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Articles 481 - 510 of 2509
Full-Text Articles in Veterinary Medicine
Comparison Of Sperm Freezability And Fertility Among Hmong Black-Bone, Barred Plymouth Rock And Thai Native (Pradu Hang Dam) Chickens, Saennamphung Somtaw, Pornjit Sonseeda, Nalinee Tubtimtong, Yupin Phasuk, Thevin Vongpralub
Comparison Of Sperm Freezability And Fertility Among Hmong Black-Bone, Barred Plymouth Rock And Thai Native (Pradu Hang Dam) Chickens, Saennamphung Somtaw, Pornjit Sonseeda, Nalinee Tubtimtong, Yupin Phasuk, Thevin Vongpralub
The Thai Journal of Veterinary Medicine
Semen cryopreservation is the most useful method for the long-term storage of chicken genetic resources. The objective of this study was to compare post- thaw semen quality of Hmong Black- Bone to Barred Plymouth Rock and Thai native ( Pradu Hang Dam) chickens in terms of sperm motility, viability and fertility. A total of 9 males of each breed were used; semen collected from each cock was subsequently evaluated under a microscope. Semen samples were diluted with Schramm diluent, cooled down from 20– 25oC to 5oC and diluent plus DMF ( dimethylformamide) was added (6% DMF of final volume when …
Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan
Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This study determined the efficacy of recombinant HVT- NDV ( rHVT- NDV) vaccine simultaneously vaccinated with live vaccine at 1 day old and a booster vaccination with live vaccine at 10 days old against Asiatic, Genotype VII NDV in broiler chickens. The chickens were divided into 5 groups. The chickens in groups 1, 2 and 3 were vaccinated with rHVT- NDV vaccine and different live vaccines at 1 day old, and then finally received a booster vaccination at 10 days old. The chickens in groups 4 and 5 served as the positive and negative control groups, respectively. The chickens in …
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
This study aimed to determine plasmid profile of E. faecium and E. faecalis isolated from pigs, pig products and humans in Thailand-Laos border provinces. A total of 96 Enterococcus spp. including E. faecium (n=59) and E. faecalis (n=37) from pigs, pork and humans were included. All were determined for their antimicrobial susceptibilities and plasmid profile. Most isolates (96.9%) carried one to four plasmids with a molecular weight of 0.03-35 kb in E. faecium and 19-40 kb in E. faecalis. The 26 kb plasmid was most commonly detected. Variable plasmid profiles were defined, of which the most common pattern was, 23 …
Oxidative Stress And Antioxidant In Canine Cutaneous Mast Cell Tumors, Arayaporn Macotpet, Ekkachai Pattarapanwichien, Fanan Suksawat, Patcharee Boonsiri
Oxidative Stress And Antioxidant In Canine Cutaneous Mast Cell Tumors, Arayaporn Macotpet, Ekkachai Pattarapanwichien, Fanan Suksawat, Patcharee Boonsiri
The Thai Journal of Veterinary Medicine
Oxidative stress can result from either the excessive production of reactive oxygen species (ROS) or an impaired antioxidant system, or both. It causes damage to lipids, proteins and DNA. Therefore, oxidative stress may be involved in carcinogenesis, and is associated with many types of cancer in dogs. The objective of this study was to compare the levels of malondialdehyde, protein hydroperoxides, glutathione, retinol and alpha-tocopherol between dogs with mast cell tumors and clinically healthy controls. Blood samples were obtained from eighteen clinically healthy dogs and fourteen dogs with spontaneous mast cell tumors. Malondialdehyde and protein hydroperoxides levels were measured by …
Impact Of Feed Characteristics For Monitoring Afb1 Contamination In Commercial Concentrate Feeds Used In Small Holder Dairy Farms, Chiangmai, Thailand, Wasana Chaisri, Wantanwa Mongkon, Yoshigo Sugita-Konishi, Witaya Suriyasathaporn
Impact Of Feed Characteristics For Monitoring Afb1 Contamination In Commercial Concentrate Feeds Used In Small Holder Dairy Farms, Chiangmai, Thailand, Wasana Chaisri, Wantanwa Mongkon, Yoshigo Sugita-Konishi, Witaya Suriyasathaporn
