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Comparison Of Plant‐Adapted Rhabdovirus Protein Localization And Interactions, Kathleen Marie Martin 2011 University of Kentucky

Comparison Of Plant‐Adapted Rhabdovirus Protein Localization And Interactions, Kathleen Marie Martin

University of Kentucky Doctoral Dissertations

Sonchus yellow net virus (SYNV), Potato yellow dwarf virus (PYDV) and Lettuce Necrotic yellows virus (LNYV) are members of the Rhabdoviridae family that infect plants. SYNV and PYDV are Nucleorhabdoviruses that replicate in the nuclei of infected cells and LNYV is a Cytorhabdovirus that replicates in the cytoplasm. LNYV and SYNV share a similar genome organization with a gene order of Nucleoprotein (N), Phosphoprotein (P), putative movement protein (Mv), Matrix protein (M), Glycoprotein (G) and Polymerase protein (L). PYDV contains an additional predicted gene between N and P, denoted as X, that has an unknown function. In order to gain …


2011 Weed Science Research Report – Gold Section, NDSU Weed Science, Dept. of Plant Sciences 2011 North Dakota State University

2011 Weed Science Research Report – Gold Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2011. It includes work on sugarbeet and the management of several weeds in North Dakota.


2011 Weed Science Research Report – Pink Section, NDSU Weed Science, Dept. of Plant Sciences 2011 North Dakota State University

2011 Weed Science Research Report – Pink Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2011. It includes work on leafy spurge, Russian olive and yellow toadflax in North Dakota.


2011 Weed Science Research Report – Yellow Section, NDSU Weed Science, Dept. of Plant Sciences 2011 North Dakota State University

2011 Weed Science Research Report – Yellow Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2011. It includes work on several  minor crops and the management of several weeds in North Dakota.


Examining Adminitrators' Disciplinary Philosophies: A Conceptual Model, Brittany Wilkinson Smith 2011 University of Kentucky

Examining Adminitrators' Disciplinary Philosophies: A Conceptual Model, Brittany Wilkinson Smith

University of Kentucky Master's Theses

In the 40th Annual Phi Delta Kappa/Gallup Poll of the Public’s Attitudes toward the Public Schools, Americans rated discipline as the second largest problem facing public education (Bushaw & Gallup, 2008). This poses a substantial problem for administrators as they strive to employ school reform policies, address public demands and meet the needs of contemporary students. A review of literature revealed a large body of research which examines disciplinary practice; however, it also showcased a literary gap regarding administrative disciplinary philosophies. This multiple case study highlights disciplinary philosophies possessed by five secondary school administrators. Analysis included utilizing the Administrator Discipline …


Switchgrass Contains Two Cinnamyl Alcohol Dehydrogenases Involved In Lignin Formation, Aaron J. Saathoff, Christian M. Tobias, Scott E. Sattler, Eric J. Haas, Paul Twigg, Gautam Sarath 2011 USDA-ARS

Switchgrass Contains Two Cinnamyl Alcohol Dehydrogenases Involved In Lignin Formation, Aaron J. Saathoff, Christian M. Tobias, Scott E. Sattler, Eric J. Haas, Paul Twigg, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Lignin content of switchgrass (Panicum virgatum L.), a bioenergy species, is a critical determinant of biomass quality since it can negatively impact conversion of biomass into liquid fuels via biochemical platforms. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin biosynthesis. Here, we have shown that cv. Kanlow switchgrass contains at least two closely related CAD genes (PviCAD1 and PviCAD2) that code for proteins containing highly conserved domains and residues that identify them as bona fide CADs. Both recombinant proteins displayed substrate kinetics consistent with their presumed role in cell wall lignification. Proteomic and immunoblotting detected …


The Composition And Origins Of Genomic Variation Among Individuals Of The Soybean Reference Cultivar Williams 82, William J. Haun, D. L. Hyten, Wayne W. Xu, Daniel J. Gerhardt, Thomas J. Albert, Todd Richmond, Jeffrey A. Jeddeloh, Gaofeng Jia, Nathan M. Springer, Carroll P. Vance, Robert M. Stupar 2011 University of Minnesota

The Composition And Origins Of Genomic Variation Among Individuals Of The Soybean Reference Cultivar Williams 82, William J. Haun, D. L. Hyten, Wayne W. Xu, Daniel J. Gerhardt, Thomas J. Albert, Todd Richmond, Jeffrey A. Jeddeloh, Gaofeng Jia, Nathan M. Springer, Carroll P. Vance, Robert M. Stupar

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max) is a self-pollinating species that has relatively low nucleotide polymorphism rates compared with other crop species. Despite the low rate of nucleotide polymorphisms, a wide range of heritable phenotypic variation exists. There is even evidence for heritable phenotypic variation among individuals within some cultivars. Williams 82, the soybean cultivar used to produce the reference genome sequence, was derived from backcrossing a Phytophthora root rot resistance locus from the donor parent Kingwa into the recurrent parent Williams. To explore the genetic basis of intracultivar variation, we investigated the nucleotide, structural, and gene content variation of different …


