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The Thai Journal of Veterinary Medicine

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Full-Text Articles in Veterinary Medicine

Molecular Evidence Of Cryptosporidiosis In Farmed Blue Foxes (Vulpes Lagopus) And Raccoon Dogs (Nyctereutes Procyonoides) In Heilongjiang, China, Shakeel Hussain, Lixin Wang, Zhijun Hou Jun 2021

Molecular Evidence Of Cryptosporidiosis In Farmed Blue Foxes (Vulpes Lagopus) And Raccoon Dogs (Nyctereutes Procyonoides) In Heilongjiang, China, Shakeel Hussain, Lixin Wang, Zhijun Hou

The Thai Journal of Veterinary Medicine

Cryptosporidium, an important zoonotic protozoan, causes Cryptosporidiosis in humans as well as in animals. This study aimed to identify Cryptosporidiosis in farmed fur animals (Blue Fox and Raccoon Dogs). A total of 283 specimens (223 foxes, 60 Raccoon dogs) were screened through nested-PCR by targeting the actin gene. The results showed the overall prevalence of cryptosporidiosis was 9.1% where the prevalence in foxes and raccoon dogs was 9.1% and 8.3%, respectively. The prevalence in males (15%) was significantly higher than that in females (5.7%). The findings of this study portrayed new knowledge that foxes and raccoon dogs are likely to …


Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim Jun 2021

Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 6-year-old neutered male Siamese cat was presented with an acute onset of vomiting, anorexia and lethargy lasting for four days. An abdominal mass was palpated on physical examination. The cat showed severe anemia, elevated liver enzymes and hypoalbuminemia. Abdominal ultrasonography revealed a suspected hepatic mass without blood flow within the affected region. Computed tomography was performed to better visualize the abdominal cavity and blood flow. Prompt diagnosis of torsion of the left lateral liver lobe was made and immediate surgery for liver lobectomy was performed. Histopathological examination revealed no evidence of neoplasia. The cat remained clinically normal during the …


Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri Jun 2021

Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri

The Thai Journal of Veterinary Medicine

A 19-year-old polo pony presented with mild lameness alongside a lateral protuberance of the right tarsus. Radiographic findings revealed various defects, including an osteochondral lesion of the medial trochlear ridge of the talus, a sclerotic lesion of the tarsometatarsal joint, a radiolucent area on the distal border of the fourth tarsal bone and an enthesophyte at the proximolateral aspect of the metatarsal bone. Ultrasonography demonstrated the interesting finding of synovial sheath thickening of the lateral extensor tendon with synovial fluid accumulation. In addition, the presence of neutrophils and a granular mucinous background, according to cytological analysis of the fluid, indicated …


Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul Jun 2021

Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

Considering the impact of Salmonella in turtles and the role of turtles as a source of Salmonella outbreaks in humans, knowledge of Salmonella status in turtles is important. A cross-sectional study was conducted to investigate the prevalence, risk factors and antimicrobial resistance of Salmonella infections in turtles. Cloacal swabs were collected from 378 turtles in Turtle Villages located in Khon Kaen province of northeastern Thailand between January 2018 and April 2019. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. The overall prevalence of Salmonella in the turtles was 37.57% (142/378). Habitat, season and sex were …


Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr Jun 2021

Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Porcine epidemic diarrhea virus (PEDV) is the causative agent of severe watery diarrhea in suckling piglets. PEDV outbreaks have been consistently reported in Europe, Asian countries and Thailand. The recombinant protein, especially the spike (S) protein, from field strain PEDV could be used to develop a sensitive diagnostic test or an effective vaccine. In this study, the recombinant S2 domain protein from the PEDV Thai isolate was expressed using the E. coli expression system. The full length S2 domain of the S gene of the PEDV G2b Thai isolate was amplified using a PCR technique and was cloned into the …


Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus, Li Yi, Weiping Yang, Jinpeng Li, Shaohui Wang, Yang Wang Jun 2021

Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus, Li Yi, Weiping Yang, Jinpeng Li, Shaohui Wang, Yang Wang

The Thai Journal of Veterinary Medicine

Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many animal species. Studies aimed at developing safe and effective vaccines against SEZ infections are thus of great interest. SeseC_00396 was a novel protein identified in the previous study. In this study, recombinant SeseC_00396 of SEZ was expressed and showed a strong immunoreactivity with mini-pig convalescent sera. Studies in mice have revealed that the recombinant protein induced a marked antibody response which was associated with protection against SEZ infection. The hyperimmune sera against SeseC_00396 could efficiently kill the bacteria. In addition, it was also found that …


Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Eric Lim Teik Chung, Azlan Che-Amat, Mohd Lila Mohd-Azmi Jun 2021

Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Eric Lim Teik Chung, Azlan Che-Amat, Mohd Lila Mohd-Azmi

The Thai Journal of Veterinary Medicine

Outbreaks of haemotropic Mycoplasma ovis cause haemolytic anaemia with decreased production outcomes and mortality in sheep and goats worldwide but there is a lack of published data on the epidemiology of M. ovis in Malaysia's small ruminant flocks. This preliminary study investigates the prevalence and risk factors of Mycoplasma ovis infection in selected smallholder farms in Negeri Sembilan state. A total of 341 blood samples was randomly collected from 5 farms between January and December 2019. The farmers also completed a structured questionnaire to provide farm management data and environmental information. Giemsa stained blood smears were examined microscopically to detect …


Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha Jun 2021

Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha

The Thai Journal of Veterinary Medicine

Two hundred and fifty one-day old broiler chicks were used to evaluate the effect of black seeds and/or turmeric on performance and immune response to Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV) and Infectious Bursal Disease virus (IBDV) in broiler chickens of six weeks of age. The birds were divided into five groups (50 birds in each) with 5 replicates (10 birds each). Birds in the first group were fed a basal diet and served as a control. Birds of groups 2-5 were fed a basal diet mixed with black seed (20g/ kg basal diet), turmeric (20g/ kg basal …


The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect, Latif Emrah Yanmaz, Ugur Ersoz, Sitkican Okur, Betul Apaydin Yildirim, Selim Comakli, Yavuz Selim Sağlam, Elif Dogan, Ozhan Karatas, Ferda Turgut, Zafer Okumuş Jun 2021

The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect, Latif Emrah Yanmaz, Ugur Ersoz, Sitkican Okur, Betul Apaydin Yildirim, Selim Comakli, Yavuz Selim Sağlam, Elif Dogan, Ozhan Karatas, Ferda Turgut, Zafer Okumuş

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effectiveness of low-level laser therapy (LLLT) in the healing of experimentally created unicortical defect (UCD) of rat femurs. Twenty-four adult Wister rats were randomly divided into 2 groups: UCD and UCD+LLLT. The animals were subdivided into 3 groups (each group had 4 animals) and then they sacrificed at day 8, 15 and 22 for radiological, pathological and biochemical analysis. Bone healing was significantly different between the groups at day 15 and 22 (P < 0.05), while bone formation was significantly increased in the UCD+LLLT group at day 22 compared to the UCD group (P < 0.05). On histopathological examination, significant difference was found between the UCD and UCD+LLLT groups on the 22nd day (P < 0.05) while there was no difference on the 8th and 15th day. In the UCD group, the plasma Alkaline phosphatase activity decreased on the 15th and 22nd days, Calcium on the 15th day and Phosphorus on the 8th and 22nd days compared to the UCD+LLLT group. In addition, Glutathione levels, Plasma Catalase, Glutathione Peroxidase and Superoxide Dismutase activity significantly reduced in the UCD group compared with UCD+LLLT on the 8th, 15th and 22nd day (P < 0.05). In conclusion, LLLT applied at a dose of 904µm accelerates fracture healing in UCD of a rat’s femur. However, this effect shows an increase after the 22nd day of LLLT application.


Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee Jun 2021

Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee

The Thai Journal of Veterinary Medicine

Salmonella is one of the major foodborne pathogens, often as the result of pork consumption. Currently, with the situation of antimicrobial resistance, organic farming has been suggested as an alternative for healthier options. However, there is little evidence to support this. In this study, we investigated the occurrence of Salmonella circulating in local organic pig farms in northern Thailand and typed isolated clones to better understand the population structure and transmission dynamics of the underlying Salmonella contamination. In total, 112 samples from 11 organic pig farms were processed from October to December 2018. Salmonella was detected in 9 targeted farms. …


Possibility Of Trypsin Treated Dog Erythrocytes As Universal Blood: Evaluation By Ftir Microspectroscopy, Pornphimon Metheenukul, Kanjana Thumanu, Thanit Metheenukul Jun 2021

Possibility Of Trypsin Treated Dog Erythrocytes As Universal Blood: Evaluation By Ftir Microspectroscopy, Pornphimon Metheenukul, Kanjana Thumanu, Thanit Metheenukul