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the impact of abnormal physical characteristics for monitoring aflatoxin B1 (AFB1) contamination in commercial dairy concentrate feeds. The study was conducted during March to May 2013 using 82 dairy farms in Chiang Mai province, Thailand. All feed samples were collected from new packages and their physical characteristics were subsequently determined. AFB1 concentrations in the samples were measured using the enzyme-linked immunosorbent assay (ELISA), and feed samples with AFB1 of more than 5 µg/Kg were determined as AFB1 contaminated samples. The impact of physical characteristics on AFB1 contamination were evaluated using a multiple …
Comparative Effect Of Dl-Methionine And Dl-Methionine Hydroxy Analogue Supplemented Diet On Productive Performance, Fat Accumulation And Lipid Profile In Blood Of Meat-Type Ducks, Chanwit Kaewtapee, Chaiyapoom Bunchasak
Comparative Effect Of Dl-Methionine And Dl-Methionine Hydroxy Analogue Supplemented Diet On Productive Performance, Fat Accumulation And Lipid Profile In Blood Of Meat-Type Ducks, Chanwit Kaewtapee, Chaiyapoom Bunchasak
The Thai Journal of Veterinary Medicine
The objective of this study was to compare the effect of DL-methionine (DLM) and DL-methionine hydroxy analogue (DL-MHA) supplementation on the growth performance, carcass quality, fat accumulation and lipid profile in blood of meat-type duck. Three hundred male Cherry Valley ducks were divided into 3 groups with 4 replications of 25 ducklings each. Each group was given a diet as follows: 1) basal diet, 2) basal diet + DLM, and 3) basal diet + DLMHA. The results show that dietary supplementation with DLM and DL-MHA increased body weight (P<0.01), feed intake (P<0.01) and pectoralis major (P<0.05), but decreased abdominal fat (P<0.01) when compared to the basal diet. Supplementation with DL-MHA also resulted in greater levels of triglycerides (P<0.05), low-density lipoprotein (P<0.05) and cholesterol (P<0.05) in the blood compared to the DLM group, in addition to greater levels of triglycerides (P<0.05) and high-density lipoprotein (P<0.05) compared to the basal diet. Furthermore, supplementation with DLM was shown to lower the level of plasma uric acid (P<0.05) compared to the basal diet. It is concluded that DL-MHA can promote the same productive performance in meat-type ducks as DLM, but the fat accumulation and lipid profile in blood was greater with the DL-MHA supplementation than with DLM. Furthermore, the underlying mechanism of different effects between DL-MHA and DLM on the lipid metabolism in ducks should be further investigated.
Emergence Of A Classical Variant Of Porcine Epidemic Diarrhea Virus Novel To Thailand Responsible For The Milder Clinical Disease In A Herd Previously Infected With A Pandemic Variant, Gun Temeeyasen, Anchalee Srijangwad, Thitima Tripipat, Angkana Tantituvanont, Dachrit Nilubol
Emergence Of A Classical Variant Of Porcine Epidemic Diarrhea Virus Novel To Thailand Responsible For The Milder Clinical Disease In A Herd Previously Infected With A Pandemic Variant, Gun Temeeyasen, Anchalee Srijangwad, Thitima Tripipat, Angkana Tantituvanont, Dachrit Nilubol
The Thai Journal of Veterinary Medicine
In 2014, porcine epidemic diarrhea (PED) outbreaks characterized by mild clinical disease and low preweaning mortality increased in frequency in Thailand. An outbreak investigation, based on the detection of the classical variant and the allowance of the herd owners, was conducted in 5 swine herds and 25 intestinal samples were submitted from herds with outbreaks of diarrhea for sequencing analysis. PEDV isolation and complete nucleotide sequences of the spike (S) glycoprotein were determined. The PEDV isolate EAS1 was identified. Phylogenetic analysis based on the full-length spike gene demonstrated that EAS1 belonged to the classical variant group with high nucleotide and …
