Agriculture Commons^™

Use Of Gene Editing In Usda Research, Mark R. Mclellan, Patsy Brannon, Adriana Campa, Carrie Castille, Steven Daley-Laursen, Roch Gaussoin, Govind Kannan, Mark Lawrence, Dawn Thilmany

Biology Faculty Publications and Presentations

The Science Advisory Council was established in FY2016 as a subcommittee of the National Agricultural Research, Extension, Education, and Economics (NAREEE) Advisory Board by the Chief Scientist as a result of recommendations from the 2012 report on Agricultural Preparedness by the President’s Council of Advisors on Science and Technology. The Council's charge is to provide advice and guidance, on a scientific basis, on the overall strength, practicality, and direction of agricultural research, including emerging technology and scientific issues and report any findings publicly to the NAREEE Advisory Board.

The USDA Chief Scientist first asked the Council to examine rigor and …

Study On Antioxidation Stability Of Collagen Peptides Prepared From Swimming Bladders, Zheng Tingting, Tu Zongcai, Tang Pingping, Zhang Lu, Sha Xiaomei, Wang Hui

Food and Machinery

The change of scavenging activity on DPPH radical, hydroxyl free radical, and reducing power capacity was selected as the index to investigate the effects of pH, temperature, sucrose, preservatives, metal ions and gastrointestinal digestion in vitro on the antioxidant stability of collagen peptides from swimming bladders. The results showed that collagen peptides from swimming bladders kept high activity in acidity environment but lose it in alkaline condition; however, the strong heat-resistance was found; sucrose, NaCl and preservatives (sodium Benzoate and Sodium sorbate) had little effects on its antioxidation stability, but high levels of salt made it lost antioxidation stability partially. …

The Effects Of Different Processing Methods On Expected Glycemic Index Of Cereals And Legumes, Cui Yanan, Zhang Hui, Wang Li, Qian Haifeng, Qi Xiguang

Food and Machinery

The effects of different processing methods (steaming, extruding and roller-drying) on eGI of adlay, oat, rye, colored kidney bean and chickpea were investigated. The results showed that the eGI of steamed samples were in the following order: rye＞adlay＞oat＞ colored kidney bean＞chickpea. The eGI of extruded samples were in the following order: adlay＞rye＞oat＞colored kidney bean＞chickpea. The eGI of roller-dried samples were in the following order:adlay＞rye＞oat＞colored kidney bean＞chickpea. By different processing methods, the eGI of oat, colored kidney bean and chickpea were lower among five samples. The eGI of the same kind of cereal or legume after streaming, extruding and roller-drying were …

A New And Simple Method For Calculating The Inclusion Ratio Of Cyclodextrin Supramolecules, Zhang Weifeng, Luo Xin, Wang Jianhui, Wang Min, Liu Long, Wang Chengfeng, Ning Jingheng

Food and Machinery

Based on UV absorbance, a new and simple subtraction method was proposed for the calculation of the inclusion ratio, and it was proved to be equal in number to the classical molar ratio or phase solubility method. Using any kind of these three methods, the results always showed that the inclusion ratio of this complex was 11, which indicated that this kind of supramolecular complex was formed through an inclusion interaction between 1 host molecule (β-cyclodextrin) and 1 guest molecule (sorbic acid). Sincethe subtraction methodadopted a repetitive measurement and took the averaged, its result was more accurate than the molar …

Effects Of Exogenous Oxidation Stress On Glutathione Synthesis In Saccharomyces Cerevisiae Mutant Y518, Zheng Lixue, Qi Bin, Sun Jiang, Wang Limei

Food and Machinery

Effects of exogenous oxidative stress on glutathione production by Saccharomyces cerevisiae mutant Y518 were researched by addition of exogenous hydrogen peroxide. The results showed that the addition of hydrogen peroxide inhibited the growth of Y518 cells, but promoted the synthesis of glutathione of Y518. The best addition time and amount of hydrogen peroxide were respectively 24 h and 0.6 mmol/L. The content of gluconate reached (13.69±0.28) mg/g, increased by about 14% compared to that without hydrogen peroxide. Exogenous oxidation stress on Y518 was produced by adding 0.6 mmol/L hydrogen peroxide at 24 h, which activated the transcription regulation factor YAP1 …

