Veterinary Medicine Commons^™

Tellurite Resistance In Shiga Toxin-Producing Escherichia Coli , Gentry L. Lewis, Quentin R. Jorgensen, John Dustin Loy, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Potassium tellurite ( K₂TeO₃) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K₂TeO₃ resistance in STEC is attributable to the ter gene cluster with terD an indicator of the cluster’s presence. Polymerase chain reactions for terD and K₂TeO₃ minimum inhibitory concentration (MIC) determinations in broth cultures were conducted on 70 STEC and 40 non-STEC control organisms. Sixty-six STEC strains (94.3%) were terD+ compared to 28 control organisms (70.0%; P < 0.001). The prevalence of terD in …

Epitope Mapping Of Serca2a Identifies An Antigenic Determinant That Induces Mainly Atrial Myocarditis In A/J Mice, Bharathi Krishnan, Chandirasegara Massilamany, Rakesh H. Basavalingappa, Arunakumar Gangaplara, Rajkumar Rajasekaran, Muhammad Z. Afzal, Vahid Khalilzad-Sharghi, You Zhou, Jean-Jack Riethoven, Shyam S. Nandi, Paras K. Mishra, Raymond A. Sobel, Jennifer L. Strande, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Sarcoplasmic/endoplasmic reticulum Ca²⁺ adenosine triphosphatase (SERCA)2a, a critical regulator of calcium homeostasis, is known to be decreased in heart failure. Patients with myocarditis or dilated cardiomyopathy develop autoantibodies to SERCA2a suggesting that they may have pathogenetic significance. In this report, we describe epitope mapping analysis of SERCA2a in A/J mice that leads us to make five observations: 1) SERCA2a contains multiple T cell epitopes that induce varying degrees of myocarditis. One epitope, SERCA2a 971–990, induces widespread atrial inflammation without affecting noncardiac tissues; the cardiac abnormalities could be noninvasively captured by echocardiography, electrocardiography, and magnetic resonance microscopy imaging. 2) SERCA2a …

Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt

School of Veterinary and Biomedical Sciences: Faculty Publications

Hydrated lime manure treatment was evaluated to determine porcine epidemic diarrhea virus (PEDV) susceptibility to alkaline stabilization. At pH 10, PEDV decreased (quantitative polymerase chain reaction) and lost infectivity (swine bioassay). Although ammonium decreased above pH 9 (up to 25%), alkaline stabilization managed to control potential infection from manure sources.

Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry Identification Of Moraxella Bovoculi And Moraxella Bovis Isolates From Cattle, Kara Robbins, Aaron M. Dickey, Michael L. Clawson, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious bovine keratoconjunctivitis (IBK) is an economically significant disease caused by Moraxella bovis. Moraxella bovoculi, although not reported to cause IBK, has been isolated from the eyes of cattle diagnosed with IBK. Identification of M. bovis and M. bovoculi can be performed using biochemical or DNA-based approaches, both of which may be time consuming and inconsistent between laboratories. We conducted a comparative evaluation of M. bovoculi and M. bovis identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a database provided by Bruker Daltonics (termed the BDAL database), the BDAL database supplemented with spectra generated in …

Development Of A Multiplex Real-Time Pcr Assay Using Two Thermocycling Platforms For Detection Of Major Bacterial Pathogens Associated With Bovine Respiratory Disease Complex From Clinical Samples, John Dustin Loy, Laura Leger, Aspen M. Workman, Michael L. Clawson, Ece Bulut, Bing Wang

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine respiratory disease complex (BRDC) is one of the most significant diseases of cattle. Bacterial pathogens involved in BRDC include Mannheimia haemolytica, Mycoplasma bovis, Histophilus somni, and Pasteurella multocida. We developed and evaluated a multiplexed real-time hydrolysis probe (rtPCR) assay using block-based Peltier and rotary-based thermocycling on lung tissue, nasal swabs, and deep nasopharyngeal swabs. The rtPCR results were compared to culture or a gel-based M. bovis PCR using statistical analysis to determine optimum quantification cycle (Cq) cutoffs to maximize agreement. The limits of detection were 1.2–12 CFU/reaction for each pathogen. M. haemolytica was the most prevalent organism detected …

