan parasite that when ingested, causes diarrheal disease and infects 33% of people in developing countries. Previous studies observe Giardia in water-like fluids, but Giardia's infectious environment consists of viscoelastic mucus in the small intestine. Therefore, Giardia was cultured in viscoelastic fluids, and its population growth was observed in vitro. To create shear-thinning viscoelastic fluids, 0.2% and 0.4% long-chain polyacrylamide (LCPAM) was added to cell culture media. Giardia was cultured in control media, 0.2% LCPAM, and 0.4% LCPAM, and population growth was quantitatively determined over time. Increasing LCPAM concentration resulted in a solution with …
The Fallacy Of Defensive Protection For Traditional Knowledge,
2019
Emory University School of Law
The Fallacy Of Defensive Protection For Traditional Knowledge, Margo A. Bagley
Faculty Articles
Proponents of databases as defensive protection posit that having sources of traditional knowledge easily accessible to, and searchable by, examiners during the prosecution process should minimize the grant of patents covering traditional knowledge, and avoid the problems such erroneously granted patents may produce. Some countries, such as India, which support an international sui generis positive protection instrument, also support the use of traditional knowledge databases, as the two approaches are not mutually exclusive. India's CSIR, which created and maintains the TKDL, asserts that the database has thwarted the grant of scores of patents in IP offices across the globe, although …
Computational Design And Production Of Levansucrase With Increased Thermostability,
2019
Faculty of Science
Computational Design And Production Of Levansucrase With Increased Thermostability, Methus Klaewkla
Chulalongkorn University Theses and Dissertations (Chula ETD)
Levansucrase is a carbohydrate-modifying enzyme that catalyzes hydrolysis of sucrose and transfructosylation of a fructosyl unit of sucrose to produce levan and levan-type fructooligosaccharides (LFOs). Due to their various beneficial properties such as low viscosity and non-toxicity. Levan and LFOs have potential applications in many industries such as prebiotics in food industries and anti-tumor agents in pharmaceutical industries. Previous study found that the Y246S mutant of Bacillus licheniformis RN-01 levansucrase (Oligosaccharide Producing Levansucrase, OPL) could effectively produce LFOs, but its thermostability is limited at high temperature. In this study, molecular dynamics (MD) and computational protein design were used to design …
Effects Of Aquatic Acidification On Calcium Uptake In White River Shrimp Litopenaeus Setiferus Gills,
2019
University of North Florida
Effects Of Aquatic Acidification On Calcium Uptake In White River Shrimp Litopenaeus Setiferus Gills, Maria-Flora Jacobs
UNF Graduate Theses and Dissertations
Previous research regarding aquatic acidification has examined the protonation of the carbonate and does not consider calcium to be a limiting factor. This is the first study to suggest that pH may affect the uptake of calcium in crustacean gills. This project describes ion transport mechanisms present in the cell membranes of white river shrimp Litopenaeus setiferus gill epithelium, and the effects of pH on the uptake of calcium by these means. Partially purified membrane vesicles (PPMV) of shrimp gills were prepared through a homogenization process that has been used previously to define ion transport in crab and lobster gill …
The Relationship Between Seasonal Breeding And Deiodinase Expression In The Green Anole Lizard,
2019
Minnesota State University, Mankato
The Relationship Between Seasonal Breeding And Deiodinase Expression In The Green Anole Lizard, Hyejoo Kang
All Graduate Theses, Dissertations, and Other Capstone Projects
Reproductive physiology and behavior is mainly regulated by the hypothalamus-pituitary-gonad (HPG) axis. Interestingly, abnormal levels of thyroid hormone (TH) results in the delayed onset of puberty and affects gonadal function of adults by altering HPG axis activity. Seasonally breeding animals undergo drastic hormonal and behavioral changes between breeding and non-breeding seasons. Green anole lizards (Anolis carolinensis), similar to other seasonally breeding animals, have increased sex steroid hormones, larger gonads, upregulated gonadal steroidogenic acute regulatory protein (StAR) mRNA and increased reproductive behavior during the breeding compared to non-breeding seasons. Relatively less is known regarding the regulation of gonadal TH in seasonal …
Mutations Of Fus Cause Aggregation Of Rna Binding Proteins, Disruptions In Protein Synthesis, And Dysregulation Of Nonsense Mediated Decay,
