Veterinary Medicine Commons^™

Using Handmade Cloning To Reduce Oocyte Mitochondria In Reconstructed Embryos, Laura Adams

Student Research Symposium

Mitochondrial heteroplasmy, the presence of more than one population of mitochondrial DNA (mtDNA) in one cell, can cause metabolic issues and reduced ATP synthesis, even at relatively low levels. The presence of this condition in somatic cell nuclear transfer (SCNT) embryos likely contributes to reduced prenatal and postnatal viability: the majority of the oocyte’s mitochondria is still present at the time of nuclear transfer, as well as through any further embryonic development post-fusion and activation. Though the somatic cell also contributes its mitochondria (which can successfully communicate with its nucleus), the oocyte’s mitochondria compose a greater proportion of the total …

Roles Of Nad+ In Testicular Aging And Epigenetic Sperm Modifications, Renata Hoskova

Student Research Symposium

Since 1980, the average age of first-time fathers has been increasing. With increased age, sperm chromatin quality and nicotinamide adenine dinucleotide (NAD) levels are reduced. In order to condense around protamines, sperm DNA undergoes breaks, at which time epigenetic reprogramming occurs. In humans, about 2% of DNA stays wound to histones in spermatozoa. Histones carry post-translational modifications that control gene activity and have the potential to transmit epigenetic information to the offspring. Analysis of epigenetic sperm modifications is being conducted within niacin deficient versus niacin replete mice. The experimental plan includes the characterization of histone exchange in elongating spermatids via …

Fecal Microbiota Transfer (Fmt) From Tumor-Bearing Mice Fed The Total Western Diet (Twd) Does Not Contribute To A Higher Tumor Burden In Mice Fed A Healthy Diet, Porter Green

Student Research Symposium

Colorectal cancer (CRC) is the third most common cause of cancer death in the United States. Diet and gut microbiome have shown to influence the development of colitis associated colorectal cancer (CAC). For this study, the goal was to determine the association between colon inflammation, diet and microbiome in the development of CAC in mice. The primary objective of this study was to determine the contribution of gut microbiota from mice donors who have previously consumed one of two basal diets: 1) the standard AIN93G diet, which is designed to promote rodent health; and 2) the total Western diet (TWD), …

Roles Of Nad In Dna Repair And Response To Oxidative Stress, Micah Forbush

Student Research Symposium

It has become a common trend for people to start their families at a later age, often due to lifestyle choices like achieving career goals first and waiting to achieve financial stability. Along with this trend of increasing parental age comes another, the decrease in fertility. While it is well established that female fertility declines significantly with age, current research is showing that male fertility may be impacted just as much. NAD (Nicotinamide Adenine Dinucleotide) is an important biochemical cofactor in most metabolic reactions, and importantly, is it also essential for DNA repair. As age increases in humans, the amount …

Bolstering Southeast Asia's Livestock Biosecurity And Climate Change Resilience Essential For Global Health, Madelyn Kunzler

Student Research Symposium

A large factor of climate change is its effect on infectious zoonotic diseases. Zoonotic diseases are diseases that can be transferred from animals into humans. Cases of zoonotic diseases have increased as humans have interacted more with animals, and as worldwide travel has become more frequent. It is predicted that 60% of emerging human diseases will be zoonotic. The cases will continue to increase to due climate change. As climate change continues, research has shown that new diseases will emerge from the melting permafrost and ice caps. It is also likely that pre-existing diseases will be able to migrate to …

Differentiation Of The Can F 1 Allergen In Hypoallergenic Dog Saliva Compared To Shedding Dog Saliva, Rose Miller

Honors College Theses

Dog saliva contains a large variety of proteins, each with specific functions throughout the body. While some aid in digestion, others provide immune support for the body. In the case of human dog allergies, dog skin dander has been proven to cause allergic reactions to those who are sensitive to the allergens in the dogs. However, past studies have shown a difference in allergic reaction to specific breeds, breaking dog breeds into two main categories: hypoallergenic and non-hypoallergenic. Within these groups, there are different hypoallergenicity levels, but overall, the hypoallergenic dog group does not cause an allergic reaction, whereas non-hypoallergenic, …

