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The Thai Journal of Veterinary Medicine

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Full-Text Articles in Veterinary Medicine

Efficacy Of Ultrasonic Dissection Device In Staphylectomy Surgery In Brachycephalic Airway Obstructive Syndrome (Baos) Dogs, Sunisa Thunyodom, Marissak Kalpravidh, Chanin Kalpravidh, Wijit Banlunara, Pasakorn Brikshavana Jun 2019

Efficacy Of Ultrasonic Dissection Device In Staphylectomy Surgery In Brachycephalic Airway Obstructive Syndrome (Baos) Dogs, Sunisa Thunyodom, Marissak Kalpravidh, Chanin Kalpravidh, Wijit Banlunara, Pasakorn Brikshavana

The Thai Journal of Veterinary Medicine

The ultrasonic scalpel instrument was compared with the conventional staphylectomy technique in elongated soft palate surgery in twenty brachycephalic airway obstructive syndrome (BAOS) canines. Patients were randomly divided into the conventional incisional and ultrasonic scalpel group. Operation time (minute), bleeding volume (ml), respiratory distress score, postoperative complications score, pain scores, epithelialization score and inflammatory score were assessed. Two weeks after surgery, soft palates tissues were biopsied from 7 conventional surgery dogs and 8 ultrasonic dissection device applied dogs. The operation time and bleeding volume in the conventional surgery group were significantly higher than that in conventional group (P<0.01 and P<0.001, respectively). In addition, the respiratory distress score was significantly higher in the conventional surgery group compared with the ultrasonic dissection device group on day 3 and day 28 post-operation (P<0.05). There were no significantly different postoperative complication scores, pain scores or inflammatory scores between two groups. The epithelialization score of the UD was significantly higher than that of the conventional group (P<0.05). The ultrasonic dissection device is user friendly, has decreased surgical time, complete hemostasis and provides fewer postoperative complications. The ultrasonic dissection device is recommended in high-risk patients such as those displaying senility, chronic inflammation, or bleeding disorders.


A Simple And Rapid Method For The Extraction Of Genomic Dna From Piglet Tails Without Using Proteinase K, Nathamon Yimpring, Komkrich Teankum, Prapat Suriyaphol, Nutthee Am-In, Gunnaporn Suriyaphol Mar 2019

A Simple And Rapid Method For The Extraction Of Genomic Dna From Piglet Tails Without Using Proteinase K, Nathamon Yimpring, Komkrich Teankum, Prapat Suriyaphol, Nutthee Am-In, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

Genomic DNA (gDNA) is a prerequisite for genomic research. However, the collection of blood samples from young animals for gDNA extraction is not practical. Since piglet tail docking is routinely performed in pig farms to prevent injury from tail biting, docking tails served as a practical source for gDNA in piglets. However, extraction of gDNA from piglet tails is difficult because of inelasticity and cartilage. The purpose of this study was to develop a rapid and simple method of isolating gDNA from piglet tails for downstream molecular purpose. Chopped piglet tails with ceramic beads in lysis buffer were vortex-homogenized prior …


Surgical Removal And Recurrence Of Leiomyosarcoma In A King Cobra, Ophiophagus Hannah, Taksa Vasaruchapong, Panithi Laoungbua, Lawan Chanhome, Wijit Banlunara Mar 2019

Surgical Removal And Recurrence Of Leiomyosarcoma In A King Cobra, Ophiophagus Hannah, Taksa Vasaruchapong, Panithi Laoungbua, Lawan Chanhome, Wijit Banlunara

The Thai Journal of Veterinary Medicine

An adult male wild-caught king cobra, Ophiophagus hannah, of unknown age was raised in captivity for seven years. The snake showed swelling at the caudal third of the body. Physical examination revealed an approximately 15x10 cm firm mass in coelomic cavity. Radiography showed soft tissue density causing complete obstruction of intestinal tract. The mass was surgically removed and diagnosed as leiomyosarcoma. The snake was monitored and the mass recurred two months post-surgery. A second surgical exploration showed severe adhesion of the mass to dorsal aorta, intercostal muscle, ribs and stomach. The snake was euthanized and the recurrent masses were diagnosed …


Accuracy Of Portable Human Glucose Meter (Accu-Chek® Performa) For Blood Glucose Measurement In Newborn Piglets, Morakot Nuntapaitoon, Sujin Sirisawadi, Sariya Asawakarn, Padet Tummaruk Mar 2019