Comparative Ultrasonographic And Computed Tomographic Images Of The Adrenal Glands Of Healthy Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Nan Choisunirachon
Comparative Ultrasonographic And Computed Tomographic Images Of The Adrenal Glands Of Healthy Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Nan Choisunirachon
The Thai Journal of Veterinary Medicine
Feline adrenal disorders that affect the size of the adrenal glands can be detected by diagnostic imaging including ultrasonography and Computed Tomography (CT). The objectives of this study were to compare the appearance of the adrenal gland in healthy cats between these two techniques and establish the reference values of the CT dimensions of the adrenal gland in cats. We found that there was no significant difference in the length of both adrenal glands between the two techniques but the cranial and caudal heights of the left adrenal glands and cranial height of the right adrenal gland obtained from the …
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
A 6-month-old French Bulldog had been presented with left eye blepharospasm. The puppy had been treated with topical antibiotics for weeks from the local veterinarian. However, blepharospasm still persisted. Ophthalmic examination from distance showed mild conjunctival hyperemia. Excessive tear was observed around the eyeball. Close ophthalmic examinations revealed prominent conjunctival blood vessels at the outer portion of the nictitating membrane. Clump of abnormal hairs were identified at the ventro-nasal region of the palpebral conjunctiva, next to eyelid margin. Schirmer tear test 1 level of the left eye was 25 mm wetness, while it was 16 on the right.
Investigation Into Toxin And Slime Genes In Staphylococci Isolated From Goat Milk And Goat Cheese In Southern Turkey, Sevda Pehlivanlar Onen, Zafer Cantekin, Faiza Mebkhout, Osman Aygun, Yasar Ergun
Investigation Into Toxin And Slime Genes In Staphylococci Isolated From Goat Milk And Goat Cheese In Southern Turkey, Sevda Pehlivanlar Onen, Zafer Cantekin, Faiza Mebkhout, Osman Aygun, Yasar Ergun
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the presence of Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from goat milk and cheese, as well as their toxin genes and slime genes. S. aureus and CNS isolates were subjected to polymerase chain reaction (PCR) analyses to determine the prevalence of enterotoxin (sea, seb, sec, sed, see), toxic shock syndrome (TSS) toxin (tst), exfoliative toxin (eta and etb) and slime genes (icaA and icaD). sec, sed and tst genes together were detected in 4 (33.3%), sec + tst genes in 2 (16.7%) and tst gene in 1 (8.3%) of the 12 …
Current Situation Of Neospora Caninum And Toxoplasma Gondii Infection Among Beef Cattle In Kanchanaburi, Ratchaburi And Nakhon Patom Provinces, Thailand, Ruenruetai Udonsom, Yaowalark Sukthana, Yoshifumi Nishikawa, Ragab M. Fereig, Charoonluk Jirapattharasate
Current Situation Of Neospora Caninum And Toxoplasma Gondii Infection Among Beef Cattle In Kanchanaburi, Ratchaburi And Nakhon Patom Provinces, Thailand, Ruenruetai Udonsom, Yaowalark Sukthana, Yoshifumi Nishikawa, Ragab M. Fereig, Charoonluk Jirapattharasate
The Thai Journal of Veterinary Medicine
Toxoplasmosis and neosporosis are parasitic diseases of livestock globally. In this study, we investigate the presence of Neospora caninum and Toxoplasma gondii in 250 beef cattle from Kanchanaburi, Ratchaburi and Nakhon Patom provinces, Thailand comparing with the results from 5 years earlier. The serological diagnosis for toxoplasmosis and neosporosis were performed by indirect ELISA technique using recombinant antigens TgGRA7 and NcSAG1, respectively. Furthermore, risk factors at the animal- and herd-levels were also evaluated. Seroprevalence of T. gondii, N. caninum and co-infections were 15.2%, 20.8% and 27.2%, respectively. In comparison to the results from 5 years earlier, seropositivity of N. caninum …