Anesthetic Combination Of Alfaxalone, Dexmedetomidine And Hydromorphone For Comparison Of Endoscopy-Guided Intubation And Blind Orotracheal Intubation In Domestic Rabbits (Oryctolagus Cuniculus), Stephanie Reabel

LSU Master's Theses

Domestic rabbits have a high risk of anesthesia-related mortality compared to other companion animals. Factors that may contribute to increased mortality include drug-induced cardiopulmonary effects (Grint & Murison 2008; Navarrete-Calvo et al. 2013), difficulty performing intubation (Aeschbacher 1995; Flecknell 2016; Wenger et al. 2017) or damage to laryngeal and tracheal tissues following orotracheal intubation (Grint et al. 2006; Phaneuf et al. 2006; Engbers et al. 2017).

Alfaxalone is a neuroactive steroid that produces induction of anesthesia and cardiorespiratory depression in a dose-dependent manner when injected intravenously. The ability to induce anesthesia via an intramuscular (IM) route has several advantages in …

Associations Among Stress, Anxiety, Depression, And Emotional Intelligence Among Veterinary Medicine Students, Julia Wells, Kylie Watson, Robert E. Davis, Syed Siraj A. Quadri, Joshua R. Mann, Ashutosh Verma, Manoj Sharma, Vinayak K. Nahar

Environmental & Global Health Faculty Research

Background: Veterinary students are faced with immense pressures and rigors during school. These pressures have contributed to elevated levels of stress, anxiety, and depression (SAD) among veterinary students relative to the general population. One proposed concept to help students combat SAD is that of emotional intelligence (EI). We explored the relationship between EI and SAD among veterinary students at a college in the Southeast United States. Methods: A crosssectional study design was implemented among a convenience sample of 182 veterinary medical students. The survey instrument contained 56 items that elicited information about students’ demographics, perceived stress, anxiety, and depression, and …

Using Enclosed Y-Mazes To Assess Chemosensory Behavior In Reptiles, M. Rockwell Parker, Andrea F. Currylow, Eric A. Tillman, Charlotte J. Robinson, Jilian M. Josimovich, Isabella M.G. Bukovich, Lauren A. Nazarian, Melia G. Nafus, Bryan M. Kluever, Amy A. Yackel Adams

United States Department of Agriculture Wildlife Services: Staff Publications

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and …

Effects Of Strong Oxidants Present In Acer Spp. On Hemolysis Methemoglobin Production In Equine Erythrocytes, Gabrielle Mcgeorge, Jeffrey Lehman

Undergraduate Honors Thesis Projects

Maple toxicosis is a deadly condition affecting equines after they have ingested wilted leaves of the genus Acer (i.e., maple species). It is characterized by hemolytic anemia and methemoglobinemia. Although the toxins responsible for causing toxicosis have not been identified, they are thought to be strong oxidants. The objective of this study is to determine the effect of eight compounds (galloyl derivatives) found in Acer spp. on hemolysis and methemoglobin production in bioassays with equine erythrocytes. Seven of the compounds are known constituents of Acer spp. that are commercially available. One of the compounds--acertannin--was isolated and characterized from leaf extracts …

Effects Of Cryopreservation, Immunophenotype And Sex On Multipotentiality, Cell Proliferation And Gene Expression In Feline Adipose-Derived Stromal Cells, Maia Williams

Honors Capstones

No abstract provided.

Unprecedented Migratory Bird Die-Off: A Citizen-Based Analysis On The Spatiotemporal Patterns Of Mass Mortality Events In The Western United States, Di Yang, Anni Yang, Jue Yang, Rongting Xu, Han Qiu

United States Department of Agriculture Wildlife Services: Staff Publications

Extensive, severe wildfires, and wildfire-induced smoke occurred across the western and central United States since August 2020. Wildfires resulting in the loss of habitats and emission of particulate matter and volatile organic compounds pose serious threatens to wildlife and human populations, especially for avian species, the respiratory system of which are sensitive to air pollutions. At the same time, the extreme weather (e.g., snowstorms) in late summer may also impact bird migration by cutting off their food supply and promoting their migration before they were physiologically ready. In this study, we investigated the environmental drivers of massive bird die-offs by …

Growing South Dakota (Spring 2021), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2020 SDSU Extension Annual Report and 2020 South Dakota Agricultural Experiment Station Annual Report.