Accuracy Of Portable Human Glucose Meter (Accu-Chek® Performa) For Blood Glucose Measurement In Newborn Piglets, Morakot Nuntapaitoon, Sujin Sirisawadi, Sariya Asawakarn, Padet Tummaruk

The Thai Journal of Veterinary Medicine

Blood glucose concentration is a significant postnatal survival indicator in newborn piglets. The standard procedure is difficult to perform under field conditions and requires a long period of time for processing. Portable glucose meters have been invented to yield a faster result and utilize a small blood sample. The present study aims to compare glucose concentration mea s ur ed using a portable human glucose meter ( Accu- chek® Performa) with the standard protocol. A total of 200 blood samples were collected from the umbilical cord of newborn piglets and were measured using the portable human glucose meter immediately after …


Effect Of Dexmedetomidine Combined With Pethidine On Minimum Alveolar Concentration Of Isoflurane In Dogs, Kanjana Vinyunantakul, Sumit Durongphongtorn, Marissak Kalpravidh Mar 2019

Effect Of Dexmedetomidine Combined With Pethidine On Minimum Alveolar Concentration Of Isoflurane In Dogs, Kanjana Vinyunantakul, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

The effect of dexmedetomidine alone and when combined with pethidine on a minimum alveolar concentration (MAC) of isoflurane was determined in 24 client-owned adult male dogs before castration. Dogs were assigned to Group 1 (n = 12; dexmedetomidine, 5 µg/kg IM) or Group 2 (n = 12; dexmedetomidine, 5 µg/kgand pethidine, 5 mg/kg IM) . Sedation and response to noxious stimulation (digit clamping) were evaluated at 15 minutes after drug injection. Then, anaesthesia was induced via a face mask with isoflurane in oxygen and maintained via an endotracheal tube with an end tidal concentration of isoflurane (E´ISO%) of 1%, at …


Epidemiologic Evaluation Of Feline Urolithiasis In Thailand From 2010 To 2017, Vachira Hunprasit, Pinit Pusoonthornthum, Lori Koehler, Jody P. Lulich Mar 2019

Epidemiologic Evaluation Of Feline Urolithiasis In Thailand From 2010 To 2017, Vachira Hunprasit, Pinit Pusoonthornthum, Lori Koehler, Jody P. Lulich

The Thai Journal of Veterinary Medicine

This cross-sectional study describes the epidemiological data of feline urolithiasis in Thailand. Information on 923 feline uroliths submitted from Thailand to the Minnesota Urolith Center between January 2010 and December 2017 was evaluated. The frequency of urolith types, the relationships between urolith type and breeds and sex, were analyzed. Calcium oxalate was the most common urolith (49.5%) and was commonly found in male cats (OR = 1.81; 95%CI: 1.39, 2.36) with a mean age of 6.3±3.2 years. Struvite was the second most common urolith (38%), more frequently found in females (OR = 1.46; 95%CI: 1.11, 1.91) with a mean age …


Transcriptome Analysis Of Zi Geese (Anser Cygnoides) Prehierarchical Follicles And Verification Of Related Genes In Arginine Metabolic Pathway, Yongfeng Sun, Lulu Liu, Chang Liu, Cornelius Tlotliso Sello, Huiyan Wu, Hongtao Lu, Chenguang Xu, Yujian Sui Mar 2019

Transcriptome Analysis Of Zi Geese (Anser Cygnoides) Prehierarchical Follicles And Verification Of Related Genes In Arginine Metabolic Pathway, Yongfeng Sun, Lulu Liu, Chang Liu, Cornelius Tlotliso Sello, Huiyan Wu, Hongtao Lu, Chenguang Xu, Yujian Sui

The Thai Journal of Veterinary Medicine

Arginine metabolism plays a pivotal role in the mammalian reproductive system by controlling various physiological processes of the reproductive organs including follicular development, ovulatory mechanisms and egg production. However little is known about the molecular mechanisms regulating arginine metabolism at early stages of folliculogenesis. The objective of the current study was to determine the arginine metabolic pathways in prehierarchical follicles of Zi geese through the Illumina Hiseq 2500 platform and their transcripts through the de novo assembly. In this study, five different stages of the geese prehierarchical follicles transcriptome were sequenced. A total of 31722729162 bp from 224929214 high quality …


Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu Mar 2019

Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the effect of a rapid heat-treatment method on Thai QX-like infectious bronchitis virus (IBV) and the efficacy on protection from the virus after heat treatment. QX-like IBV isolate THA46 was incubated at 56 oC. The incubated virus was collected every 5 min for up to 1 h and then they were inoculated into embryonated chicken eggs. At 6 days post-inoculation, allantoic fluid was harvested from the embryonated eggs which were inoculated with virus of the longest heat-treatment time that induced lesions in the embryos. That allantoic fluid was used in a subsequent …


