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Full-Text Articles in Veterinary Medicine
The Alterations Of Fecal Microbiota In Dogs With Acute Diarrhea, Thailand, Jeerawat Soonthornsit, Natharin Ngamwongsatit, Panpanga Sangsuriya, Nlin Arya
The Alterations Of Fecal Microbiota In Dogs With Acute Diarrhea, Thailand, Jeerawat Soonthornsit, Natharin Ngamwongsatit, Panpanga Sangsuriya, Nlin Arya
The Thai Journal of Veterinary Medicine
Acute diarrhea is a common clinical sign in dogs which the pathogenesis has associated with altered gut microbiome compositions and influenced by diet, environment, and medication. However, the impact of gut microbiome on dog health has still not been well elucidated in Thailand. This study aims to investigate the alterations of the fecal microbiome in dogs with diarrhea (<3 days) compared to healthy dogs. Fresh feces were collected once from each dog, and DNA extraction was performed following the QIAamp DNA stool mini kit protocol. The 16s rRNA gene, then, amplified and analyzed by the next-generation DNA sequencing technology. The results showed a significant reduction of the evenness and Shannon index in dogs with the diarrhea group. The most dominant phylum in healthy dogs, Firmicutes, was markedly declined. Additionally, significant decreases in bacterial abundances were observed in twelve genera. To the best of our knowledge, seven of them were first described in their alterations including Clostridium sensu stricto 1, Lachnospiraceae NKA4136 group, Erysipelatoclostridium, Tyzzerella 3, Alloprevotella and Slackia. Therefore, these findings disclose the new possible biomarkers for intestinal diseases and the effect of ordinary diets on the fecal microbiome in dog health, Thailand.
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol
The Thai Journal of Veterinary Medicine
This study was conducted to investigate the dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) genotype 1 following its introduction into a herd concurrently infected with both PRRSV genotypes. The results demonstrated that 2 novel genotype 1 groups emerged, termed clusters II and III. The emergence of cluster II suggested that this cluster of genotype 1 virus potentially correlated with the outbreak. Then, cluster III was detected with the disappearance of cluster II. The evolution of the genotype 1 virus from cluster II to cluster III is likely to be associated with immune evasion induced by amino …
Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee
Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee
The Thai Journal of Veterinary Medicine
Salmonella is one of the major foodborne pathogens, often as the result of pork consumption. Currently, with the situation of antimicrobial resistance, organic farming has been suggested as an alternative for healthier options. However, there is little evidence to support this. In this study, we investigated the occurrence of Salmonella circulating in local organic pig farms in northern Thailand and typed isolated clones to better understand the population structure and transmission dynamics of the underlying Salmonella contamination. In total, 112 samples from 11 organic pig farms were processed from October to December 2018. Salmonella was detected in 9 targeted farms. …
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …
The Occurrence Of Gastrointestinal Parasitic Infections Of Goats In Ratchaburi, Thailand, Witchuta Junsiri, Pulice Tapo, Runglawan Chawengkirttikul, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Panat Anuracpreeda
The Occurrence Of Gastrointestinal Parasitic Infections Of Goats In Ratchaburi, Thailand, Witchuta Junsiri, Pulice Tapo, Runglawan Chawengkirttikul, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Panat Anuracpreeda
The Thai Journal of Veterinary Medicine
Gastrointestinal (GI) parasitic diseases play an important role in the major constraints on goat production in Thailand and worldwide. The aim of this study was to assess the occurrence of GI parasitic infections among goats in Ratchaburi province of Thailand. In this study, fecal samples (394) were collected and tested by means of the floatation, sedimentation and direct smear methods to discriminate eggs or oocyst of the parasites. The overall occurrence of GI parasitic infections was 86.54%. The respective occurrences for the raising distance, farm system, water source nearby, type of feed and aquatic plants were found to vary significantly …