Coordinated Regulation Of Transcription By Ccpa And The Staphylococcus Aureus Twocomponent System Hptrs, Joseph M. Reed, Sean Olson, Danielle F. Brees, Caitlin E. Griffin, Ryan A. Grove, Paul J. Davis, Stephen D. Kachman, Jiri Adamec, Greg A. Somerville

School of Veterinary and Biomedical Sciences: Faculty Publications

The success of Staphylococcus aureus as a pathogen is due in part to its ability to adapt to changing environmental conditions using signal transduction pathways, such as metaboliteresponsive regulators and two-component systems. S. aureus has a two-component system encoded by the gene pair sav0224 (hptS) and sav0223 (hptR) that regulate the hexose phosphate transport (uhpT) system in response to extracellular glucose-6-phosphate. Glycolytic intermediates such as glucose-6-phosphate are important carbon sources that also modulate the activity of the global metabolite-responsive transcriptional regulator CcpA. Because uhpT has a putative CcpA binding site in its promoter and …

Novel Docosahexaenoic Acid Ester Of Phloridzin Inhibits Proliferation And Triggers Apoptosis In An In Vitro Model Of Skin Cancer, Theodora Mantso, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, H. P. Vasantha Rupasinghe, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Skin cancer is among the most common cancer types accompanied by rapidly increasing incidence rates, thus making the development of more efficient therapeutic approaches a necessity. Recent studies have revealed the potential role of decosahexaenoic acid ester of phloridzin (PZDHA) in suppressing proliferation of liver, breast, and blood cancer cell lines. In the present study, we investigated the cytotoxic potential of PZDHA in an in vitro model of skin cancer consisting of melanoma (A375), epidermoid carcinoma (A431), and non-tumorigenic (HaCaT) cell lines. Decosahexaenoic acid ester of phloridzin led to increased cytotoxicity in all cell lines as revealed by cell viability …

Whole Genome Sequencing Of Moraxella Bovoculi Reveals High Genetic Diversity And Evidence For Interspecies Recombination At Multiple Loci, Aaron M. Dickey, Gennie Schuller, John D. Loy, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is frequently cultured from the ocular secretions and conjunctiva of cattle with Infectious Bovine Keratoconjunctivitis (IBK). Previous work has shown that single nucleotide polymorphism (SNP) diversity in this species is quite high with 81,284 SNPs identified in eight genomes representing two distinct genotypes isolated from IBK affected eyes (genotype 1) and the nasopharynx of cattle without clinical IBK signs (genotype 2), respectively. The goals of this study were to identify SNPs from a collection of geographically diverse and epidemiologically unlinked M. bovoculi strains from the eyes of IBK positive cattle (n = 183) and another from the eyes …

Differential Modulation Of Human Gabac‑Ρ1 Receptor By Sulfur‑Containing Compounds Structurally Related To Taurine, Lenin David Ochoa‑De La Paz, Martin González‑Andrade, Herminia Pasantes‑Morales, Rodrigo Franco, Rubén Zamora‑Alvarado, Edgar Zenteno, Hugo Quiroz‑Mercado, Roberto Gonzales‑Salinas, Rosario Gulias‑Cañizo

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: The amino acid taurine (2-Aminoethanesulfonic acid) modulates inhibitory neurotransmitter receptors. This study aimed to determine if the dual action of taurine on GABAC- ρ1R relates to its structure. To address this, we tested the ability of the structurally related compounds homotaurine, hypotaurine, and isethionic acid to modulate GABAC- ρ1R.

Results: In Xenopus laevis oocytes, hypotaurine and homotaurine partially activate heterologously expressed GABAC- ρ1R, showing an increment in its deactivation time with no changes in channel permeability, whereas isethionic acid showed no effect. Competitive assays suggest that hypotaurine and homotaurine compete for the GABA-binding site. In addition, their effects were …

Using Art And Story To Explore How Primary School Students In Rural Tanzania Understand Planetary Health: A Qualitative Analysis, Elizabeth Vanwormer, Jesca Mlawa, Elizabeth Komba, Christopher Gustafson, Hilda Mrema, Jenny M. Dauer

School of Veterinary and Biomedical Sciences: Faculty Publications

Background The global planetary health community increasingly recognises the need to prepare students to investigate and address connections between environmental change and human health. As we strive to support education on planetary health themes for students of all ages, understanding students’ concepts of linkages between the health of people and animals, and their shared environments might advance educational approaches. Children living in villages bordering Ruaha National Park in Iringa Region, Tanzania, have direct experience of these connections as they share a water-stressed but biodiverse environment with domestic animals and wildlife. Livelihoods in these villages depend predominantly on crop and livestock …