Dual Infection Of A Thai Isolate Hp-Prrsv And The Pdmh1n1 2009 Siv In Weanling Pigs, Nattaphong Sangpratum, Roongtham Kedkovid, Yonlayong Woonwong, Chaitawat Sirisereewan, Sawang Kesdangsakonwut, Roongroje Thanawongnuwech, Jirapat Arunorat Mar 2019

Dual Infection Of A Thai Isolate Hp-Prrsv And The Pdmh1n1 2009 Siv In Weanling Pigs, Nattaphong Sangpratum, Roongtham Kedkovid, Yonlayong Woonwong, Chaitawat Sirisereewan, Sawang Kesdangsakonwut, Roongroje Thanawongnuwech, Jirapat Arunorat

The Thai Journal of Veterinary Medicine

After the introduction of the pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in Thai swine farms in 2009, a mild clinical outcome was observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in Thai swine farms, recently. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups; Negative, PRRSV, the SIV and Co-infection …


Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi Mar 2019

Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi

The Thai Journal of Veterinary Medicine

This cross-sectional study aimed to determine the prevalence of Trypanosoma evansi infection and its associated risk factors in camels in the Jazan region, southwestern Saudi Arabia. A total of 679 blood samples were collected from three local breeds of camel – Awadia, Awarkia and Sawahelle – in 11 randomly selected governorates of Jazan region. Blood samples were screened by Giemsa-stained blood film (GSBF) and polymerase chain reaction (PCR) techniques. The overall prevalence of T. evansi was 30.9% (210/679, CI: 27.6–34.5%) by PCR, whereas a prevalence of 22.2% (151/679, CI: 19.3–25.5%) was revealed by GSBF. The highest prevalence was identified in …


Combination Of Rock Inhibitor, Hypoxia And Melatonin Improved Differentiation Of Rabbit Induced Pluripotent Stem Cells Into Cardiac Progenitor Cells, Praopilas Phakdeedindan, Piyathip Setthawong, Mongkol Techakumphu, Theerawat Tharasanit Mar 2019

Combination Of Rock Inhibitor, Hypoxia And Melatonin Improved Differentiation Of Rabbit Induced Pluripotent Stem Cells Into Cardiac Progenitor Cells, Praopilas Phakdeedindan, Piyathip Setthawong, Mongkol Techakumphu, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Cardiac progenitors are a promising cell source for treating myocardial infarction. In this study, we improved the protocol for cardiac differentiation of rabbit induced pluripotent stem cells (iPSC) using Rho-associated protein kinase (ROCK) inhibitor (Y-27632), low O2 tension (hypoxia) and melatonin treatments. In experiment 1, the rabbit iPSCs were differentiated into cardiac cell fate via embryoid body (EB) formation with or without ROCK inhibitor. EB diameters were measured on day 3 of differentiation. In experiment 2, the EBs were plated on gelatin-coated dishes and further cultured in different oxygen tensions, hypoxia (5% oxygen) and normoxia (20% oxygen). The plated EBs …


Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun Mar 2019

Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun

The Thai Journal of Veterinary Medicine

Nicotine affected meiosis during oocyte maturation is associated with poor subsequent embryonic development. The aim of this study was to analyze the effects of nicotine exposure during in vitro maturation (IVM) on the outcomes of in vitro fertilization (IVF) and the expression of gene related development of oocytes and embryos. Immature (germinal vesicle, GV) oocytes were randomly cultured in IVM media containing nicotine at concentrations of 0.0 (control) 2.5, 5.0, and 10.0 mM. After culturing for 24 h, oocytes were assessed for their maturation rate, subjected to IVF and mRNA extraction for gene expression analysis using quantitative real time PCR …


Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang Mar 2019

Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang

The Thai Journal of Veterinary Medicine

Bovine mastitis has had a great impact on the economic losses of the dairy industry. Nowadays, environmental pathogens cause the main types of bovine mastitis, especially the streptococci. The aim of the study was to identify the major Streptococcus species causing bovine mastitis in Taiwan as well as to reveal their antimicrobial susceptibility profiles. By colony morphology identifying, Gram staining, and subculturing on Edwards agar, the preliminary identified streptococci were subjected to biochemical identification, latex agglutination tests and dick diffusion tests for susceptibility tests of 16 antimicrobial agents. Among a total of 157 strains, Streptococcus uberis was the most dominant …


Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho Mar 2019

Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho

The Thai Journal of Veterinary Medicine

The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …


Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan Mar 2019

Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the efficacy of the recombinant herpes virus of turkey-Newcastle disease virus (rHVT-NDV) vaccine against Newcastle disease virus (NDV) challenged in 14 days old broiler chickens. One hundred chickens were divided into 5 groups of 20 chickens each. Group 1 chickens received live NDV vaccine via the intra-nasal route. Group 2 chickens received rHVT-NDV vaccine by subcutaneous injection. Group 3 chickens, received both live NDV and rHVT-NDV vaccine. Groups 4 and 5 chickens did not receive any vaccine and served as positive and negative control groups. At 14 days old, all the chickens …


Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol Dec 2018

Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol

The Thai Journal of Veterinary Medicine

Nowadays, the lifespan of pets has been increased by improvements in veterinary practice but age-related disorders are also increasing. Several nervous system attenuations are well-known to be the result of aging and/or pathological lesions. In many cases, neuropathic pain (NP) can develop and cause a significant impact on the clinical outcome and quality of life. With the difficulty in identification of the specific cause of NP, most NP management is planned according to clinical manifestations and recommended trial therapy. The drug of choice for trial therapy seems to be amitriptyline, a tricyclic antidepressant with multiple effects on neurotransmitter receptors and …


Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit Dec 2018

Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Bone marrow was collected from 7 dogs and submitted to 3 different MSC isolation techniques (direct plating, red blood cell lysis and gradient density). The number of cells, expression of MSC markers and in vitro the osteogenic differentiation obtained from each technique were examined. In order to study in vivo the osteogenic capability of derived MSCs, non-union ulna lesions (n=3) were firstly induced by transplantation of a composite of polycarprolactone/ hydroxyapatite (PCL/HAp) scaffold experimentally on to a critical-size ulna bone defect. The MSCs were injected into the lesions. The non-union sites were examined by radiography, angiography and histology at 2, …


High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien Dec 2018

High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

Zika virus (ZIKV), chikungunya virus (CHIKV) and dengue virus (DENV) are emerging and re-emerging arboviral diseases. These viruses are transmitted to humans through the bites of Aedes mosquitoes. Recently, ZIKV infection has been described as an emerging disease in Thailand and many countries, especially in tropical and subtropical areas. Specific drugs and vaccines against these infections are unavailable; therefore, effective disease control relies on vector control measures only. To understand the transmission cycle of these viruses and mosquito vectors, this study is designed to investigate the natural infection of ZIKV, CHIKV and DENV in field-caught mosquitoes by molecular techniques. Adults …


Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon Dec 2018

Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The potential value of bolus tracking contrast enhanced computed tomography (CT) in the diagnosis of oral tumors in dogs was investigated by examining the relationship between contrast-enhanced CT image density and microvascularization. Among 20 dogs with oral tumors, aged 8 – 17 years, weight 3.6 – 40.0 kg, 13 dogs were suffering with melanoma (MM), 4 with squamous cell carcinoma (SCC), and 3 with fibrosarcoma. Based on contrast-enhanced CT scan, the time lag from the injection of contrast medium into the right cephalic vein until the contrast medium returned to the mid-cervical external jugular vein at the level of the …


A Preliminary Study On Diversity Of Midgut Microbiota In Aedes Aegypti (Linnaeus) And Culex Quinquefasciatus (Say) Collected From Bangkok, Thailand, Sonthaya Tiawsirisup, Jirinnut Sirijutalak, Mananya Sondang, Manunya Supol, Nut Ansusinha, Ranida Tuanudom, Channarong Rodkhum Dec 2018

A Preliminary Study On Diversity Of Midgut Microbiota In Aedes Aegypti (Linnaeus) And Culex Quinquefasciatus (Say) Collected From Bangkok, Thailand, Sonthaya Tiawsirisup, Jirinnut Sirijutalak, Mananya Sondang, Manunya Supol, Nut Ansusinha, Ranida Tuanudom, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Aedes aegypti and Culex quinquefasciatus are important mosquito vectors for many infectious diseases. A number of factors affect the vector competence of these mosquitoes for a specific pathogen. The bacteria harbored in the midgut are known to influence mosquito physiology and can alter the response to various pathogens. Bacteria from the midgut of Ae. aegypti and Cx. quinquefasciatus were cultured and identified using bacteriological and molecular techniques in this study in which two groups of mosquitoes were examined. The first group was laboratory- reared, and the second group were field- collected mosquitoes from Bangkok. Twelve bacterial genera ( i. e. …


