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Veterinary Medicine Commons™

Open Access. Powered by Scholars. Published by Universities.®

2018

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Articles 301 - 330 of 341

Full-Text Articles in Veterinary Medicine

Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal Jan 2018

Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal

Turkish Journal of Veterinary & Animal Sciences

Cysticercosis/taeniasis is one of the neglected parasitic zoonoses in developing countries like India. A study was conducted for a period of 7 years from 2010 to 2017 during which 13,596 pig and 1238 human samples were screened to learn the exact scenario of cysticercosis/taeniasis in the different regions of Maharashtra State. Prevalence of cysticercosis/taeniasis in pigs and humans was recorded using various diagnostic methods such as meat inspection and serological methods involving ELISA. The overall prevalence of cysticercosis in pigs was found to be 0.88% by postmortem examination and 0.9% by PCR assay, whereas prevalence of taeniasis in humans was …

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Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska Jan 2018

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …

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Preference Of Side And Standing In Relationship With Milking Characteristics And Temperament Score Of Crossbred Dairy Cows In An 8 × 2 Herringbone Milking Parlour, Ahmad Fahim, Madan Lal Kamboj, Mukesh Bhakat, Tushar Kumar Mohanty, Rohit Gupta Jan 2018

Preference Of Side And Standing In Relationship With Milking Characteristics And Temperament Score Of Crossbred Dairy Cows In An 8 × 2 Herringbone Milking Parlour, Ahmad Fahim, Madan Lal Kamboj, Mukesh Bhakat, Tushar Kumar Mohanty, Rohit Gupta

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the preference of side and standing in relationship with milking characteristics of crossbred cows in a double-sided herringbone milking parlour. Seventy-two crossbred cows were milked in an 8 × 2 low-line automated herringbone milking parlour having automatic cluster removal settings for 45 milking sessions. There was considerable variation among individuals in the consistency of side choice (χ$^{2}$ = 1.414, P = 0.234). The consistency score of crossbred cows showed that many cows had a strong tendency to enter the parlour on the same side during consecutive milkings. The side preference was found …

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Ectoparasites Of Hares (Lepus Europaeus Pallas) In Konya Province, Turkey, Bi̇lal Di̇k, Uğur Uslu Jan 2018

Ectoparasites Of Hares (Lepus Europaeus Pallas) In Konya Province, Turkey, Bi̇lal Di̇k, Uğur Uslu

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to detect ectoparasites of hares (Lepus europaeus Pallas) between the years of 2003 and 2015 in Konya Province, Turkey. In this period, 75 hares shot by hunters were examined for ectoparasites macroscopically. Ectoparasites detected on the hares were collected with pliers and stored in tubes containing 70% ethanol. The ticks were identified under a stereo zoom microscope. Other parasites were cleared in 10% KOH, rinsed in distilled water, and transferred to 70%, 80%, and 99% ethanol, respectively, and mounted on slides in Canada balsam. They were identified under a binocular light microscope. The results showed that …

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Comparative Clinical And Parasitological Efficacy Of Moxidectin Pour-On, Ivermectin, And Piperazine Citrate On Toxocara Vitulorum Infection In Buffalo Calves (Bubalus Bubalis):A Randomized Clinical Trial, Mohamed Abdo Rizk, Salama A. Osman, Magdy H. Al-Gaabary, Sabry A. Elkhodery Jan 2018

Comparative Clinical And Parasitological Efficacy Of Moxidectin Pour-On, Ivermectin, And Piperazine Citrate On Toxocara Vitulorum Infection In Buffalo Calves (Bubalus Bubalis):A Randomized Clinical Trial, Mohamed Abdo Rizk, Salama A. Osman, Magdy H. Al-Gaabary, Sabry A. Elkhodery

Turkish Journal of Veterinary & Animal Sciences

Toxocara vitulorum is an important intestinal nematode that causes great economic losses in farm animals worldwide. However, there is a paucity in the development of effective and safe anthelmintic drugs against this parasite in buffalo calves. Therefore, this study aimed to evaluate the antitoxocariasis efficacy of moxidectin, ivermectin, and piperazine citrate in buffalo calves. To achieve this purpose, 130 buffalo calves were randomly selected from Kafr El-Sheikh governorate, Egypt, and were examined for the presence of Toxocara vitulorum. Thirty calves were identified to be infected. Diseased calves were allocated randomly into 3 groups (10 each). The first group received moxidectin …

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Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ Jan 2018