2019
University of Kentucky
Mutations Of Fus Cause Aggregation Of Rna Binding Proteins, Disruptions In Protein Synthesis, And Dysregulation Of Nonsense Mediated Decay, Marisa Elizabeth Kamelgarn
Theses and Dissertations--Toxicology and Cancer Biology
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by motor neuron death and subsequent muscle atrophy. Approximately 15% of ALS cases are inheritable, and mutations in the Fused in Sarcoma (FUS) gene contribute to approximately 5% of these cases, as well as about 2% of sporadic cases. FUS performs a diverse set of cellular functions, including being a major regulator of RNA metabolism. FUS undergoes liquid- liquid phase transition in vitro, allowing for its participation in stress granules and RNA transport granules. Phase transition also contributes to the formation of cytoplasmic inclusions found in the …
Molecular Level Comparison Of Water Extractives Of Maple And Oak With Negative And Positive Ion Esi Ft-Icr Mass Spectrometry,
2019
Southern Regional Research Center, USDA Agricultural Research Service
Molecular Level Comparison Of Water Extractives Of Maple And Oak With Negative And Positive Ion Esi Ft-Icr Mass Spectrometry, Zhongqi He, Rachel L. Sleighter, Patrick G. Hatcher, Shasha Liu, Fengchang Wu, Haixuan Zou, O. Modesto Olanya
Chemistry & Biochemistry Faculty Publications
Soluble extractives in wood function to protect living trees from destructive agents and also contribute to wood color and fragrance. Some extractive components have biological activities with medical applications. They also play important roles in wood processing and related applications. To increase the knowledge of wood chemistry, maple and oak were extracted by water. Ultraviolet/visible (UV/vis) spectroscopy indicated the presence of a phenolic compound, resorcinol, in maple extractives having higher molecular mass and more aromatic components than oak extractives. Negative and positive electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS) identified thousands of formulas in the two …
Island Invasion: The Silent Crisis In Hawaii,
2019
Claremont Colleges
Island Invasion: The Silent Crisis In Hawaii, Sophia Janssen
Pomona Senior Theses
Keeping out invasive species may, upon first review, seem like a trivial environmental cry from ecologists and deep environmentalists; a belated wish to return to an undeveloped world where nature was pristine. However invasive species create problems that impact all of us and can have far more severe consequences than changing a stunning landscape. These problems are heightened in islands like Hawaii, where the fragile ecosystems have developed over centuries of evolution and adaptation. The introduction of a disease-carrying mosquito can put the people of Hawaii at risk to many vector-born illnesses and create an epidemic, taking human life. The …
Condensation And Polymerization Explain The Humification Of Lignin Into Aliphatic And Aromatic Structures In Soil,
2019
Old Dominion University
Condensation And Polymerization Explain The Humification Of Lignin Into Aliphatic And Aromatic Structures In Soil, Patrick G. Hatcher, Hongmei Chen, Seyyedhadi Khatami, Derek C. Waggoner
Chemistry & Biochemistry Faculty Publications
Soil organic matter (SOM) constitutes a global reservoir of carbon that is more than twice that of either atmospheric carbon or aquatic carbon; however, the manner in which it forms from degraded plant biomass is poorly understood. Some have recently questioned whether plant biomass is involved directly in SOM formation and suggest that it is microbial carbon that constitutes the main source of stable SOM. Such a view implies that above and below ground plant biomass is rapidly decomposed and mineralized. This view contrasts significantly with traditional ones that involve the transformation of plant biomass to recalcitrant humic materials fueled …
Treatment Of Plastic Wastes Using Plasma Gasification Technology,
2019
University of Nebraska-Lincoln
Treatment Of Plastic Wastes Using Plasma Gasification Technology, Zachary Homolka
Honors Program: Senior Projects (Public)
Plasma gasification (PG) complements traditional recycling when applied to contaminated or mixed plastics. Without PG these plastics cost recyclers more to process than they are worth on the market, and sometimes they are landfilled or incinerated instead of being recycled. Plasma gasification can take plastic not suitable for traditional recycling and break it down into high-quality syngas for use in electricity generation, chemical manufacturing, or hydrogen production. The technology can be implemented without changing the behavior of consumers, which is a major advantage over attempting to decrease contamination or reduce use of low-value plastic. Due to high capital requirements and …