Genome-Wide Association Analysis Identifies Candidate Genes Associated With Iron Deficiency Chlorosis In Soybean, Sujan Mamidi, Shireen Chikara, R. Jay Goos, D. L. Hyten, Deepti Annam, Samira Mafi Moghaddam, Rian K. Lee, P. B. Cregan, Phillip E. McClean 2011 North Dakota State University

Genome-Wide Association Analysis Identifies Candidate Genes Associated With Iron Deficiency Chlorosis In Soybean, Sujan Mamidi, Shireen Chikara, R. Jay Goos, D. L. Hyten, Deepti Annam, Samira Mafi Moghaddam, Rian K. Lee, P. B. Cregan, Phillip E. Mcclean

Department of Agronomy and Horticulture: Faculty Publications

Iron deficiency chlorosis (IDC) is a significant yield-limiting problem in several major soybean [Glycine max (L.) Merr.] production regions in the United States. Soybean plants display a variety of symptoms that range from a slight yellowing of the leaf to interveinal chlorosis, to stunted growth that reduces yield. The objective of this analysis was to employ single nucleotide polymorphism (SNP)-based genome-wide association mapping to uncover genomic regions associated with IDC tolerance. Two populations [2005 (n = 143) and 2006 (n = 141)] were evaluated in replicated, multilocation IDC trials. After controlling for population structure and individual relatedness, and selecting …


The Role Of Transition Metal Homeostasis In Plant Seed Development, Elsbeth L. Walker, Brian M. Waters 2011 University of Massachusetts Amherst

The Role Of Transition Metal Homeostasis In Plant Seed Development, Elsbeth L. Walker, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Copyright © 2011 Elsevier Ltd. Used by permission.


Moving Magnesium In Plant Cells, Brian M. Waters 2011 University of Nebraska-Lincoln

Moving Magnesium In Plant Cells, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Magnesium (Mg) is among the most abundant mineral elements in plants, yet the knowledge of which genes control its accumulation in specific tissues and organelles lags behind that of many other mineral elements. Only in recent years has identification of important molecular players begun to take shape. In this issue of New Phytologist, Conn et al. (pp. 583–594) shed additional light on two Mg transporters that play important roles in accumulation of Mg in leaf cell vacuoles. Using subcellular-level ion measurements on leaves, gene expression measurements after single-cell sampling, a genetic approach, and clever use of calcium (Ca) and …


Letter From The Dean, Michael Vayda 2011 University of Arkansas, Fayetteville

Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


A Novel Gene Silencing Vector For Plant Genomics And Biotechnology, Dustin Hoover, Scott Nicholson, Vibha Srivastava 2011 University of Arkansas, Fayetteville

A Novel Gene Silencing Vector For Plant Genomics And Biotechnology, Dustin Hoover, Scott Nicholson, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene silencing is a process of suppressing activity of specific genes by producing “interfering” RNA encoded by foreign genes. This process serves as the principle of genetic modification in plants and animals, which is an important tool in genomics and biotechnology, allowing scientists to manipulate organisms to better meet human demands. New approaches of gene silencing may enable improvements on current practices of genetic modification, and broaden the application and impact of gene silencing in biotechnology. Recently, a novel vector design consisting of the transcription of short gene fragments lacking transcription termination signals was demonstrated to be effective in partial …


Instructions For Authors, Discovery Editors 2011 University of Arkansas, Fayetteville

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Monoclonal Antibodies Bind A Snp-Sensitive Epitope That Is Present Uniquely In Mycobacterium Avium Subspecies Paratuberculosis, John Bannantine, Judith R. Stabel, Elise A. Lamont, Robert E. Briggs, Srinand Sreevatsan 2011 USDA ARS National Animal Disease Center

Monoclonal Antibodies Bind A Snp-Sensitive Epitope That Is Present Uniquely In Mycobacterium Avium Subspecies Paratuberculosis, John Bannantine, Judith R. Stabel, Elise A. Lamont, Robert E. Briggs, Srinand Sreevatsan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Due to a close genetic relatedness, there is no known antibody that detects Mycobacterium avium subspecies paratuberculosis (MAP), which causes Johne’s disease in cattle and sheep, and does not cross-react with other M. avium subspecies. In the present study, a monoclonal antibody (MAb; 17A12) was identified from mice immunized with a cell membrane fraction of MAP strain K-10. This antibody is 100% specific as it detected a 25-kDa protein in all 29 MAP whole cell lysates, but did not bind to any of the 29 non- paratuberculosis strains tested in immunoblot assays. However, the antibody revealed variable reactivity levels in …