Novel Methods To Diagnose Pulmonary Hypertension In Dogs Assessed By Thoracic Radiography, Kawinthip Chanroon, Thanat Tuntipas, Promsin Sukpanich, Pawit Punyarit, Sirilak Disatian Surachetpong
Novel Methods To Diagnose Pulmonary Hypertension In Dogs Assessed By Thoracic Radiography, Kawinthip Chanroon, Thanat Tuntipas, Promsin Sukpanich, Pawit Punyarit, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
The objective of this retrospective study was to distinguish between dogs with pulmonary hypertension (PH) and non-PH by using parameters obtained from thoracic radiographs. Data of 72 dogs of small breeds aged more than 1 year old and weighing less than 10 kg were retrieved. Echocardiography was used as a gold standard to diagnose PH and separate the dogs into two groups: PH (n=15) and non-PH (n=57). Seven formulae were created from measurements on right lateral and ventrodorsal radiographic views. Five formulae from the ventrodorsal view provided an outcome that could differentiate PH and non-PH dogs. Two formulae are recommended …
The Effects Of Submaximal Exercise Training On Cardiovascular Functions And Physical Capacity In Dogs With Myxomatous Mitral Valve Disease, Saikaew Sutayatram, Chollada Buranakarl, Anusak Kijtawornrat, Kumpanart Soontornvipart, Pakit Boonpala, Prapawadee Pirintr
The Effects Of Submaximal Exercise Training On Cardiovascular Functions And Physical Capacity In Dogs With Myxomatous Mitral Valve Disease, Saikaew Sutayatram, Chollada Buranakarl, Anusak Kijtawornrat, Kumpanart Soontornvipart, Pakit Boonpala, Prapawadee Pirintr
The Thai Journal of Veterinary Medicine
Myxomatous mitral valve disease (MMVD), an important disease in senile small breed dogs, leads to deterioration of cardiovascular function and impairment of functional capacity resulting in poor quality of life. Submaximal treadmill running has been used as both cardiovascular training and testing worldwide in humans and recently in animals. The objectives of this study were to examine the effects of submaximal endurance training (ET) by treadmill running on safety, adverse events, cardiovascular adaptations, hematology, functional capacity, and the feasibility of submaximal treadmill running test and 6-minute walk test (6-MWT) in dogs with MMVD. Six adult beagle dogs with MMVD, stage …
Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech
Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Classical swine fever virus (CSFV) contributes to economic loss of swine production in endemic countries. Serum neutralization and enzyme-linked immunosorbent assay (ELISA) are serological tests commonly used for monitoring CSFV antibody status with serum samples. This experiment evaluated the detection of CSFV antibody in oral fluid samples in commercial ELISA test kit by in vitro study of negative oral fluid mixed with serum of known CSFV serum neutralizing (SN) titer and in vivo oral fluid samples obtained from experimental animals. Correlations of SN titer and S/P ratio of ELISA were observed with the in vitro oral fluid samples, indicating the …
Effects Of Coenzyme Q10 Supplementation On Cardiac Troponin I Level, Heart Rate Variability, And Echocardiographic Profiles In Canine With Myxomatous Degenerative Mitral Valve Disease: A Pilot Study, Kittipong Tachampa, Tuchakorn Lertwanakarn, Panupong Atchariyasakchai, Vichayanee Pumpitakkul, Supapatt Kireewan, Chollada Buranakarl
Effects Of Coenzyme Q10 Supplementation On Cardiac Troponin I Level, Heart Rate Variability, And Echocardiographic Profiles In Canine With Myxomatous Degenerative Mitral Valve Disease: A Pilot Study, Kittipong Tachampa, Tuchakorn Lertwanakarn, Panupong Atchariyasakchai, Vichayanee Pumpitakkul, Supapatt Kireewan, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