The Thai Journal of Veterinary Medicine

A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopy was used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+ canine erythrocytes. Production of canine DEA 1.1- erythrocytes was achieved by treating DEA 1.1+ red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1- erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR …


A Rapid Fecal Immunochemical Test For Gastrointestinal Bleeding Detection In Cynomolgus Macaques (Macaca Fascicularis), Taratorn Kemthong, Krittiga Sapkanarak, Piyachada Rounmakok, Thipchompoo Sing-Ayudthaya, Lalitta Suriya-Arunroj, Florian Föger, Martin Werle, Suchinda Malaivijitnond Jun 2021

A Rapid Fecal Immunochemical Test For Gastrointestinal Bleeding Detection In Cynomolgus Macaques (Macaca Fascicularis), Taratorn Kemthong, Krittiga Sapkanarak, Piyachada Rounmakok, Thipchompoo Sing-Ayudthaya, Lalitta Suriya-Arunroj, Florian Föger, Martin Werle, Suchinda Malaivijitnond

The Thai Journal of Veterinary Medicine

We used the Fecal Immunochemical Occult Blood Test (FIT) kit, a lateral flow immunochromatographic assay specific for human hemoglobin, on cynomolgus monkeys (Macaca fascicularis). We aimed to employ this test to detect gastrointestinal injury in captive animals at the National Primate Research Center of Thailand-Chulalongkorn University (NPRCT-CU). The cross-reactivity and sensitivity of the test for monkey blood were determined and compared with the human blood. The anti-human hemoglobin antibody of the FIT kit reacted with the monkey blood in a similar way as it did with humans and the intensity (T/C ratio) values between the two data sets closely correlated …


Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines, Chia-Tang Chen, Chiao-Hsu Ke, Hsin-Pei Weng, Chi-Hsun Liao, Chen-Si Lin Jun 2021

Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines, Chia-Tang Chen, Chiao-Hsu Ke, Hsin-Pei Weng, Chi-Hsun Liao, Chen-Si Lin

The Thai Journal of Veterinary Medicine

Canine mammary tumors (CMT) and human breast cancer (HBC) are the most frequent cancers in both female dogs and women worldwide, and they share an urgency for the development of effective markers. Overexpression of kynurenine 3-monooxygenase (KMO), a secondary enzyme of the kynurenine pathway, has been shown to indicate poor prognosis in CMT patients. To further investigate the role of KMO in mammary tumor development, we first analyzed the significant pathways related to KMO from the Database for Annotation, Visualization and Integrated Discovery (DAVID). The results revealed that several Kyoto Encyclopedias of Genes and Genomes (KEGG) pathways might correlate with …


The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam Jun 2021

The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam

The Thai Journal of Veterinary Medicine

A three-year-old Saanen buck presented with a complaint of stranguria for 24 hours. A physical examination revealed that the perineal urethra was turgid, swollen, and tender, and the urinary bladder was distended. The goat was diagnosed with obstructive urolithiasis. A percutaneous tube cystotomy technique was performed to relieve progressive urinary bladder distension. Using a human suprapubic urinary bladder catheter system, the technique was performed twice due to the recurrence of the blocked urethra. Following drainage of the urine, all vital parameters returned to normal. After surgical cystotomy, an intraoperative observation revealed the urinary bladder was filled with white sandy urolith, …


Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn Jun 2021

Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objectives of this study were to determine the differences in the expression of heat shock protein, and steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including cool (Jan to Feb), summer (Mar to Apr) and rainy (May to Jul) seasons. Ambient temperature and relative humidity recorded on the day before oocyte collection were used to classify environment groups as: low temperature and low …


Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk Jun 2021

Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk

The Thai Journal of Veterinary Medicine

In a tropical environment, sow productivity is strongly associated with boar sperm production. Regular monitoring of semen quality in boars that produce semen for artificial insemination is important. The objective of the present study was to investigate the boar sperm production in an evaporative cooling system in a tropical environment in association with breed and season. A total of 2878 ejaculates from 68 boars were collected from a boar stud in the western region of Thailand. The breeds consisted of 22 Duroc, 22 Yorkshire, 18 Landrace and six hybrid boars. Seasons were classified as cool, hot or rainy. Semen volume, …


Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds, Ulia Renfelia Baysi, Pramote Paengkoum, Wanwisa Ngampongsai, Chaiyawan Wattanachant, Pin Chanjula, Pitunart Noosen Jun 2021

Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds, Ulia Renfelia Baysi, Pramote Paengkoum, Wanwisa Ngampongsai, Chaiyawan Wattanachant, Pin Chanjula, Pitunart Noosen

The Thai Journal of Veterinary Medicine

This study was conducted to investigate the effect of oil palm frond (OPF) inclusion in total mixed ration (TMR) diets on nutrient digestibility, fermentation pattern, blood biochemical level and inflammatory response in nulliparous dairy goats. Three nulliparous female dairy goats (Saanen x Thai native, 33.88±1.78 kg) were randomly assigned in a 3x3 Latin square design. The diets consisted of 0% OPF (CON), 20% OPF (LOPF) and 40% OPF (HOPF) on a dry matter (DM) basis. The inclusion of OPF in the diets significantly increased the DM and nutrient intake of the dairy goats (p<0.05). There was no significant difference in the digestibility of DM, organic matter and crude protein of LOPF and HOPF, (p>0.05) but those were significantly higher …


Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen Jun 2021

Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …


Effects Of Medium-Chain Free Fatty Acids On Performance, Some Biochemical Parameters And Meat Fatty Acids Profile Of Broiler Chickens, Mehmet Demi̇rci̇, Mehmet Başalan Jun 2021

Effects Of Medium-Chain Free Fatty Acids On Performance, Some Biochemical Parameters And Meat Fatty Acids Profile Of Broiler Chickens, Mehmet Demi̇rci̇, Mehmet Başalan

The Thai Journal of Veterinary Medicine

In this study, it was aimed to investigate the effects of the addition of caprylic (octanoic, C8:0), capric (decanoic, C10:0) and lauric (dodecanoic, C12:0) acids from medium-chain free fatty acids to broiler diets. A total of 120 one-dayold male broiler chicks (Ross 308) were used and the study was conducted on 4 main groups of broilers; one control and three trials. The birds in the control group was fed an unadulterated basal diet and those in the experimental groups were fed with 0.2% of caprylic, capric and lauric acids (in addition to a basal diet) respectively. In the study, there …


A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats, Hsu-Hsun Lee, Ting-Sheng Lin, Lee-Shuan Lin, Cheng-Shu Chung, Shyh-Shyan Liu, Huan-Yu Hsu, Ching-Hou Ma, Jai-Wei Lee Jun 2021

A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats, Hsu-Hsun Lee, Ting-Sheng Lin, Lee-Shuan Lin, Cheng-Shu Chung, Shyh-Shyan Liu, Huan-Yu Hsu, Ching-Hou Ma, Jai-Wei Lee

The Thai Journal of Veterinary Medicine

External fixation is commonly used to treat long bone fractures in goats. It provides good enough stabilization on fractures to improve healing, minimizes soft tissue injury and only causes mild obstruction of the blood supply around the wounds. However, the large size and heavy weight of traditional external fixators (TEFs) cause them leave something to be desired. In this study, the feasibility of a novel custom-made external fixator (CMEF) for treating femoral fractures in goats was evaluated. Three one-year-old Anglo-Nubian female goats were subjected to experimental femoral fractures to their left femur with a CMEF installed for fixation. To evaluate …


Generalized Enamel Hypoplasia In A Dog: A Case Report, Issarin Sittijarusub, Panithi Sukho, Patchamon Supannawapat, Namphung Suemanotham Jun 2021

Generalized Enamel Hypoplasia In A Dog: A Case Report, Issarin Sittijarusub, Panithi Sukho, Patchamon Supannawapat, Namphung Suemanotham

The Thai Journal of Veterinary Medicine

A one-year-old, spayed female Welsh Corgi dog referred to the dental and oral clinic with chief complaint of apparent pitted, rough teeth surfaces, stained a tan to dark brown color. The dog was diagnosed with diffused enamel hypoplasia with no evidence of endodontic disease or malformation of tooth root. A restorative procedure with nanohybrid composite resin was performed. After treatment, the dog showed a good outcome with a smooth tooth surface, restorative material replaced to cover the previous defect and no evidence of pain or discomfort. Even though enamel hypoplasia is a rare, this condition remains an important problem that …


Case Report: Peripheral Iridectomy And Sclerectomy For Uveitic Glaucoma In A Labrador Retriever, Natthanet Sritrakoon, Aree Thayananuphat Jun 2021

Case Report: Peripheral Iridectomy And Sclerectomy For Uveitic Glaucoma In A Labrador Retriever, Natthanet Sritrakoon, Aree Thayananuphat