Survival Time And Prognosis Factors In Hypertrophic Cardiomyopathy Cats With Congestive Heart Failure, Lalida Tantisuwat, Panduangjai Puangampai, Pornchanok Panpakdee, Thitinan Tangarsasilp, Sirilak Disatian Surachetpong Dec 2018

Survival Time And Prognosis Factors In Hypertrophic Cardiomyopathy Cats With Congestive Heart Failure, Lalida Tantisuwat, Panduangjai Puangampai, Pornchanok Panpakdee, Thitinan Tangarsasilp, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Hypertrophic cardiomyopathy (HCM) is a common myocardial disease in cats. Nowadays, there is no study determining the survival time of cats affected with HCM in Thailand. This study aimed to evaluate the survival time in HCM cats with congestive heart failure (CHF) in Thailand and to determine the factors associating with survival time. A retrospective study was performed in 47 HCM cats to analyze survival time and the association of survival time and factors including sex, age, breeds, weight, physical findings, the presence of complications, medication and echocardiographic findings. The data was analyzed by the Log-Rank test and displayed with …


Effectiveness Of Sentinel Rodents For Surveillance Of Exposure To Undocumented Bacterial Pathogens In Animal Research Facility, Shih-Keng Loong, Haryanti-Azura Mohammad-Wali, Nurul-Asma-Anati Che-Mat-Seri, Nur-Hidayana Mahfodz, Dzuzaini Mohd-Ghazali, Pooi-Fong Wong, Sazaly Abubakar Dec 2018

Effectiveness Of Sentinel Rodents For Surveillance Of Exposure To Undocumented Bacterial Pathogens In Animal Research Facility, Shih-Keng Loong, Haryanti-Azura Mohammad-Wali, Nurul-Asma-Anati Che-Mat-Seri, Nur-Hidayana Mahfodz, Dzuzaini Mohd-Ghazali, Pooi-Fong Wong, Sazaly Abubakar

The Thai Journal of Veterinary Medicine

The use of sentinel animals in animal research facilities is the cornerstone of animal health monitoring for exposure to natural pathogens of laboratory animals. This is because infection with these pathogens produces no overt signs of disease yet the infection may affect the outcome of research utilising these animals. One of the important pathogens includes Klebsiella pneumoniae, whose prevalence can be high in laboratory animals. This study sought to document the prevalence of K. pneumoniae and other undocumented bacterial pathogens in laboratory rodents for a 3- year period. K. pneumoniae and Chryseobacterium gleum were isolated from sentinel ICR mice housed …


Surgical Treatment For Tubular Colonic Duplication Communicating To Retroperitoneal Space In An Adult Dog, Nithida Boonwittaya, Piyathip Choochalermporn, Sunee Kunakornsawat, Teerapol Sirinarumitr Dec 2018

Surgical Treatment For Tubular Colonic Duplication Communicating To Retroperitoneal Space In An Adult Dog, Nithida Boonwittaya, Piyathip Choochalermporn, Sunee Kunakornsawat, Teerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Canine colonic duplication is a rare congenital anomaly, usually symptomatic in the first year of life, with the common signs of bowel obstruction. Most importantly, colonic duplication in an adult dog has not been reported. This case document reports a non-communicating tubular colonic duplication with concurrent retroperitoneal mass in a 3- year-old female crossbred dog, presented for further diagnosis and treatment of the abdominal mass. Without any remarkable clinical signs, physical examination noted only abdominal distension with mild discomfort. Ultrasonography demonstrated a large mass in the mid-caudal abdomen, a tubular mass appearance on the colonic wall and the presence of …


Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram Dec 2018

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

A 10-year-old female Pomeranian dog was presented to the Small Animal Teaching Hospital, Chulalongkorn University, on December, 2018, with a history of anorexia, frequent vomiting, depress and oliguria. Physical examination revealed that the dog was depressed but responsive to the stimuli. The dog was dehydrated (7%) with pale-pink mucous membrane. The capillary refilling time of 1.5 seconds with strong femoral pulse were detected. The respiratory rate was 65 breath per minute with normal sound while heart rate was approximately 60 beats per minute without cardiac murmur from auscultation using stethoscope. The systolic blood pressure was 120 mmHg. The blood collection …


What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon Dec 2018

What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A thirteen kilograms, 6-year-old, male, Shih Tzu dog.


Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang Dec 2018

Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang

The Thai Journal of Veterinary Medicine

The frozen goat semen process must wash seminal fluid from goat semen. A small dosage of alpha1 adrenoceptor antagonist or tamsulosin may reduce seminal fluid in goat. A latin square 4x4 was applied to twelve male goats to receive either normal saline (NSS), tamsulosin 3, 6 and 9 µg/kg intramuscular injection at one-week intervals. Semen collection by artificial vagina and libido scoring was performed within one-hour post injection. Semen quality and seminal fluid were evaluated after semen collection The results showed that none of the libido scores in the groups was different. Anejaculations occurred in 1 (8.3%), 3 (25%) and …


Effects Of Supplementing Varying Levels Of 1,3-Diacylglycerol (Dag) On Growth Performance, Apparent Nutrient Digestibility And Selected Fecal Microbial Populations In Growing Pigs, Wen Chao Liu, Kwan Sik Yun, In Ho Kim Dec 2018

Effects Of Supplementing Varying Levels Of 1,3-Diacylglycerol (Dag) On Growth Performance, Apparent Nutrient Digestibility And Selected Fecal Microbial Populations In Growing Pigs, Wen Chao Liu, Kwan Sik Yun, In Ho Kim

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the effects of 1,3-diacylglycerol (DAG) supplementation on growth performance, apparent digestibility and selected fecal microbial populations in growing pigs. A total of 80 crossbred pigs [(Landrace × Yorkshire) × Duroc] with an average initial body weight (BW) of 27.52 ± 0.20 kg were used in a 6 week feeding trial. Pigs were allotted to 4 dietary treatments based on their initial BW and sex (5 replications; 4 pigs per pen with 2 barrows and 2 gilts). Dietary treatments included: 1) CON, basal diets; 2) CON+0.075% 1,3-DAG; 3) CON+0.10% 1,3-DAG; 4) CON+0.15% …


Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy Dec 2018

Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy

The Thai Journal of Veterinary Medicine

Kaempferol, myricetin and quercetin are abundant flavonols in edible fruit and vegetables. Much previous data has shown the beneficial effects of these flavonols in cancer treatments. Thus, the cytotoxic effects on rhesus monkey kidney epithelial cells (LLC-MK2) and the anti-proliferative effects on human cervical cancer (HeLa) cells through total ERK protein expression of kaempferol, myricetin and quercetin have been investigated. The cytotoxic assay at 24h revealed the high safety of three flavonols on LLC-MK2 cells. Kaempferol (1-1000 µM) did not have any significant effect on the viability of these normal epithelial cells. The cytotoxicity of myricetin and quercetin was 5 …


Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron Dec 2018

Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron

The Thai Journal of Veterinary Medicine

Fibrin glue has been widely used for controlling hemorrhage and sealing tissue in surgery. The purpose of this study was to evaluate the hemostatic efficacy of sheep-derived fibrin glue. Six Marino sheep were used to collect concentrate fibrinogen preparation by the ammonium sulfate precipitation method. Crossbred pigs (n=6) were used to evaluate hemostatic efficacy by monitoring whole blood clotting time by the glass slide method, bleeding time and bleeding quantity during liver biopsy. Mean whole blood clotting time on a glass slide started at 201.1±90.47 and 4.43±3.73 seconds, and was completed at 447.83±63.77 and 31.93±4.28 seconds in control and treatment …


The Potential Use Of Phytoestrogen Containing The Herb, Pueraria Mirifica, For Bone Healing In Osteoporotic Monkeys, Donlaporn Kittivanichkul, Nan Choisunirachon, Kumpanart Soontornvipart, Suchinda Malaivijitnond Dec 2018

The Potential Use Of Phytoestrogen Containing The Herb, Pueraria Mirifica, For Bone Healing In Osteoporotic Monkeys, Donlaporn Kittivanichkul, Nan Choisunirachon, Kumpanart Soontornvipart, Suchinda Malaivijitnond

The Thai Journal of Veterinary Medicine

Within the last decade, the positive effects of Pueraria mirifica on osteoporosis have been established in rats and monkeys. However, the effect of P. mirifica on bone healing has not been elucidated. In this study, post-menopausal osteoporotic monkeys were subjected to an iliac crest biopsy, divided into two groups (5 animals/group), and fed daily with standard monkey diet alone (PMP0 group) or mixed with 1,000 mg/kg body weight of P. mirifica powder (PMP1000 group) for 16 months. The progression of the bone healing was continuously assessed by X-ray radiography, and three individuals from each group were selected for a computed …