Agriculture Commons^™

Changing Land Use On Unproductive Soils, Andrew Blake, Mike Clarke, Angela Stuart-Street

Resource management technical reports

A three year study completed in 2010 examined farming systems in the north-eastern agricultural region and eastern wheatbelt of Western Australia which experienced a succession of variable seasons from 2000. This culminated in severe drought in 2006 and 2007. At that time, farmers experienced labour shortages and declining terms of trade triggered by cost inflation outpacing growth in commodity prices. As a result, farm business equity was eroded as farm debt escalated. This left many farm businesses in a highly vulnerable state. At the time of the study many farmers were facing an uncertain future.

These experiences, coupled with a …

Identification And Characterization Of A Spore-Like Morphotype In Chronically Starved Mycobacterium Avium Subsp. Paratuberculosis Cultures, Elise A. Lamont, J. P. Bannantine, Anibal Armien, Don Sanjiv Ariyakumar, Suelee Robbe-Austerman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacteria are able to enter into a state of non-replication or dormancy, which may result in their chronic persistence in soil, aquatic environments, and permissive hosts. Stresses such as nutrient deprivation and hypoxia provide environmental cues to enter a persistent state; however, a clear definition of the mechanism that mycobacteria employ to achieve this remains elusive. While the concept of sporulation in mycobacteria is not novel, it continues to spark controversy and challenges our perceptions of a non-replication. We investigated the potential role of sporulation in one-year old broth cultures of Mycobacterium subsp. paratuberculosis (MAP). We show that dormant cultures …

Evaluation Of Protection In A Mouse Model After Vaccination With Mycobacterium Avium Subsp. Paratuberculois Protein Cocktails, J.R. Stabel, A. Barnhill, J. P. Bannantine, Y.F. Chang, M.A. Osman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Whole-cell vaccines successfully reduce signs of clinical disease and fecal shedding of Mycobacterium avium subsp. paratuberculosis (MAP), however, these vaccines have some limitations. The present study was conducted to identify MAP proteins that might be candidates for the development of an improved vaccine. MAP proteins were screened for immunogenicity in naturally infected cattle and selected based upon reactivity in the interferon-􏰀 (IFN-􏰀) and Western blot assays. Proteins (MAP1087, MAP1204, MAP1272c, and MAP2077c) were arrayed into 4 overlapping cocktails containing 3 proteins each. The efficacy of the proteins within these cocktails as vaccine candidates was evaluated by subcutaneous immunization of mice, …

2012 Weed Science Research Report – Blue Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2012. It includes work on corn, soybean and the management of several weeds in North Dakota.

2012 Weed Science Research Report – Gold Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2012. It includes work on sugarbeet and the management of several weeds in North Dakota.

2012 Weed Science Research Report – Tan Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2012. It includes work on adjuvants and droplet size in North Dakota.

2012 Weed Science Research Report – Yellow Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2012. It includes work on several minor crops and the management of several weeds in North Dakota.

2012 Weed Science Research Report – White Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document provides environmental, indexes, abbreviations and collaborators in 2012.

Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang

Department of Entomology: Faculty Publications

Transgenic corn (Zea mays L.) products expressing multiple Bt proteins targeting a same group of insect pests have become commercially available in the U.S. The first commercialized pyramided Bt corn technologies for managing lepidopteran pests include Genuity® VT Triple Pro™ and Genuity® SmartStax™. In this study, larval survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and -heterozygous (Cry1Ab-RS) genotypes of the sugarcane borer, Diatraea saccharalis F., on five commercial corn hybrids were evaluated in a leaf tissue bioassay and two greenhouse trials during 2010–2011. The five hybrids included two non-Bt corn and three Bt corn hybrids representing three …

Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard

Department of Entomology: Faculty Publications

Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) due to feeding on MON863 transgenic maize (Zea mays L.) expressing the Cry3Bb1 protein was evaluated at three Missouri sites in both 2003 and 2004 and at one site each in South Dakota, Nebraska, and Iowa in 2004. To do this, survivorship relative to survivorship on isoline maize (i.e., the same genetic background, but without Cry3Bb1) was evaluated. Comparisons were made using low (1650–2500 eggs/m) and high (3300–3500 eggs/m) western corn rootworm egg densities. Significantly fewer beetles were recovered from MON863 than from isoline maize. Emergence from MON863 as …

Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu

Department of Entomology: Faculty Publications

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) …

Using Video-Tracking To Assess Sublethal Effects Of Pesticides On Honey Bees (Apis Mellifera L.), Bethany S. Teeters, Reed M. Johnson, Marion D. Ellis, Blair D. Siegfried

Department of Entomology: Faculty Publications

Concern about the role of pesticides in honey bee decline has highlighted the need to examine the effects of sublethal exposure on bee behaviors. The video-tracking system EthoVisionXT (Noldus Information Technologies) was used to measure the effects of sublethal exposure to tau-fluvalinate and imidacloprid on honey bee locomotion, interactions, and time spent near a food source over a 24-h observation period. Bees were either treated topically with 0.3, 1.5, and 3 μg tau-fluvalinate or exposed to 0.05, 0.5, 5.0, 50, and 500 ppb imidacloprid in a sugar agar cube. Tau-fluvalinate caused a significant reduction in distance moved at all dose …

Pheromone–Mediated Mating Disruption In The Millet Stem Borer, Coniesta Ignefusalis (Lepidoptera: Pyralidae), Ousmane Youm, Yacouba Maliki, David R. Hall, Dudley I. Farman, John E. Foster

