Synthesis, Structure Elucidation And Selected Biological Activities Of Ag(I) And Au(I) Complexes Constructed From Thiosemicarbazone And Phosphine Ligands,
2020
Universiti Malaya
Synthesis, Structure Elucidation And Selected Biological Activities Of Ag(I) And Au(I) Complexes Constructed From Thiosemicarbazone And Phosphine Ligands, Syahrina Nur ‘Ain Abdul Halim
Student Works (2020-2029)
New Ag(I) and Au(I) mixed ligand complexes were synthesised using thiosemicarbazone and phosphine in search of a new biologically active compounds. CHN elemental analysis, powder X-ray diffraction (PXRD), single crystal X-ray diffraction as well as several spectroscopic techniques such as FT-IR, Energy-dispersive X-ray (EDX), 1H, 13C, and 31P{1H} NMR were performed to elucidate the structure of these complexes. Elemental analysis suggested that the stoichiometry of the complexes formed by the reaction of Ag nitrate with thiosemicarbazone in the presence of phosphine was indeed 1:2:1 molar ratio, while for the Au to be in 1:1:1 molar ratio. The Ag and Au …
Pressure Driven Desalination Utilizing Nanomaterials,
2020
calpoly san luis obispo
Pressure Driven Desalination Utilizing Nanomaterials, Fangyou Xie
Master's Theses
Nanomaterials such as graphene oxide and carbon nanotubes, have demonstrated excellent properties for membrane desalination, including decrease of maintenance, increase of flux rate, simple solution casting, and impressive chemical inertness. Here, two projects are studied to investigate nanocarbon based membrane desalination. The first project is to prepare hybrid membranes with amyloid fibrils intercalated with graphene oxide sheets. The addition of protein amyloid fibrils expands the interlayer spacing between graphene oxide nanosheets and introduces additional functional groups in the diffusion pathways, resulting in increase of flux rate and rejection rate for the organic dyes. Amyloid fibrils also provide structural assistance to …
Microglia Induced Neuroinflammation Through The Nlrp3 Inflammasome Following Blast Traumatic Brain Injury,
2020
New Jersey Institute of Technology
Microglia Induced Neuroinflammation Through The Nlrp3 Inflammasome Following Blast Traumatic Brain Injury, Daniel Younger
Dissertations
The incidence of traumatic brain injury (TBI) among military personnel have been steadily increasing with modern conflicts. A recent RAND report estimated 320,000 service members, totaling 20% of deployed forces, suffer from TBI. However, of this population roughly 60% have not seen a medical professional specifically for TBI. Unlike the civilian population, the primary cause of TBI for active-duty military personnel is blast exposure. Blasts now account for over 70% of all US military casualties in operation Iraqi Freedom (OIF) and Operation enduring freedom (OEF) and are the major cause of TBI. Among many pathological mechanisms associated with blast TBI, …
Capsaicin Is A Negative Allosteric Modulator Of The 5-Ht3 Receptor,
2020
UAE University
Capsaicin Is A Negative Allosteric Modulator Of The 5-Ht3 Receptor, Eslam El Nebrisi, Tatiana Prytkova, Dietrich Ernst Lorke, Luke Howarth, Asma Hassan Alzaabi, Keun-Hang Susan Yang, Frank Christopher Howarth, Murat Oz
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
In this study, effects of capsaicin, an active ingredient of the capsicum plant, were investigated on human 5-hydroxytryptamine type 3 (5-HT3) receptors. Capsaicin reversibly inhibited serotonin (5-HT)-induced currents recorded by two-electrode voltage clamp method in Xenopus oocytes. The inhibition was time- and concentration-dependent with an IC50 = 62 μM. The effect of capsaicin was not altered in the presence of capsazepine, and by intracellular BAPTA injections or trans-membrane potential changes. In radio-ligand binding studies, capsaicin did not change the specific binding of the 5-HT3 antagonist [3H]GR65630, indicating that it is a noncompetitive inhibitor of …
Investigating Photosynthetic Stability: Relation Between Thylakoid Lipid Content And The Stability Of The Cytochrome B6f Complex,
2020
Purdue University
Investigating Photosynthetic Stability: Relation Between Thylakoid Lipid Content And The Stability Of The Cytochrome B6f Complex, Marina Mehling
The Journal of Purdue Undergraduate Research
