Agriculture Commons^™

Genomic And Transcriptomic Studies In Mycobacterium Avium Subspecies Paratuberculosis, J. P. Bannantine, Adel M. Talaat

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Microarray technology is an important tool in functional genomic research. It has enabled a deeper analysis of genomic diversity among bacteria belonging to the Mycobacterium avium complex (MAC). In addition, the expression of thousands of genes can be studied simultaneously in a single experiment. With the complete genome sequence of a bovine isolate of M. avium subspecies paratuberculosis, and the independent construction of DNA microarrays in our laboratories, transcriptomic studies for this veterinary pathogen are now possible. Furthermore, the bovine genome sequence project is completed and bovine arrays have been developed to examine host responses to infection with M. avium …

Influences Of Wind And Sandblasting On The Endangered Blowout Penstemon, James Stubbendieck, Cheryl D. Dunn, Heidi L. Hillhouse, L. M. Landholt

Department of Agronomy and Horticulture: Faculty Publications

Blowout penstemon Penstemon haydenii is a federally endangered species growing only in areas with active wind erosion in sand dunes of the central United States. This early seral species declines as the blowout habitat stabilizes, allowing later seral species to increase. Blowout penstemon populations and plant size declined in the 1990s when precipitation was higher than normal, resulting in reduced sand movement. We conducted a greenhouse experiment to determine whether blowing sand influenced vigor and persistence of blowout penstemon seedlings. Treatments were wind, sandblasting, wind with sandblasting, and a control. The wind treatment was a constant 14 km h^{–1 …}

Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F₂ and F₃ plants and F_3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …

Three Essays On The Role Of Amenities As An Economic Development Strategy, Junpyo Hong

LSU Doctoral Dissertations

It is well known that an amenity is a key driving engine to regional economic growth. However, the site-specific nature of an amenity can characterize them as public goods. Due to this characteristic, local governments have difficulty optimally supplying amenities. This dissertation tries to find relationships between an amenity and economic growth. Three empirical papers comprise the original research in this dissertation. The findings of the meta-analysis in the first essay suggest little methodological diversity exists among researchers linking amenities to economic growth., I do find that employment growth is more likely related to man-made amenities even in research on …

Evaluation And Enhancement Of Seed Lot Quality In Eastern Gamagrass [Tripsacum Dactyloides (L.) L.], Cynthia Hensley Finneseth

University of Kentucky Doctoral Dissertations

Eastern gamagrass [Tripsacum dactyloides (L.) L.] is a warm-season, perennial grass which is native to large areas across North America. Cultivars, selections and ecotypes suitable for erosion control, wildlife planting, ornamental, forage and biofuel applications are commercially available. Plantings are commonly established from seed; however, seed quality and dormancy are barriers to establishment. The objective here was to investigate parameters that contribute to inconsistent performance of this seed kind to subsequently improve seed lot quality.

Forty-two seed lots were used, with seed fill and germination potential ranging from 78- 100% and 11-91%, respectively. Actual germination and dormant seed ranged …

Synopsis Of The "First 100 Years Of Agricultural Education In North Carolina", North Carolina Agricultural & Technical State University

Documents

Synopsis of the "First 100 Years of Agricultural Education in North Carolina," FNSF and NFA mentioned on pg. 2, S. B. Simmons Camp on pg. 4, A. L. Teachey, W. T. Johnson and C. E. Dean listed as State Leaders in Agriculture Education.

Growth And Yield Of Cultivated Grape With Native Perennial Grasses Nodding Needlegrass Or California Barley As Cover Crops, Michael J. Costello

Wine and Viticulture

Two California native perennial grasses, nodding needlegrass [Nassella cernua (Stebbins & R.M. Love) Barkworth] and California barley [Hordeum brachyantherum Nevski ssp. californicum (Covas & Stebbins) Bothmer, N. Jacobsen & Seberg], were compared with a conventional grass cover crop, ‘Blando’ brome (Bromus hordeaceus L.), as well as resident (weedy) vegetation and a clean cultivated control for effects on growth and yield of cultivated grape (Vitis vinifera L. cv. Barbera). Statistical analyses did not reveal yield differences between treatments with floor vegetation (the native grasses, ‘Blando’ brome, and resident vegetation) and clean cultivation, the cover crop treatments (the native grasses and ‘Blando …

Training Livestock To Leave Streams And Use Uplands, Usu Extension

All Current Publications

Cattle can damage streams and surrounding vegetation—riparian areas—by breaking down banks decreasing water quality, and reducing wildlife living in the stream and on the land.

Strawberry Plug Plant Production, Dan Drost, Brent Black, Daniel Rowley

All Current Publications

This publication provides information on growing strawberries including planting dates and harvesting methods.

Visible Near-Infrared Hyperspectral Imaging For The Identification And Discrimination Of Brown Blotch Disease On Mushroom (Agaricus Bisporus) Caps, Edurne Gaston, Jesus Maria Frias, Patrick J. Cullen, Colm O’Donnell, Aoife Gowen

Articles

Brown blotch, caused by pathogenic Pseudomonas tolaasii (P. tolaasii), is the most problematic bacterial disease in Agaricus bisporus mushrooms. Although it does not cause any health problems, it reduces the consumer appeal of mushrooms in the market place, generating important economical losses worldwide. Hyperspectral imaging (HSI) is a non-destructive technique that combines imaging and spectroscopy to obtain information from a sample. The objective of this study was to investigate the use of HSI for brown blotch identification and discrimination from mechanical damage on mushrooms. Hyperspectral images of mushrooms subjected to i) no treatment, ii) mechanical damage or iii) …

Action Research, Knowledge & Impact: Experiences Of The Global Livestock Crsp Pastoral Risk Management Project In The Southern Ethiopian Rangelands, D. Layne Coppock

Environment and Society Faculty Publications

No abstract provided.