Department of Entomology: Faculty Publications

The millet stem borer, Coniesta ignefusalis Hampson (Lepidoptera: Pyralidae), is a major pest of pearl millet in the Sahelian region of Africa. The female sex pheromone has been identified and synthesized, and previous research had shown that the synthetic pheromone could cause high levels of reproductive communication disruption in small plots when released at rates of 640 mg/ha/day, using PVC resin formulation renewed every seven days to maintain efficiency. In the present research, in experiments in farmers’ fields in Niger, 86.8% (SE = 2.6%) communication disruption was achieved when polyethylene vials loaded with 0.5 mg pheromone at 400 dispensers/ha were …

Visual Communications On The Road In Arkansas: Analysis Of Secondary Students Videos, Stuart Estes, Kristin M. Pennington, Leslie D. Edgar

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

In the summer of 2010, the Visual Communications on the Road in Arkansas: Creative Photo and Video Projects to Promote Agriculture program was initiated. The program consisted of a two-week agricultural communications curriculum that would be taught by agricultural science teachers in Arkansas. The curriculum was composed of lessons about photography, writing, and videography, and the program introduced students to digital photography and videography equipment and the proper uses of equipment. Once the curriculum was taught in secondary schools, a mobile classroom unit—consisting of a travel trailer, photography and videography equipment, and laptop computers equipped with editing software—would visit the …

Enantioselective Demethylation: The Key To The Nornicotine Enantiomeric Composition In Tobacco Leaf, Bin Cai

Theses and Dissertations--Plant and Soil Sciences

Nicotine and nornicotine are the two main alkaloids that accumulate in Nicotiana tabacum L. (tobacco), and nornicotine is the N-demethylation metabolite of nicotine. Nicotine is synthesized in the root, and probably primarily in the root tip. Both nicotine and nornicotine exist as two isomers that differ from each other by the orientation of H atom at the C-2' position on the pyrrolidine ring. (S)-nicotine is the dominant form in tobacco leaf and the enantiomer fraction of nicotine (EF_nic), the fraction of (R)-enantiomer over the total nicotine, is approximately 0.002. Despite considerable efforts to elucidate nicotine and nornicotine …

Method Development For Detecting And Characterizing Manufactured Silver Nanoparticles In Soil Pore Water Using Asymmetrical Flow Field-Flow Fractionation, Annie R. Whitley

Theses and Dissertations--Plant and Soil Sciences

Recent advances in nanotechnology have led to the production of materials with nanoscale dimensions (nm) and properties distinctly different from their bulk (>100 nm) counterparts. With increased use, it is inevitable that nanomaterials will accumulate in the environment and there is concern that the novel properties of nanomaterials could result in detrimental environmental and human health effects. In particular, there has been concern recently regarding the use of silver (Ag) based nanomaterials as antimicrobial agents in consumer and medical products. Current regulations dealing with the discharge of metals into the environment are based on total concentrations with no consideration …

Immunogenicity And Protective Efficacy Of The Mycobacterium Avium Subsp. Paratuberculosis Attenuated Mutants Against Challenge In A Mouse Model, Jenn-Wei Chen, Syed M. Faisal, Subhash Chandra, Sean P. Mcdonough, Maria A.S. Moreira, Joy Scaria, Chao-Fu Chang, J. P. Bannantine, Bruce Akey, Yung-Fu Chang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Johne’s disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), results in serious economic losses worldwide especially in cattle, sheep and goats. To control the impact of JD on the animal industry, an effective vaccine with minimal adverse effects is urgently required. In order to develop an effective vaccine, we used allelic exchange to construct three mutant MAP strains, leuD, mpt64 and secA2. The mutants were attenuated in a murine model and induced cytokine responses in J774A.1 cell. The leuD mutant was the most obviously attenuated of the three constructed mutant strains. Our preliminary vaccine trial in mice demonstrated different …

Perspectives On The Use Of Grounded Theory In Social Development Studies, Ernesto L. Bumatay

Journal of Public Affairs and Development

This methodology paper introduces insights and perspectives on Grounded Theory (GT) as a social science research methodology. While GT emanates from the field of education, this paper shows that it can also be potentially used in development studies. The concept of the GT is first explained including the alternative views on its implementation. It has limited application in the Philippine context, and the current application that is cited is based on an education study called “Becoming Gift: A Classic Grounded Theory of Charism in Sectarian Institutions”. The end goal of the paper is to argue for the application of GT …

Theoretical Concepts And Practice Of Community Organizing, Josefina T. Dizon

Journal of Public Affairs and Development

Community organizing, one of the methods in community development, is a Western concept adopted in the Philippines. The different definitions of foreign and local authors highlight the nature of community organizing as a process by which a community identifies its problems and finds solutions through collective mobilization of the people and resources. This paper discusses the theoretical concepts of community organizing, which include its ideological background and value orientations, assumptions and propositions, goals, approaches, and principles. Towards the end, the paper discusses the steps involved in community organizing and forwards a conceptual framework of community organizing focused on people empowerment …

Transdisciplinary Approach In Development Studies, Agnes C. Rola

Journal of Public Affairs and Development

This paper traces the origin, evolution, and the propositions made by the framers of the transdisciplinary (TD) concept. It reviews the body of work of the various applications of the TD approach in the human sciences as in the twinning of medical sciences and the social sciences; biodiversity; agriculture sciences and development; and agriculture, food, and nutrition. Another application of the TD approach is discussed in the context of innovation systems for development studies with focus on sustainable community development and food security. The experiences of the College of Public Affairs and Development (CPAf), University of the Philippines Los Baños …