The cytochrome b6f complex is an enzyme found in plants, cyanobacteria, and green algae that catalyzes the transport of electrons in the rate-limiting step of oxygenic photosynthesis. This dimeric complex has an extensive lipid architecture that is primarily composed of five distinct lipid classes: monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), phosphatidyl glycerol (PG), monoglucosyl diacylglycerol (GlcDG), and sulfoquinovosyl diacylglycerol (SQDG). While these lipid classes have been identified, their precise role in the function of the cytochrome complex are only beginning to be understood. Mechanisms describing the relation between thylakoid lipid content on the stability of the b6 …
Aerosol Mass And Optical Properties, Smoke Influence On O3, And High No3 Production Rates In A Western U.S. City Impacted By Wildfires,
2020
University of Montana
Aerosol Mass And Optical Properties, Smoke Influence On O3, And High No3 Production Rates In A Western U.S. City Impacted By Wildfires, Vanessa Selimovic, Robert J. Yokelson, Gavin R. Mcmeeking, Sarah Coefield
Chemistry and Biochemistry Faculty Publications
Evaluating our understanding of smoke from wild and prescribed fires can benefit from downwind measurements that include inert tracers to test production and transport and reactive species to test chemical mechanisms. We characterized smoke from fires in coniferous forest fuels for >1,000 hr over two summers (2017 and 2018) at our Missoula, Montana, surface station and found a narrow range for key properties. ΔPM2.5/ΔCO was 0.1070 ± 0.0278 (g/g) or about half the age-independent ratios obtained at free troposphere elevations (0.2348 ± 0.0326). The average absorption Ångström exponent across both years was 1.84 ± 0.18, or about half the values …
Modulation Of Escherichia Coli Translation By The Specific Inactivation Of TrnaGly Under Oxidative Stress,
2020
Universidad de Chile
Modulation Of Escherichia Coli Translation By The Specific Inactivation Of TrnaGly Under Oxidative Stress, Lorenzo Eugenio Leiva, Andrea Pincheira, Sara Elgamal, Sandra D. Kienast, Verónica Bravo, Johannes Leufken, Daniela Gutiérrez, Sebastian A. Leidel, Michael Ibba, Assaf Katz
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Bacterial oxidative stress responses are generally controlled by transcription factors that modulate the synthesis of RNAs with the aid of some sRNAs that control the stability, and in some cases the translation, of specific mRNAs. Here, we report that oxidative stress additionally leads to inactivation of tRNAGly in Escherichia coli, inducing a series of physiological changes. The observed inactivation of tRNAGly correlated with altered efficiency of translation of Gly codons, suggesting a possible mechanism of translational control of gene expression under oxidative stress. Changes in translation also depended on the availability of glycine, revealing a mechanism whereby bacteria …
Testing The Combined Module Swapping And Repair By Modification Strategies: A Step Toward Universal Toolbox,
2020
University of Texas at Tyler
Testing The Combined Module Swapping And Repair By Modification Strategies: A Step Toward Universal Toolbox, Catherine A. Martini
Biology Theses
The crux of synthetic biology is the engineering of biological components to modulate the activity of specific DNA-based promoter(s) to drive gene expression; thus, providing a means to modulate pathways between signal detection and cellular response. However, the longstanding frustration of synthetic biologists has been the inability to transfer those engineered components between cellular systems — this lack of “modular universality” or “universal toolbox” impedes research by forcing a metaphorical reinvention of the wheel in new systems. The modular swapping strategy expanded the available “toolbox” with customizable hybrid repressors formed from “swapping” the DNA-recognition modules (DRMs) and environmental-sensing modules (ESMs) …
Analysis Of Olfactory Mrna Cap Modifications Using Rnaseh And Lc-Ms,
2020
CUNY Hunter College
Analysis Of Olfactory Mrna Cap Modifications Using Rnaseh And Lc-Ms, Ilinca M. Giosan
Theses and Dissertations
In the olfactory system, olfactory sensory neurons (OSNs) express odorant receptors (ORs) in a singular fashion through unknown mechanisms. Published data supports the notion that singularity is achieved, in part, through a modification the mRNA strand, perhaps in its 5’cap region. This theoretical study aims to test this 5’cap hypothesis.