[Page] 3 COVID-19 Impacts
[Page] 9 Precision Agriculture: What's New
[Page] 13 Featured Research
[Page] 19 SDSU Extension 2020 Annual Report
[Page] 45 South Dakota Agricultural Experiment Station 2020 Annual Report
[Page] 55 CAFES News and Updates
[Page] 67 Alumni News
[Page] 75 Alumni Gone Down in History
[Page] 81 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Determination Of The Presence Of Rickettsia Spp. And Borrelia Spp. Carried By Tortoise Ticks From Madagascar, Anna Phan

Biological Sciences Theses & Dissertations

Ticks were removed from three species of Malagasy tortoises, Astrochelys yniphora, A. radiata, and Pyxis arachnoides (comprising two subspecies P. a. arachnoides and P. a. oblonga), between 2012 and 2015. The ticks were presumed to be from the genus Amblyomma. Ticks were morphologically identified and then checked molecularly to confirm their classification or identify any ticks that could not be morphologically identified. Molecular identification was done via end-point PCR that amplified tick cytochrome oxidase (CO1) and tick 12S rRNA genes. Ticks were screened via a real-time polymerase chain reaction assay for the presence of Rickettsia spp. …

Determining The Effects Of Exogenous Human Chorionic Gonadotropin And Pregnant Mare Serum Gonadotropin On Plasma Testosterone, Testicular Volume, And Semen Production In Leopard Geckos (Eublepharis Macularius), Alexandra Mason

LSU Master's Theses

Planet earth has now entered into its sixth mass extinction as a result of expanding human populations, posing an unprecedented threat to biologic diversity. Climate change, habitat fragmentation, wildlife trade, pollution, and biological invasions are just a few consequences of human activities that have directly contributed to unprecedented species extinctions. While widespread amphibian declines have gained more recent attention, it has been proposed that reptiles may be in even greater danger of extinction worldwide. Unfortunately, many reptiles are destined to go extinct before many of their basic biologic traits, including reproductive methods, have even been determined.

In order to mitigate …

An Inactivated Bacterium (Paraprobiotic) Expressing Bacillus Thuringiensis Cry5b As A Therapeutic For Ascaris And Parascaris Spp. Infections In Large Animals, Joseph F. Urban, Martin K. Nielsen, David Gazzola, Yue Xie, Ethiopia Beshah, Yan Hu, Hanchen Li, Florentina Rus, Kelly Flanagan, Austin Draper, Sridhar Vakalapudi, Robert W. Li, Gary R. Ostroff, Raffi V. Aroian

Maxwell H. Gluck Equine Research Center Faculty Publications

Ascaris and Parascaris are important parasites in the family Ascarididae, large, ubiquitous intestinal-dwelling nematodes infecting all classes of vertebrates. Parasitic nematode drug resistance in veterinary medicine and drug recalcitrance in human medicine are increasing worldwide, with few if any new therapeutic classes on the horizon. Some of these parasites are zoonotic, e.g., Ascaris is passed from humans to pigs and vice versa. The development of new therapies against this family of parasites would have major implications for both human and livestock health. Here we tested the therapeutic ability of a paraprobiotic or dead probiotic that expresses the Bacillus …

“Adopt-A-Tissue” Initiative Advances Efforts To Identify Tissue-Specific Histone Marks In The Mare, N. B. Kingsley, Natasha A. Hamilton, Gabriella Lindgren, Ludovic Orlando, Ernest Bailey, Samantha Brooks, Molly Mccue, Theodore S. Kalbfleisch, James N. Macleod, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Long Non-Coding Rna Zfas1 Is A Major Regulator Of Epithelial-Mesenchymal Transition Through Mir-200/Zeb1/E-Cadherin, Vimentin Signaling In Colon Adenocarcinoma, Stephen J. O'Brien, Casey Fiechter, James Burton, Jacob Hallion, Mason Paas, Ankur Patel, Ajay Patel, Andre Rochet, Katharina Scheurlen, Sarah Gardner, Maurice Eichenberger, Harshini Sarojini, Sudhir Srivastava, Shesh Rai, Theodore S. Kalbfleisch, Hiram C. Polk, Susan Galandiuk