Seroepidemiology And Molecular Investigation Of Pestiviruses Among Sheep And Goats In Northwest Anatolia, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Pestiviruses, which are distributed worldwide, may cause losses in sheep and goat populations. The current seroprevalence and circulation of pestiviruses in small ruminants of the South Marmara Region of Turkey were investigated. A total of 607 blood samples were collected from 22 different flocks in 4 different provinces, and 24 necropsy materials were investigated. Pestivirus seroprevalence in sheep and goats was determined by virus neutralization test against the NADL strain of bovine viral diarrhea virus. The mean antibody titer was 3.5, while the overall seropositivity rate was 32.78% (199/607). Seronegativity was markedly more prevalent in goats. The mean detected antibody …

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Comparison Of The In Vitro Anticancer Effect Of Habanero Pepper Extract Containing Capsaicin With That Of Pure Capsaicin In Selected Dog Neoplastic Cell Lines, Lukasz Adaszek, Olga Slabczy?Ska, Pawel Lyp, Dagmara Gadomska, Jerzy Zietek, Dorota Rozanska, Maciej Orzelski, Anna Smiech, Marta Staniec, Dorota Krasucka, Stanislaw Winiarczyk Jan 2018

Comparison Of The In Vitro Anticancer Effect Of Habanero Pepper Extract Containing Capsaicin With That Of Pure Capsaicin In Selected Dog Neoplastic Cell Lines, Lukasz Adaszek, Olga Slabczy?Ska, Pawel Lyp, Dagmara Gadomska, Jerzy Zietek, Dorota Rozanska, Maciej Orzelski, Anna Smiech, Marta Staniec, Dorota Krasucka, Stanislaw Winiarczyk

Turkish Journal of Veterinary & Animal Sciences

The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer lines, DAN (fibroblasts isolated from osteosarcoma) and D-17 (epithelium cells of osteosarcoma obtained from pulmonary metastatic tumors), under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation (37 °C, 5% CO2), the fluid above the culture was removed, and diluted habanero pepper extract was added. The concentrations of capsaicin in …

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The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis Jan 2018

The Effects Of Dietary Biotechnological Products Of Saccharomices Cerevisiae On Growth Performance, Health Status, And Meat Composition In Broiler Chickens, Ionel Toader, Mihai Iacob Bentea, Ilie Cornoiu, Otilia Bobis

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to investigate the effects of three biotechnological products of Saccharomyces cerevisiae (organic selenium, concentrated mannan of S. cerevisiae, and living cells of the same yeast) used in broiler diet on growth performance, health status, and meat quality and composition. One hundred day-old broiler chickens were divided into four groups (control and three experimental groups using the above-mentioned diet supplements) and fed basic feed mixture (developed by the Nutrition Department at the University of Agricultural Sciences and Veterinary Medicine in Cluj-Napoca, Romania) for 42 days. After this period, from each group, five birds were sacrificed and …

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Effects Of Organic And Inorganic Nitrogen Sources On In Vitro Degradability Of Citrus Byproduct And Milk Thistle, Asma Aggoun, Mohamed Laid Haddi, Si-Yong Yang, Jong-Su Eun, Andrea Squartini Jan 2018

Effects Of Organic And Inorganic Nitrogen Sources On In Vitro Degradability Of Citrus Byproduct And Milk Thistle, Asma Aggoun, Mohamed Laid Haddi, Si-Yong Yang, Jong-Su Eun, Andrea Squartini

Turkish Journal of Veterinary & Animal Sciences

In order to evaluate rumen microbial activity, the effects of two nitrogen sources (organic and inorganic) were analyzed in vitro by inoculating dairy cow rumen fluid and using citrus byproduct (CBP) and milk thistle (Silybum marianum, MT) as energy source substrates. The experimental design was 2 × 3 where we used three different nitrogen sources (N1: no nitrogen added, N: ammonium bicarbonate, N3: sodium glutamate) with a basic concentration of nitrogen (100 mg/1 L). Culture pH, dry matter degradability %, ammonia-N (NH$_{3}$-N), total volatile fatty acids (TVFAs), individual volatile fatty acid proportions, and acetate propionate ratio (A:P) were measured after …

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Comparison Between The Anaesthetic Effects Of Xylazine?Ketamine And Diazepam?Ketamine: Physiological And Blood Parameters In Young Hamadryas Baboons (Papio Hamadryas), Fahd Alsobayil, Madeh Sadan, Abd Al-Azeez Meshaal Al-Shoshan Jan 2018