The Thai Journal of Veterinary Medicine

A 4-year-old male Labrador retriever dog presented with a history of conjunctivitis OU and mild buphthalmos OS for one week. Ophthalmic examination revealed corneal edema, conjunctivitis, iritis, aqueous flare, posterior synechia, fibrin on the anterior lens capsule, increased intraocular pressure (IOP =30 mmHg OD; IOP = 48 mmHg OS), weak positive menace response, brisk positive dazzle reflex and negative pupillary light reflex OU. The iridocorneal angle could not be visualized due to corneal edema. Physical examination, hematology and blood chemistry results were normal. Both eyes were diagnosed as having glaucoma and uveitis. Topical prednisolone acetate, atropine sulfate, brinzolamide and timolol …


Clinical Evaluation Of The Accuracy Of Foetal Radiographic Findings In Parturition Date Prediction Of Small Size Dogs, Panthipa Borikappakul, Nardtiwa Chaivoravitsakul, Rampaipat Penchome, Suppawiwat Ponglowhapan Mar 2021

Clinical Evaluation Of The Accuracy Of Foetal Radiographic Findings In Parturition Date Prediction Of Small Size Dogs, Panthipa Borikappakul, Nardtiwa Chaivoravitsakul, Rampaipat Penchome, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Ultrasonographic measurements of extra-foetal or foetal structures have been reported as an accurate means of predicting the date of parturition in the bitch. However, for many years, radiography has been clinically useful for pregnancy diagnosis, determining the number of foetuses and prolonged foetal death. Guidelines for estimating the canine parturition date based on foetal skeletal mineralization in relation to gestational age and preovulatory LH surge have been applied in small animal practice. The aims of this retrospective study were to evaluate the accuracy of parturition date prediction using foetal radiographic findings in small size dogs (1-10 kg), and to determine …


Pharmacokinetics Of Oxfendazole Nanosuspension In Sheep, Yuzhu Sun, Xiaofang Wang, Aoxue Zhang, Bao Zhang, Liwei Shao, Yue Liu, Haiting Wang, Wanhe Luo, Dongmei Chen, Shuyu Xie Mar 2021

Pharmacokinetics Of Oxfendazole Nanosuspension In Sheep, Yuzhu Sun, Xiaofang Wang, Aoxue Zhang, Bao Zhang, Liwei Shao, Yue Liu, Haiting Wang, Wanhe Luo, Dongmei Chen, Shuyu Xie

The Thai Journal of Veterinary Medicine

The pharmacokinetics of our previous prepared oxfendazole nanosuspension was studied in sheep to understand its enhancement of bioavailability. Ten sheep were randomly divided into two groups, each of which received a singledose (5mg/kg) oral oxfendazole nanosuspension and oxfendazole granules. After intragastric administration, oxfendazole rapidly reached peak concentrations of 11.25 μg/mL and 5.50 µg/mL at 5.6 h and 6.6 h in the nanosuspension group and granules group and the concentrations gradually reduced to below the detection limit at 96 h and 72h, respectively. The main pharmacokinetic parameters of oxfendazole after administration to the sheep: The Tmax, Cmax, AUClast, MRTlast, T1/2 and …


Evaluation Of Dermal Toxicity Study Of Ethanolic Extract Of Morinda Citrifolia Fruit In Spraque Dawley Rats, Farhan Hanif Reduan Mohd, Rosly Shaari Mohd, Nurul Syahirah Ahmad Sayuti, Abdullahi Aliyu, Noordin Mohamed Mustapha, Md Zuki Abu Bakar, Shanmugavelu Sithambaram, Hazilawati Hamzah Mar 2021

Evaluation Of Dermal Toxicity Study Of Ethanolic Extract Of Morinda Citrifolia Fruit In Spraque Dawley Rats, Farhan Hanif Reduan Mohd, Rosly Shaari Mohd, Nurul Syahirah Ahmad Sayuti, Abdullahi Aliyu, Noordin Mohamed Mustapha, Md Zuki Abu Bakar, Shanmugavelu Sithambaram, Hazilawati Hamzah

The Thai Journal of Veterinary Medicine

Plant-based preparations are widely known to complement dermatological therapy, however toxic profiles of these plants are lacking and limited. To investigate the toxicity of Morinda citrifolia fruit ethanol extract applied to the skin of rats daily for 28 days, thirty female Sprague Dawley rats were grouped into five groups (n=5 rats for each group) for each study. The experimental period was set at 14 days and 28 days for acute and subacute dermal toxicity, respectively. Rats in the treatment groups were topically applied with the plant extract at three different doses; 2.5%, 5% and 10% on a shaved area of …


Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan Mar 2021

Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan

The Thai Journal of Veterinary Medicine

Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …


Evaluation Of Protection Efficiency Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus In Broiler Chickens: Comparative In Field And In Laboratory Studies, Serageldeen Sultan, Marwa Hamed, Nabila Osman Mar 2021

Evaluation Of Protection Efficiency Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus In Broiler Chickens: Comparative In Field And In Laboratory Studies, Serageldeen Sultan, Marwa Hamed, Nabila Osman

The Thai Journal of Veterinary Medicine

This study was conducted to evaluate the protective efficiency of different vaccines and vaccination programs against Newcastle disease virus (NDV) in chickens, in the field and the laboratory. In the field, three commercial chicken farms (LA, LB, and QC) used different types of live vaccines and vaccination programs via drinking water (DW) were serologically estimated by hemagglutination inhibition (HI) assay to detect the level of circulating antibodies against NDV. The laboratory study was carried out to assess whether the types of vaccine and their route of administration influence the HI level and viral shedding. Chicken groups (G1-G5) except for the …


Detection Of Bacillus Cereus And Gram-Negative Bacteria Communities In Commercial Sesame In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita Mar 2021

Detection Of Bacillus Cereus And Gram-Negative Bacteria Communities In Commercial Sesame In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita

The Thai Journal of Veterinary Medicine

Sesame seeds are used in many traditional Japanese foods for their flavor and taste but commercial sesame can be highly contaminated with bacteria. We therefore examined the bacterial populations, including Bacillus spp., Enterobacteriaceae bacteria and standard plate count bacteria in sesame samples purchased from markets in Japan. A total of 8 sesame samples were tested, of which 4 (50.0%) harbored 4.3–5.6 log cfu/g standard plate count and 2.7–4.3 log cfu/g Enterobacteriaceae bacilli. Pantoea dispersa, P. septica and P. agglomerans were identified by a MALDI-TOF MSbased test. One (12.5%) sample harbored 3.6 log cfu/g Bacillus cereus, but this strain lacked the …


Study Of The Mechanism Of Fomes Officinalis Polysaccharides Inhibiting The Contraction Of The Isolated Duodenum In Mice Through The Myenteric Plexus, Ailong Sha, Haiyan Hao Mar 2021

Study Of The Mechanism Of Fomes Officinalis Polysaccharides Inhibiting The Contraction Of The Isolated Duodenum In Mice Through The Myenteric Plexus, Ailong Sha, Haiyan Hao

The Thai Journal of Veterinary Medicine

The objectives of this study were to observe the effect of different concentrations of Fomes officinalis polysaccharides (FOPs) on the contractile activity of the isolated duodenal smooth muscle in mice and explore its mechanism. The contractility of the isolated duodenum was recorded by the Medlab biological signal acquisition and processing system before and after administration and the effects of FOPs-treated groups at low, medium and high dose (5, 10 and 20 mg/mL) on the contractile frequency and amplitude of the intestines were observed. Adrenaline hydrochloride (AD) and CaCl2 were selected respectively to be co-incubated with the high dose FOPs to …


Evaluation Of Tear Quantity And Quality Treated With Gintonin Eye Drops In Labrador Retrievers And English Springer Spaniels, Ji-Seung Choi, Joon-Young Kim, Soon-Wuk Jeong Mar 2021

Evaluation Of Tear Quantity And Quality Treated With Gintonin Eye Drops In Labrador Retrievers And English Springer Spaniels, Ji-Seung Choi, Joon-Young Kim, Soon-Wuk Jeong

The Thai Journal of Veterinary Medicine

The aims of this study were to determine the relationship between tear osmolarity (TO), Schirmer tear test-1 (STT- 1) and tear film break-up time (TBUT) values in canines and to determine the impact of eye drops containing gintonin - a ginseng-derived lysophosphatidic acid (LPA) receptor ligand - on these indices. Fourteen canines (four English springer spaniels and ten Labrador retrievers) were randomized into two groups: the control group received only placebo eye drops (carboxymethylcellulose), while the experimental group received carboxymethylcellulose eye drops containing 5 µg of gintonin. Treatment was applied twice per day for a period of four weeks and …