Ultimate Biochemical Oxygen Demand In Semi-Intensively Managed Shrimp Pond Waters, Bartholomew Green, George H. Ward 2011 Stuttgart National Aquaculture Research Center

Ultimate Biochemical Oxygen Demand In Semi-Intensively Managed Shrimp Pond Waters, Bartholomew Green, George H. Ward

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Three independent studies were conducted to quantify ultimate biochemical oxygen demand (UBOD) and the corresponding decomposition rate constant for production pond (average 21.5 ha each) waters and effluents on six semi-intensively managed marine shrimp (Litopenaeus vannamei) farms in Honduras. Samples were collected during the rainy season in studies 1–3 and during the dry season only in Study 1. Pond water samples were collected during the culture period and/or at harvest. The dry season 30-d biochemical oxygen demand (BOD30) of pond waters across all farms was 26% lower and UBOD was 54% lower, but the decomposition rate constant was …


Growing South Dakota (Winter 2011), College of Agriculture &. Biological Sciences 2011 South Dakota State University

Growing South Dakota (Winter 2011), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Introducing iGrow: New Web Portal Brings Information To One Location
[Page] 4 Crops, Cattle & Much More: Opportunities Farm Cultivates Research, Teaching And Scholarships
[Page] 7 College News
[Page] 8 Funding Fundamentals: Budget Cuts Have Far-Reaching Impact
[Page] 10 The Value Extension: Three Producers Share How They've Benefited [Page] 12 A Vision for Precision: Gregg Carlson Has Helped Guide Agronomy
[Page] 13 A Boost for Beginning Beef Producers: Training Program Offered Through USDA Grant
[Page] 14 Ethanol's Evolution: Promising New Research Goes Beyond Corn
[Page] 16 New Era for Cereal Grains: Seed Technology Lab Inspires Innovation
[Page] 18 …


Achieving Real Development Impact Among Pastoralists: Lessons From Ethiopia, D. Layne Coppock 2011 Utah State University

Achieving Real Development Impact Among Pastoralists: Lessons From Ethiopia, D. Layne Coppock

Environment and Society Faculty Publications

No abstract provided.


Character Education And 4-H Youth Development, Jessica Lynn Mullins 2011 University of Kentucky

Character Education And 4-H Youth Development, Jessica Lynn Mullins

University of Kentucky Master's Theses

Character education assumes responsibility for influencing and educating youth on morals, values, beliefs and positive character attributes in order to influence them into being positive and contributing members of society. Character education is most often taught in the home, schools and community. One community organization that integrates character education into its programming is 4-H Youth Development. The purpose of this research is to view the relationships between character education and 4-H, and more so whether Kentucky 4-H programs influence the knowledge, attitudes and practices of positive character among youth leaders. This study also looked at length of 4-H involvement and …


Restoration Of Tall Fescue Pastures To Native Warm Season Grasslands: Does A Fungal Endophyte Symbiosis Play A Role In Restoration Success?, Sarah Lynn Hall 2011 University of Kentucky

Restoration Of Tall Fescue Pastures To Native Warm Season Grasslands: Does A Fungal Endophyte Symbiosis Play A Role In Restoration Success?, Sarah Lynn Hall

University of Kentucky Doctoral Dissertations

Tall fescue, a cool-season grass native to Europe, central Asia, and northern Africa, has been widely distributed throughout the U.S. for use as turf and forage. Following its widespread planting, its ability to associate with a toxic fungal endophyte, Neotyphodium coenophialum, was discovered. Research has linked this fescue-endophyte association with increased biotic and abiotic stress resistance in endophyte-infected (E+) versus endophyte-free (E-) plants, and these differences may affect the ability of land managers to eradicate tall fescue and restore native grasslands. I conducted three studies to examine whether E+ tall fescue plants respond differently to management than E- plants, …


Intermediate Steps Of Loline Alkaloid Biosynthesis, Jerome Ralph Faulkner 2011 University of Kentucky

Intermediate Steps Of Loline Alkaloid Biosynthesis, Jerome Ralph Faulkner

University of Kentucky Doctoral Dissertations

Epichloë species and their anamorphs, Neotyphodium species, are fungal endophytes that inhabit cool-season grasses and often produce bioprotective alkaloids. These alkaloids include lolines, which are insecticidal and insect feeding deterrents. Lolines are exo-1-aminopyrrolizidines with an oxygen bridge between carbons 2 and 7, and are usually methylated and formylated or acetylated on the 1-amine. In previously published studies lolines were shown to be derived from the amino acids L-proline and L-homoserine. In addition the gene cluster involved in loline-alkaloid biosynthesis has also been characterized. In this dissertation a survey of plant-endophyte symbioses revealed a phenotype with only N-acetylnorloline. This …


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