Degradation Kinetics And Transformation Mechanism Research Of Hydroxy-Α-Sanshool From Zanthoxylum Bungeanum Exposed To Uv Radiation, Yan Yali, Liu Fuquan, Zhao Zhifeng, Gu Xuequan, Xing Yong

Food and Machinery

To study the variation trend of hydroxy-α-sanshool from Zanthoxylum bungeanum exposed to UV radiation and its transformation direction, the hydroxy-α-sanshool, a typical alkylamides of Zanthoxylum bungeanum, was extracted, separated and purified from Zanthoxylum oil and exposed to UV radiation. The alkylamides content was detected by using HPLC after different hours of radiation. The results indicated that hydroxy-α-sanshool under UV radiation decreased rapidly by 95.9% in 8 hours. Furthermore, the concentration and time obeyed the first order reaction rate equation C=490.68e^{-0.346 9t}, meanwhile it transformed into hydroxy-ε-sanshool, which was 8.56 times of its initial content after 3 hours. Moreover, …

Probiotic Properties Of Potato Resistant Starches Modified By A Combination Of Organic Acid And Heat-Moisture Treatments, Liu Yang, Li Xiaowen, Zhu Hong, Xie Tao, Yi Cuiping

Food and Machinery

Some potato resistant starches (AH-HMT RS3) were made by using acid hydrolysis combined with heat-moisture treatment (AH-HMT), and the effects of different types and concentration of organic acids and the starch slurry concentration on the formation of slowly digestive starch (SDS) and resistant starch (RS) in potato RS3 were investigated. The results demonstrated that the starch slurry concentrations had no effect on the formation of SDS and RS when the potato starches were modified by no acid combined with heat-moisture treatment (HMT). Compared with no acid-HMT, the RS contents in potato AH-HMT RS3 made by AH-HMT increased 21.89%～45.09%, non-influenced by …

Effects Of Γ-Polyglutamic Acid On Functional Properties Of Chicken Myofibrillar Protein, Bai Dengrong, Dong Wei, Qi Xinyu, Shang Yongbiao

Food and Machinery

Different concentration of γ-polyglutamic acid were added to chicken myofibrillar protein, and the effects of γ-polyglutamic acid on the function of chicken myofibrillar protein were studied. The study found that with the increase of the concentration of γ-polyglutamic acid, surface hydrophobicity of myofibrillar protein first decreases and then increases, and reached the minimum when the concentration of γ-polyglutamic acid was 0.6‰; the solubility, emulsification, the gelatin of hardness, springiness and water holding capacity were first increases and then decreases, and respectively reached the maximum when the concentration of γ-polyglutamic acid was 0.6‰, but γ-polyglutamic acid on gelatin whiteness had little …

Effects Of Baking Degrees On Quality Characteristics Of Wuyi Rock Tea, Zhang Lei, Lin Yanqing, Luo Liyong, Zeng Liang

Food and Machinery

Wuyi rock teas made from Shuixian or Rougui tea cultivar were baked for different degrees. Their organoleptic properties and principal biochemical components, and the physical properties of tea infusion (aberration, transmittance, precipitation amount and particle size) were determined in this study, to evaluated the effect of different baking technics on the quality of Wuyi rock tea. The results showed that baking process were beneficial to the improvement of Wuyi rock tea quality. The contents of tea polyphenols, caffeine, free amino acids, theafavins and thearubigins significantly decreased with increasing baking degrees. However, there were no significant variations in the contents of …

Comparison Of Oil Characteristics Between Camellia Osmantha And Other Camellia Species, Chen Bolin, Zhong Haiyan, Li Guiqing, Zhu Xiaoyang, Long Qizhi