The Unspoken Psychological Issues With Lesson Horses And How We Need To Address Them, Casey Clark

Murray State Theses and Dissertations

Every day all over the world horses provide much needed therapy and treatment to people in need. They provide physical, emotional, and mental training and healing. Humans depend on them for so much and we have a moral and ethical obligation to be good stewards of our equine companions. This close proximity can take a toll on horses physically and psychologically even in the realm of experienced equine professionals. So many times though it is the small lesson facility that sees hundreds of people each year and makes a profound difference in people’s lives. This can be at a 4-H …

Follicular Responsiveness To Equine Chorionic Gonadotropin In Cat Species: Study In In Vitro Culture, Ajjima Chansaenroj

Chulalongkorn University Theses and Dissertations (Chula ETD)

Equine chorionic gonadotropin (eCG) has been commonly used to induce estrus in several felid species. However, the mechanisms by which this gonadotropin regulates cat folliculogenesis are still unclear. In this present study, we investigated 1) the in vitro responsiveness of cat ovarian follicles at different follicle developmental stages to various eCG concentration; 2) tiger antral follicle responsiveness to eCG; 3) the influence of eCG combination with insulin-like growth factor I (IGF-I) and/or stem cell factor (SCF) on cat ovarian follicles at different developmental stages. Study 1, the isolated follicles from the ovaries of 22 cats were classified into three developmental …

The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea is caused by porcine epidemic diarrhea virus (PEDV), which causes swine watery diarrhea and higher mortality rates in piglets less than one week of age. Outbreaks of PED affect to economic loss, especially in a swine industrial country. Many strategies were used for preventing the outbreak and infection of PEDV, especially maternal antibody stimulation in pigs and then transfer to piglet through colostrum or milk. Thus, the investigation of antibody level for the situation on the farm is required. The method which convenient and rapid than viral neutralization (VN) test is an enzyme-linked immunosorbent assay (ELISA). The …

Identification Of Sarcocystis Spp. By Polymerase Chain Reaction And Microscopic Examination In Various Beef Products (Minced Meat, Meatballs, Fermented Sausage), Ufuk Kamber, Mükremi̇n Özkan Arslan, Güven Gülbaz, Gencay Taşkin Taşçi, Ati̇la Akça

Turkish Journal of Veterinary & Animal Sciences

Sarcocystis species are the most commonly encountered parasitic organisms in animals. Sarcocystis hominis (Sarcocystis bovihominis), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been established that, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes food poisoning. Therefore, this study was undertaken in order to determine the extent to which Sarcocystis species are present in minced meat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, were collected in …

Effect Of Epidural Dexmedetomidine With Or Without Local Anesthetics On Painscore And Serum Il-6 Levels In Dogs Undergoing Elective Ovariohysterectomy, Mudasir Ahmad Shah, Prakash Kinjavdekar, Amarpal -, Deepti Sharma, Abhijit Motiram Pawde, Abu Rafee Malik, Abas Rashid Bhat

Turkish Journal of Veterinary & Animal Sciences

To evaluate the postoperative analgesic effects of epidural dexmedetomidine with or without local anesthetics, prospective randomized double-blinded clinical trials were performed with 24 healthy client-owned mixed-breed adult dogs with an average weight of 18.1 ± 2.4 kg allotted equally (n = 6) to four groups: A, B, C, and D. All animals were premedicated intramuscularly with atropine (0.04 mg/kg) and after 5 min by intravenous midazolam (0.7 mg/kg). After 10 min of premedication, in group A dexmedetomidine (7 µg/kg) and in groups B, C, and D, in addition to dexmedetomidine (7 µg/kg), lidocaine (4.4 mg/kg), bupivacaine (2 mg/kg), and ropivacaine …

Comparative Clinical And Parasitological Efficacy Of Moxidectin Pour-On, Ivermectin, And Piperazine Citrate On Toxocara Vitulorum Infection In Buffalo Calves (Bubalus Bubalis):A Randomized Clinical Trial, Mohamed Abdo Rizk, Salama A. Osman, Magdy H. Al-Gaabary, Sabry A. Elkhodery