The clinical benefits of Coenzyme Q10 (CoQ10) have been well documented in heart failure patients. In veterinary medicine, however, the clinical benefits of CoQ10 have not been well established in dogs with heart diseases. This study aimed to determine the effects of CoQ10 supplementation on 1) preventing myocardial injury 2) cardiac function 3) autonomic balance in dogs with myxomatous degenerative mitral valve disease (MMVD). Thirteen dogs with MMVD at ACVIM stage C were recruited in this study. Dogs were further divided into group I (Body weight [BW] < 6 kg.; n = 7) and group II (BW ≥ 6 kg.; n = 6). Cardiac troponin I (cTnI), echocardiographic examination, and 2 hours of Holter recording were performed on Day 0 and after receiving CoQ10 at 100 mg/dog twice daily for 28 days. CoQ10 caused a reduction of cTnI level in 71% of the dogs. Median cTnI tended to cause tended to decline but not significantly. Systolic function [i.e. fractional shortening (FS) and ejection fraction (EF)] increased significantly by 8 % and 9% (P < 0.05), respectively in group I after CoQ10 supplementation. Other echocardiographic parameters were not altered in either group. HRV analysis revealed no change in autonomic function and balance. cTnI negatively correlated with FS (P < 0.01) and positively correlated with LF/HF ratio (P < 0.05). In conclusion, this study demonstrated the clinical benefits of CoQ10 supplementation in dogs with MMVD. CoQ10 improves cardiac function in small dogs with MMVD. Finally, supplementation of CoQ10 in dogs should be based on body weight.
Syrian Hamster (Mesocricetus Auratus) As An Animal Model For Haplorchis Taichui Infection: Biology, Morphology, And Pathology, Jiraporn Songsri, Thidarut Boonmars, Sirintip Boonjaraspinyo, Ratchadawan Aukkanimart, Pranee Sriraj, Parichart Boueroy, Nadchanan Wongchalee
Syrian Hamster (Mesocricetus Auratus) As An Animal Model For Haplorchis Taichui Infection: Biology, Morphology, And Pathology, Jiraporn Songsri, Thidarut Boonmars, Sirintip Boonjaraspinyo, Ratchadawan Aukkanimart, Pranee Sriraj, Parichart Boueroy, Nadchanan Wongchalee
The Thai Journal of Veterinary Medicine
Haplorchis taichui is a trematode that can mature in the small intestine of mammals and birds, including humans. Larval stages occur in various invertebrate and cold-blooded vertebrate hosts. The purpose of this study was to investigate parasite growth, including worm recovery rate, the body size of adult worms, eggs per worm and eggs per gram of feces and pathological changes of the small intestine in experimental hamsters. In this study, 6- to 8-weekold male Syrian hamsters (Mesocricetus auratus) were each infected with 200 H.taichui metacercariae and then sacrificed at weeks 1, 2, 3, 4, 6 and 8 post-infection. The small …
Development Of An Insoluble Hyaluronic Acid Membrane As An Effective Material For The Prevention Of Post-Thoracotomy Pleural Adhesions In Dogs, Akiko Uemura, Takashi Tanaka, Pinkarn Chantawong, Pitipat Kitpipatkun, Ryou Tanaka
Development Of An Insoluble Hyaluronic Acid Membrane As An Effective Material For The Prevention Of Post-Thoracotomy Pleural Adhesions In Dogs, Akiko Uemura, Takashi Tanaka, Pinkarn Chantawong, Pitipat Kitpipatkun, Ryou Tanaka
The Thai Journal of Veterinary Medicine
A novel membrane was previously found to be effective in preventing post-thoracotomy pleural adhesions in dogs. The aim of the present study was to verify the animals’ clinical condition, the biological fate of the residual membrane, the presence or absence of pleural adhesions, and any inflammatory reactions over a 10-week observation period following membrane implantation. The animals used were two male beagles. The experimental membrane, an insoluble hyaluronic acid membrane containing glycerin, was implanted under general anesthesia above the visceral pleura in the left pleural cavity of each dog after exposure to air for 30 min. A drainage tube was …
Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri
Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri
The Thai Journal of Veterinary Medicine