Food and Machinery

Camellia osmantha is a new species found in Guangxi Province. In this study the oil quality was measured compared with other camellia species in respect of iodine value, saponification value, fatty acid, triglyceride composition and other attributes. Acid value, peroxide value, iodine value and saponification value of tested samples ranged as 0.38～1.47 mg/g, 2.15～4.56 mmol/kg, 79.39～85.86 g/100 g and 187.97～196.37 mg/g, respectively. The acid value and iodine value of C. osmantha were 0.42 mg/g and 2.98 mmol/kg that reached Grand one of Oil-tea camellia seed oil standard. Thirteen kinds of triglycerides were detected and the fatty acid contents were as …

Composition And Positional Distribution Of Fatty Acids In Triacylglycerols Of Three Marine Fish Oils, Zhang Huijun, Wang Xingguo, Jin Qinzhe

Food and Machinery

In this study, the purified triacylglycerols (TAG) of selected marine fish oils (anchovy, tuna and salmon oils) were subjected to stereospecific hydrolysis by an sn-1, 3-specific lipase (Lipozyme RM IM) which cleaves selectively the sn-1 and sn-3 position ester bonds of TAG yielding to sn-2-monoglycerides (2-MAG). After lipase hydrolysis, the released free fatty acids and sn-2-MAG were isolated by thin-layer chromatography (TLC). The original TAG and sn-2-MAG were respectively converted to fatty acid methyl esters (FAME), then analyzed by gas chromatography(GC). Results showed that TAG of these marine fish oils were made up of unsaturated fatty acids mainly accounting for …

Study On Thermophysical Properties Of Tobacco And Smoke Agent Under Different Temperature, Ma Yaping, Liu Chaoxian, Wang Le, Zhang Mingjian, Zhang Ke, Deng Nan, Lu Duanfeng, Tang Jianguo, Liu Xiangzhen

Food and Machinery

The thermophysical properties of tobacco and smoke agent (the mass ratio of glycerol and propylene glycol is 51) was measured by transient plane heat source method, and the effects of moisture and smoke agent content on reconstituted cut tobacco and flue-cured were measured. The results showed that with the increase of temperature (22～75 ℃), thermal conductivity \[0.06～0.12 W/(m·K) and 0.06～0.16 W/(m·K\] and volume heat capacity \[0.17～2.70 MJ/(m³K) and 0.15～1.45 MJ/(m³K)\] increased; however, the thermal diffusivity decreased (0.33～0.05 mm² / s and 0.40～0.09 mm² / s, respectively). With the increase of moisture content (0%～13%) , …

Spectrophotometric Determination Of Chromium Content In Beer Yeast With Safranine T, Li Renyu, Huang Jinzhuang, Li Yongmei, Zhou Jiahong

Food and Machinery

A new spectrophotometric method for determination of chromium content was developed. In both polyvinyl alcohol and phosphoric acid medium, chromium (VI) could react with potassium iodide and safranine T at room temperature, leading to coloration after microwave digestion sample and oxidation of chromium (III) to chromium (VI) with potassium permanganate under alkaline conditions. The effects of the factors such as absorption spectra, reaction conditions and coexisting ions on the reaction were discussed. The results showed that under the optimum determination conditions, Beer-Lambert law was obeyed in the mass concentration range of 0.0～0.5 μg/mL for chromium with the maximum absorption peak …

Detect The Content Of Polar Components In Frying Oils By Preparative Flash Chromatography, Xue Bin, Cao Wenming, Yin Yujie

Food and Machinery

A fast method of separating and detecting the polar compounds in frying oils by preparative FLASH chromatography was established in this study. Applying the EOPC type medium pressure liquid preparative chromatography system as hardware platform and EOPC-specified FLASH column as separation & preparation column, petroleum etherdiethyl ether(8713) as elution solvent, 25 min/min as flow speed, we found polar components(PCs) and non-polar components(non-PCs) in frying oils could be separated properly when elution time of non-PCs was 11min. Separation speed was fast, as the time took by preparative FLASH chromatography was only 41 min, repeatability RSDs of preparative FLASH chromatography were no …

Design Of Grain Moisture Detection Device, Xu Baowen, Cai Jianrong, Sun Li, Zhang Wensong, An Yuting, He Zhenlu