Building An Ins-1 Cdna Library For A Genome-Wide Crispr-Cas9 Screen,
2020
Brigham Young University
Building An Ins-1 Cdna Library For A Genome-Wide Crispr-Cas9 Screen, Idongesit Ekpo
Undergraduate Honors Theses
By the year 2040, an estimated 642 million people are expected to have diabetes globally. Diabetes results from an elevation of metabolic stressors, such as glucotoxicity, lipotoxicity, oxidative stress and apoptosis. In type 2 diabetes, these stressful conditions contribute to the malfunction and loss of functional insulin-producing β-cells. Current treatment methods for diabetes include insulin therapy, islet transplant and anti-diabetes medication. These treatments are not curative and ignore other factors that contribute to the pathogenesis of diabetes beyond insulin resistance and islet β-cell failure. Previous research on β-cells has focused on ways to replace functional β-cell mass, trigger β-cell proliferation, …
Stormwater Capture Via Green Infrastructure In The Plaster Creek Watershed,
2020
Calvin University
Stormwater Capture Via Green Infrastructure In The Plaster Creek Watershed, Brett Barents, Kiara Bromley, Haley Weesies, Deanna Geelhoed, David P. Warners
Student Papers and Reports
No abstract provided.
Plaster Creek Stewards Green Team Initiative,
2020
Calvin University
Plaster Creek Stewards Green Team Initiative, Michael Akpabey, David E. Martinez Vasquez, David P. Warners
Student Papers and Reports
No abstract provided.
Exploring In Vivo And In Vitro Regulation Of The Shigella Type Three Secterion System Atpase Spa47,
2020
Utah State University
Exploring In Vivo And In Vitro Regulation Of The Shigella Type Three Secterion System Atpase Spa47, Heather B. Case
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Diarrheal disease is caused by a diverse group of enteric pathogens and is one of the leading causes of preventable deaths worldwide. Some of the most common causes of diarrheal disease are bacterial pathogens such as Salmonella, E. coli, Cholera, and Shigella. Many bacterial enteric pathogens use a type three secretion system (T3SS) to cause infection. The T3SS uses a needle and syringe like apparatus that allows the bacteria to inject proteins into the host cell to cause infection. Striking similarities in T3SSs from all pathogens that utilize them makes the T3SS an attractive target for …
Comparative Transcriptomics Of Rice Genotypes With Contrasting Responses To Nitrogen Stress Reveals Genes Influencing Nitrogen Uptake Through The Regulation Of Root Architecture,
2020
Louisiana State Univ, Agr Ctr, Sch Plant Environm & Soil Sci, Baton Rouge, LA
Comparative Transcriptomics Of Rice Genotypes With Contrasting Responses To Nitrogen Stress Reveals Genes Influencing Nitrogen Uptake Through The Regulation Of Root Architecture, Prasanta K. Subudhi, Richard S. Garcia, Sapphire Coronejo, Ronald Tapia
Faculty Publications
The indiscriminate use of nitrogenous fertilizers continues unabated for commercial crop production, resulting in air and water pollution. The development of rice varieties with enhanced nitrogen use efficiency (NUE) will require a thorough understanding of the molecular basis of a plant's response to low nitrogen (N) availability. The global expression profiles of root tissues collected from low and high N treatments at different time points in two rice genotypes, Pokkali and Bengal, with contrasting responses to N stress and contrasting root architectures were examined. Overall, the number of differentially expressed genes (DEGs) in Pokkali (indica) was higher than in Bengal …
Multifaceted Mechanism Of Vps1 Mediated Endosome-To-Golgi Fusion In Vitro,
2020
Missouri State University
Multifaceted Mechanism Of Vps1 Mediated Endosome-To-Golgi Fusion In Vitro, Ehsan Suez
Graduate Theses/Dissertations
To maintain cell homeostasis, protein recycling through intracellular membrane fusion is an important cellular process. Both Vps1 and Ypt6 have been implicated in protein recycling from the endosome to the trans-Golgi Network (TGN). SNARE proteins are thought to be the key regulator in this membrane fusion mediated protein recycling mechanism. I studied membrane fusion events by incorporating purified proteins into liposomes. A series of data suggest that high concentration of SNARE proteins inhibits fusion unlike the opposite popular notion. Also, the data suggests that Vps1 acts on membrane fusion dynamics in a manner that lower concentrations of Vps1 enhance …
Application Of Carbon Nanoparticles As Dna Detection Probe And Fluorescent Ink,
2020
Missouri State University
Application Of Carbon Nanoparticles As Dna Detection Probe And Fluorescent Ink, Luckio Frank Owuocha
Graduate Theses/Dissertations