Comparison Between The Anaesthetic Effects Of Xylazine?Ketamine And Diazepam?Ketamine: Physiological And Blood Parameters In Young Hamadryas Baboons (Papio Hamadryas), Fahd Alsobayil, Madeh Sadan, Abd Al-Azeez Meshaal Al-Shoshan

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the anaesthetic effects on young hamadryas baboons (Papio hamadryas) of xylazine-ketamine (XK) compared to diazepam-ketamine (DK). Six healthy young male hamadryas baboons were first premedicated with xylazine HCl (0.5 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). After a 10-day washout period, the hamadryas baboons were premedicated with diazepam (1 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). The onset, duration, and the depth of anaesthesia were determined by recording palpebral, corneal, and jaw reflexes. The results showed a significant decrease in heart rate …

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First Evidence Of Histological And Histochemical Intraspecific Differences In Salivary Glands In Brown Norway And Albino Wistar Rats, Flavia Ruxanda, Cristian Ratiu, Bianca Matosz, Radu Constantinescu, Viorel Miclaus Jan 2018

First Evidence Of Histological And Histochemical Intraspecific Differences In Salivary Glands In Brown Norway And Albino Wistar Rats, Flavia Ruxanda, Cristian Ratiu, Bianca Matosz, Radu Constantinescu, Viorel Miclaus

Turkish Journal of Veterinary & Animal Sciences

The size of salivary glands, acinar structure, and secretion particularities differ from one species to another and are influenced by diet and environment. In order to investigate whether there are histological or histochemical differences in the salivary glands of animals from the same species, bred in identical conditions of environment and diet, we assessed two laboratory rat strains. The salivary glands from two rat strains were processed for histological and histochemical investigations. Histologically, we noticed differences in the parotid gland: seromucous acini were present in Brown Norway rat and typical serous ones in Wistar rat. Histochemically, we observed that cells …

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Evaluation Of Biological Response Modification And Immunotherapeutic Activities Of Barley-Derived Arabinoxylans Against Coccidiosis In Commercial Broilers, Masood Akhtar, Muteen Humayun, Zafar Iqbal, Mian Muhammad Awais, Muhammad Irfan Anwar Jan 2018

Evaluation Of Biological Response Modification And Immunotherapeutic Activities Of Barley-Derived Arabinoxylans Against Coccidiosis In Commercial Broilers, Masood Akhtar, Muteen Humayun, Zafar Iqbal, Mian Muhammad Awais, Muhammad Irfan Anwar

Turkish Journal of Veterinary & Animal Sciences

Plant-derived polysaccharides have been reported for diverse biological response modification (BRM) and immunotherapeutic (IT) activities in animals by activating their defense systems to perform better immunogenically against invading pathogens. Keeping this in view, this study was conducted to assess the BRM and IT activities of barley (Hordeum vulgare L.)-derived arabinoxylans (AXs) against coccidiosis in broilers. Significantly enhanced (P < 0.05) in vivo and in vitro lymphoproliferative responses to T-cell mitogens were revealed in broilers administered barley AXs when compared to the control group. Humoral immune response in terms of antibody titers against sheep erythrocytes was also found higher (P < 0.05) in AX-administered chickens. Furthermore, AX-administered chickens also showed higher weekly weight gains and improved feed conversion ratios as compared to controls. Results of the challenge experiment showed that the percentage of protection and daily weight gains were statistically higher (P < 0.05) whereas mean oocysts per gram of droppings and lesion scores were significantly lower (P < 0.05) in the chickens administered barley AXs as compared to controls. In conclusion, barley-derived AXs led to better growth performance and elicited humoral and cellular immune responses in commercial broilers that persisted against coccidial infection in broiler chickens.

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Virulence-Associated Genes And Molecular Typing Of Streptococcus Uberisassociated With Bovine Mastitis In Northern Thailand, Sukolrat Boonyayatra, Prasit Tharavichitkul, Stephen Oliver Jan 2018

Virulence-Associated Genes And Molecular Typing Of Streptococcus Uberisassociated With Bovine Mastitis In Northern Thailand, Sukolrat Boonyayatra, Prasit Tharavichitkul, Stephen Oliver