Turkish Journal of Veterinary & Animal Sciences

Toxocara vitulorum is an important intestinal nematode that causes great economic losses in farm animals worldwide. However, there is a paucity in the development of effective and safe anthelmintic drugs against this parasite in buffalo calves. Therefore, this study aimed to evaluate the antitoxocariasis efficacy of moxidectin, ivermectin, and piperazine citrate in buffalo calves. To achieve this purpose, 130 buffalo calves were randomly selected from Kafr El-Sheikh governorate, Egypt, and were examined for the presence of Toxocara vitulorum. Thirty calves were identified to be infected. Diseased calves were allocated randomly into 3 groups (10 each). The first group received moxidectin …

Comparison Between The Anaesthetic Effects Of Xylazine?Ketamine And Diazepam?Ketamine: Physiological And Blood Parameters In Young Hamadryas Baboons (Papio Hamadryas), Fahd Alsobayil, Madeh Sadan, Abd Al-Azeez Meshaal Al-Shoshan

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the anaesthetic effects on young hamadryas baboons (Papio hamadryas) of xylazine-ketamine (XK) compared to diazepam-ketamine (DK). Six healthy young male hamadryas baboons were first premedicated with xylazine HCl (0.5 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). After a 10-day washout period, the hamadryas baboons were premedicated with diazepam (1 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). The onset, duration, and the depth of anaesthesia were determined by recording palpebral, corneal, and jaw reflexes. The results showed a significant decrease in heart rate …

Effect Of Ractopamine Hydrochloride On Growth Promotion In Guinea Pigs (Cavia Porcellus), Irene Citlalli Zuñiga Galindo, Jaime Alonso Navarro Hernandez, Shalaiko Christian Carlin Valderrabano, Julio Cesar Sanchez Lemus, Ivan Juarez Rodriguez, Dinorah Vargas Estrada

Turkish Journal of Veterinary & Animal Sciences

Ractopamine hydrochloride (R) is commonly used in the livestock industry, mainly in pork and beef, to improve feed efficiency and productivity. In the present study, the use of R at different doses (20 ppm, 30 ppm, or 40 ppm) on growth performance and carcass characteristics of guinea pigs (male and female) was explored with a view to achieving greater use of this species for human consumption. Thirty-two clinically healthy guinea pigs at an average age of 5 weeks were used. The daily gain, feed over gain ratio, Kleiber ratio, carcass yield, and muscle thickness of the thigh in millimeters were …

Survey Of Dirofilaria Immitis In Dogs From Sivas Province In Thecentral Anatolia Region Of Turkey, Ahmet Duran Ataş, Kürşat Altay, Ahmet Ali̇m, Erkan Özkan

Turkish Journal of Veterinary & Animal Sciences

In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All …

Congenital Tuberculosis In A 25-Day-Old Female Calf, Maria Del Carmen Espejel Del Moral, Juan Carlos Del Rio, Alfredo Medrano, Marco Antonio Santillan, Jose Francisco Morales

Turkish Journal of Veterinary & Animal Sciences

A female calf, housed in a dairy intensive-system farm, suddenly died 25 days after birth. Routine necropsy revealed multiple variably sized white to yellowish nodules in the lungs. Histopathological diagnosis showed severe granulomatous pneumonia with acid-fast bacilli; Mycobacterium bovis was confirmed by polymerase chain reaction and culture isolation. Congenital tuberculosis was diagnosed based on these findings. The calf probably acquired the infection from her mother through aspiration of the amniotic fluid. The aim here is to describe the morphological lesions and complementary confirmation of congenital tuberculosis in a young female calf.

Application Of Reproductive Biotechnologies For Sustainableproduction Of Livestock In Turkey, Abdullah Kaya, Erdoğan Güneş, Erdoğan Memi̇li̇

Turkish Journal of Veterinary & Animal Sciences

Reproductive biotechnology encompasses powerful tools to enhance the efficiency and profitability of livestock reproduction, production, and product quality. These technologies include genomic selection, evaluation of semen (from bulls, bucks, and rams) using advanced cell and molecular technologies, semen cryopreservation and artificial insemination, estrus synchronization, superovulation of the females (cows, dams, and ewes), ovum pick-up, in vitro fertilization and embryo culture, embryo transfer, and pregnancy detection. The extent of applications of these modern technologies and their economic impacts for livestock production in Turkey are elusive. As a result, there is an urgent need for sound economic analyses, research and education, training …