The peroxisome proliferator-activated receptors (PPARs) belong to nuclear receptor superfamily acting as transcription factors related to lipid and glucose metabolism. There are 3 subtypes including PPARα, PPAR, and PPAR. PPARα and PPAR activations can inhibit tumor cell proliferation and differentiation in many cell types. However, the expressions of these receptors in canine mammary tissue are still unknown. This study was performed to investigate the expressions of PPARα and PPAR in normal, benign, and malignant canine mammary tissues. Twenty four bitches at the age of 5-15 years old, which undergone unilateral mastectomy, were used in the study. Mammary tissues were sectioned …
Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li
Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li
The Thai Journal of Veterinary Medicine
Trichuris ovis (Nematoda: Trichuridae) is a widespread nematode that can infect a broad range of animal hosts, such as goats, sheep and oryx. This parasite is of vital importance as a pathogen, but its population genetics, molecular epidemiology and biology are still not well understood. In this study, T. ovis isolates were collected from goats in Hunan province, China, and sequences variation in cox1 gene were examined. A portion of cox1 gene from each collected adult T. ovis individual was separately amplified using the PCR method, and the amplicons were sequenced from both directions. The sequences of pcox1 gene were …
Long-Term Effect Of Low-Dose Imatinib Therapy For Pulmonary Hypertension Due To Chronic Degenerative Mitral Valve Disease In Six Dogs, Zi Ping Leong, Shinji Arita, Yoshiaki Hikasa
Long-Term Effect Of Low-Dose Imatinib Therapy For Pulmonary Hypertension Due To Chronic Degenerative Mitral Valve Disease In Six Dogs, Zi Ping Leong, Shinji Arita, Yoshiaki Hikasa
The Thai Journal of Veterinary Medicine
Six dogs were diagnosed with pulmonary hypertension (PH) secondary to chronic degenerative mitral valve disease (CDMVD) on echocardiography. Imatinib (3 mg/kg, every 24 hours, PO) was initiated without any changes to the background therapy to treat the PH. Follow-up evaluations at 1, 3, 5 and 6 months revealed substantial clinical and hemodynamic improvements. One dog showed deterioration after the imatinib withdrawal to necessitate a restart therapy. No side effects were observed throughout the 6-month treatment course. Low-dose imatinib may provide a promising treatment alternative for CDMVD-associated PH in dogs.
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
A nine-year-old, female Shih Tzu had presented with left buphthalmic eye. She had a history of chronic glaucoma (left eye), which had been treating with topical hypotensive medication for several months. Her right eye is normal.
Comparison Of Dried Blood Spot, Buccal Swab, Cloacal Swab And Feces As Dna Sources To Identify Avian Sexes By Pcr, Sariya Asawakarn, Intuorn Teeranuwat, Natchaya Watcharaprapapong, Nutta Siriwatchaiporn, Panithan Somsai, Mullika Kuldee, Gunnaporn Suriyaphol, Sirakarnt Dhitavat
Comparison Of Dried Blood Spot, Buccal Swab, Cloacal Swab And Feces As Dna Sources To Identify Avian Sexes By Pcr, Sariya Asawakarn, Intuorn Teeranuwat, Natchaya Watcharaprapapong, Nutta Siriwatchaiporn, Panithan Somsai, Mullika Kuldee, Gunnaporn Suriyaphol, Sirakarnt Dhitavat
The Thai Journal of Veterinary Medicine
Absence of sexual dimorphism makes it difficult to determine bird’s sex. A number of both invasive and noninvasive methods as a DNA template of sampling for avian sexing can be used. The aim of this study was to compare the efficacy of different sources of DNA for avian sexing by PCR. Samples were collected from dried blood spots, buccal swabs, cloacal swabs and feces. The highest success rate for sexing determination was from the dried blood spots, accounting for 100%, while those from the buccal swabs, cloacal swabs and feces were 74%, 75.47% and 29.17%, respectively. The non-urate fecal samples …
Comparison Of Gts And Rapd Assays To Characterize Thai Mycoplasma Gallisepticum Strains, Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo
Comparison Of Gts And Rapd Assays To Characterize Thai Mycoplasma Gallisepticum Strains, Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo
The Thai Journal of Veterinary Medicine
Mycoplasma gallisepticum (MG) is one of the important pathogens which have economic impact on the poultry industry worldwide. Molecular characterization is the effective method to study the relation among international strains and the epidemiology of MG transmission. In this study, 17 Thai MG strains were characterized by using 2 molecular assays including random amplified polymorphic DNA (RAPD) and gene-targeted sequencing (GTS) assays. Results showed that the RAPD assay could classify the Thai MG strains into 3 patterns.Most MG strains obtained from the same area were in the same pattern. Partial mgc2 gene was used to distinguish between the Thai MG …
Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh
Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh
The Thai Journal of Veterinary Medicine
The overall goal of the current study was to show possibly mechanisms by which mesenchymal stem cells (MSCs) or conditioned media (CM) exert immune-modulatory properties for amelioration of asthmatic changes. Male rats were classified into healthy (C) and ovalbumin-sensitized groups (S), which further were divided into three subgroups (n=6); rats given intratracheally PBS (CPT and SPT groups), CM (CST and SST groups) and MSCs (CCT and SCT groups). Two weeks post treatment, IL-13, miRNA- 133 and miRNA-155 transcripts, pathological injuries and the homing of MSCs into the lung parenchyma were evaluated. The local administration of CM and notably MSCs blunted …
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The main purpose of the present study was to investigate the localization of epithelial cadherin (E-cadherin) in the oviduct and uterine horn of swamp buffalo at the follicular and mid-luteal phases. E-cadherin is a transmembrane glycoprotein which plays a vital role in the mechanism of the maintenance epithelial architecture, cell growth and proliferation, cell to cell adhesion including cell to epithelium adhesion. Female reproductive tracts of swamp buffaloes (n=30) were collected from local abattoirs and categorized into follicular (n=15) and mid-luteal (n=15) phases. Tissue samples from the oviducts and uterine horns were regularly handled according to histological and immunohistochemical techniques. …
Therapeutic Use Of Sulfadimethoxine-Ormetoprim For Control Of Streptococcus Agalactiae Infection In Nile Tilapia (Oreochromis Niloticus) Fry, Nion Vinarukwong, Mintra Lukkana, Jan O. Berntsen, Janenuj Wongtavatchai
Therapeutic Use Of Sulfadimethoxine-Ormetoprim For Control Of Streptococcus Agalactiae Infection In Nile Tilapia (Oreochromis Niloticus) Fry, Nion Vinarukwong, Mintra Lukkana, Jan O. Berntsen, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
The therapeutic efficacy of sulfadimethoxine-ormetoprim (SDMX-OMP) against Streptococcus agalactiae (S. agalactiae) infection in tilapia (Oreochromis niloticus) was examined in tilapia fry. Fish (3+0.44 g) underwent 3-hour immersion challenge with 50% infective dose (2.58 x 106 CFU/ml) of S. agalactiae and were treated at 24 hours post challenge with daily dosages of 30 and 50 mg SDMX-OMP/kg body weight (BW) feeding medication for 7 days. The administration of medicated feed increased the survival of the infected tilapia fry. The survival rate of the challenged, non-medicated group was 51.25+4.79%. The fish which were challenged with S. agalactiae and received the 30 and …
Decreasing Duration Of Androgenic Hormone Feeding Supplement For Production Of Male Monosex In Tilapia (Oreochromis Spp.) Fry, Nion Vinarukwong, Mintra Lukkana, Janenuj Wongtavatchai
Decreasing Duration Of Androgenic Hormone Feeding Supplement For Production Of Male Monosex In Tilapia (Oreochromis Spp.) Fry, Nion Vinarukwong, Mintra Lukkana, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