Food and Machinery

A set of grain moisture detection device based on incident microwave was designed, and the STC89C52 chip was used as the main processor to control the execution order of each component. Moreover, the photoelectric switch was used to keep the amount of the loading material in line. In order to eliminate the influence of temperature, it was detected in real time, and the temperature compensation model is established. To avoid the thermal effect of microwave, the sequential control system was applied to collect temperature and microwave signals, ensuring the accurate measurement of grain moisture. The results showed that the correlation …

Dynamic Analysis Of High Speed Cam Mechanism With Clearance Based On Impact Model, He Xueming, He Kai, Wu Meiping, Ji Xiaogang

Food and Machinery

In order to improve the dynamic performance of cam mechanism with high clearance. The two-state impact sub-model is used to simulate the gap between the cam and the follower to construct the dynamic model of the gripping cam mechanism considering the damping. The finite difference method is used to solve the dynamic equation with the cycloidal cam curve as the excitation function. The time domain and frequency domain analysis of the gap dynamics model are carried out, and the corresponding acceleration time domain and frequency domain response map are drawn. The influence of the damping ratio and the cycle ratio …

Analysis Of Dynamics For Vibrating Body Of Vibrating Loose Machine Excited By Single Vibrating Motor, Deng Chengjiang, Zhao Yin, Zhao Yunfei, Zhang Xianlian

Food and Machinery

The vibration loose machine was designed to pre-loose the strips being cut. It was beneficial to increase the moisture regain of the stem. The Single Vibrating Motor was placed transversely on the vibrating body as an excitation source of the vibration loose machine. The differential equation of its vibration was established based on analyzing the structure of the vibrating body when the rubber elastic component was regarded as a rigid body. The motion equation and trajectory of the vibrating body could be obtained in more details. The vibration characteristics were analyzed comparatively by the two kinds of calculation method. The …

Analysis Of Internal Flow Field And Optimization Of Mechanism Parameters Of Negative Pressure Electric Heating Dryer Based On Fluent, Liu Daoqi, Fan Chuanhui, Dong Huifeng, Li He

Food and Machinery

The problems of uneven flow field and low drying efficiency in the negative pressure electric heating dryer were found and supposed to solve in this study. Based on Fluent, numerical simulation of wind velocity field and temperature field in negative pressure electric heating dryer was carried out. And by using the improved design of SolidWorks internal structure of the dryer, the main factors influencing the drying efficiency of the working parameter and structure parameter were tested. By using changing the working parameters and optimize the structure, the internal flow field uniformity of the optimal effect of dryer were achieved. The …

Study On Separation Performance Of An Ultra-High-Gravity Mini-Hydrocyclone For Starch Production, Yu Jianfeng, Fu Jian, Xie Yaocong, Zheng Xiangyang, Zhao Jiang

Food and Machinery

A novel ultra-high-gravity mini-hydrocyclone was designed. One-factor experiments and two-factor experiments were conducted to study the effects of the feed flow rate, split ratio, feed concentration and vortex finder diameter on separation performance of rice starch and potato starch, respectively. The results indicated that the effects of the feed flow rate, split ratio and feed concentration on the total separation efficiency for rice and potato starch were remarkable in the one-factor experiments; the feed flow rate, split ratio and vortex finder diameter significantly affected the total separation efficiency in the two-factor experiments. The interaction effects between the feed flow rate …

Schematic Design And Simulation Study On Alkaline Noodle Loosening Device, Yin Qiang, Yang Chuang, Peng Hui, Zhang Guoquan, Li Xiangfa

Food and Machinery

Alkaline noodle loosening device of automated production was designed, and the overall scheme was then researched. It was carried out the research on the structure design of roller with multiple stirring rod, wheel and bracket, conveyor belt and so on, and its parameters were given. Alkaline noodle loosening device waas modeled by using Adams software. The rationality of the design was verified through kinematics simulation from the two aspects such as rotary speed and angular acceleration of the roller. The contact force of the conveyor belt and contact force of the roller were tested through dynamic simulation, providing the reference …