Immunogenicity Of Transmembrane Deleted-G Protein Of Bovine Ephemeral Fever Virus (Befv) Expressed In Insect Cells, Nattawadee Kanpipit, Challika Kaewborisuth, Teerapat Rungnirundorn, Nantawan Petcharat, Porntippa Lekcharoensuk

Turkish Journal of Veterinary & Animal Sciences

Bovine ephemeral fever (BEF) is a common febrile disease in cattle in tropical countries causing loss of milk production and reproductive performance. Recently, severe outbreaks have occurred in many countries in East Asia, the Middle East, and Southeast Asia, especially in the rainy season, while limited vaccines and diagnostic tools are available. Thus, the aim of this study was to produce a secreted, soluble form of BEFV G glycoprotein in insect cells for further applications. BEFV G gene expressing transmembrane-deleted G glycoprotein (G?TM) was constructed and cloned into the baculovirus vector. The recombinant G?TM protein was expressed in High5 insect …

The Influences Of Feeding Broilers On Graded Inclusion Of Sunflower Meal With Or Without Avizyme On Growth, Protein And Energy Efficiency, Carcass Traits, And Nutrient Digestibility, Mahmoud Alagwany, Adel Attia, Zenat Ibrahim, Mohamed Abd El-Hack1, Muhammed Arif, Mohamed Emam

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to investigate the substituting effect of sunflower meal (SFM) for soybean meal (SBM) with or without supplemental Avizyme on growth performance, protein and energy efficiency ratios, carcass traits, and nutrient digestibility of broiler chickens. A 4 × 2 factorial design experiment was conducted with four levels of SFM replacing SBM (0%, 25%, 50%, 75%) and two levels of supplemented Avizyme (0 or 0.1 g/kg diet). Results revealed that increasing SFM substitution for SBM up to 50% was associated with an increase in body weight (P < 0.01). Daily weight gain was statistically (P < 0.01) enhanced with increasing SFM up to 50% through 22 to 42 and 7 to 42 days of age. Feed conversion ratio improved (P < 0.01) in groups given SFM at 25% and 50% compared to the control. No carcass values were affected by SFM inclusion or Avizyme. The inclusion of 25% or 50% SFM increased (P < 0.01) protein efficiency at periods of 7?21 and 22?42 days of age. Energy efficiency ratio improved (P < 0.01) due to SFM incorporation at levels of 25% or 50% in all periods. The interaction effect between SFM and Avizyme was not significant on a majority of studied parameters. The digestion coefficients of crude fiber, N-free extract, and organic matter increased (P < 0.05 or P < 0.01) with increased levels of SFM. In conclusion, the incorporation of SFM as a substitute for SBM in broiler diets improved the growth performance of broilers. The use of SFM up to 50% without Avizyme supplementation could be recommended in broiler diets from 7 to 42 days of age.

Evaluation Of Real-Time Pcr, Mat, And Recombinant Lipl32-Based Elisa For The Diagnosis Of Canine Leptospirosis In A Disease-Endemic South Indian State, Kerala, Siju Joseph, Mangattumuruppel Mini, Vamshi Krishna Sriram, Ramachandran Ambily, Thazhathuveettil Aravindakshan, Sreedharannair Ajithkumar

Turkish Journal of Veterinary & Animal Sciences

Canine samples (n = 425) collected from cases of suspected leptospirosis over a period of 6 years were tested using the microscopic agglutination test (MAT), recombinant LipL32-based IgM and IgG ELISA, and real-time PCR (RT PCR). A prevalence of 69.41% was recorded by MAT. Among the different tests, IgG ELISA was observed to have maximum agreement with that of MAT (k-0.810), followed by IgM ELISA (k-0.762.). RT PCR had only slight agreement with MAT (k-0.156). However, the percentage positivity of RT PCR with samples with MAT titers of 1:100 and 1:200 were 76.47% and 85.96%, respectively, indicating the reliability of …

Cannabinoid Receptor Type 2 Agonist Jwh-133 Deteriorates The Liver Toxicity Induced By Cypermethrin*, Hi̇dayet Tutun, Emi̇ne Baydan