Veterinary Science Faculty Publications

Colon adenocarcinoma is a common cause of cancer-related deaths worldwide. Epithelial-mesenchymal transition is a major regulator of cancer metastasis, and increased understanding of this process is essential to improve patient outcomes. Long non-coding RNA (lncRNA) are important regulators of carcinogenesis. To identify lncRNAs associated with colon carcinogenesis, we performed an exploratory differential gene expression analysis comparing paired colon adenocarcinoma and normal colon epithelium using an RNA-sequencing data set. This analysis identified lncRNA ZFAS1 as significantly increased in colon cancer compared to normal colon epithelium. This finding was validated in an institutional cohort using laser capture microdissection. ZFAS1 was also found …

Characterization Of Memory T-Lymphocyte Subsets In Dogs, Annie Beth Bauman

LSU Master's Theses

A more complete understanding of canine T-cell immunity is necessary for improving diagnostic and therapeutic approaches to canine diseases, developing cell-based canine immunotherapeutics, and evaluating dogs as large mammal models for comparative immunology research. Canine immunological studies are limited by the lack of established phenotypic markers identifying diverse differentiation states in canine T-cells. Differential expression of CD45RA (indicating antigen experience) and CD62L (indicating lymph node homing capability), shows promise as a tool to delineate canine naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector memory (TEMRA) T-cells. The aim of this study was to characterize canine memory T-cell …

Maldi-Tof Ms Biomarker Detection Models To Distinguish Rtx Toxin Phenotypes Of Moraxella Bovoculi Strains Are Enhanced Using Calcium Chloride Supplemented Agar, Matthew M. Hille, Michael L. Clawson, Aaron M. Dickey, Justin H. Lowery, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the bacterium most often cultured from ocular lesions of cattle with infectious bovine keratoconjunctivitis, also known as bovine pinkeye. Some strains of M. bovoculi contain operons encoding for a repeats-in-toxin (RTX) toxin, which is a known virulence factor of multiple veterinary pathogens. We explored the utility of MALDI-TOF MS and biomarker detection models to classify the presence or absence of an RTX phenotype in M. bovoculi. Ninety strains that had undergone whole genome sequencing were classified by the presence or absence of complete RTX operons and confirmed with a visual assessment of hemolysis on blood agar. Strains …

Pioneer Science And The Great Plagues: How Microbes, War, And Public Health Shaped Animal Health, Norman F. Cheville, Purdue University Press

Purdue University Press Books

Pioneer Science and the Great Plagues covers the century when infectious plagues—anthrax, tuberculosis, tetanus, plague, smallpox, and polio—were conquered, and details the important role that veterinary scientists played. The narrative is driven by astonishing events that centered on animal disease: the influenza pandemic of 1872, discovery of the causes of anthrax and tuberculosis in the 1880s, conquest of Texas cattle fever and then yellow fever, German anthrax attacks on the United States during World War I, the tuberculin war of 1931, Japanese biological warfare in the 1940s, and today’s bioterror dangers.

Veterinary science in the rural Midwest arose from agriculture, …

Generation Of A Biobank From Two Adult Thoroughbred Stallions For The Functional Annotation Of Animal Genomes Initiative, Callum G. Donnelly, Rebecca R. Bellone, Erin N. Hales, Annee Nguyen, Scott A. Katzman, Ghislaine A. Dujovne, Kelly E. Knickelbein, Felipe Avila, Theodore S. Kalbfleisch, Elena Giulotto, Nicole B. Kingsley, Jocelyn Tanaka, Elizabeth Esdaile, Sichong Peng, Anna Dahlgren, Anna Fuller, Michael J. Mienaltowski, Terje Raudsepp, Verena K. Affolter, Jessica L. Petersen, Carrie J. Finno

Maxwell H. Gluck Equine Research Center Faculty Publications

Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic …

Sars-Cov-2 Exposure In Escaped Mink, Utah, Usa, Susan A. Shriner, Jeremy W. Ellis, J. Jeffrey Root, Annette Roug, Scott R. Stopak, Gerald W. Wiscomb, Jared R. Zierenberg, Hon S. Ip, Mia Kim Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

In August 2020, outbreaks of coronavirus disease were confirmed on mink farms in Utah, USA. We surveyed mammals captured on and around farms for evidence of infection or exposure. Free-ranging mink, presumed domestic escapees, exhibited high antibody titers, suggesting a potential severe acute respiratory syndrome coronavirus 2 transmission pathway to native wildlife.