The effects of an exogenous androgenic hormone on sex differentiation were examined in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.) fry. Tilapia aquaculture in Thailand commonly applies methyltestosterone at the dose of 80 mg/kg diet on the first feeding of fish fry for 21-28 days to achieve male-monosex crop. In this study, male sex reversal was induced with an alternative androgenic hormone, 17α- methyldihydrotestosterone (MDHT), at a dose of 80 mg/kg diet for shorter periods; 5, 10, 15, or 20 days. Microscopic examination of fish gonad stained with aceto-carmine was used to determine male and female fish fry …
Molecular Epidemiology And Antimicrobial Resistance Of Salmonella Spp. Isolated From Broilers And Pigs At Slaughterhouses In Thailand And China, Wenjing Yang, Dusadee Phongaran, Teerarat Prasertsee, Rendong Fang, Patchara Phuektes, Sunpetch Angkititrakul
Molecular Epidemiology And Antimicrobial Resistance Of Salmonella Spp. Isolated From Broilers And Pigs At Slaughterhouses In Thailand And China, Wenjing Yang, Dusadee Phongaran, Teerarat Prasertsee, Rendong Fang, Patchara Phuektes, Sunpetch Angkititrakul
The Thai Journal of Veterinary Medicine
This study aimed to compare the antimicrobial resistance patterns and DNA restriction patterns by PulsedField Gel Electrophoresis (PFGE) between Salmonella spp. isolated from broilers and pigs in Thailand and China. One hundred and sixty six isolates were collected from broilers and pigs at slaughterhouses in Khon Kaen, Thailand (n=106) between February to August 2017 and in Chongqing, China (n=60) between March to October 2015. Antimicrobial susceptibility of the isolates was determined using the disk diffusion method with nine antimicrobial agents. Genotypic diversity of the isolates used PFGE of Xbal-digested chromosomal DNA to determine. Of 166 Salmonella isolates, 37 serotypes were …
Long-Term Effects Of Repeated Oral Dose Of Ivabradine On Heart Rate Variability In Dogs With Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr, Nakkawee Saengklub, Vudhiporn Limprasutr, Anusak Kijtawornrat
Long-Term Effects Of Repeated Oral Dose Of Ivabradine On Heart Rate Variability In Dogs With Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr, Nakkawee Saengklub, Vudhiporn Limprasutr, Anusak Kijtawornrat
The Thai Journal of Veterinary Medicine
Degenerative mitral valve disease (DMVD) is the most common acquired cardiac disease in geriatric dogs leading to impaired cardiac autonomic activity and functions. This study aimed to evaluate the heart rate (HR), blood pressure (BP), myocardial oxygen consumption (MVO2) and heart rate variability (HRV) of dogs with DMVD stage B2 in response to long-term treatment with ivabradine, orally. Four beagles with naturally occurring DMVD stage B2 were instrumented with a 24-h Holter recorder to obtain electrocardiography and HRV, an oscillometric device to acquire blood pressure (BP), HR and myocardial oxygen consumption (MVO2) as assessed by rate-pressure product (RPP = HR …
Prevalence And Risk Factors For Canine Cognitive Dysfunction Syndrome In Thailand, Chutamas Benjanirut, Chanakarn Wongsangchan, Piyathip Setthawong, Wittanee Pradidtan, Suphanuch Daechawattanakul, Kris Angkanaporn
Prevalence And Risk Factors For Canine Cognitive Dysfunction Syndrome In Thailand, Chutamas Benjanirut, Chanakarn Wongsangchan, Piyathip Setthawong, Wittanee Pradidtan, Suphanuch Daechawattanakul, Kris Angkanaporn
The Thai Journal of Veterinary Medicine
Canine cognitive dysfunction syndrome (CDS) is a disease similar to Alzheimer’s in humans. This progressive neurodegenerative disease affects senior dogs, causes many behavioral changes and decreases welfare in both the dog and owner. Even with its high impact, many studies have shown CDS is underdiagnosed. The aims of this study were to investigate the prevalence of CDS in Thailand and to identify its risk factors. In this study, 622 senior dogs (seven years old and older) were randomly recruited. After an initial interview, 389 dogs were excluded from the study due to clinical and/or sensory impairment. Owners of the remaining …