Turkish Journal of Veterinary & Animal Sciences

Streptococcus uberis is a major bovine mastitis pathogen. Several studies have revealed a persistence of specific strains of S. uberis with enhanced virulence or transmissibility. We aimed to investigate the presence of S. uberis virulence-associated genes including plasminogen activator (pauA), glyceraldehyde-3-phosphate dehydrogenase (gapC), oligopeptide permease (oppF), metal transporter uberis A (mtuA), hyaluronic acid capsules (hasA, hasB, hasC), lactoferrin binding protein (lbp), adhesion protein (sua), and CAMP factor (cfu) as well as the genetic profiles using pulsed-field gel electrophoresis (PFGE) among isolates from cases of clinical mastitis, subclinical mastitis, and intramammary infection with low somatic cell count in dairy cows. A …

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Hematological And Clinical Studies In West African Dwarf Sheepexperimentally Infected With Trypanosoma Brucei And Treated Withdiminazene Aceturate, Levamisole, And Vitamin A, Clara Akpan, Nwakaego Nweze, Cornelius Chukwu Jan 2018

Hematological And Clinical Studies In West African Dwarf Sheepexperimentally Infected With Trypanosoma Brucei And Treated Withdiminazene Aceturate, Levamisole, And Vitamin A, Clara Akpan, Nwakaego Nweze, Cornelius Chukwu

Turkish Journal of Veterinary & Animal Sciences

The effects of diminazene aceturate (DA), levamisole, and/or vitamin A on hematological and clinical parameters of West African dwarf sheep experimentally infected with Trypanosoma brucei were studied. Twenty-four adult sheep were randomly assigned to 6 groups of 4 animals. They were infected with 1 × 10$^{6}$ trypanosomes intravenously (groups 2?6) or were uninfected (group 1). Treatment was administered 2 weeks post infection (PI) in all treated groups, except group 5, which was treated 3 weeks PI. Group 2 (positive control) received 7 mg/kg DA. Group 3 received 7 mg DA and 5 mg levamisole per kilogram of body weight. Group …

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Effect Of Epidural Dexmedetomidine With Or Without Local Anesthetics On Painscore And Serum Il-6 Levels In Dogs Undergoing Elective Ovariohysterectomy, Mudasir Ahmad Shah, Prakash Kinjavdekar, Amarpal -, Deepti Sharma, Abhijit Motiram Pawde, Abu Rafee Malik, Abas Rashid Bhat Jan 2018

Effect Of Epidural Dexmedetomidine With Or Without Local Anesthetics On Painscore And Serum Il-6 Levels In Dogs Undergoing Elective Ovariohysterectomy, Mudasir Ahmad Shah, Prakash Kinjavdekar, Amarpal -, Deepti Sharma, Abhijit Motiram Pawde, Abu Rafee Malik, Abas Rashid Bhat

Turkish Journal of Veterinary & Animal Sciences

To evaluate the postoperative analgesic effects of epidural dexmedetomidine with or without local anesthetics, prospective randomized double-blinded clinical trials were performed with 24 healthy client-owned mixed-breed adult dogs with an average weight of 18.1 ± 2.4 kg allotted equally (n = 6) to four groups: A, B, C, and D. All animals were premedicated intramuscularly with atropine (0.04 mg/kg) and after 5 min by intravenous midazolam (0.7 mg/kg). After 10 min of premedication, in group A dexmedetomidine (7 µg/kg) and in groups B, C, and D, in addition to dexmedetomidine (7 µg/kg), lidocaine (4.4 mg/kg), bupivacaine (2 mg/kg), and ropivacaine …

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The Application Of Edta-Tris And Chlorhexidine In The Treatment Of Endometritis As A Replacement For Antibiotic Therapy In Cows, Igor Stojanov, Aleksandar Milovanovic, Dragana Ruzic-Muslic, Radomir Ratajac, Milica Zivkov Balos, Nevena Maksimovic, Jelena Apic Jan 2018

The Application Of Edta-Tris And Chlorhexidine In The Treatment Of Endometritis As A Replacement For Antibiotic Therapy In Cows, Igor Stojanov, Aleksandar Milovanovic, Dragana Ruzic-Muslic, Radomir Ratajac, Milica Zivkov Balos, Nevena Maksimovic, Jelena Apic

Turkish Journal of Veterinary & Animal Sciences

The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. …

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Identification Of Sarcocystis Spp. By Polymerase Chain Reaction And Microscopic Examination In Various Beef Products (Minced Meat, Meatballs, Fermented Sausage), Ufuk Kamber, Mükremi̇n Özkan Arslan, Güven Gülbaz, Gencay Taşkin Taşçi, Ati̇la Akça Jan 2018