Amino Acids Profiling For The Diagnosis Of Metabolic Disorders,
2019
Cleveland State University
Amino Acids Profiling For The Diagnosis Of Metabolic Disorders, Yana Sandlers
Chemistry Faculty Publications
Inborn errors of metabolism (IEM) represent a group of inherited diseases in which genetic defect leads to the block on a metabolic pathway, resulting in a single enzyme dysfunction. As a downstream consequence of the residual or full loss of the enzymatic activity, there is an accumulation of toxic metabolites in the proximity of the metabolic block and/or a deficiency of an essential metabolic product which leads to the clinical presentation of the disease. While individually IEMs are rare, a collectively estimated incidence of metabolic inherited disorders is 1:800. The genetic basis of IEMs can involve abnormalities such as point …
Unintended Consequences Of Dna Analysis Delays In North Carolina,
2019
Walden University
Unintended Consequences Of Dna Analysis Delays In North Carolina, Pamela Cook Woodard
Walden Dissertations and Doctoral Studies
The processing of DNA recovered from felony crime scenes often causes delays in trials of up to 3 years, calling into question defendants' rights to a speedy trial. Using Lewin's force field analysis as the theoretical framework, the purpose of this quantitative, comparative study was to compare the processing and reporting of results related to DNA testing in 4 states. Survey data were collected from state bar members (n=137), members of a professional law organization (n=149), and members of a state DNA laboratory (n=20). The purpose of this quantitative, comparative study was to determine whether these variables (interagency communications, staff …
Toward An Enzyme-Coupled, Bioorthogonal Platform For Methyltransferases: Probing The Specificity Of Methionine Adenosyltransferases,
2019
University of Kentucky
Toward An Enzyme-Coupled, Bioorthogonal Platform For Methyltransferases: Probing The Specificity Of Methionine Adenosyltransferases, Tyler D. Huber
Theses and Dissertations--Pharmacy
Methyl group transfer from S-adenosyl-l-methionine (AdoMet) to various substrates including DNA, proteins, and natural products (NPs), is accomplished by methyltransferases (MTs). Analogs of AdoMet, bearing an alternative S-alkyl group can be exploited, in the context of an array of wild-type MT-catalyzed reactions, to differentially alkylate DNA, proteins, and NPs. This technology provides a means to elucidate MT targets by the MT-mediated installation of chemoselective handles from AdoMet analogs to biologically relevant molecules and affords researchers a fresh route to diversify NP scaffolds by permitting the differential alkylation of chemical sites vulnerable to NP MTs that are unreactive to …
The Involvement Of Progesterone In Glial Cell Viability,
2019
Columbus State University
The Involvement Of Progesterone In Glial Cell Viability, Tracee L. Guthrie
Theses and Dissertations
We studied the effects of ethynodiol diacetate on the viability of hydrogen peroxide stressed glial cells. We wanted to determine the extent in which varying concentration of ethynodiol diacetate affect cell viability of cultured glial cells from hydrogen peroxide toxicity. Based on previous studies, we predicted that the viability of hydrogen peroxide-stressed cells will increase with the presence of the progesterone, ethynodiol diacetate, and that cell viability will increase with increasing concentration of ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% …
The Effects Of Ethynodiol Diacetate On Epinephrine-Treated Glial-Derived Cells,
2019
Columbus State University
The Effects Of Ethynodiol Diacetate On Epinephrine-Treated Glial-Derived Cells, Jesse D. Hunt
Theses and Dissertations
Neuroprotective agents, including specific hormones, may play a role in preventing cellular damage following an ischemic event or brain injury. Astrocytes are specialized glial cells within the central nervous system that play roles in synapse development, blood flow regulation, extracellular homeostasis, synapse function, energy and metabolism (Sofroniew and Vinters, 2009). Research using astrocytes in cell viability studies can be beneficial in deepening our understanding of specific neuroprotective effects of various steroid hormones including progesterone. Progesterone has been shown to have neuroprotective capabilities in the central nervous system (Scarpin et ah, 2009). Although progesterone shows great therapeutic potential, effects of the …