There is a significant interest in developing a sensitive, selective, efficient, and inexpensive method for rapid molecular diagnostic tests. This research aims to develop an inexpensive nucleic acid detection method by using DNA-conjugated carbon nanoparticles that exhibit fluorescence in the visible region. Carbon nanoparticles of this class can be detected without specialized equipment and have great promise toward the development of analytical methods that can be used in resource-limited environments with a lack of access to proper diagnostic and healthcare. We employed EDC-NHS (two-step) and EDC (one-step) coupling techniques to prepare DNA-conjugated carbon nanoparticles. The dot blotting method was adapted …
Characterization Of Human Pyrroline-5-Carboxylate Reductase Enzymes Responsible For L-Proline Biosynthesis,
2020
University of Nebraska-Lincoln
Characterization Of Human Pyrroline-5-Carboxylate Reductase Enzymes Responsible For L-Proline Biosynthesis, Sagar Patel
Department of Biochemistry: Dissertations, Theses, and Student Research
Pyrroline-5-carboxylate reductases (EC 1.5.1.2) are important housekeeping enzymes of L-proline biosynthesis, which generate L-proline and influence redox cycling of NAD(P)H/NAD(P)+ to support cellular growth in all domains of life. Structural evidence from X-ray crystal structures of HsPYCR1 (PDB codes 5UAT, 5UAU, and 5UAV) shows both NADPH bound in the N-terminal Rao-Rossmann fold motif and an important hydrogen bond or proton donor role for Thr238 with L-P5C. The Thr238Ala mutation results in 10-fold loss in catalytic efficiency with varied L-P5C relative to the wild-type enzyme, thus indicating Thr238’s potential hydrogen bond and proton donation to L-P5C is critical for catalysis. …
The Relationship Between Cell-Free Dna And Resistance Training,
2020
East Tennessee State University
The Relationship Between Cell-Free Dna And Resistance Training, Henry Lang
Electronic Theses and Dissertations
The primary purposes of this dissertation were to explore relationship between cell free DNA (cf-DNA), creatine kinase (CK), C-reactive protein (CRP), vertical jump testing delayed onset muscle soreness (DOMS) in response to a high-volume resistance training protocol, and to assess the sensitivity of cf-DNA to different resistance training volume loads. The secondary purpose was to examine the relationship between cf-DNA and relative strength. Study 1 was an exploratory attempt to discover relationships between cf-DNA, CK, CRP, delayed onset muscle soreness, and performance variables. Seventeen resistance trained males were recruited, 9 were randomly assigned to receive BCAAs while 8 received a …
Artificial Intron Technology To Generate Conditional Knock-Out Mice,
2020
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Artificial Intron Technology To Generate Conditional Knock-Out Mice, Amber N. Thomas-Gordon
Dissertations and Theses (Open Access)
Genetic engineering has been re-shaped by the invention of new tools in modern biotechnology in a way that offers precision and efficiency in modifying the genome at a single nucleotide level and/or allowing precise control of gene expression. Such gene manipulation brings about significant findings and revelations in comprehending more about embryonic development, cellular and physiological functions, and disease pathology. Current methods used to produce conditional knockouts have limitations on conditional allele placement and modification varies among genes in different organisms. Thus, a system for generating conditional alleles with fidelity remains a challenge. My goal was to examine an approach …
Investigating Chitosan Modified With Triethylammonium Butanamide And Triethylphosphonium Butanamide As Non-Viral Gene Delivery Vectors By Examining Cytotoxicity And Transfection Efficiency,
2020
Missouri State University
Investigating Chitosan Modified With Triethylammonium Butanamide And Triethylphosphonium Butanamide As Non-Viral Gene Delivery Vectors By Examining Cytotoxicity And Transfection Efficiency, Deborah C. Ehie
Graduate Theses/Dissertations
Gene therapy is a very challenging field, especially with new emerging genetic disorders. Chitosan (CS), due to chitosan’s flexibility, biocompatibility, and biodegradability, has been of interest in the world of gene therapy especially as researchers are gravitating towards non-viral vectors due to the problems caused by viral vectors. Nevertheless, there are still issues regarding solubility, cellular uptake of cargos being transported in vitro or in vivo, increased cytotoxicity levels, as well as many other things that prevent chitosan from being an efficient gene delivery agent. Here I present five derivatives of chitosan, which were all modified with either triethylphosphonium …