Identification Of Sarcocystis Spp. By Polymerase Chain Reaction And Microscopic Examination In Various Beef Products (Minced Meat, Meatballs, Fermented Sausage), Ufuk Kamber, Mükremi̇n Özkan Arslan, Güven Gülbaz, Gencay Taşkin Taşçi, Ati̇la Akça

Turkish Journal of Veterinary & Animal Sciences

Sarcocystis species are the most commonly encountered parasitic organisms in animals. Sarcocystis hominis (Sarcocystis bovihominis), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been established that, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes food poisoning. Therefore, this study was undertaken in order to determine the extent to which Sarcocystis species are present in minced meat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, were collected in …

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Gene Expression Levels In Some Candidate Genes For Mastitis Resistance, Milk Yield, And Milk Quality Of Goats Reared Under Different Feeding Systems, Akin Yakan, Hüseyi̇n Özkan, Aysel Eraslan Şakar, Necmetti̇n Ünal, Ceyhan Özbeyaz Jan 2018

Gene Expression Levels In Some Candidate Genes For Mastitis Resistance, Milk Yield, And Milk Quality Of Goats Reared Under Different Feeding Systems, Akin Yakan, Hüseyi̇n Özkan, Aysel Eraslan Şakar, Necmetti̇n Ünal, Ceyhan Özbeyaz

Turkish Journal of Veterinary & Animal Sciences

This study aimed to identify the expression levels of some candidate genes for mastitis resistance (LTF and BRCA1), milk yield (POU1F1 and IGF-1), and milk quality (PPARγ and CSN2) in different feeding systems (pen- and pasture-based) used for Damascus goats. Milk samples were taken from all goats in both pen and pasture groups at 4 times, at the 1st, 3rd, 5th, and 7th months of the study. It was found that, compared to the pen group, the LTF and CSN2 genes in the pasture group had significant levels of upregulation in the 7th month of lactation (3.538- and 5.436-fold, respectively), …

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Multi-Drug Resistant Escherichia Coli In Diarrhoeagenic Foals: Pulsotyping, Phylotyping, Serotyping, Antibiotic Resistance And Virulence Profiling, C.A. Kennedy, Ciara Walsh, M. Karczmarczyk, S. O'Brien, N. Akasheh, M. Quirke, S. Farrell-Ward, T. Buckley, U. Fogherty, K. Kavanagh, C.T. Parker, T. Sweeney, S. Fanning Jan 2018

Multi-Drug Resistant Escherichia Coli In Diarrhoeagenic Foals: Pulsotyping, Phylotyping, Serotyping, Antibiotic Resistance And Virulence Profiling, C.A. Kennedy, Ciara Walsh, M. Karczmarczyk, S. O'Brien, N. Akasheh, M. Quirke, S. Farrell-Ward, T. Buckley, U. Fogherty, K. Kavanagh, C.T. Parker, T. Sweeney, S. Fanning

Articles

Extraintestinal pathogenic E. coli (ExPEC) possess the ability to cause extraintestinal infections such as urinary tract infections, neonatal meningitis and sepsis. While information is readily available describing pathogenic E. coli populations in food-producing animals, studies in companion/sports animals such as horses are limited. In addition, many antimicrobial agents used in the treatment of equine infections are also utilised in human medicine, potentially contributing to the spread of antibiotic resistance determinants among pathogenic strains. The aim of this study was to phenotypically and genotypically characterise the multidrug resistance and virulence associated with 83 equine E. coli isolates recovered from foals with …

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Physiological And Immunological Responses Of Porcine Primary Endometrial Cells To Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Infection, Muttarin Lothong Jan 2018

Physiological And Immunological Responses Of Porcine Primary Endometrial Cells To Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Infection, Muttarin Lothong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is highly limited to only cell subsets that express PRRSV receptors. Reproductive organ revealing the typical signs of PRRSV infection may be the critical site of problem syndromes. Persistent PRRSV producing re-infection via horizontal or vertical transmission could not be eradicated from herds. This research examined the possibility of porcine endometrium to be a PRRSV permissive cell and serves as the primary cause of the persistent PRRSV. Cellular and immunological in response to PRRSV relevant to viral replication, shedding and re-circulation was assessed. The different outcomes between the different genotypes (type I vs. …

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Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum Jan 2018

Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main overall goal of this study is to investigate the application of modified chitosan as a potential vector for apoptotic gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells (i.e. testicular cells and mammary cancer cells). This study reported Gonadotropin Releasing Hormone-modified Chitosan (GnRH-CS) nanoparticle as a promising vector for targeted gene delivery, and a GnRH peptide was used for active targeting of a transgene in GnRHR expressing cells. The physiological characterizations of the prepared nanoparticle were investigated by proton nuclear magnetic resonance spectroscopy (1H NMR), Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Nanosizer. The prepared GnRH-CS was …

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Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti Jan 2018

Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed …

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The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya Jan 2018

The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya

Chulalongkorn University Theses and Dissertations (Chula ETD)

Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG …

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Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka Jan 2018

Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka

Chulalongkorn University Theses and Dissertations (Chula ETD)

Synbiotics, a synergistic combination of probiotics and prebiotics, are now highlighted as one of the most practical nutritional supplements in red tilapia farms. The aim of the present study was to assess the effect of the dietary inclusion of Jerusalem artichoke, Lactobacillus rhamnosus, or their combination, on serum biochemical, growth performance, intestinal morphology, immune parameters, intestinal bacterial count and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.). One hundred and eighty male red tilapias (average body weight of 14.05±0.42 g) were distributed into six 1000-liter tanks containing water under continuous aeration and an average temperature of 25–28°C. Tilapias …

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The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad Jan 2018

The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea is caused by porcine epidemic diarrhea virus (PEDV), which causes swine watery diarrhea and higher mortality rates in piglets less than one week of age. Outbreaks of PED affect to economic loss, especially in a swine industrial country. Many strategies were used for preventing the outbreak and infection of PEDV, especially maternal antibody stimulation in pigs and then transfer to piglet through colostrum or milk. Thus, the investigation of antibody level for the situation on the farm is required. The method which convenient and rapid than viral neutralization (VN) test is an enzyme-linked immunosorbent assay (ELISA). The …

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Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij Jan 2018

Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brugia pahangi is a major causative agent of animal lymphatic filariasis, one of the neclected tropical disease. Recent studies have also indicated that B. pahangi can cause clinical infection in human, with clinical appearances which are consistent with human lymphatic filariasis. Nowadays, the recognition of Wolbachia as new alternative drug choice for treatment and control of lymphatic filariasis is the most important in anti-filarial chemotherapy. Since, Wolbachia play important roles, including the development, viability, fertility and fecundity of filarial parasites. Depletion of Wolbachia results in sterilization, stunting and death of filarial worms. In this study, we investigated the effects of …

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Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen Jan 2018

Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Retinal (retinaldehyde) is one of natural vitamin A metabolites. It is widely used in clinical dermatology such as acne, acne scar, photoaging, wrinkles, psoriasis, skin neoplasia and seborrheic dermatitis. Retinal is a pivotal key to regulate keratinocyte proliferation and differentiation in the epidermis resulting in epidermal thickening and inhibit collagen destruction in the dermis. However, topical application of retinal is still irritative to the skin and chemically and photochemically unstable. Topically applied retinal can induce a retinoid dermatitis. This inflammation is induced by an overload of non-physiological amounts of exogenous retinoic acid in the skin. The adverse effects and physicochemical …

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Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn Jan 2018

Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and …

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Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain Jan 2018

Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain

Chulalongkorn University Theses and Dissertations (Chula ETD)

Live bird markets play a significant role in highly pathogenic avian influenza (HPAI) virus spread and transmission. In Myanmar, the first HPAI-H5N1 outbreak was reported in 2006. Since 2014, routine influenza A virus surveillance in LBMs have been conducted in Myanmar by the Livestock Breeding and Veterinary Department in cooperation with FAO. However, there is limited information on virus characterization and evolution of influenza viruses in Myanmar. The objectives of this thesis were to detect influenza virus subtypes circulating in different poultry species from Mingalar-Taung-Nyunt live bird market (MTN-LBM) and to determine genetic characteristics and genetic diversity of influenza A …

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Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri Jan 2018

Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter has been known as an important cause of gastroenteritis in humans. The primary source is raw or undercooked poultry meat. Previous study showed that Campylobacter isolated from slaughterhouses were associated with Campylobacter isolated from broiler farms. Thus, detection of Campylobacter flock status and sorting flocks prior to slaughtering processes may help prevent Campylobacter contamination between Campylobacter-positive and negative flocks during slaughtering processes. Unfortunately, appropriate sample type and isolation method have not been clearly specified for Campylobacter detection at the farm level. Because Campylobacter are fastidious bacteria, improper detection method can attribute to false negative results. Therefore, the objectives of …

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