We report a wildlife epidemiologic investigation of mammals captured on or near properties in Utah, USA, where outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurred in farmed mink. Mink farms are relatively common in the United States, and most are small family farms. The US …

The Effects Of Mechanical Rocking Motion On Transdifferentiating Feline Adipose Multipotent Stromal Cells Into Functioning Pancreatic Β-Cell Like Islets, Emily Halphen

Honors Capstones

No abstract provided.

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level …

Avian Visual Ecologists Should Consider Uv Absorbance And All Sensory Modalities: A Response To Wisenden Et Al. (2020), Sean T. O'Daniels, Scott J. Werner, Ken Yasukawa

United States Department of Agriculture Wildlife Services: Staff Publications

In a recent publication, Wisenden et al. (2020) examined responses of territorial male Red-winged Blackbirds (Agelaius phoeniceus) to models constructed with ultraviolet (UV)-reflective red epaulets for the purpose of determining if the addition of UV reflectance to epaulets ("UV+") changed the effectiveness of signals to receivers relative to "control" epaulets under field conditions. The authors hypothesized that "UV+epaulet coloration represents a visual signal with increased efficacy in territorial interactions." They presented behavioral data but no visual modeling data. Our aims in this commentary are to suggest alternative terms to those used by the authors, to express concern about the use …

Clinical Evaluation Of The Accuracy Of Foetal Radiographic Findings In Parturition Date Prediction Of Small Size Dogs, Panthipa Borikappakul, Nardtiwa Chaivoravitsakul, Rampaipat Penchome, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Ultrasonographic measurements of extra-foetal or foetal structures have been reported as an accurate means of predicting the date of parturition in the bitch. However, for many years, radiography has been clinically useful for pregnancy diagnosis, determining the number of foetuses and prolonged foetal death. Guidelines for estimating the canine parturition date based on foetal skeletal mineralization in relation to gestational age and preovulatory LH surge have been applied in small animal practice. The aims of this retrospective study were to evaluate the accuracy of parturition date prediction using foetal radiographic findings in small size dogs (1-10 kg), and to determine …

Pharmacokinetics Of Oxfendazole Nanosuspension In Sheep, Yuzhu Sun, Xiaofang Wang, Aoxue Zhang, Bao Zhang, Liwei Shao, Yue Liu, Haiting Wang, Wanhe Luo, Dongmei Chen, Shuyu Xie

The Thai Journal of Veterinary Medicine

The pharmacokinetics of our previous prepared oxfendazole nanosuspension was studied in sheep to understand its enhancement of bioavailability. Ten sheep were randomly divided into two groups, each of which received a singledose (5mg/kg) oral oxfendazole nanosuspension and oxfendazole granules. After intragastric administration, oxfendazole rapidly reached peak concentrations of 11.25 μg/mL and 5.50 µg/mL at 5.6 h and 6.6 h in the nanosuspension group and granules group and the concentrations gradually reduced to below the detection limit at 96 h and 72h, respectively. The main pharmacokinetic parameters of oxfendazole after administration to the sheep: The Tmax, Cmax, AUClast, MRTlast, T1/2 and …

Evaluation Of Dermal Toxicity Study Of Ethanolic Extract Of Morinda Citrifolia Fruit In Spraque Dawley Rats, Farhan Hanif Reduan Mohd, Rosly Shaari Mohd, Nurul Syahirah Ahmad Sayuti, Abdullahi Aliyu, Noordin Mohamed Mustapha, Md Zuki Abu Bakar, Shanmugavelu Sithambaram, Hazilawati Hamzah

The Thai Journal of Veterinary Medicine

Plant-based preparations are widely known to complement dermatological therapy, however toxic profiles of these plants are lacking and limited. To investigate the toxicity of Morinda citrifolia fruit ethanol extract applied to the skin of rats daily for 28 days, thirty female Sprague Dawley rats were grouped into five groups (n=5 rats for each group) for each study. The experimental period was set at 14 days and 28 days for acute and subacute dermal toxicity, respectively. Rats in the treatment groups were topically applied with the plant extract at three different doses; 2.5%, 5% and 10% on a shaved area of …

Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan

The Thai Journal of Veterinary Medicine

Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …