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2021

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Articles 541 - 556 of 556

Full-Text Articles in Veterinary Medicine

Genetic Characterization And Strain Differentiation Of Feline Calicivirus Among Cat Population In Thailand By High Resolution Melting Analysis, Kannika Phongroop Jan 2021

Genetic Characterization And Strain Differentiation Of Feline Calicivirus Among Cat Population In Thailand By High Resolution Melting Analysis, Kannika Phongroop

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline calicivirus (FCV) is wildly known as one of the significant causative viral pathogens causing self-limited upper respiratory tract and oral disease in cats. In the last decade, severe FCV form, as named a virulent systemic feline calicivirus (VS-FCV), has distinctly emerged worldwide even though commercially available vaccines for FCV are typically used in Thai cats. However, the data of either prevalence or molecular characterization has never been officially noticed in Thailand. Thus, this study aimed to provide molecular epidemiology and characterization of the FCV circulating in Thai cats during 2016-2021. Moreover, the high-resolution melting analysis (HRM) is utilized for …

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Developing A Detection Method For Rabies Virus Neutralizing Antibodies Using Pseudotype Technique, Nichapat Rojjananavin Jan 2021

Developing A Detection Method For Rabies Virus Neutralizing Antibodies Using Pseudotype Technique, Nichapat Rojjananavin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rabies is a neuro-fatal disease, causing by rabies virus (RABV) infection. Two serological tests, recommended by the World Health Organization and the World Organization for Animal Health, namely the rapid fluorescent focus inhibition test (RFFIT) and the fluorescent antibody virus neutralization test (FAVN) are considered gold standard. However, as both the RFFIT and FAVN require the use of live viruses, they raise biosafety concerns. Moreover, the immunostaining step in both methods is costly and time-consuming. In this study, RABV-pseudotype was developed and used in a RVNA detection method. The RABV-pseudotype based on lentivirus gave higher titer than the vesicular stomatitis …

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Roles Of Balb/C Mice And Aedes Mosquitoes In The Transmission Of Duck Tembusu Virus, Nichapat Yurayart Jan 2021

Roles Of Balb/C Mice And Aedes Mosquitoes In The Transmission Of Duck Tembusu Virus, Nichapat Yurayart

Chulalongkorn University Theses and Dissertations (Chula ETD)

Duck Tembusu virus (DTMUV) is an emerging flavivirus that causes severe nervous and systemic diseases in avian hosts. The virus has been classified into three clusters, and the predominant cluster in Thailand is cluster 2.1. The pathogenesis of the virus has been extensively studied in avian hosts but not in mammalian hosts. Therefore, the first objective of this dissertation was to investigate the viral pathogenesis in mammalian hosts. Six-week-old BALB/c mice were intracerebrally and subcutaneously inoculated with Thai DTMUV to examine clinical signs, pathological change, viral load, and virus distribution. Results demonstrated that Thai DTMUV caused an acute severe disease, …

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A Serological And Molecular Survey Of Sars-Cov-2 Infection In Domestic Dogs And Cats In Bangkok And Vicinity Thailand, Kitikhun Udom Jan 2021

A Serological And Molecular Survey Of Sars-Cov-2 Infection In Domestic Dogs And Cats In Bangkok And Vicinity Thailand, Kitikhun Udom

Chulalongkorn University Theses and Dissertations (Chula ETD)

The COVID-19 pandemic caused by SARS-CoV-2 has significant impact to human health worldwide. As of June 2022, over 500 million human cases with 6 million deaths have been reported. Despite SARS-CoV-2 infection in human, SARS-CoV-2 infections in domestic animals have also been reported due to spillover of the viruses from human-animals interface. The aim of this study was to conduct serological and molecular detection of SARS-CoV-2 in dogs and cats in Bangkok and vicinity in Thailand during the pandemic outbreaks. A large-scale SARS-CoV-2 survey in domestic animals which a total of 4,696 serum samples (2,960 of dogs, 1,736 of cats) …

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Detection Of Antimicrobial Resistance In Escherichia Coli And Salmonella Spp. Originated From Cultivated Oysters And Estuarine Waters, Mullika Kuldee Jan 2021

Detection Of Antimicrobial Resistance In Escherichia Coli And Salmonella Spp. Originated From Cultivated Oysters And Estuarine Waters, Mullika Kuldee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The raising and spreading antimicrobial resistance (AMR) bacteria in humans, animals, and environment is a serious global public health issue. Improper use of antimicrobial agents can promote resistant bacteria, and as a result, they can circulate in the environment. Contamination of AMR in the environment can increase the risk of AMR distribution in humans, animals, and the environment. Monitoring and surveillance of AMR in the environmental sector are limited compared to animal and human sectors. The objectives of this study were to determine phenotype and genotype of AMR and extended-spectrum beta-lactamase (ESBL) production in Escherichia coli and Salmonella isolated from …

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Study Of Aerotolerant Campylobacter Jejuni In Broiler Production Process, Nichanan Jarusirivait Jan 2021

Study Of Aerotolerant Campylobacter Jejuni In Broiler Production Process, Nichanan Jarusirivait

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter jejuni is the leading cause of human gastroenteritis worldwide. Although they are microaerophile and sensitive to oxygen, aerotolerant C. jejuni has been reported in many countries. Currently, limited information on aerotolerant C. jejuni in Thailand has been reported. Therefore, the objectives of this study were 1) to examine the occurrence of aerotolerant C. jejuni in farms, slaughterhouses and retail markets and compare the level of aerotolerant strain between different sample types at the farm (i.e., cloacal swabs VS manures) and slaughterhouse level (i.e., cecal contents VS carcass rinses) and 2) to identify common genotypes of aerotolerant and aerosensitive C. …

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Knowledge, Attitude And Practice Associated With Brucellosis In Occupational Risk Groups In China, Quangang Xu Jan 2021

Knowledge, Attitude And Practice Associated With Brucellosis In Occupational Risk Groups In China, Quangang Xu

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to analyze the situation of knowledge, attitude, and practice (KAP) of sheep and goat farmers and community animal health staff towards the prevention and control of brucellosis. A total of 1,067 sheep and goat farmers and 401 community animal health staff from seven provinces in China were participated in this study from 2017 to 2018. Two structured questionnaire were used to examine demographic characteristics and KAP related to brucellosis. Descriptive statistics were used for demographic characteristics and KAP. Mann-Whitney U test was used to analyze the potential risk factors associated with KAP among participants. The overall awareness …

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Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter, Sirawich Danwanichwong Jan 2021

Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter, Sirawich Danwanichwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter is one of the most common causes of bacterial foodborne disease in humans worldwide. The effect of probiotics on Campylobacter control in poultry is inconsistent or rarely observed. For this reason, research on probiotics for Campylobacter control in poultry is required. Therefore, the objective of this study was to identify lactobacilli, Bacillus or Enterococcus strains isolated from Campylobacter-negative chickens which have the ability to inhibit the growth of Campylobacter in vitro and demonstrate favorable probiotic characteristics. A total of 602 bacterial isolates from Campylobacter-negative broiler chickens were assessed for their probiotic properties including Campylobacter inhibiting activity test, hemolytic activity …

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In Vitro Inhibitory Effect Of Lactic Acid Bacteria And Their Supernatants On Flavobacterium Columnare Biofilm, Farras Daffa Imtiyaz Jan 2021

In Vitro Inhibitory Effect Of Lactic Acid Bacteria And Their Supernatants On Flavobacterium Columnare Biofilm, Farras Daffa Imtiyaz

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is an opportunistic bacterium causing columnaris disease to more than 37 fish species. It is believed that the bacteria form biofilm on the fish skin/surfaces before the bacteria in the biofilm produce and release enzymes that degrade connective and other tissues resulting in lesions on the infected fish. Treatments to inhibit the bacterial biofilm without interfering with its planktonic cell growth can be an alternative method to control columnaris disease. This study aimed to evaluate the efficacy of lactic acid bacteria (LAB) isolated from white leg shrimp gut (Penaeus vannamei) and the root of Jerusalem artichoke (Helianthus tuberosus) …

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Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak Jan 2021

Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Foodborne pathogens and antimicrobial resistant bacteria in tilapia and cultivation water has been emerged as a public health threat. This study aimed to determine the prevalence of indicator and pathogenic bacteria in hybrid red tilapia (Oreochromis spp.) and their cultivation water, and to characterize phenotypic and genotypic AMR, virulence genes, and extended-spectrum beta-lactamase (ESBL) production of Escherichia coli, Aeromonas hydrophila, Salmonella spp., and Vibrio cholerae. A total of 120 tilapia, which were comprised of carcass rinse (n=120), muscle (n=120), intestine (n=120), liver and kidney (n=120), and cultivation water (n=120) were collected from tilapia farms in Kanchanaburi province, Thailand during October …

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Genetic Characterizations, Virulence, Antimicrobial Resistances And Resistome Analysis Of Vibrio Spp. Isolated From Farmed Asian Sea Bass (Lates Calcarifer) In Thailand, Hartanto Mulyo Raharjo Jan 2021

Genetic Characterizations, Virulence, Antimicrobial Resistances And Resistome Analysis Of Vibrio Spp. Isolated From Farmed Asian Sea Bass (Lates Calcarifer) In Thailand, Hartanto Mulyo Raharjo

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study describes the etiological agent of Vibriosis along with its distribution and antimicrobial resistance profiles among farmed Asian sea bass (Lates calcarifer) in Thailand. The study isolated 283 Vibrionaceae from 15 Asian sea bass farms located around the provinces of the Andaman Sea and Gulf of Thailand coasts to uncover the distribution and antimicrobial resistance profiles. Bacterial identification based on a combination of the biochemical characteristics, Matrix-assisted laser desorption/ ionization-time of flight (MALDI-TOF) mass spectrometry (MS) analysis, and the species-specific PCR demonstrated the predominant Vibrionaceae were Vibrio harveyi (n = 56), Photobacterium damselae (n = 35), and V. vulnificus …

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Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah Jan 2021

Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah

Chulalongkorn University Theses and Dissertations (Chula ETD)

Striped snakehead fish (Channa striata) is one of the popular cultivated fish in Thailand. However, it is inevitable to be susceptible to abundant pathogens, especially Aeromonas schubertii. In July 2020, an unknown outbreak was occurred on striped snakehead fish nursery farm in Nakhon Pathom province, Thailand. Suspected six isolates were A. schubertii yielded from the fish as the main causative agent. This research aimed to characterize and examine pathogenicity of A. schubertii isolated from diseased striped snakehead fish. Characterization was performed based on combination of biochemical assay, PCR and DNA sequencing (based on 16S rRNA and gyrB), and MALDI-TOF MS. …

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Genome Analysis Of Predominant Aeromonas Species Isolated From Diseased Freshwater Fishes And Their Virulence In Striped Snakehead Fish (Channa Striata)||การวิเคราะห์จีโนมเชื้อแอโรโมนาสสปีชีส์เด่นที่แยกได้จากปลาน้ำจืดป่วยในประเทศไทยและความรุนแรงของเชื้อในปลาช่อนนา, Muhammad Fadhlullah Mursalim Jan 2021

Genome Analysis Of Predominant Aeromonas Species Isolated From Diseased Freshwater Fishes And Their Virulence In Striped Snakehead Fish (Channa Striata)||การวิเคราะห์จีโนมเชื้อแอโรโมนาสสปีชีส์เด่นที่แยกได้จากปลาน้ำจืดป่วยในประเทศไทยและความรุนแรงของเชื้อในปลาช่อนนา, Muhammad Fadhlullah Mursalim

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas is an important bacterial pathogen in freshwater fishes. This study aimed to investigate the distribution and diversity of Aeromonas spp. including their antimicrobial resistance patterns. 86 Aeromonas isolates were collected from diseased fish in 13 fish farms around Thailand. Biochemical tests, MALDI-TOF MS, PCR assays, and the gyrB gene sequence analysis were used to identify all isolates. MALDI-TOF MS confirmed 100% (86 isolates) at the genus level and 88.4% (76 isolates) at the species level. Six Aeromonas species were confirmed based on sequences of gyrB gene, including A. veronii (72.1%), A. jandaei (11.6%), A. schubertii (9.3%), A. diversa (3.5%), …

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Monitoring And Characterization Of Colistin Resistant Escherichia Coli In Pig Farm And Environment Following The Cessation In Use Of Colistin, Nwai Oo Khine Jan 2021

Monitoring And Characterization Of Colistin Resistant Escherichia Coli In Pig Farm And Environment Following The Cessation In Use Of Colistin, Nwai Oo Khine

Chulalongkorn University Theses and Dissertations (Chula ETD)

The growing cases of colistin resistance due to plasmid mediated mcr genes family in Enterobacteriaceae is catastrophic to public health. In this study, our team performed the nation-wide surveillance of mcr-positive Escherichia coli (MCRPE) in healthy pigs across Thailand. Then we monitored longitudinally over the representative pig farm after colistin was withdrawn and genomic characterization was carried out. Among the 696 samples collected from 49 provinces of Thailand, the low carriage rate of mcr-1 or combination of mcr-1 and mcr-3 (4.45% and 0.43%) were detected. MCRPE isolates were multidrug-resistant (MDR) against 3–14 types of antimicrobial and enterotoxin genes were largely …

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Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath Jan 2021

Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia is the world's second most important farmed fish species, following carp. Bangladesh is the world's fourth-largest tilapia producer and has largely adopted fish polyculture. Few research on factors associated with mortality and economic losses following such deaths have been undertaken in Bangladesh due of its perceived hardiness. Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV could be widespread, we surveyed 565 tilapia farms in 15 of Bangladesh's most important tilapia-producing districts using online tilapia epidemiology and health economics survey …

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Anti-Inflammatory Efficacy Of New Zealand Green-Lipped Mussel Extract In Canine Chronic Stifle Osteoarthritis Monitoring By Thermographic Imaging, Supakit Vilaisuntornkiat Jan 2021

Anti-Inflammatory Efficacy Of New Zealand Green-Lipped Mussel Extract In Canine Chronic Stifle Osteoarthritis Monitoring By Thermographic Imaging, Supakit Vilaisuntornkiat

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the anti-inflammatory effect of the NZGLM and placebo in patients with cranial cruciate ligament disease using thermographic imaging. This study evaluated the temperature from thermographic imaging to measure the reduce of inflammation. This study included 52 dogs with cranial cruciate disease and medial patellar luxation. All dogs were divided into 2 group that was group A and group B. The temperature at day 0 were 99.60 °F in group A and 99.07 °F in group B. The temperature at 4th week were 97.09 °F in group A and 99.02 °F in group B. The result …

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