Turkish Journal of Veterinary & Animal Sciences

Cannabinoid receptor 2 (CB2) has a role in the pathology of some liver diseases. Cypermethrin (CYP) is a synthetic pyrethroid insecticide used commonly for the control of pests and vectors, and for protection of foodstuffs. In this study, the involvement of CB2 in CYP-induced liver damage in rats was investigated. CYP was applied to rats orally at a dose of 125 mg/kg bw per day for 14 days. CB2 agonist JWH-133 was administered by intraperitoneal injection at a dose of 3 mg/kg bw per day for the last 4 days of CYP toxicity. JWH-133 deteriorated the liver damage induced by …

The Effect Of Daily Milk Production On The Milk Composition And Energymanagement Indicators In Holstein?Friesian And Simmental Cows, Krzysztof Mlynek, Beata Gowiska, Ewa Salomonczyk, Jolanta Tkaczuk, Wojciech Stys

Turkish Journal of Veterinary & Animal Sciences

The present study investigated the effect of daily milk production on the content of crucial milk components, body condition, and the level of energy management parameters in the blood and milk in Holstein?Friesian (HF) and Simmental (SIM) cows. Within each breed, the animals were divided into two groups: the HF-L group was HF cows with daily milk production of ≤31 kg and the HF-H group was those with daily milk production of >31 kg. The SIM-L was SIM cows with daily milk production of ≤27 kg and the SIM-H group was those with daily milk production of >27 kg. The …

Effects Of Different Extenders And Additives On Liquid Storage Of Awassi Ram Semen, Ömer Varişli, Abdullah Taşkin, Numan Akyol

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode lactate (TL-HEPES), and Dulbecco?s phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg yolk, …

Comparison Of The In Vitro Anticancer Effect Of Habanero Pepper Extract Containing Capsaicin With That Of Pure Capsaicin In Selected Dog Neoplastic Cell Lines, Lukasz Adaszek, Olga Slabczy?Ska, Pawel Lyp, Dagmara Gadomska, Jerzy Zietek, Dorota Rozanska, Maciej Orzelski, Anna Smiech, Marta Staniec, Dorota Krasucka, Stanislaw Winiarczyk

Turkish Journal of Veterinary & Animal Sciences

The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer lines, DAN (fibroblasts isolated from osteosarcoma) and D-17 (epithelium cells of osteosarcoma obtained from pulmonary metastatic tumors), under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation (37 °C, 5% CO2), the fluid above the culture was removed, and diluted habanero pepper extract was added. The concentrations of capsaicin in …

Effect Of Steel Wire And Claw Size On The Sea Snail (Rapana Venosa) Catch In A Black Sea Beam Trawl Fishery, Muharrem Hakan Kaykaç, Aysun Gümüş, Mustafa Zengi̇n, Serdar Süer, Meli̇h Rüzgar, Ayşe Van, Zafer Tosunoğlu

Turkish Journal of Veterinary & Animal Sciences

The effects of the claw size used on the shoes of the traditional sea snail beam trawl and the effect of the steel wire used between the shoes on catching efficiency were investigated on the Samsun coast of the Black Sea. A traditional beam trawl with steel wire and with a claw length of 5.5 cm (T) was compared to modified beam trawls with steel wire (M1) and without steel wire (M2) with a claw size of 0.5 cm. The operations were performed as T-M1 and then T-M2 hauls using the dual parallel towing method. There was no significant difference …

Avian Salmonella Enteritidis Serovar Enteritidis With Clpp Deletion Is Attenuated And Highly Immunogenic In Chickens, Xuhua Ran, Zhiyuan Yang, Guagnxi Shao, Xiaobo Wen, Siguo Liu, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Salmonella enterica subsp. enterica serovars are leading causative agents of gastroenteritis in humans and numerous animal species worldwide. The ClpP from Salmonella enterica is responsible for regulation under various stress conditions. We were interested in the effect of ClpP deletion mutation on attenuation of Salmonella enterica subsp. enterica serovar Enteritidis (SE) in chickens. Therefore, SM-6/Δ ClpP , deletion of the ClpP gene from a wild-type SE-6 (SM-6) strain, was generated using the phage &lamda; Red homologous recombination. Compared with SM-6, SM-6/&DeltaClpP represented a retarded growth rate and lower concentration in LB broth with and without 5% NaCl or lower pH …